REPRODUCTION - Facultad de Ciencias Veterinarias
REPRODUCTION - Facultad de Ciencias Veterinarias
REPRODUCTION - Facultad de Ciencias Veterinarias
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Composition Skim Milk diluter<br />
Solution A (500 mL)<br />
852<br />
<strong>REPRODUCTION</strong><br />
Skim milk………………………………………………………………………..55g<br />
Note: maximum heated to 80 ° C to not <strong>de</strong>nature the protein contend<br />
Final Solution<br />
Skim milk…………………………………………………………………....73.0 mL<br />
Egg yolk……………………………………………………………………..20.0 mL<br />
Glycerol……………………………………………………………………….7.0 mL<br />
Streptomycin sulphate………………………………………………………..700 mg<br />
Penicillin-G-potassium………………………………………………………...70 mg<br />
Osmolarity to 290 ± 5mOsm<br />
pH 6.8 to 7.0<br />
Composition of CEBRAN-1 diluter (Coconut water)*<br />
Solution A - 125 mL (distillate or <strong>de</strong>ionised water)<br />
Coconut water……………………………………………………………50 mL<br />
Sodium citrate 5%..................................................................................... 25 mL<br />
*Coconut fruit must be green, not ripe<br />
Solution B – 125 mL (distillate or <strong>de</strong>ionised water)<br />
Solution A……………………………………………………………….. 90.0 mL<br />
Egg yolk………………………………………………………………… 10.0 mL<br />
Streptomycin sulphate……………………………………………………..700mg<br />
Penicillin-G-potassium…………………………………………………….70 mg<br />
Final solution<br />
Solution B…………………………………………………………………93.0 mL<br />
Glycerol…………………………………………………………………….7.0 mL<br />
Osmolarity to 290 ± 5mOsm<br />
pH 6.8 to 7.0<br />
Proceedings 9 th World Buffalo Congress
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