AIM - Advanced Identification Methods - Experts for DNA barcoding and metabarcoding

Biodiversitymonitoring 2.0
How exactly that works
Our metabarcoding technology allows an analysis of thousands of
organisms - from hundreds of samples - simultaneously.
#5
Quality Control of
the Data
#1
Sampling
Mixed samples are
collected in the field, e.g.,
samples from Malaise
traps, soil traps, pan traps,
soil, stem eclectors,
#2
net catches, bat faeces,
bark mulch, macrozoobenthos,
processed food
or animal feed. blblblblblbl
Sample storage & shipping
The samples are collected in high
percentage ethanol and stored in
cool and dark conditions. It is also
possible to analyze dried samples,
such as faeces, bark, or soil
samples. Such samples are dried
immediately and shipped as soon
as possible. blblblblblblblblblblbllbb
#3
#4
DNA ExtraCtion
From the mixed samples,
the DNA of all organisms
in the sample is extracted.
AmplifiCation of the
Barcoding-Region
The genetic region
required for DNA
barcoding is amplified.
Then, the DNA fragments
of each sample are given
a unique marker, and are
sequenced. bbbbbbbbb
#6
In the first bioinformatics
step, the raw sequence
data is quality filtered,
cleaned, clustered, and
prepared for database
comparison.
b
Reference Database
Search
Cleaned, clustered
sequences are compared
with publicly available
databases (e.g. BOLD,
NCBI GenBank) to identify
library sequences that
closely resemble them.bb
#7
Philonthus politus
BOLD: AAP7363
customer reports
Depending on the
question, detailed species
lists are created and
supplemented with further
metadata (e.g., red
list status, pest status,
invasion potential, occurence).
In addition,
statistical evaluations and
special graphic representations
are created
according to customer
specification.
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