T7 RNA Polymerase Expression System for Bacillus megaterium
T7 RNA Polymerase Expression System for Bacillus megaterium
T7 RNA Polymerase Expression System for Bacillus megaterium
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© MoBiTec GmbH, 2012 Page 14<br />
Fig.6 Incomplete sequence of pP<strong>T7</strong>. <strong>T7</strong> <strong>RNA</strong>P promoter and terminator are underlined.<br />
The multiple cloning site (MCS) is marked with a red bar. The last unique restriction site<br />
upstream of the <strong>T7</strong> <strong>RNA</strong>P terminator is marked with a red rectangle. The stop codon<br />
(TAA) is illustrated by a dashed line.<br />
For complete sequences please check www.mobitec.com<br />
Fig. 5 Map of pP<strong>T7</strong>-SPlipA.<br />
Replicon derived from B. cereus<br />
(repU); <strong>T7</strong> <strong>RNA</strong> polymerase<br />
promoter and terminator;<br />
multiple cloning site (MCS);<br />
signal peptide LipA (splipA);<br />
resistance genes against<br />
ampicillin (Amp R ) and<br />
tetracycline (Tet R ).<br />
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