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Phage Display: A Practical Approach (Practical Approach
Series)
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business account Series: Practical Approach Series (Book 266) Hardcover: 360 pages Publisher: Oxford
University Press; 1 edition (May 6, 2004) Language: English ISBN-10: 0199638748 ISBN-13: 978-
0199638741 Product Dimensions: 9.8 x 1 x 7.5 inches
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Book Description
Phage display has become established as a powerful protein engineering method for identifying
polypeptides with novel properties, and altering the properties of existing ones. Although the
technique is widely used in biological research and drug discovery, it remains technically
challenging, and new applications and procedures continue to evolve. Phage Display - A Practical
Approach is an up-to-date, comprehensive and integrated experimental guide to the technique,
useful for novice and expert alike. The book aims to enable researchers to design and undertake
all aspects of a phage display project, from designing an experimental strategy and constructing a
library to performing selections and analyzing the results. An introductory chapter provides an
overview of phage biology and phage display, including guidelines for planning a successful
phage display experiment. Individual chapters provide protocols for constructing libraries using
oligonucleotide-directed mutagenesis or DNA recombination, performing binding selections, and
analyzing the binding activities of selected phage clones. Separate chapters then cover common
applications, including selection of ligands from peptide libraries, generation of phage antibody
libraries and isolation and optimization of antibodies, selection of DNA binding proteins, and
expression cloning using cDNA display. Further chapters describe alternative selection strategies,
such as selection using immune sera, selection based on enzymatic activity or protein stability,
and selection in vivo. Protocols and chapters are extensively cross-referenced, allowing readers to
move beyond the specific examples given to customize the procedures to their own protein or
selection system of interest. Written by experts in the field, Phage Display - A Practical Approach
provides a comprehensive guide to the design and execution of phage display projects, for all
those using the technique in basic research and drug discovery.

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