Depletion of eosinophils in mice through the use - Journal of ...

phils. However, the surface expression of CCR3 on mouse Th2
cells, if present, was too low for detection by flow cytometry. In
addition, our study shows that mouse Th2 cells are not
responsive to eotaxin either by calcium mobilization assay or
chemotaxis. This contrasts with a recent report of preferential
staining with an anti-huCCR3 mAb as well as the eotaxinmediated
chemotaxis and calcium flux of human Th2 cells [8].
Our observation of low levels of mCCR3 mRNA in activated
Th2 cells, however, is consistent with a more recent report on
CCR3 mRNA expression observed in human Th1 and Th2 cells
[27]. It is quite possible that CCR3 expression on Th2
population is tightly linked to an as yet unknown activation
state and this could explain the differences observed between
different reports. We have also demonstrated that anti-mCCR3
mAbs of the rat IgG2b isotype can also be used to achieve
partial to complete depletion of eosinophils in vivo. Treated
animals were used in restimulation assays of lung cells, which
showed no increase in Th2-induced cytokine levels. In short,
these assays taken together show that CCR3 is not a significant
chemokine receptor for mouse Th2 cells. Recent studies have
shown that other chemokine receptors such as CCR8 may have
a far more significant role on Th2-specific cellular migration
[28]. These antibodies should be useful in future studies aimed
at determining the roles of eosinophils in infectious and
inflammatory diseases.
ACKNOWLEDGMENTS
DNAX Research Institute is supported by Schering-Plough
Corporation. The authors would like to thank Debra Liggett for
synthesis of DNA, Dan Gorman, Allison Helms, and Connie
Huffine for DNA sequencing analysis, Dr. Terri McClanahan
and Karin Bacon for providing cDNA libraries, Dr. Donna
Rennick for histology consultation, Dr. Joe Hedrick for chemotaxis
analysis, Dr. Kenneth Soo for his helpful suggestions, and
Gary Burget and Dr. Maribel Andonian for graphics work.
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