Procs III 2011.indb - Journal of Bone & Joint Surgery, British Volume ...

362 HELLENIC ASSOCIATION OF ORTHOPAEDIC SURGERY AND TRAUMATOLOGY
anterogradely labelled with MiniRuby, a 10 kD biotinylated
dextran amine.
After 4 days the co-cultures were propagated, and
axonal sprouting occurred. The group of co-cultures
treated with NT-3 showed an improved cortical cytoarchitecture,
and sprouting axons were more frequently
observed. In NT-3-treated co-cultures where spinal cord
gray matter was directly opposed to cortical slices sprouting
axons entered the adjacent spinal cord tissue. This
phenomenon was not observed if spinal white matter was
opposed to the cortical slices, or if NT-3 was absent.
Our data suggest that the absence of repellent factors
such as white matter and the presence of neurotrophic
factors promote axonal sprouting. Co-cultures
of motorcortex and spinal cord slices combined with
anterograde axonal labelling could provide a valuable
in vitro model for the simplifi ed screening of factors
infl uencing corticospinal tract regeneration
012 THE ROLE OF TSF (TAYLOR SPATIAL
FRAME) IN TREATING TRAUMA AND BONE
DEFORMITIES IN CHILDREN
H. Tsibidakis, V. I. Sakellariou, V.
Tsouparopoulos, G. Mazis, K. Staratzis, A.
Kanellopoulos
1st Orthopaedic Department, University of
Athens, ATTIKON University General Hospital,
Greece
To study the use of TSF system in treating trauma and
bone deformities in children. To determine the diffi culties
of this process and the risk factors that lead to complications.
From January 2004, in 61 children (37 male and 24
female), 67 extremities, with a mean age 8.9 years children
a TSF external fi xator was applied for the treatment
of trauma or bone deformities. 21 children were operated
for angular deformity, 19 for bone lengthening, 10 for
rotational deformity, 6 for combined angular deformity
and lengthening and 11 for pseudoarthrosis. Intra and
postoperative diffi culties were classifi ed using the Palay
method in problems, obstacles and complications.
The rate of diffi culties was 22.2 %. Problems were
presented in 5.9% (4/67) consisting of 2 non-axial
deformities, 1 pin fracture and 1 subluxation of the
knee. Obstacles were presented in 10.4% (9/67) including
3 cases with delayed bone healing that needed infusion
DBM, 1 peroneal nerve palsy due to hematoma
formation treated with decompression of the region, 1
early bone fusion that needed re-operation and 2 cases
of percutaneous achilles lengthening. Complications
presented in 5.9% of (4/67) the cases including 1 fracture,
1 pseudoarthrosis, 1 peroneal nerve palsy and 1
limitation of range of motion in the knee (0-45 0).
The problems, obstacles and complications that presented
during treatment infl uenced the fi nal therapeutic
objective. Initial deformity, preoperative planning and
surgeon’s experience are associated with reducing the
rate of all diffi culties
013 BONE MORPHOGENETIC PROTEINS
(BMPS) EXPRESSION IN THE FEMORAL
HEADS OF PATIENTS WITH AVASCULAR
NECROSIS
1 S. Samara; 2 Z. Dailiana; 2,3 K.N. Malizos; 2 S.
Varitimidis; 1 C. Chassanidis; 4 T. Koromila; 4 P.
Kollia
1 Laboratory of Medical Genetics and
Cytogenetics, University of Thessalia, Larissa;
2 Department of Orthopaedic Surgery, University
of Thessalia, Larissa; 3 Institute of Biomedical
Research and Technology, Larissa; 4 Laboratory of
Human Genetics, School of Biology, University of
Athens, Athens
Avascular necrosis (AVN) is a disorder leading to femoral
head (FH) destruction, while BMPs are known for
their osteogenic ability. In this study we analyzed BMP-
2, BMP-4, BMP-6 and BMP-7 expression at the RNA
and protein level in the normal and necrotic sites of
the FHs.
Quantitative RT-PCR for BMP-2,-4,-6,-7 genes was
performed in samples from the normal and necrotic sites
of 52 FHs with AVN. Protein levels of BMP-2,-4,-6 were
estimated by Western Blot analysis. Statistical analysis
was performed using the t-test (p