Oral Presentations - Federation of Clinical Immunology Societies

S30 Abstracts
production level of IL-6 has been correlated with allele
inherited in individuals. Therefore, the aim of current study
was to assess whether IL-6 (−174) polymorphism was
associated with susceptibility to brucellosis. Method: One
hundred and seventy-five patients with brucellosis and 77
healthy animal husbandmen who had infected animals and
consumed their dairy products, joined this study. DNAs
extracted from samples were genotyped for IL-6 (−174 G/C)
polymorphism using AS-PCR method. Results: Our results
showed that IL-6 (−174) gene polymorphism was distributed
similarly in the patient and control groups (P =0.3).
Discussion: Although we found no association between IL-6
(−174) gene polymorphism and susceptibility to brucellosis
we cannot exclude that other polymorphisms within this
gene or its receptor may donate susceptibility to brucellosis.
Moreover these findings need confirmation in other
population groups.
doi:10.1016/j.clim.2007.03.259
F.46 Interleukin-1β(+3953) Gene Polymorphism and
Susceptibility to Helicobacter Pylori Associated
Gastritis
Ayda Hosseinkhani, Pharmacist, Clinical Microbiology
Research Center, Shiraz University of Medical Sciences,
Shiraz, Simin Kiany, MSc, Clinical Microbiology Research
Center, Shiraz University of Medical Sciences, Shiraz,
Manoochehr Rasouli, PhD, Clinical Microbiology Research
Center, Shiraz University of Medical Sciences, Shiraz, Akram
Jamshidzadeh, PhD, Faculty of Pharmacy-Shiraz University
of Medical Sciences, Shiraz, Shohreh Farshad, PhD, Clinical
Microbiology Research Center, Shiraz University of Medical
Sciences, Shiraz
Introduction: It has been speculated that IL-1 genes
play a crucial role in the genetic predisposition to gastric
problems upon H. pylori infection by modulating the host
immune response. Three biallelic polymorphisms have
been reported in IL-1 β all representing CNT base
transition at position −511, −31 and +3953 from the
transcriptional site. In the present study we attempted to
determine the IL-1 β (+3953) risk genotypes to H. pylori
mediated gastritis. Methods: The subjects were 373
individuals with no gastrointestinal problems and 49 H.
pylori positive patients suffering from gastritis which was
confirmed on the basis of endoscopic findings. All subjects
were genotyped for IL-1 β+3953 gene polymorphism using
polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP). Results: There was no significant
difference in the frequency of IL-1 β +3953 C/T
genotypes between two groups.
Discussion: The lack of significant difference in the
frequency of IL-1 β+3953 C/T between the studied groups
might be the result of our small patient population. Thus we
suggest that this study be performed on larger group of
patients; also investigation of other IL-1 functional gene
polymorphism on Iranian population is recommended.
doi:10.1016/j.clim.2007.03.260
F.48 The −251 AA Genotype of the Interleukin-8
Promoter is Associated with Susceptibility to
Brucellosis
Manoochehr Rasouli, PhD, Immunology Department, Clinical
Microbiology Research Center, Shiraz University of Medical
Sciences, Shiraz, Simin Kiany, MSc, Immunology Department,
Clinical Microbiology Research Center, Shiraz University of
Medical Sciences, Shiraz
Background: Interleukin-8 (IL-8), a member of CXC chemokine
family, is a chemoattractant of neutrophils and lymphocytes.
IL-8 promoter is estimated to be 1500 bp. Several reports
have shown relationship between IL-8 gene polymorphism and
several human diseases such as bronchial asthma, Parkinson’s
disease and Helicobacter pylori infection. The aim of this study
was to evaluate the relationship between IL-8 (−251 A/T)
polymorphism and brucellosis. Methods: One hundred and
eighty eight patients with brucellosis and 77 healthy farmers
who consumed contaminated row milk and dairy products of
animals with brucellosis were included in this study. All
individuals were genotyped for IL-8 (−251 A/T) promoter
polymorphism using polymerase chain reaction-restriction fragment
length polymorphism (PCR-RFLP). Results: The frequency
of AA genotype was significantly higher in patient group than
healthy individuals (14.3% vs. 2.5%, P=0.005). Discussion: As
data revealed that the frequency of AA genotype was lower in
healthy controls than patients. Hence, our study provides
evidence that the presence of AA genotype is significantly
associated with susceptibility to brucellosis.
doi:10.1016/j.clim.2007.03.261
F.49 Interferon Gamma Producing T Lymphocytes in
Bacterial Biofilm Infection: Response Modifiers for
Polymorphonuclear Neutrophils (PMN)
Christof Wagner, Associate Professor, University of
Heidelberg, Heidelberg, Germany, Christof Iking-Konert,
Assisant Professor, University of Duesseldorf, Duesseldorf,
Germany, Andreas Wentzensen, Head of Clinic, Klinik fur
Unfall-und Weiderherstellungschirurgie, Ludwigshafen,
Germany, Gertrud Maria Haensch, Full Professor, University
of Heidelberg Department of Immunology, Heidelberg,
Germany, Volkmar Heppert, Head of Department, Septic
Traumatology, Ludwigshafen, Germany
Bacterial infection with biofilm formation on orthopaedic
implants causes a progressive, destructive inflammatory
process, causing massive tissue destruction, bone resorption,
and loosening of the implant. In most cases, this so-called
implant-associated posttraumatic osteomyelitis requires the
removal of the implant, allowing recovery and analysis of the
local cellular infiltrate. By cytofluorometry, the majority (60–
85%) of the infiltrated cells were identified as highly activated
polymorphonuclear neutrophils (PMN); the next largest cell
population (5–35%) were T-lymphocytes. CD4 and CD8 positive T
cells were found, the latter prevailing compared to the
peripheral blood. The majority of the CD8+ cells were CD28
negative; about 26% expressed CD11b, 76% CD57. The latter two
receptors were co-expressed on less than 5% of the cells. The