Analysis of Impurities in Curcumin - Shimadzu

No.C59
■ PDA Data
Fig.3 shows the UV spectra of curcuminoids. All of
them clearly possess a maximum absorption
wavelength near 420 nm. When the peak purity is
mAU
17.5
(1)
15.0
12.5
10.0
7.5
5.0
2.5
200
246
Fig.3 UV Spectra of Curcuminoids (1: Bisdemethoxycurcumin, 2: Demethoxycurcumin, 3: Curcumin)
Fig.4 Purity Curves of Curcuminoids (1: Bisdemethoxycurcumin, 2: Demethoxycurcumin, 3: Curcumin)
■ Another Impurity
Focusing on the peak (compound X) at 5.35 minutes
in the mass chromatograms of fig.4 (2), the UV
spectrum and mass spectrum were obtained for this
peak, as shown in the fig.5. The maximum absorption
wavelength is in the vicinity of 370 nm, and the m/z
417
mAU mAU
150
125
(2) 421
1000
900
800
(3)
600
0.0 0 0
Fig.5 Mass chromatograms of curcumin standard solution (grade A, (a)) and UV spectrum (b), mass spectrum (c) of compound X
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100
75
50
25
240
calculated from the grade A data, an impurity was
detected at 5.35 minutes (fig.4 (2)).
300 400 500 nm 200 300 400 500 nm 200 300 400 500 nm
0.85
0.80
0.75
0.70
0.65
0.60
Purity Curves
Zero Line
mAU
peak 15.0
(1) 14.0
13.0
12.0
11.0
Purity Curves
Zero Line
0.6
0.5
0.4
mAU
peak
130
(2)
120
110
100
0.9
Purity Curves
Zero Line
0.8
0.7
mAU
peak 900
(3)
800
700
0.55
10.0
90 0.6
600
0.50
0.45
0.40
9.0
8.0
0.3
0.2
80
70
0.5
500
0.35
7.0
60
0.4
400
0.30
0.25
0.20
6.0
5.0
4.0
0.1
0.0
50
40
0.3
0.2
300
0.15
0.10
0.05
3.0
2.0
-0.1
30
20 0.1
200
100
0.00
1.0 -0.2
10
0.0
0.0 0
0
4.2 4.3 4.4 4.5 min 5.00 5.25
min
5.75 6.00 6.25 6.50 min
Inten. (× 1,000,000)
3.50
3.25 (a)
3.00
2.75
2.50
2.25
2.00
1.75
1.50
1.25
1.00
0.75
0.50
0.25
0.00
0.0
■Peaks
1. Bisdemethoxycurcumin,
2. Demethoxycurcumin,
3. Curcumin
1
2
X
3
TIC
m/z307
m/z337
m/z369
m/z367
1.0 2.0 3.0 4.0 5.0 6.0 7.0 min
NOTES:
✽This Application News has been produced and edited using information that was available when the
data was acquired for each article. This Application News is subject to revision without prior notice.
700
600
500
400
300
200
100
263
426
369 negative ion was detected. Therefore, the
molecular weight of compound X is presumed to 370.
Thus, by using both PDA and MS in UFLC detection,
greater efficiency is believed to be possible.
10.0
(× 1,000,000)
(b)
7.5
5.0
373
2.5
0.0
255
282
200 300 400 500 nm
Inten. (× 10,000)
6.0
5.0
4.0
3.0
2.0
(c)
369
1.0
169
397
0.0
200 300 400 m/z
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