Mikrobiologische Reinigung und Desinfektion Wirksamkeit einer dreistufigen Ultraschallbearbeitung Protokoll für CAD-CAM-Implantataufbauten

The Journal of Advanced Prosthodontics
https://doi.org/10.4047/jap.2022.14.5.273
sions were obtained:
A three-step ultrasonic cleaning and disinfection
process is reproducible and complies with the standardized
reprocessing and disinfection specifications
for custom one- or two-piece CAD-CAM implant
abutments made of titanium or ceramic. The cleaning
solution with the active ingredients chlorhexidine
gluconate and cetrimide, followed by ethyl alcohol
and medically pure water in the ultrasonic treatment,
leads to effective bacterial decontamination. Ultrasonic
reprocessing resulted in a substantial decrease
of residual protein of less than 80 µg and a reduction
in microbiota of more than 7 lg levels of colony-forming
units for all abutment types, exceeding the effect
required for disinfection.
ACKNOWLEDGEMENTS
The authors gratefully acknowledge Jochen Schlenker
for his support and valuable advise as well as Patrick
Mühlbeyer for his excellent technical assistance.
The authors thank BEGO Medical GmbH for providing
the test samples for the experimental investigation.
REFERENCES
1. Kapos T, Evans C. CAD/CAM technology for implant
abutments, crowns, and superstructures. Int J Oral
Maxillofac Implants 2014;29:117-36.
2. Zarauz C, Pitta J, Pjetursson B, Zwahlen M, Pradies G,
Sailer I. Esthetic outcomes of implant-supported single
crowns related to abutment type and material: a
systematic review. Int J Prosthodont 2021;34:229-49.
3. Gehrke P, Bleuel K, Fischer C, Sader R. Influence of
margin location and luting material on the amount of
undetected cement excess on CAD/CAM implant abutments
and cement-retained zirconia crowns: an in-vitro
study. BMC Oral Health 2019;19:111.
4. Ivanovski S, Lee R. Comparison of peri-implant and
periodontal marginal soft tissues in health and disease.
Periodontol 2000 2018;76:116-30.
5. Rupp F, Gittens RA, Scheideler L, Marmur A, Boyan BD,
Schwartz Z, Geis-Gerstorfer J. A review on the wettability
of dental implant surfaces I: theoretical and experimental
aspects. Acta Biomater 2014;10:2894-906.
6. Canullo L, Tallarico M, Radovanovic S, Delibasic B,
Covani U, Rakic M. Distinguishing predictive profiles
for patient-based risk assessment and diagnostics
of plaque induced, surgically and prosthetically triggered
peri-implantitis. Clin Oral Implants Res 2016;27:
1243-50.
7. Welander M, Abrahamsson I, Berglundh T. The mucosal
barrier at implant abutments of different materials.
Clin Oral Implants Res 2008;19:635-41.
8. Canullo L, Micarelli C, Lembo-Fazio L, Iannello G,
Clementini M. Microscopical and microbiologic characterization
of customized titanium abutments after
different cleaning procedures. Clin Oral Implants Res
2014;25:328-36.
9. Gehrke P, Tabellion A, Fischer C. Microscopical and
chemical surface characterization of CAD/CAM zircona
abutments after different cleaning procedures. A
qualitative analysis. J Adv Prosthodont 2015;7:151-9.
10. Gehrke P, Abazari C, Schlichter K, Fischer C, Duddeck
D, Romanos GE, Weigl P. Qualitative and semi-quantitative
assessment of processing-related surface contamination
of one- and two-piece CAD/CAM abutments
before and after ultrasonic cleaning. Materials
(Basel) 2020;13:3225.
11. Canullo L, Micarelli C, Iannello G. Microscopical and
chemical surface characterization of the gingival portion
and connection of an internal hexagon abutment
before and after different technical stages of preparation.
Clin Oral Implants Res 2013;24:606-11.
12. Mishra PK, Wu W, Rozo C, Hallab NJ, Benevenia J,
Gause WC. Micrometer-sized titanium particles can induce
potent Th2-type responses through TLR4-independent
pathways. J Immunol 2011;187:6491-8.
13. Infection control recommendations for the dental office
and the dental laboratory. ADA Council on Scientific
Affairs and ADA Council on Dental Practice. J Am
Dent Assoc 1996;127:672-80.
14. ISO 17664-1. Processing of health care products - Information
to be provided by the medical device manufacturer
for the processing of medical devices - Part
1: Critical and semi-critical medical devices. International
Standard Organization (ISO); Geneva; Switzerland,
2021.
15. Park JH, Olivares-Navarrete R, Baier RE, Meyer AE,
Tannenbaum R, Boyan BD, Schwartz Z. Effect of
cleaning and sterilization on titanium implant surface
properties and cellular response. Acta Biomater 2012;
282 https://jap.or.kr