AIM - Advanced Identification Methods - Experts for DNA barcoding and metabarcoding

Advanced Identification Methods Gmbh
Experts for DNA barcoding and metabarcoding

WHAT WE DO
We identify species using their DNA
As leading DNA barcoding experts, we are able to not only
identify all the pests and common species, but also digitally
represent and evaluate biodiversity in its entirety!
Thus, we contribute significantly to the development of a comprehensive
online genetic database of all animal species.
DNA barcode reference databases are growing continuously and
are curated by experts, museums and state collections around
the world.
Because: we can only protect what we know!
Detection of invasive species
Determination of pest species
Identification of species compositions
Analysis of processed food

Together with you
HOW WE DO THIS
We use a highly specialized, efficient, cutting-edge DNA barcoding
workflow.
This allows us to quickly and efficiently
identify the different species - especially for
many samples at the same time. With DNA
barcode fragments, we can identify the
various species, even if taxonomic expertise
is lacking or a sample is morphologically
indeterminable (e.g., insect eggs, larvae, or
processed foods). blalalalalallalalalalalalalal
Your sample Our analysis Your report

Biodiversitymonitoring 2.0
How exactly that works
Our metabarcoding technology allows an analysis of thousands of
organisms - from hundreds of samples - simultaneously.
#5
Quality Control of
the Data
#1
Sampling
Mixed samples are
collected in the field, e.g.,
samples from Malaise
traps, soil traps, pan traps,
soil, stem eclectors,
#2
net catches, bat faeces,
bark mulch, macrozoobenthos,
processed food
or animal feed. blblblblblbl
Sample storage & shipping
The samples are collected in high
percentage ethanol and stored in
cool and dark conditions. It is also
possible to analyze dried samples,
such as faeces, bark, or soil
samples. Such samples are dried
immediately and shipped as soon
as possible. blblblblblblblblblblbllbb
#3
#4
DNA ExtraCtion
From the mixed samples,
the DNA of all organisms
in the sample is extracted.
AmplifiCation of the
Barcoding-Region
The genetic region
required for DNA
barcoding is amplified.
Then, the DNA fragments
of each sample are given
a unique marker, and are
sequenced. bbbbbbbbb
#6
In the first bioinformatics
step, the raw sequence
data is quality filtered,
cleaned, clustered, and
prepared for database
comparison.
b
Reference Database
Search
Cleaned, clustered
sequences are compared
with publicly available
databases (e.g. BOLD,
NCBI GenBank) to identify
library sequences that
closely resemble them.bb
#7
Philonthus politus
BOLD: AAP7363
customer reports
Depending on the
question, detailed species
lists are created and
supplemented with further
metadata (e.g., red
list status, pest status,
invasion potential, occurence).
In addition,
statistical evaluations and
special graphic representations
are created
according to customer
specification.
b

Protecting species and their diversity!
Why we do that
We want to help protect biodiversity and find out what causes are responsible
for the disappearance of species.
Mountains of undetermined samples
Various institutes, museums and centers have
been collecting samples from monitoring
projects for years. However, they lack the
budget and the enormous amount of time
required to process, sort and determine all
these organisms and mixed samples using
classical methods.
We can analyze, characterize and digitally archive samples
collected over decades - Now!

S tan dard one- time analysis of data
By default, samples or DNA sequences
from monitoring projects are only evaluated
or synchronized with the databases once.
The databases, however, are constantly
evolving, and the information about existing
and new species, as well as additional lists
(e.g., red list) are regularly updated. Once
generated, sequences are generally not
continuously compared with new data.
Additionally, results from old monitoring
projects are not comparable, due to, for
example, different evaluation methods or
different nomenclature. bbbbbbbbbbbbbbb
We can keep existing DNA data up-to-date, using dynamic matching, and compare
and extend those results with newly incoming information in the years to come.
Automation of routine species identification
We not only have a species extinction
crisis - we also have a taxonomic
expertise crisis! The experts,
taxonomists, with the invaluable
knowledge for species determination
and novel species identification are
dying out. bbbbbbbbbbbbbbbbbbbbbbb
So, why not relieve the few taxonomists
we have left of these routine tasks, so
that they can concentrate on identifying
new species from non-focus groups,
and use technology for monotone
species identification of thousands of
mixed samples? bbbbbbbbbbbbbbbbbb
With our technology, we support projects that identify species en masse, giving specialists time for less
routine, and more specialized taxonomic questions, as well as the identification of new species!

OUR OFFER

DNA Barcoding of Individuals:
Animals, plants, fungi, microorganisms, lichens
Organisms:
Animals, plants, fungi,
microorganisms, lichens
Sample / starting material:
Whole individuals, parts
(e.g., legs), larvae, eggs
Results:
Within 1-2 weeks
after sample receipt
Procedure: :
• Sequencing
using Sanger analysis
• Comparison of sequences
with the reference libraries of BOLD database
(www.boldsystems.org) and NCBI GenBank
(www.genbank.com)
• Unique assignment
to target organism
Specify Kit - complete solutions for the determination of individuals.
Contains sample containers with high percentage alcohol for storage and shipping of
individuals. You can insert the organisms directly and send them - we will do the rest!
Contact us at i n f o @a i m e t h o d s - l a b . c o m

DNA Me tabarcoding
Analysis of all species in a mixed sample
Organisms:
Animals, plants, fungi,
microorganisms, lichens
Procedure:
• Sequencing
using High Throughput Sequencing (HTS)
Sample / starting material:
Insect traps, soil traps or samples,
stomach contents, faecal samples,
processed foods etc.
• Comparison of sequences
with the reference libraries of BOLD
database (www.boldsystems.org) and
NCBI GenBank (www.genbank.com)
Results:
Within 2-6 weeks
after sample receipt
• Generation of customized reports
(Contains, e.g., entire species lists, Krona
graphs and statistical evaluations)
Economy Run - The cheap alternative, for ALL who can wait
a bit for their results.
You receive our best bulk discounts, even for a small amount of samples and get your
results within 6-12 weeks of sample receipt.
Contact us at i n f o @a i m e t h o d s - l a b . c o m

population genetics
Comparison of conspecifics
Organisms:
Animals (individuals of one
species, one population or
closely related species)
Sample / starting material:
Whole individuals, parts or
extracted DNA
Results:
Within 2-6 weeks
after sample receipt
Procedure:
• Sequencing of the mitochondrial genomes
using Illumina Nextera XT technology
• Comparison and mapping of the sequences
to reference genomes
NCBI GenBank (www.genbank.com)
• Creation of customized reports
(Contains, e.g., family relationships)
C ontac t us at i n f o @a i m e t h o d s - l a b . c o m

AIM – Advanced Identification Methods GmbH
Niemeyerstr. 1
04179 Leipzig
Germany
info@aimethods-lab.com
www.aimethods-lab.com
+ 49 / (0)341 33 20 31 743
+ 49 / (0)172 237 83 76
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