Medical Cannabis Report 2023 update

Revolutionizing the production

of medical cannabis: the

impact of ecological water

regeneration on the growth

and quality of crops in

greenhouses.

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P r e p a r e d B y :

T o m e r S a y a g

B . S . E n g i n e e r

M e d i c a l c a n n a b i s c o n s u l t a n t

R E V O L U T I O N I Z I N G T H E P R O D U C T I O N O F M E D I C A L C A N N A B I S - R E P O R T

Abstract

Introduction

Methods

Results

Discussion

Conclussion

References

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INDEX

T O M E R S A Y A G

H I G H - P E R F O R M A N C E M E D I C A L C A N N A B I S C O N S U L T A N T

V O R T E X C A N N A B I S T E C H @ G M A I L . C O M + 9 7 2 5 0 - 4 5 9 - 5 8 5 7

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Effect of an ecological water regeneration

system on the efficiency, growth, and quality of

medical cannabis greenhouse crops

Abstract:

Using an ecological water regenerator (RNX NERO POWER® by

Euronix Sistemas, S.L, Valencia-Spain) in the cultivation of

medical cannabis in a greenhouse significantly improves plant

growth and quality, reduces water and fertilizer use, and

increases energy efficiency. In our study, plants irrigated with

the ecological water regenerator device had a significantly

higher average height, faster growth, higher THC levels, and

greater flower dry biomass than the control group. We

recommend using the RNX NERO POWER® system in medical

cannabis greenhouse cultivation for higher quality and

performance.

T O M E R S A Y A G



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Introduction:

Medical cannabis has become a popular alternative to

traditional medications for the treatment of various medical

conditions. It has been shown to have numerous therapeutic

benefits, including pain relief, reduction of inflammation, and

improvement of sleep quality (Ebbert JO et al., 2018). As a

result, the cultivation of medical cannabis has become an

important industry, with greenhouse cultivation being one of

the most common methods for producing high-quality plants

(Sorrentino G, 2021).

However, medical cannabis greenhouse cultivation also has its

own set of challenges, including the need to optimize water

and nutrient management to ensure optimal plant growth

(Eichhorn et al., 2019).

Greenhouse cannabis crops use a variety of water sources for

irrigation. Unfortunately, the quality of water is often

overlooked as a potential source of plant growth. Water

quality is most important for crops in a greenhouse because

irrigation is the only source of water.

T O M E R S A Y A G



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One way to improve water and nutrient management in

greenhouse cultivation is by using an ecological water

regenerator. These devices work by utilizing various natural

phenomena, such as bioelectric phenomena of mineral origin,

suction vortex movements, hydrodynamic cavitation,

geometric aspects, and resonance, to improve the quality of

water. Specifically, they have been shown to reduce the

surface tension of water and increase its oxygen content,

which can have positive effects on plant growth and

development (Brendel O., 2021; Lamm et al., 2014). Using an

ecological water regenerator can help ensure that greenhouse

plants receive the optimal amount of water and nutrient

leading to healthier and more productive crops.

In this study, we evaluated the effect of using an ecological

water regenerator (RNX NERO POWER®) on the cultivation of

medical cannabis in a greenhouse.

T O M E R S A Y A G



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Methods

Experimental design:

To evaluate the effect of using RNX NERO POWER® on the

cultivation of medical cannabis in a greenhouse setting, we

conducted a 4-month experiment at a leading farm in the

Israeli market (Evergreen Solomon Sgula). A total of 420

cannabis plants were randomly assigned to the experimental

group (n=280) and the control group (n=140). The water used

in the greenhouse was sourced from the drinking water

system, filtered through a carbon filter, mixed with fertilizer,

and distributed in different irrigation lines. In the experimental

group, the water line was treated with RNX NERO POWER®

before being used to irrigate the plants (n=280). The water line

in the control group was used directly without treatment with

the water regenerator (Figure 1).

Figure 1: In the experimental group (n=280), the water line was previously

regenerated by RNX NERO POWER®. In the control group (n=140) the

water was utilized directly without treatment with the water regenerator.

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T O M E R S A Y A G




Variables quantified in the different cultivation stages:

We quantified several variables at different stages of

cultivation, including the vegetative stage (until week 6-7),

flowering stage (until week 16), and harvest stage. At the end

of the vegetative stage, we evaluated height (cm), growth rate

(cm/day), and height over control (%). At the end of the

followering stage, the flowers were harvested and placed in a

drying chamber. Then the biomass of dry weight (kg) and THC

level (%) was determined. We also calculated the total amount

of water and fertilizer saved before the harvest. The water

saving was calculated based on the shortening of the growth

cycle. After the harvest, we determined the wet root biomass.

Statistical analyses were performed using IBM SPSS Statistics

for Windows, Version 26.0. The data were analyzed using the

Kruskal-Wallis test and the results were considered

statistically significant at a p-value < 0.05 (Figure 2).

Figure 2: Data cleaning and analysis data process using the statistical

package IBM SPSS Statistics for Windows, Version 26.0. Armonk, NY:

IBM Corp.

T O M E R S A Y A G



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Results:

The results of our experiment showed that the use of RNX

NERO POWER® had a significant effect on the cultivation of

medical cannabis in a greenhouse setting. After six weeks at

the end of the vegetative stage, the average height of the

plants in the experimental group (94.48 ± 0.817 cm) was

significantly higher than that of the plants in the control group

(74.29 ± 0.92 cm). This represents a 27.2% increase in height

for the experimental group compared to the control group

(Figures 3 y 4).

Figure 3: Average height of the plants in the last weeks of the

vegetative stage in the experimental (RNX NERO POWER®) and

control group (bulk water). * Significative difference concerning

the control group. Kruskal Wallis test.

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Figure 4: Average height over control ratio of the plants in the

experimental (RNX NERO POWER®) and control group (bulk

water).

The experimental group also had a significantly higher

average growth rate (2.39 cm/day) compared to the control

group (1.85 cm/day) (Figure 5). This resulted in a shortened

growth cycle of 3-4 weeks, with the vegetative target height

being reached in just 3 weeks (compared to the usual 6-7

weeks).

In addition, the total biomass of dry flowers was 17% higher in

the experimental group compared to the control group (Figure

6). The THC levels in the experimental group were 5.7% higher

than those in the control group (data not shown).

The total average wet biomass of the root system was also

significantly higher in the experimental group (50.8 grams)

compared to the control group (39.2 grams) (Figure 7).

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Figure 5: Higher average growth rate of the plants in the


water). * Significative difference concerning the control group.

Kruskal Wallis test.

Figure 6: Total biomass of dry flowers of the plants in the


water). * Significative difference concerning the control group.

Kruskal Wallis test.

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T O M E R S A Y A G




Furthermore, the use of RNX NERO POWER® resulted in a

significant reduction in the total water and fertilizer usage

for the cultivation of medical cannabis in the greenhouse.

The experimental group showed a saving of approximately

8.4-14 liters of fertilizer and water per plant due to the

shortened growth cycle, compared to the control group.

(data not shown).

Figure 7: Images of the root systems from the experimental and control

groups were taken randomly. The average weight of the wet biomass of the

root systems was significantly higher in the experimental group (50,8 g)

compared to the control group (39,2 g)

Discussion:

The results of this study demonstrate the positive impact of

the RNX NERO POWER® ecological water regenerator on

the cultivation of medical cannabis in a greenhouse. The

study included two groups of plants: one group that was

irrigated with the regenerator device, and a control group

that was not.

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T O M E R S A Y A G




The results showed that during the vegetative stage, the

plants in the experimental group had a significantly higher

average height, increased height ratio compared to the

control group, and a higher growth rate. These findings

confirm the beneficial effect of using the ecological water

regenerator RNX NERO POWER® on the growth of medical

cannabis.

Not only did the plants in the experimental group grow

significantly faster than those in the control group, they also

had significantly higher flower dry biomass and THC levels.

Additionally, the use of water and fertilizers was reduced in

the experimental group compared to the control group.

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T O M E R S A Y A G




The higher THC levels observed in the plants of the

experimental groups may be the result of the improved water

quality provided by RNX NERO POWER®. Research has shown

that THC synthesis is influenced by various factors, including

water and nutrient availability (Trancoso, I., 2022). It is

possible that the enhanced growth and health of the plants in

the experimental groups may have been facilitated by the

improved water quality.

The results of the study indicate that the use of RNX NERO

POWER® resulted in a shorter growth cycle, with plants in the

experimental group reaching the appropriate size and physical

characteristics for flowering at an earlier stage than the

control group.

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The greenhouse usually starts the flowering stage when the

plants reach 35-40 cm. Specifically, the experimental group

reached a target height of 35 cm at 3 weeks, while the control

group did not reach this height until 4 weeks. Furthermore, the

plants in the control group were not as well developed in

terms of leaf and branch growth, suggesting that they were

not yet ready to enter the flowering phase. As a result, the

control group was only able to enter the flowering phase 3-4

weeks later than the experimental group.

With this innovative ecological water regeneration system, we

are sustainably meeting new standards of quality and

performance in medical cannabis cultivation. Based on these

results, we recommend the use of the RNX NERO POWER®

ecological water regenerator when growing medical cannabis

in greenhouses, as it can significantly increase energy

efficiency and improve plant growth and quality.

T O M E R S A Y A G



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These results demonstrate that RNX NERO POWER® ecological

water regenerator not only promotes the growth of medical

cannabis, but also improves its quality and reduces resource

consumption.

Conclusion:

The use of RNX NERO POWER® ecological water regenerator

in the cultivation of medical cannabis in a greenhouse has

been shown to significantly improve the growth and quality of

the plants. Plants irrigated with this system had higher growth,

more flower biomass, and increased THC levels. Additionally,

the use of water and fertilizers could be reduced, resulting in a

more efficient and sustainable growth cycle. These results

demonstrate the potential of this innovative system to achieve

new standards of quality and performance in the cultivation of

medical cannabis in a sustainable way. Based on these

findings, we recommend the use of the RNX NERO POWER®

ecological water regenerator in the cultivation of medical

cannabis in a greenhouse.

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T O M E R S A Y A G




References:

Brendel O. The relationship between plant growth and water

consumption: a history from the classical four elements to

modern stable isotopes. Annals of Forest Science (2021) 78: 47.

Ebbert JO, Scharf EL, Hurt RT. Medical Cannabis. Mayo Clin

Proc. 2018 Dec;93(12):1842-1847.

Eichhorn Bilodeau S, Wu BS, Rufyikiri AS, MacPherson S,

Lefsrud M. An Update on Plant Photobiology and Implications

for Cannabis Production. Front Plant Sci. 2019 Mar 29;10:296.

Kumar, V., & Singh, A. (2018). Advantages and challenges of

greenhouse vegetable production. In Advances in Vegetable

Production (pp. 1-15). Springer, Singapore.

Lamm, M. G., Strand, A. E., & Hermansen, J. E. (2014). Root

growth and water uptake of plants grown in water with

different surface tension and oxygen content. Plant and Soil,

379(1-2), 105-118.

Sorrentino G. Introduction to emerging industrial applications

of cannabis (Cannabis sativa L.). Rend Lincei Sci Fis Nat.

2021;32(2):233-243.

Trancoso, I.; de Souza, G.A.R.; dos Santos, P.R.; dos Santos,

K.D.; de Miranda, R.M.d.S.N.; da Silva, A.L.P.M.; Santos, D.Z.;

García-Tejero, I.F.; Campostrini, E. Cannabis sativa L.: Crop

Management and Abiotic Factors That Affect

Phytocannabinoid Production. Agronomy 2022, 12, 1492.

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As a medical cannabis production consultant, I am always

looking for ways to improve the sustainability and efficiency

of cannabis growing operations. That's why I'm excited to

introduce the RNX NERO POWER® ecological water

regeneration system.

If you are a medical cannabis production consultant or a

grower looking for ways to improve your sustainability and

efficiency, I highly recommend you to consider the RNX NERO

POWER® eco-friendly water regeneration system. This

innovative technology has the potential to revolutionize the

way we grow cannabis and is an important step towards a

more sustainable and profitable industry.

Do you want to increase the sustainability and efficiency of

your medical cannabis cultivation with RNX NERO POWER®?

Contact me for more information.

T o m e r S a y a g

B . S . E n g i n e e r

T e c h n i o n - I s r a e l I n s t i t u t e o f T e c h n o l o g y

M e d i c a l c a n n a b i s c o n s u l t a n t

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Medical Cannabis Report 2023 update

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