1 Running head: Steryl glycoside mutants Name: Seth DeBolt ...
1 Running head: Steryl glycoside mutants Name: Seth DeBolt ...
1 Running head: Steryl glycoside mutants Name: Seth DeBolt ...
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A<br />
FS + SE (mg/gram dry)<br />
B<br />
SG + ASG (mg/gram dry)<br />
6<br />
5<br />
4<br />
3<br />
2<br />
1<br />
0<br />
0.4<br />
0.35<br />
0.3<br />
0.25<br />
0.2<br />
0.15<br />
0.1<br />
0.05<br />
0<br />
wild type<br />
wild type<br />
wild type<br />
wild type<br />
Wild type ugt80A2,B1 Wild type ugt80A2,B1 Wild type ugt80A2,B1<br />
Leaf<br />
ugt80A2,B1<br />
Leaf Stem Inflorescence<br />
ugt80A2,B1<br />
wild type<br />
ugt80A2,B1<br />
ugt80A2,B1<br />
wild type<br />
ugt80A2,B1<br />
ugt80A2,B1<br />
Wild type ugt80A2,B1 Wild type ugt80A2,B1 Wild type ugt80A2,B1<br />
Leaf<br />
Stem<br />
Stem<br />
In�or/Sili<br />
In�or/Sili<br />
Leaf Stem Inflorescence<br />
Fig. 1. Analysis of sterols and sterol derivates in ugt80A2,B1 mutant relative to wild-type.<br />
Total sterol derivatives were quanti�ed and compared between wild-type and mutant<br />
plants in mg per gram dry weight-1 as A) FS + SE and B) SG + ASG. FS+SE was the sum of<br />
FS measured by GC-FID + sterols measured by GC-FID released from SE fraction after<br />
saponi�cation. Values are the mean of three replicates and experimental analysis was<br />
duplicated; error bars indicate standard error from the mean.
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