How-to-Choose-the-Right-Immunoprecipitation-Product-for-Your-Experiment

How to Choose the Right
Immunoprecipitation Product
for Your Experiment
CD
Magnetic Beads vs. Agarose Resin
Magnetic beads
If the sample size is < 2 mL, magnetic
beads are best for manual and
automated standard IP, Co-IP, ChIP,
Capacity
Yield
Reproducibility
ChIP-Seq, RIP, and pull-down reactions
for immediate assay analysis.
Agarose resin
Magnetic
Beads
Purity-Specificity
Ease of Use
Agarose
Resin
If the sample size is > 2 mL and the
goal is aiming to purify a sufficient
Speed
amount of target protein for multiple
downstream assays, agarose is more
Multiple Samples
appropriate for column affinity
Automation
chromatography or individual large
scale spin cup IP reactions.
Tel: 1-631-624-4882
Email: info@cd-bioparticles.com

www.cdparticles.com
Starting with:
Absolute Mag Protein A
or
Absolute Mag Protein G
1 2
3
Absolute Mag Beads
Protein A or G
Antibody
Target Protein
4
Nonspecific Protein
IP with Magnetic Beads
1. Binding of the antibody to the beads
2. Add sample to the beads
3. Wash the protein-bead complex
4. Elute the protein
Advantages of Magnetic Beads for IP
Magnetic beads are a faster, simpler, and more efficient method than the use of
traditional agarose or other agarose resins for target protein pull-down, and have
become the gold standard for IP and pull-down analysis. Absolute Mag magnetic
beads help you achieve the best balance between high yield, high reproducibility and
low non-specific binding, low cost.
Tel: 1-631-624-4882
Email: info@cd-bioparticles.com

www.cdparticles.com
Capacity and yield
Magnetic beads are nonporous with a
smooth outer surface. Although they
are much smaller than agarose beads,
they have a lower theoretical binding
Highly
Sensitive
Fast
&
Easy
Flexible
capacity than agarose. But, when
individual antibodies are immobilized
on sponge-like agarose, these
Highly
Reproducible
Automation of
IP Protocol
antibodies are not always accessible to
the target proteins in a sample, which
results in the antibody lost during the
washing steps. On the contrary, they
are all accessible to an antigen if
antibodies are bound to the surface of
magnetic beads, and there is little
chance for antibodies to be lost during
Low
Background
Benefits for
Absolute Mag Technology
Reproducibility and purity
Antibody
Savings
the gentle washing steps.
When using magnetic beads and a
magnet, all the beads are held firmly
Ease of use, speed, and automation
An individual magnetic bead IP
experiment can be completed within 30
min and can process multiple samples
easily and rapidly. In addition, small
benchtop instrumentation is available
for the automation of the whole IP
protocols using magnetic beads.
to the side of the tube, and thus all
buffers are able to be removed
without touching the bead-pellet.
Moreover, magnetic beads are
uniform in size and all interactions
occur on their outer smooth surface,
so they can usually provide higher
reproducibility and purity than
agarose. Pre-clearing is generally not
necessary with magnetic beads.
Tel: 1-631-624-4882
Email: info@cd-bioparticles.com

www.cdparticles.com
Choose the Right Immunoprecipitation Product for Your Experiment
Antibody-binding IP products
If you have an antibody that recognizes your target protein (or your downstream
assay is mass spectrometry, or you don’t want the antibody co-eluted with your target
protein), you can choose antibody-binding products.
Protein A, G
Secondary Antibodies
Surface-activated Beads
Binding properties
Non-covalent
Non-covalent
Covalent
Antibody co-eluted
off the beads
Yes
Yes
No
Different ligands bind
Type of ligand
different species and
antibody subclasses
IgGs
All antibodies
with different
specificity
Mass spec
compatible
Not validated
No
Yes
Nonspecific
binding
Low
Low
Ultra-low
Products
Absolute Mag
Protein A
Absolute Mag
Protein G
Absolute Mag
Anti-Goat IgG
Absolute Mag
Anti-Rabbit IgG
Absolute Mag
Anti-Mouse IgG
Absolute Mag Amine
Absolute Mag Carboxyl
Absolute Mag Epoxy
Absolute Mag Tosyl
more
Absolute Mag
Anti-Rat IgG
Absolute Mag
Anti-Human IgG
Tel: 1-631-624-4882
Email: info@cd-bioparticles.com

www.cdparticles.com
Biotin-binding IP products
If you have a sample rich in
soluble IgGs
If you have a recombinant
antibody lacking Fc regions
If you already have streptavidin
coated magnetic beads in the
Absolute Mag
Streptavidin
Magnetic Particles
If you need a bead compatible
with mass spectrometry
Recombinant protein (fusion tag) IP products
Absolute Mag Ni-NTA
If you have a
recombinant
(fusion tagged)
protein
Absolute Mag Glutathione
Absolute Mag Anti-HA
Absolute Mag Ni-IDA
For more information,
view our website: www.cd-bioparticles.com
Tel: 1-631-624-4882
Fax: 1-631-938-8221
Email: info@cd-bioparticles.com
Address: 45-1 Ramsey Road, Shirley, NY 11967, USA 5