AIM - Advanced Identification Methods - Experts for DNA barcoding and metabarcoding

aimethods.lab13750

Advanced Identification Methods Gmbh

Experts for DNA barcoding and metabarcoding



WHAT WE DO

We identify species using their DNA

As leading DNA barcoding experts, we are able to not only

identify all the pests and common species, but also digitally

represent and evaluate biodiversity in its entirety!

Thus, we contribute significantly to the development of a comprehensive

online genetic database of all animal species.

DNA barcode reference databases are growing continuously and

are curated by experts, museums and state collections around

the world.

Because: we can only protect what we know!

Detection of invasive species

Determination of pest species

Identification of species compositions

Analysis of processed food


Together with you

HOW WE DO THIS

We use a highly specialized, efficient, cutting-edge DNA barcoding

workflow.

This allows us to quickly and efficiently

identify the different species - especially for

many samples at the same time. With DNA

barcode fragments, we can identify the

various species, even if taxonomic expertise

is lacking or a sample is morphologically

indeterminable (e.g., insect eggs, larvae, or

processed foods). blalalalalallalalalalalalalal

Your sample Our analysis Your report


Biodiversitymonitoring 2.0

How exactly that works

Our metabarcoding technology allows an analysis of thousands of

organisms - from hundreds of samples - simultaneously.

#5

Quality Control of

the Data

#1

Sampling

Mixed samples are

collected in the field, e.g.,

samples from Malaise

traps, soil traps, pan traps,

soil, stem eclectors,

#2

net catches, bat faeces,

bark mulch, macrozoobenthos,

processed food

or animal feed. blblblblblbl

Sample storage & shipping

The samples are collected in high

percentage ethanol and stored in

cool and dark conditions. It is also

possible to analyze dried samples,

such as faeces, bark, or soil

samples. Such samples are dried

immediately and shipped as soon

as possible. blblblblblblblblblblbllbb

#3

#4

DNA ExtraCtion

From the mixed samples,

the DNA of all organisms

in the sample is extracted.

AmplifiCation of the

Barcoding-Region

The genetic region

required for DNA

barcoding is amplified.

Then, the DNA fragments

of each sample are given

a unique marker, and are

sequenced. bbbbbbbbb

#6

In the first bioinformatics

step, the raw sequence

data is quality filtered,

cleaned, clustered, and

prepared for database

comparison.

b

Reference Database

Search

Cleaned, clustered

sequences are compared

with publicly available

databases (e.g. BOLD,

NCBI GenBank) to identify

library sequences that

closely resemble them.bb

#7

Philonthus politus

BOLD: AAP7363

customer reports

Depending on the

question, detailed species

lists are created and

supplemented with further

metadata (e.g., red

list status, pest status,

invasion potential, occurence).

In addition,

statistical evaluations and

special graphic representations

are created

according to customer

specification.

b


Protecting species and their diversity!

Why we do that

We want to help protect biodiversity and find out what causes are responsible

for the disappearance of species.

Mountains of undetermined samples

Various institutes, museums and centers have

been collecting samples from monitoring

projects for years. However, they lack the

budget and the enormous amount of time

required to process, sort and determine all

these organisms and mixed samples using

classical methods.

We can analyze, characterize and digitally archive samples

collected over decades - Now!


S tan dard one- time analysis of data

By default, samples or DNA sequences

from monitoring projects are only evaluated

or synchronized with the databases once.

The databases, however, are constantly

evolving, and the information about existing

and new species, as well as additional lists

(e.g., red list) are regularly updated. Once

generated, sequences are generally not

continuously compared with new data.

Additionally, results from old monitoring

projects are not comparable, due to, for

example, different evaluation methods or

different nomenclature. bbbbbbbbbbbbbbb

We can keep existing DNA data up-to-date, using dynamic matching, and compare

and extend those results with newly incoming information in the years to come.

Automation of routine species identification

We not only have a species extinction

crisis - we also have a taxonomic

expertise crisis! The experts,

taxonomists, with the invaluable

knowledge for species determination

and novel species identification are

dying out. bbbbbbbbbbbbbbbbbbbbbbb

So, why not relieve the few taxonomists

we have left of these routine tasks, so

that they can concentrate on identifying

new species from non-focus groups,

and use technology for monotone

species identification of thousands of

mixed samples? bbbbbbbbbbbbbbbbbb

With our technology, we support projects that identify species en masse, giving specialists time for less

routine, and more specialized taxonomic questions, as well as the identification of new species!


OUR OFFER


DNA Barcoding of Individuals:

Animals, plants, fungi, microorganisms, lichens

Organisms:

Animals, plants, fungi,

microorganisms, lichens

Sample / starting material:

Whole individuals, parts

(e.g., legs), larvae, eggs

Results:

Within 1-2 weeks

after sample receipt

Procedure: :

• Sequencing

using Sanger analysis

• Comparison of sequences

with the reference libraries of BOLD database

(www.boldsystems.org) and NCBI GenBank

(www.genbank.com)

• Unique assignment

to target organism

Specify Kit - complete solutions for the determination of individuals.

Contains sample containers with high percentage alcohol for storage and shipping of

individuals. You can insert the organisms directly and send them - we will do the rest!

Contact us at i n f o @a i m e t h o d s - l a b . c o m


DNA Me tabarcoding

Analysis of all species in a mixed sample

Organisms:

Animals, plants, fungi,

microorganisms, lichens

Procedure:

• Sequencing

using High Throughput Sequencing (HTS)

Sample / starting material:

Insect traps, soil traps or samples,

stomach contents, faecal samples,

processed foods etc.

• Comparison of sequences

with the reference libraries of BOLD

database (www.boldsystems.org) and

NCBI GenBank (www.genbank.com)

Results:

Within 2-6 weeks

after sample receipt

• Generation of customized reports

(Contains, e.g., entire species lists, Krona

graphs and statistical evaluations)

Economy Run - The cheap alternative, for ALL who can wait

a bit for their results.

You receive our best bulk discounts, even for a small amount of samples and get your

results within 6-12 weeks of sample receipt.

Contact us at i n f o @a i m e t h o d s - l a b . c o m


population genetics

Comparison of conspecifics

Organisms:

Animals (individuals of one

species, one population or

closely related species)

Sample / starting material:

Whole individuals, parts or

extracted DNA

Results:

Within 2-6 weeks

after sample receipt

Procedure:

• Sequencing of the mitochondrial genomes

using Illumina Nextera XT technology

• Comparison and mapping of the sequences

to reference genomes

NCBI GenBank (www.genbank.com)

• Creation of customized reports

(Contains, e.g., family relationships)

C ontac t us at i n f o @a i m e t h o d s - l a b . c o m


AIM – Advanced Identification Methods GmbH

Niemeyerstr. 1

04179 Leipzig

Germany

info@aimethods-lab.com

www.aimethods-lab.com

+ 49 / (0)341 33 20 31 743

+ 49 / (0)172 237 83 76

This booklet was printed on 100%

aus recycled waste paper from the

German ecolabel Blauer Engel.

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