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Modern Research <strong>and</strong> Educational Topics <strong>in</strong> Microscopy.<br />

A. Méndez-Vilas <strong>and</strong> J. Díaz (Eds.) ©FORMATEX 2007<br />

_______________________________________________________________________________________________<br />

<strong>CONTENTS</strong><br />

VOL. 1<br />

Introduction........................................................................................................................... XV<br />

<strong>Applications</strong> <strong>in</strong> <strong>Biology</strong> <strong>and</strong> Medic<strong>in</strong>e<br />

Approaches for Investigat<strong>in</strong>g Mechanobiological Dynamics <strong>in</strong> Liv<strong>in</strong>g Cells with<br />

Fluorescence <strong>and</strong> Atomic Force Microscopies<br />

A.E. Pell<strong>in</strong>g, B. M. Nicholls, Y.R. Silberberg, <strong>and</strong> M.A. Horton..........................................<br />

Challenges <strong>and</strong> Approaches - Prob<strong>in</strong>g Tumor Cell Invasion by Atomic Force<br />

Microscopy<br />

T. Ludwig, R. Kirmse <strong>and</strong> Kate Poole...................................................................................<br />

Study of Cancer Cells Used Atomic Force Microscopy<br />

K. Tomankova, H. Kolarova, M. Vujtek <strong>and</strong> H. Zapletalova................................................<br />

Atomic Force Imag<strong>in</strong>g of Ocular Tissues: morphological study of healthy <strong>and</strong><br />

cataract lenses<br />

A. Antunes, F. V. Gozzo, M. I. Borella, M. Nakamura, A. M. V. Safatle, P. S. M. Barros<br />

<strong>and</strong> H. E. Toma………………………………………………………………………………<br />

Imag<strong>in</strong>g of Bone Ultrastructure us<strong>in</strong>g Atomic Force Microscopy<br />

P. J. Thurner, E. Oroudjev, R. Jungmann, C. Kreutz, J. H. K<strong>in</strong>dt, G. Schitter, T. O.<br />

Okouneva, M. E. Lauer, G. E. Fantner, H. G. Hansma, <strong>and</strong> P. K. Hansma............................<br />

Cell Surface Membrane Remodell<strong>in</strong>g <strong>and</strong> Mitochondrial Remodell<strong>in</strong>g <strong>in</strong> Live<br />

Neurons: Practical Approaches for Study<strong>in</strong>g Dynamic Processes Us<strong>in</strong>g Confocal<br />

Microscopy<br />

N. Shulyakova, P. Kaifosh, D. Diec, <strong>and</strong> L.R. Mills...............................................................<br />

Cell-surface <strong>in</strong>teraction <strong>in</strong> biomedical implants assessed by simultaneous<br />

fluorescence <strong>and</strong> reflection confocal microscopy<br />

J. Vilches, J.I. Vilches-Perez <strong>and</strong> M. Salido..........................................................................<br />

The comb<strong>in</strong>ed application of AFM <strong>and</strong> LSCM: Chang<strong>in</strong>g the way we look at<strong>in</strong>nate<br />

immunity<br />

E. L. Adams <strong>and</strong> K. J. Czymmek............................................................................................<br />

Electron Microscopy Visualization of the Cell Surface of Trypanosomatids<br />

W. de Souza.............................................................................................................................<br />

Extend<strong>in</strong>g the Explanatory Power of Live Cell Imag<strong>in</strong>g by computationally<br />

modell<strong>in</strong>g the Execution of Apoptotic Cell Death<br />

H. Huber, G. Gomez Estrada, H. Dussmann, C. O’Connor <strong>and</strong> M. Rehm............................<br />

3-10<br />

11-22<br />

23-28<br />

29-36<br />

37-48<br />

49-59<br />

60-67<br />

68-76<br />

77-87<br />

88-99<br />

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Modern Research <strong>and</strong> Educational Topics <strong>in</strong> Microscopy.<br />

_______________________________________________________________________________________________<br />

©FORMATEX 2007<br />

A. Méndez-Vilas <strong>and</strong> J. Díaz (Eds.)<br />

In vivo <strong>and</strong> <strong>in</strong> vitro Approaches for the Study of Adult Neurogenesis <strong>in</strong> Light,<br />

Confocal, <strong>and</strong> Electron Microscopy<br />

N. Canalia, M. Armentano, G. Ponti <strong>and</strong> L. Bonfanti............................................................<br />

Microscopy studies on uncultivated magnetotactic bacteria<br />

T. S. Silveira, J. L. Mart<strong>in</strong>s, K. T. Silva, F. Abreu <strong>and</strong> U. L<strong>in</strong>s.............................................<br />

Scann<strong>in</strong>g Electron Microscopy <strong>and</strong> Transmission Electron Microscopy of Mollicutes:<br />

Challenges <strong>and</strong> Opportunities<br />

C. T. K.-H. Stadtländer............................................................................................................<br />

Non-Invasive Fourier Transform Infrared Microspectroscopy <strong>and</strong> Imag<strong>in</strong>g<br />

Techniques: Basic Pr<strong>in</strong>ciples <strong>and</strong> <strong>Applications</strong><br />

P. Garidel <strong>and</strong> M. Boese..........................................................................................................<br />

Contribution of Electron Microscopy to the Study of the Nuclear Pore Complex<br />

Structure, Composition, <strong>and</strong> Function<br />

N. Panté...................................................................................................................................<br />

Bright Solutions to Get Sharp Images: Confocal <strong>and</strong> Two- Photon Fluorescence<br />

Microscopy <strong>and</strong> the Pros <strong>and</strong> Cons of New Multifocal Approaches<br />

R. Kurtz...................................................................................................................................<br />

Microscopy techniques <strong>and</strong> the study of synapses<br />

E. Perez-Costas, M. Melendez-Ferro <strong>and</strong> R.C. Roberts……………………………………..<br />

Microscopic Investigations <strong>in</strong> Neurodegenerative Diseases<br />

R. J. Castellani, B. A. Alexiev, D. Phillips, G. Perry <strong>and</strong> M. A. Smith..................................<br />

Study<strong>in</strong>g the temperature-dependent events of live cells under confocal <strong>and</strong> epifluorescence<br />

microscopy us<strong>in</strong>g a solid-state heat<strong>in</strong>g/cool<strong>in</strong>g system<br />

Zu-Han Huang, Huei-Hsuan Cheng, Huei-In Wu, Sh<strong>in</strong>-Hua Tseng <strong>and</strong> Yen-Chung Chang.<br />

Live imag<strong>in</strong>g genetically-encoded fluorescent prote<strong>in</strong>s <strong>in</strong> embryonic stem cells us<strong>in</strong>g<br />

confocal microscopy<br />

J. Artus <strong>and</strong> A.-K. Hadjantonakis............................................................................................<br />

Correlative Fluorescence- <strong>and</strong> Scann<strong>in</strong>g, Transmission Electron Microscopy for<br />

Biomolecular Investigation<br />

K. Jahn, D. Barton <strong>and</strong> F. Braet..............................................................................................<br />

Immunolocalization of maize transglutam<strong>in</strong>ase <strong>and</strong> its substrates <strong>in</strong> plant cells <strong>and</strong> <strong>in</strong><br />

Escherichia coli transformed cells<br />

M. Santos, E.Villalobos, P.Carvajal-Vallejos, E. Barberá, A. Campos <strong>and</strong> J.M.Torné…….<br />

Visualization of the real microarchitecture of glycoprote<strong>in</strong> matrices with scann<strong>in</strong>g<br />

electron microscopy<br />

G. Familiari, M. Relucenti, A. Familiari, E. Battaglione, G. Franchitto <strong>and</strong> R. Heyn............<br />

100-110<br />

111-121<br />

122-131<br />

132-143<br />

144-153<br />

154-163<br />

164-170<br />

171-182<br />

183-189<br />

190-202<br />

203-211<br />

212-223<br />

224-228<br />

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Modern Research <strong>and</strong> Educational Topics <strong>in</strong> Microscopy.<br />

A. Méndez-Vilas <strong>and</strong> J. Díaz (Eds.) ©FORMATEX 2007<br />

_______________________________________________________________________________________________<br />

Use of st<strong>and</strong>ard fluorescence microscopy to assess modifications <strong>in</strong> the plasma<br />

membrane potential <strong>and</strong> <strong>in</strong> the <strong>in</strong>tracellular concentration of <strong>in</strong>organic ions <strong>in</strong><br />

cultured cells<br />

S. Chifflet <strong>and</strong> J.A. Hernández................................................................................................<br />

Imag<strong>in</strong>g biological structures with a proton microprobe<br />

T. P<strong>in</strong>heiro, M.D.Ynsa <strong>and</strong> L.C.Alves....................................................................................<br />

Macromolecular Synthesis <strong>in</strong> Hepatocyte Mitochondria of Ag<strong>in</strong>g Mice as Revealed<br />

by Electron Microscopic Radioautography I: Nucleic Acid Synthesis<br />

T. Nagata.................................................................................................................................<br />

Macromolecular Synthesis <strong>in</strong> Hepatocyte Mitochondria of Ag<strong>in</strong>g Mice as Revealed<br />

by Electron Microscopic Radioautography II : Prote<strong>in</strong> Synthesis<br />

T. Nagata.................................................................................................................................<br />

Size Estimation of Prote<strong>in</strong> Clusters <strong>in</strong> the Nanometer Range by Us<strong>in</strong>g Spatially<br />

Modulated Illum<strong>in</strong>ation Microscopy<br />

U. J. Birk, I. Upmann, D. Toomre, C. Wagner <strong>and</strong> C. Cremer...............................................<br />

Transglutam<strong>in</strong>ase activity <strong>and</strong> localization dur<strong>in</strong>g microspore <strong>in</strong>duction <strong>in</strong> maize<br />

M. Santos, J. Alché , M.I. Rodríguez-García <strong>and</strong> J.M.Torné………………………………..<br />

A Thous<strong>and</strong> Prote<strong>in</strong>s of Light: 15 Years of Advances <strong>in</strong> Fluorescent Prote<strong>in</strong>s<br />

G. McNamara <strong>and</strong> C.A. Boswell.............................................................................................<br />

Us<strong>in</strong>g Total Internal Reflection Fluorescence Microscopy, DNA Curta<strong>in</strong>s, <strong>and</strong><br />

Quantum Dots to Investigate Prote<strong>in</strong>-DNA Interactions at the S<strong>in</strong>gle-molecule Level<br />

M-L. Visnapuu, D. Duzdevich <strong>and</strong> E. C. Greene....................................................................<br />

Fluorescence Resonance Energy Transfer us<strong>in</strong>g molecular beacon as a probe, a new<br />

approach for <strong>in</strong> vivo macromolecular <strong>in</strong>teraction study<br />

S. Ganguly...............................................................................................................................<br />

Electron-microscopic <strong>and</strong> genetic dissection of arthropod cuticle differentiation<br />

H. Schwarz <strong>and</strong> B. Moussian..................................................................................................<br />

Microscopy <strong>and</strong> egg morphology of Mayflies<br />

N. Ubero-Pascal <strong>and</strong> M.A. Puig..............................................................................................<br />

Role of Scann<strong>in</strong>g Electron Microscopy <strong>in</strong> Underst<strong>and</strong><strong>in</strong>g Insect Corneal Nipple <strong>and</strong><br />

Other Structures<br />

S. Dey......................................................................................................................................<br />

Antennal sensilla of hymenopteran parasitic wasps: variations l<strong>in</strong>ked to host<br />

exploitation behavior<br />

J. van Baaren, G. Boiv<strong>in</strong>, D. Bourdais <strong>and</strong> O. Roux...............................................................<br />

229-236<br />

237-244<br />

245-258<br />

259-271<br />

272-279<br />

280-286<br />

287-296<br />

297-308<br />

309-315<br />

316-325<br />

326-335<br />

336-344<br />

345-352<br />

VII


Modern Research <strong>and</strong> Educational Topics <strong>in</strong> Microscopy.<br />

_______________________________________________________________________________________________<br />

©FORMATEX 2007<br />

A. Méndez-Vilas <strong>and</strong> J. Díaz (Eds.)<br />

The application of confocal microscopy <strong>and</strong> 3D imag<strong>in</strong>g software <strong>in</strong> Functional,<br />

Evolutionary, <strong>and</strong> Developmental Zoology: reconstruct<strong>in</strong>g myo- <strong>and</strong> neurogenesis <strong>in</strong><br />

space <strong>and</strong> time<br />

A. Wann<strong>in</strong>ger..........................................................................................................................<br />

Comparative study of the impact of the act<strong>in</strong> cytoskeleton on cell shape <strong>and</strong><br />

membrane surface <strong>in</strong> mammalian cells <strong>in</strong> response to act<strong>in</strong> tox<strong>in</strong>s<br />

F. Lázaro-Diéguez <strong>and</strong> G. Egea……………………………………………………………..<br />

Chromosomes <strong>in</strong> Focus: Basic Cytogenetics, Light Microscopy <strong>and</strong> the Case of<br />

Neotropical Fish<br />

P.R.A.M Affonso, V S. Mir<strong>and</strong>a, A. S. Medrado, U. P Jacob<strong>in</strong>a, J. A. Bitencourt, J. S.<br />

Almeida <strong>and</strong> P. L. S. Carneiro.................................................................................................<br />

The Nuclear Area Factor (NAF): a measure for cell apoptosis us<strong>in</strong>g microscopy <strong>and</strong><br />

image analysis<br />

M. A. DeCoster.......................................................................................................................<br />

Study of the Mechanisms of Action of Medic<strong>in</strong>es <strong>in</strong> the Immune System Us<strong>in</strong>g<br />

Microscopy<br />

C. Camargo de Oliveira <strong>and</strong> D. de Freitas Buchi……………………………………………<br />

Prob<strong>in</strong>g the Structure <strong>and</strong> Function of Mammalian Sperm us<strong>in</strong>g Optical <strong>and</strong><br />

Fluorescence Microscopy<br />

J. Ramalho-Santos, A. Amaral, A.P. Sousa, A.S. Rodrigues, L. Mart<strong>in</strong>s, M. Baptista, P.C.<br />

Mota, R. Tavares, S. Amaral <strong>and</strong> S. Gamboa……………………………………………….<br />

Super-Quiet Microfluorometry: Examples of Tumor Cell Metabolic Dynamics<br />

A.J. Clark <strong>and</strong> H.R. Petty........................................................................................................<br />

Real-time visualization of develop<strong>in</strong>g viral <strong>in</strong>fection <strong>in</strong> fibroblasts<br />

K.L. Lathrop <strong>and</strong> A. Eisfeld....................................................................................................<br />

Morphology of the develop<strong>in</strong>g fetal lung – the rabbit experimental model<br />

X I. Roubliova , J. M. Biard, L. Ophalvens, D. Gallot, J.C. Jani , E.K.Verbeken, C.P. Van<br />

de Ven, D. Tibboel <strong>and</strong> J.A. Deprest.......................................................................................<br />

3D visualization <strong>and</strong> analysis of cell-matrix transformations <strong>in</strong> whole-mount <strong>and</strong> live<br />

embryos us<strong>in</strong>g confocal <strong>and</strong> multi-photon microscopy<br />

G.G. Mart<strong>in</strong>s, P. Rifes, R. Amândio, P. Camp<strong>in</strong>ho, I. Palmeirim <strong>and</strong> S. Thorste<strong>in</strong>sdóttir.....<br />

Utilization of the Laser Induced Plasma Spectroscopy for monitor<strong>in</strong>g of the metal<br />

accumulation <strong>in</strong> plant tissues with high spatial resolution<br />

J. Kaiser, M. Galiová, K. Novotný, L. Reale, K. Stejska, O. Samek, R. Mal<strong>in</strong>a, K.<br />

Páleníková, V. Adam <strong>and</strong> R. Kizek.........................................................................................<br />

Quantitative Microscopic Analysis of Histological Sections of Bra<strong>in</strong> Tissue<br />

R. A. Armstrong......................................................................................................................<br />

353-361<br />

362-369<br />

370-377<br />

378-384<br />

385-393<br />

394-402<br />

403-408<br />

409-416<br />

417-425<br />

426-433<br />

434-441<br />

442-452<br />

VIII


Modern Research <strong>and</strong> Educational Topics <strong>in</strong> Microscopy.<br />

A. Méndez-Vilas <strong>and</strong> J. Díaz (Eds.) ©FORMATEX 2007<br />

_______________________________________________________________________________________________<br />

Ultrastructural Changes In Microcoleus chthonoplastes Grow<strong>in</strong>g In the Presence of<br />

Crude Oil. <strong>Applications</strong> for Ecological Studies<br />

E. Diestra, I. Esteve, O. Castell <strong>and</strong> A. Solé........................................................................... 453-460<br />

Correlation Between flow Cytometry <strong>and</strong> Transmission Electron Microscopy<br />

A. Alvarez, F. Goñi de Cerio <strong>and</strong> E. Hilario………………………………………………... 461-468<br />

VOL. 2<br />

Introduction........................................................................................................................... XV<br />

<strong>Applications</strong> <strong>in</strong> Physical/Chemical Sciences<br />

Scann<strong>in</strong>g Force Microscopic Study of Surface Structure <strong>and</strong> Surface Properties of<br />

Organosilane Monolayers<br />

T. Koga <strong>and</strong> A. Takahara........................................................................................................<br />

Nano-Structur<strong>in</strong>g <strong>and</strong> Molecular Doma<strong>in</strong> Organizations <strong>in</strong> Lipid-Prote<strong>in</strong><br />

Membranous Interfaces<br />

K. Nag, A.K. P<strong>and</strong>a, R. Devraj <strong>and</strong> M. Fritzen-Garcia...........................................................<br />

Nanolubrication studied by Contact-Mode Atomic Force Microscopy<br />

R. Buzio <strong>and</strong> U. Valbusa.........................................................................................................<br />

Experimental contribution to the underst<strong>and</strong><strong>in</strong>g of wett<strong>in</strong>g of solid surfaces at the<br />

meso- <strong>and</strong> nano-scale us<strong>in</strong>g dynamic AFM<br />

A.B. Jódar-Reyes, A. Méndez-Vilas <strong>and</strong> M.L. González-Martín...........................................<br />

Usage of AFM, SEM <strong>and</strong> TEM for the research of carbon nanotubes<br />

K. Safarova, A. Dvorak, R. Kub<strong>in</strong>ek <strong>and</strong> M. Vujtek A. Rek..................................................<br />

Atomic Force Microscopy as a tool for unravell<strong>in</strong>g the relationship between<br />

morphology <strong>and</strong> growth dynamics of organic semiconductors<br />

M. Campione, M. Moret <strong>and</strong> A. Sassella................................................................................<br />

AFM monitor<strong>in</strong>g of size changes dur<strong>in</strong>g thermal syntheses of ferric oxide<br />

nanoparticles<br />

M. Vůjtek, R. Kubínek, R. Zbořil <strong>and</strong> L. Machala.................................................................<br />

Various approaches to control solid/solid wett<strong>in</strong>g self-assembly of organic<br />

semiconductors with STM<br />

F. Trixler <strong>and</strong> W.M. Heckl......................................................................................................<br />

Dephas<strong>in</strong>g of charge <strong>and</strong> sp<strong>in</strong> <strong>in</strong> semiconductor quantum dots<br />

W. Jacak, J. Krasnyj, <strong>and</strong> L. Jacak.........................................................................................<br />

471-482<br />

483-490<br />

491-499<br />

500-512<br />

513-519<br />

520-527<br />

528-533<br />

534-541<br />

542-549<br />

IX


Modern Research <strong>and</strong> Educational Topics <strong>in</strong> Microscopy.<br />

_______________________________________________________________________________________________<br />

©FORMATEX 2007<br />

A. Méndez-Vilas <strong>and</strong> J. Díaz (Eds.)<br />

Investigation of the nanostructured surface of s<strong>in</strong>gle-crystal silicon by the method of<br />

scann<strong>in</strong>g tunnel spectroscopy<br />

S.P. Kulyk, M.M. Melnichenko, K.V. Svezhentsova <strong>and</strong> O.M. Shmyryeva..........................<br />

Electrically characterization of Group III-Nitride nanocolumns with Scann<strong>in</strong>g Force<br />

Microscopy<br />

M. Niebelschütz, V. Cimalla, O. Ambacher, T. Machleidt, K.-H. Franke, J. Ristic, J.<br />

Gr<strong>and</strong>al, M.A. Sánchez-García <strong>and</strong> E. Calleja........................................................................<br />

Field Emission Scann<strong>in</strong>g Electron Microscopy for Structural Characterization of 3D<br />

Gold Nanoparticle Superlattices<br />

H. Yao <strong>and</strong> K. Kimura.............................................................................................................<br />

UV-VIS <strong>and</strong> TEM assessment of morphological features of silver nanoparticles from<br />

phosphate glass matrices<br />

L. Baia <strong>and</strong> S. Simon...............................................................................................................<br />

Evaluation of electronic <strong>and</strong> geometric properties of nanoparticles us<strong>in</strong>g X-PEEM<br />

O. Seifarth...............................................................................................................................<br />

Exam<strong>in</strong>ation of dent<strong>in</strong> surface us<strong>in</strong>g AFM <strong>and</strong> SEM<br />

R. Kub<strong>in</strong>ek, Z. Zapletalova, M. Vujtek, R. Novotný, H. Kolarova <strong>and</strong> H. Chmelickova......<br />

TEM analysis of the early m<strong>in</strong>eralization process of mantle dent<strong>in</strong><br />

P. Dechichi, C. Christian Gomes Moura, A. Wilsson de Almeida Filho <strong>and</strong> J. Carlos<br />

Gabrielli Biffi………………………………………………………………………………..<br />

Spatial visualization of thermally sprayed microstructure based on light microscopy<br />

P. Ctibor <strong>and</strong> R. Lechnerova...................................................................................................<br />

Analytical electron microscopy of tremolite<br />

K.N. Bozhilov <strong>and</strong> D.M. Jenk<strong>in</strong>s............................................................................................<br />

Investigat<strong>in</strong>g morphological changes on octadecyl modifiedsilicas by SEM <strong>and</strong> AFM<br />

R. Brambilla, F. Silveira <strong>and</strong> J.H.Z. dos Santos……………………………………………..<br />

Importance of Transmission Electron Microscopy for Carbon Nanomaterials<br />

Research<br />

P.R. Somani <strong>and</strong> M. Umeno....................................................................................................<br />

Discover<strong>in</strong>g the True Morphology of Amphibole M<strong>in</strong>erals: Complementary TEM<br />

<strong>and</strong> FESEM Characterization of Particles <strong>in</strong> Mixed M<strong>in</strong>eral Dust<br />

K. E. Harris, K. L. Bunker, B. R. Strohmeier, R. Hoch <strong>and</strong> R. J. Lee....................................<br />

S<strong>in</strong>gle-Molecule Fluorescence Imag<strong>in</strong>g Techniques for the Detection of Reactive<br />

Oxygen Species<br />

T. Tachikawa <strong>and</strong> T. Majima..................................................................................................<br />

550-559<br />

560-567<br />

568-575<br />

576-583<br />

584-592<br />

593-598<br />

599-605<br />

606-615<br />

616-625<br />

626-633<br />

634-642<br />

643-650<br />

651-659<br />

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Modern Research <strong>and</strong> Educational Topics <strong>in</strong> Microscopy.<br />

A. Méndez-Vilas <strong>and</strong> J. Díaz (Eds.) ©FORMATEX 2007<br />

_______________________________________________________________________________________________<br />

Scann<strong>in</strong>g Electron Microscopy Investigation of Lead-Free High Refractive Index<br />

Glass Prepared from Local S<strong>and</strong> Used for Restoration <strong>and</strong> Conservation<br />

P. Dararutana <strong>and</strong> N. Sirikulrat...............................................................................................<br />

SEM <strong>and</strong> TEM studies <strong>in</strong> alloys developed by mechanical alloy<strong>in</strong>g<br />

J.J. Suñol <strong>and</strong> L. Escoda..........................................................................................................<br />

Scann<strong>in</strong>g Electron Microscopic Characterization of Copper (II) Phthalocyan<strong>in</strong>e<br />

Nanocrystallites Th<strong>in</strong> Films Deposited on Technologically Important Substrates<br />

B. Mallik <strong>and</strong> S. Karan............................................................................................................<br />

Surface Morphological Study of Low Temperature Plasma Treated Wool – A Time<br />

Dependence Study<br />

C.W. Kan.................................................................................................................................<br />

Fluorescence <strong>and</strong> Scann<strong>in</strong>g Electron Microscopy of Chitosan/DNA Nanoparticles for<br />

Biological <strong>Applications</strong><br />

A. Masotti, F. Mar<strong>in</strong>o, G. Ortaggi <strong>and</strong> C. Palocci...................................................................<br />

A Novel “In-situ-track<strong>in</strong>g” Approach for Evaluat<strong>in</strong>g Microstructural Variations<br />

Us<strong>in</strong>g SEM, EDS <strong>and</strong> EBSD <strong>and</strong> Its <strong>Applications</strong> <strong>in</strong> Materials Science<br />

C. Pan, Y. Huang <strong>and</strong> Q. Fu....................................................................................................<br />

Morphology Variations of Polypropylene<br />

J. Výchopňová, R. Čermák <strong>and</strong> M. Obadal.............................................................................<br />

Morphological Transition of Isotactic Polybutene-1 Tetragonal Crystals: Optical <strong>and</strong><br />

Transmission Electron Microscopy Observation<br />

M. Yamashita <strong>and</strong> T. Takahashi..............................................................................................<br />

Nanostructure of starch high-pressure treated granules discovered by low<br />

temperature scann<strong>in</strong>g electron microscopy<br />

A.D. Mol<strong>in</strong>a-García, E. Horridge, P.D. Sanz <strong>and</strong> M.N. Mart<strong>in</strong>o……………………………<br />

Nanoscale Structure of Cellulosic Materials: Challenges <strong>and</strong> Opportunities for AFM<br />

A.T. Paiva, S.M. Sequeira, D.V. Evtugu<strong>in</strong>, A.L. Kholk<strong>in</strong> <strong>and</strong> I.Portugal..............................<br />

Atomic Force Microscope Nano<strong>in</strong>dentations to Reliably Measure the Young’s<br />

Modulus of Soft Matter<br />

D. Tranchida, Z. Kiflie <strong>and</strong> S. Piccarolo…………………………………………………….<br />

The Atomic Force Spectroscopy as a Tool to Investigate Surface Forces: Basic<br />

Pr<strong>in</strong>ciples <strong>and</strong> <strong>Applications</strong><br />

F.L. Leite, L.H.C. Mattoso, O.N. Oliveira Jr <strong>and</strong> P.S.P. Herrmann Jr....................................<br />

660-665<br />

666-670<br />

671-682<br />

683-689<br />

690-696<br />

697-703<br />

704-712<br />

713-718<br />

719-725<br />

726-733<br />

Techniques<br />

737-746<br />

747-757<br />

XI


Modern Research <strong>and</strong> Educational Topics <strong>in</strong> Microscopy.<br />

_______________________________________________________________________________________________<br />

©FORMATEX 2007<br />

A. Méndez-Vilas <strong>and</strong> J. Díaz (Eds.)<br />

A Guide for Atomic Force Microscopy Analysis of Soft-Condensed Matter<br />

M. Raposo, Q. Ferreira <strong>and</strong> P.A. Ribeiro................................................................................<br />

Scann<strong>in</strong>g Probe Microscopy-Based Nanofabrication for Emerg<strong>in</strong>g <strong>Applications</strong><br />

X. Liu <strong>and</strong> H. Zhang................................................................................................................<br />

Explor<strong>in</strong>g the high pressure SEM<br />

C. Mathieu, L Khouchaf <strong>and</strong> A Kadoun.................................................................................<br />

How to study biological samples by FIB/SEM?<br />

M. Milani, D. Drobne <strong>and</strong> Francesco Tatti.............................................................................<br />

Very Low Energy Scann<strong>in</strong>g Electron Microscopy<br />

I. Müllerová <strong>and</strong> L. Frank.......................................................................................................<br />

The magnetic force microscopy <strong>and</strong> its capability for nanomagnetic studies - The<br />

short compendium<br />

A. Hendrych, R. Kubínek <strong>and</strong> A.V. Zhukov...........................................................................<br />

Visualisation of Small Fluid Droplets on Biological <strong>and</strong> Artificial Surfaces Us<strong>in</strong>g the<br />

Cryo-SEM Approach<br />

S.N. Gorb, D. Voigt <strong>and</strong> E.V. Gorb........................................................................................<br />

Spherical AFM Probes for Adhesion Force Measurements on Metal S<strong>in</strong>gle Crystals<br />

B. Stegemann, H. Backhaus, H. Kloss <strong>and</strong> E. Santner............................................................<br />

Near-Field Optical Microscopy of Surface Plasmon Polaritons Nano-Optics<br />

Víctor Coello...........................................................................................................................<br />

Semiconductor nanocrystals <strong>and</strong> fluorescence microscopy <strong>in</strong> biological label<strong>in</strong>g<br />

P.M.A. Farias, B.S. Santos, A.Fontes <strong>and</strong> C.L. Cesar………………………………………<br />

Fluorescence Correlation Spectroscopy of Liv<strong>in</strong>g Cells<br />

G. Vereb, L. Ujlaky-Nagy, E. Friedländer G. Vámosi <strong>and</strong> J. Szöllősi....................................<br />

Fluorescence Correlation Spectroscopy: an Experimentalist’s View of the Basics<br />

G. Jung....................................................................................................................................<br />

L<strong>in</strong>ear fluorescence unmix<strong>in</strong>g <strong>in</strong> cell biological research<br />

B. Kraus, M. Ziegler <strong>and</strong> H. Wolff.........................................................................................<br />

High resolution Near-field Fluorescence Microscopy: from pr<strong>in</strong>ciples to applications<br />

<strong>in</strong> cell biology<br />

A. Cambi, C.G. Figdor <strong>and</strong> M.F. Garcia-Parajo…………………………………………….<br />

Two-Photon Fluorescence Microscopy: Basic Pr<strong>in</strong>ciples, Advantages <strong>and</strong> Risks<br />

S.J. Mulligan <strong>and</strong> B.A. MacVicar...........................................................................................<br />

758-769<br />

770-778<br />

779-786<br />

787-794<br />

795-804<br />

805-811<br />

812-819<br />

820-827<br />

828-839<br />

840-847<br />

848-854<br />

855-862<br />

863-872<br />

873-880<br />

881-889<br />

XII


Modern Research <strong>and</strong> Educational Topics <strong>in</strong> Microscopy.<br />

A. Méndez-Vilas <strong>and</strong> J. Díaz (Eds.) ©FORMATEX 2007<br />

_______________________________________________________________________________________________<br />

Colours Count: how the Challenge of Fluorescence was solved <strong>in</strong> Confocal<br />

Microscopy<br />

R. Borl<strong>in</strong>ghaus.........................................................................................................................<br />

Software-based three dimensional reconstructions <strong>and</strong> enhancements of focal depth<br />

<strong>in</strong> microphotographic images<br />

J. Piper.....................................................................................................................................<br />

Digital Deconvolution Microscopy: Development, Evaluation <strong>and</strong> Utilization <strong>in</strong> 3D<br />

quantitative studies of E-cadher<strong>in</strong> expression <strong>in</strong> sk<strong>in</strong> of Bufo arenarun tadpoles<br />

J.F. Adur, J.E. Diaz-Zamboni, N.B. Vicente, M.F. Izaguirre <strong>and</strong> V.H. Casco.......................<br />

Concurrent 3-D Visualization of Multiple Microscopic Structures<br />

S.J. Rehorek <strong>and</strong> T.D. Smith...................................................................................................<br />

Pr<strong>in</strong>ciples of automatic vision systems for track<strong>in</strong>g elongated microorganisms<br />

N. Babaii Rizv<strong>and</strong>i, D. Ochoa, A. Pizurica <strong>and</strong> W. Philips....................................................<br />

Registration Decisions dur<strong>in</strong>g 3D Medical Volume Reconstructions from Confocal<br />

Laser Scann<strong>in</strong>g Microscopy<br />

P. Bajcsy <strong>and</strong> Sang-Chul Lee..................................................................................................<br />

An <strong>in</strong>troduction to low dose electron tomography- from specimen preparation to<br />

data collection<br />

G. Rh. Owen <strong>and</strong> D.L.Stokes..................................................................................................<br />

Temperature controlled Microscopy<br />

A. Holz<strong>in</strong>ger............................................................................................................................<br />

An <strong>in</strong>troduction to diffractive tomographic microscopy<br />

O. Haeberlé, A. Sentenac <strong>and</strong> H. Giovann<strong>in</strong>i..........................................................................<br />

Scann<strong>in</strong>g ion conductance microscopy–a tool to <strong>in</strong>vestigate electrolyte-nonconductor<br />

<strong>in</strong>terfaces<br />

P. Happel , F. Wehner <strong>and</strong> I. D. Dietzel..................................................................................<br />

In Situ X-Ray Microscopy at High Temperature <strong>and</strong> Pressure<br />

B. Ménez, H. Bureau, J. Cauzid, V. Malavergne, A. Somogyi, A. Simionovici, M. Munoz,<br />

L. Avoscan, C. Rommevaux-Jest<strong>in</strong> <strong>and</strong> B. Gouget.................................................................<br />

Equipment for coupl<strong>in</strong>g a digital camera to a optical microscopy<br />

A.P. de Novaes, P.S. de Paula Herrmann Jr., R. Bernardes Filho <strong>and</strong> F.M. Benetti..............<br />

Virtual Microscopy <strong>in</strong> Medical Images: a Survey<br />

E. Romero, F. Gómez <strong>and</strong> M. Iregui………………………………………………………...<br />

890-899<br />

900-905<br />

906-916<br />

917-923<br />

924-930<br />

931-938<br />

939-950<br />

951-955<br />

956-967<br />

968-975<br />

976-988<br />

989-995<br />

996-1006<br />

XIII


Modern Research <strong>and</strong> Educational Topics <strong>in</strong> Microscopy.<br />

_______________________________________________________________________________________________<br />

©FORMATEX 2007<br />

A. Méndez-Vilas <strong>and</strong> J. Díaz (Eds.)<br />

Laurdan Generalized Polarization: from cuvette to microscope<br />

S. A. Sanchez, M.A.Tricerri, G. Gunther <strong>and</strong> E.Gratton........................................................<br />

Practical Aspects of Immunomicroscopy on Plant Material<br />

S. Marttila <strong>and</strong> K. Santén........................................................................................................<br />

Electron Microscopy <strong>in</strong> Develop<strong>in</strong>g Countries: What can be done to get these<br />

countries more <strong>in</strong>volved?<br />

V. B. Meyer-Rochow..............................................................................................................<br />

Inexpensive Digital Microscopy Workstations Engage Students <strong>in</strong> Integrative<br />

<strong>Biology</strong><br />

G. S. Withers <strong>and</strong> C. S. Wallace.............................................................................................<br />

1007-1014<br />

1015-1021<br />

1022-1027<br />

1028-1033<br />

XIV

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