Botrytis - Futureco Bioscience

YEARBOOK 2009

F U T U R E C O B I O S C I E N C E
I N D E X
Greetings from the General Manager. .................................................................... 3
Launching of the new catalogue. .......................................................................... 4
Renewal of certification for fertilizers manufacturating. .......................................... 4
First national authorization of NOFLY in Belgium .................................................... 5
New website of FUTURECO BIOSCIENCE ................................................................ 6
Begins registration process of a new biological fungicide: ACTINOVATE SP ................ 7
FUTURECO BIOSCIENCE co-sponsors publication of “Biological Control
Agricultural Pests” issued by PHYTOMA Magazine .................................................. 8
ECOLETTER: a new technical publication. .............................................................. 8
BIOGROW CORPORATION: the global distribution
network of FUTURECO BIOSCIENCE. ................................................................... 11
BIOGROW MAROC: a key strategy for BIOGROW EUROPE ...................................... 12
BIOGROW AUSTRALIA joint venture between
BIOGROW CORPORATION and INDUSTRIAL PRODUCTS MARKETING Pty Ltd. ............ 14
BIOGROW USA: a new subsidiary located in Florida .............................................. 16
BIOGROW BRASIL: leading growth of BIOGROW CORPORATION ............................. 18
FUTURECO BIOSCIENCE have successfully completed a project
for developing a new bionematicide ................................................................... 21
NOFLY: efficacy field trials, compatibility with synthetic
PPPs and side effects on natural enemies ............................................................ 24
BOTANIGARD: Efficacy field trials on Aphids, Banana black weevil and Tomato leaf
miner (Tuta absoluta). Compatibility tests with fungicides ....................................... 28
BESTCURE: Laboratory efficacy assays against fungi and bacteria,
Compatibility with biopesticides and control of Botrytis in grapes in South Africa ..... 32
7th New AG International Conference in Barcelona ............................................... 36
4th Annual General Meeting of IBMA in Switzerland ............................................. 37
The second ONTA congress in Brazil .................................................................... 38
Second symposium on formulation of microorganisms ........................................... 39

I am pleased to welcome our collaborators to the second edition of FUTURECO
BIOSCIENCE’s YEARBOOK. In line with the first edition, this Yearbook 2009
summarizes our commercial work and R&D advances of the last year.
Despite the difficult economic environment, it has been a year of sales
achievements and technical advances for FUTURECO BIOSCIENCE. This has
enforced our leading position in research, development and commercialization
of biopesticides in Europe. We received the permits from Belgian authorities
to sell NOFLY in Belgium and a dossier was submitted to Dutch Authorities for
a new bio-fungicide active substance (ACTINOVATE). Also, BOTANIGARD SC
was consolidated as a valuable tool for MIP programs in Spain, Greece and
Italy. The registration dossier has been initiated for a new bio-nematicide to
be submitted in 2010. This activity leads FUTURECO BIOSCIENCE to the top of
European companies offering registered biopesticides or in the process of Annex
1 inclusion.
At a commercial level in 2009, we continued with the expansion process of
BIOGROW creating and consolidating new subsidiaries. Our key milestone
without any doubt has been BIOGROW USA located in Florida, where
FUTURECO BIOSCIENCE’s nutritional line has been developed, registered
and commercialized. Other subsidiaries such as BIOGROW Peru, has been
constituted and BIOGROW Australia has begun its commercial activity pushed
by the technical training of its staff at central headquarters located in Chile.
BIOGROW Brazil has introduced some administrative changes to drive into a
more professional management focused on excellence and customer support.
BIOGROW Morocco has consolidated its presence in the whole Moroccan
territory by hiring four new technicians to cover the main agricultural areas of
the country. BIOGROW Morocco has also started trials with tomato hybrid seeds
and the registration of three biological plant protection products.
The global financial crisis made 2009 a difficult year for everybody. However,
FUTURECO BIOSCIENCE has been able to adapt itself to the new scenario and
keep growing. This has been possible thanks to the policy of prioritizing research
and development of “clean products” for both plant protection (biopesticides)
and plant nutrition. In 2009 new work lines have been established and a novel
microorganism with a huge antagonistic activity against nematodes has been
identified.
The success of FUTURECO BIOSCIENCE is co-responsibility of our collaborators
and customers worldwide. With your support we are aimed to keep building a
global company with a growth based on environment care and product quality.
F U T U R E C O B I O S C I E N C E
3
Mr Rafael Juncosa,
CEO FUTURECO Group
Greetings from
the General
Manager

The new catalogue of products and services of FUTURECO BIOSCIENCE
SL. was presented to our collaborators in March. The innovative design
ensures the continuous update of technical information of existing and new
products. Its flexible format will allow an easy update with the incorporation
of news, articles and most relevant results from the R&D department.
Distributors of FUTURECO BIOSCIENCE’s products have now the guarantee
of receiving our latest developments in a friendly-commercial way.
Renewal of certification
for fertilizers manufacturing
The Spanish National Regulation 824/2005 of 8 July 2005 binds those
companies dealing with manufacturing of fertilizers to have a valid certificate
issued by local authorities, or authorized third party accreditation bodies,
stating that facilities for manufacturing and storage fertilizers products are
in compliance with the obligations established in the regulation.
FUTURECO BIOSCIENCE, SL was one of the first Spanish companies audited
by an accredited third party to verify the fulfillment of the regulation. In
2009 this certification has been renewed with Bureau Veritas, currently the
only organization accredited by the public national accreditation institute
(ENAC) to carry out this type of certification.
The certification as fertilizer manufacturer guarantees the quality of the
products and services offered by FUTURECO BIOSCIENCE, SL. In cooperation
with our customers and partners, the policy of the company will remain
in the strict compliance of regulations for development of a sustainable
agriculture and safe for both environment and health of the consumers.
F U T U R E C O B I O S C I E N C E
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YEARBOOK 2009 · NEWS
MARCH
Launching of
new catalogue
APRIL

YEARBOOK 2009 · NEWS
First national
authorization of NOFLY ®
APRIL
in Belgium
The Belgian Federal Public Service of Health, Food Chain Safety and
Environmen authorized FUTURECO BIOSCIENCE to commercialize
NOFLY in Belgium since last April 16th.
NOFLY has been authorized to control two whitefly species: Trialeurodes
vaporariorum and Bemisia tabaci. The scope of registration includes
the next crops: melon, cucumber, tomato, pepper, eggplant, zucchini
and squash. These uses are supported by greenhouse trials in which
NOFLY demonstrated at least similar or greater control than the
synthetic insecticides used as a reference.
This is the first registration granted for NOFLY after more than eleven
years and around 1 million euros invested. This registration is a great
achievement for the R&D department of FUTURECO BIOSCIENCE,
S.L. since the whole process and its submission to the authorities
was carried out by the company´s R&D department without turning
to external consultancy (which is the common practice by other
companies). Likewise, FUTURECO BIOSCIENCE´s stake in research
and development with the goal to create new exclusively biological
products with minimal environmental impact has been rewarded.
This first registration is expected to be the starting point for new
authorizations currently ongoing in Morocco, Chile, USA and Spain.
F U T U R E C O B I O S C I E N C E
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New website of
FUTURECO BIOSCIENCE
FUTURECO BIOSCIENCE presents its new corporate website
www.futurecobioscience.com with a slight design change and
several improvements from the former web:
• Constant updating of contents in each section. Introduction of a
NEWS section through which customers will be informed about
the company’s activities and product developments
• New technical specifications for each product
• Activation of download tool for yearbook (news, technical
information and R&D advances).
• Launching of “JOINT US”, a project to call for researchers
interested in taking part of the R&D team and partners interested
in adhering to the BIOGROW Corporation global distribution
network.
F U T U R E C O B I O S C I E N C E
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YEARBOOK 2009 · NEWS
JUNE

YEARBOOK 2009 · NEWS
SEPTEMBER
Begins registration process
of a new biological fungicide:
ACTINOVATE SP
Last September, the Research & Development department of FUTURECO
BIOSCIENCE, S.L. completed the dossier for supporting inclusion in
Annex I to Directive 91/414/EC of the new biological control agent
Streptomyces lydicus WYEC 108. This BCA is a beneficial bacterium
belonging to Actinomycetales with recognized antimicrobial activity
on a wide range of soil decay fungi and aerial pathogens.
ACTINOVATE SP is a commercial product based on WYEC 108 strain
of S. lydicus. It is manufactured in the USA by NATURAL INDUSTRIES
Inc, and since 2007 FUTURECO BIOSCIENCE has exclusive rights for its
distribution in Europe, Chile and Morocco. Currently ACTINOVATE SP
is being applied successfully in several countries to effectively control
Pythium, Rhizoctonia, Fusarium, Phytophthora, Verticillium, mildew,
Botrytis, Alternaria and others on different crops such as vegetables,
legumes, grapes, stone and pome fruit, turf and ornamentals.
When introduced into the soil, S. lydicus WYEC 108 colonizes,
grows and proliferates around the roots by establishing a synergistic
relationship by feeding off of the plant’s waste materials while
secreting beneficial and protective by-products, forming a defensive
barrier around the root system. Furthermore, S. lydicus produces
siderophores, molecules able to complex minerals and micronutrients
found in the soil, allowing an easier uptake which provides a direct
plant growth promotion effect.
Holland has been selected as RMS for this dossier on the basis of
its experience and effectiveness as this country leaded the evaluation
of microorganisms included in fourth stage of reviewing program of
Directive 91/414/EC.
Currently, ACTINOVATE SP is registered in the USA, Canada, Turkey,
India, and New Zealand. The imminent amendments to Directive
91/414 are aimed to reduce and rationalize the use of synthetic plant
protection products and thus need for ecological alternatives for plant
protection is becoming bigger. FUTURECO BIOSCIENCE consolidates
its commitment to the development of biopesticides in the midst of
the crisis caused by the withdrawal from the market of many synthetic
active substances.
F U T U R E C O B I O S C I E N C E
7

FUTURECO BIOSCIENCE co-sponsors
publication of “Biological Control of
Agricultural Pests” issued by
PHYTOMA Magazine
The delivery of fascicles from the book “Biological Control of
Agricultural Pests” by J.A. Jacas and A. Urbaneja (Associated Unit of
Entomology UJI-IVIA-CIB) issued by Phytoma magazine with special
collaboration of FUTURECO BIOSCIENCE SL, was completed last
September.
The fascicles were were delivered monthly monthly since June 2008 along with with the
Phytoma Phytoma Magazine. The complete complete book contains contains 496 496 pages pages that
focus focus on general biocontrol biocontrol matters and the complete description of
the biological control agents of the main agricultural pests in Spain,
with with contributions from from a large number of specialists. The book is
illustrated illustrated with high quality images included in the individual sections.
This publication will be a powerful powerful and useful tool for technicians and
farmers.
ECOLETTER: a new
technical publication
FUTURECO BIOSCIENCE started publishing a new technical brochure
called ECOLETTERs since May 2009. The aim of this publication is to
bring to distributors and customers the latest information on scientific
results of trials using our products.
The technical data used for ECOLETTERs is derived from internal
assays conducted at our laboratory and field trials carried out by
our collaborators or official research institutions for registration
purposes.
The ECOLETTERs will be published with undefined periodicity, issued
as soon as a new study with remarkable results has been completed.
The ECOLETTERS can be downnloaded from the PUBLICATIONS
section of our website. The printable format is designed for being
included within the binder of our catalogue as supporting material of
the tested products.
F U T U R E C O B I O S C I E N C E
8
YEARBOOK 2009 · NEWS
SEPTEMBER
DECEMBER

F U T U R E C O B I O S C I E N C E
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F U T U R E C O B I O S C I E N C E
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YEARBOOK 2009 · SUBSIDIARIES
BIOGROW CORPORATION:
the global distribution network of
FUTURECO BIOSCIENCE
www.biogrow.cl
BIOGROW COPORATION was created for the distribution and
commercialization of products for Plant Protection and Plant Nutrition
with the guarantee of FUTURECO BIOSCIENCE. The catalogue of
BIOGROW includes Bio-stimulants, Foliar and Soil Mineral Deficiency
Correctors, Plant Defenses Activators, Phosphites, Coadjuvants,
Bio-fungicides and Bio-insecticides.
BIOGROW CORPORATION is the result inspired by the acquired
experience in the manufacturing, development and commercialization
of products for plant protection and plant nutrition of FUTURECO
BIOSCIENCE in Europe, North Africa and Middle East and from
experience of BIOAMERICA in Chile. BIOGROW Corporation is
nowadays present through subsidiary companies and local network
of distributors in North America, South America, Europe, Oceania and
Africa.
BIOGROW COPORATION is committed to bring added value to crop
production by offering products and programs designed to improve
yields and overcome adverse agronomical situations. BIOGROW
CORPORATION works in close cooperation with local Research
Centers and Universities to develop new applications of its products
in each country. The staff of BIOGROW CORPORATION is composed
of highly trained professionals in the application of products over the
most representative crops.
The objective of BIOGROW CORPORATION is to improve nutritional and
phytosanitary condition of crops by developing and commercializing
innovative and environmental friendly products within a policy of
close cooperation with farmers.
F U T U R E C O B I O S C I E N C E
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Mr. Paolo Solari
General Manager BIOGROW CORPORATION

BIOGROW MOROCCO: a key strategy
for BIOGROW EUROPE
Mr. Jamal Bourakhouadar
General Manager Biogrow Maroc
YEARBOOK 2009 · SUBSIDIARIES
BIOGROW MAROC Sarl started operating in mid-2007 with some
basic objectives including:
• Extend and strengthen the commercial network of BIOGROW
EUROPE by establishing a subsidiary in one of the most promising
and demanding agricultural markets: Morocco
• Active participation in the “Green Morocco Plan”, which aims to
boost horticultural and olive crops in Morocco
• Provide a wide range of environmental friendly products to meet
farmers needs
BIOGROW MAROC is a young company with a sales team of 2
agricultural technicians and 2 agronomic engineers. BIOGROW´s
activity is developed in the main Moroccan agricultural areas such
as Souss (Agadir, Taroudante and Dakhla), Central (Marrakech, Beni
Mellal, El Jadida) and North east (Berkane, Fez, Meknes and Midelt).
BIOGROW MAROC has quadrupled its turnover in two years, becoming
one of the leading Moroccan companies. Considering the importance
of the market, it has designed a strategic sales plan that will allow to
increase 50% 2009´s turnover, meaning, they will multiply by seven
the results achieved in 2008. In order to meet this plan an agronomist
engineer will be incorportated as a new staff member to cover the
North West area (Larache, Sidi Kacem, Laawamra).
BIOGROW increased its products offer in 2009
BIOGROW MAROC sells a wide range of products focused in plant
protection and plant nutrition, all of them classified as low risk
substances for the environment. Currently the catalogue of products
of BIOGROW includes:
• Biostimulants
• Plant strengtheners
• Mineral deficiency correctors
• Soil conditioners
F U T U R E C O B I O S C I E N C E
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YEARBOOK 2009 · SUBSIDIARIES
The most relevant Biostimulants are BIO ROOT POWER (rooting),
ECOLIV (for improvement of setting and fattening of fruits), KINESTIM
(to increase yields) and GREENSTIM (biostimulant formulated with
betaine to help plants to recover from stress situations). These products
have shown excellent results on several crops, especially vegetables
and fruit trees.
To extend the catalogue, BIOGROW MAROC is working on
registering biological products based on microorganisms and vegetal
extracts directed to plant protection. It is expected to complete the
homologation process for three of these products in 2010.
To support commercial activities, different efficacy trials are ongoing on
four tomato varieties in prestigious nurseries in “JANAH ESSALAM”.
Partial results on undefined tomato varieties have showed excellent
results. The trials on determined varieties will start next February
2010.
BIOGROW MAROC’s commitment with the “Green Morocco Plan”
The objective of “Green Morocco Plan” is to boost development of
Moroccan agriculture with support of foreign private capital. The
main actions proposed by this plan are focused on the progressive
substitution of current cereal and forage by alternative crops with
higher added value such as olive trees and vegetables. The “Green
Morocco Plan” will privatize 700.000 Ha that will be transformed into
more profitable and intensive crop lands, incorporating innovative
technologies for irrigation systems.
The “Green Morocco Plan” entails implementation of around 700 to
900 projects with budgets ranging from 10 to 15 thousand millions of
dirhams (about 1300 million euro), making necessary private, national
and foreign investments. The “Green Morocco Plan” expects to turn
agriculture into the driving force of social and economic development
of the country. In parallel, the government has signed a contract –
program with professionals of the olive oil sector destined to the
plantation of 540.000 ha of olive trees aimed to reach 1.220.000 ha
by 2020 to produce over 340.00 tons of oil. The foreseen investment
is 6 thousand millions of dirhams for the olive oil sector while for
vegetable crops it will be around 12 thousand millions.
BIOGROW MAROC aims to be part of the “Green Morocco Plan”
by offering low risk products for plant protection and plant nutrition
without having an adverse impact to the environment as it has
occurred in other countries.
F U T U R E C O B I O S C I E N C E
13

BIOGROW AUSTRALIA joint venture
between BIOGROW CORPORATION
and INDUSTRIAL PRODUCTS
MARKETING Pty Ltd.
www.biogrow.com.au
Mr Geoff Jones, General Manager of
BIOGROW AUSTRALIA Pty Ltd
YEARBOOK 2009 · PARTNERS AND DISTRIBUTORS
Australia is a vast desert continent, with an agriculture industry biased
towards livestock and broad-acre (cereals) farming. On the other
hand, horticulture is considered to be the fastest growing sector
within agriculture in Australia. Australian horticulture annually exports
approximately A$750million worth of food and employs more than
100,000 people. Horticultural activity is mostly located in the States
of Victoria, Southern NSW and selected regions along the east Coast
of Queensland.
Activities of Biogrow Australia in 2009
February 2009 saw the birth of BIOGROW AUSTRALIA Pty Ltd – a
joint venture company between BIOGROW Corporation and Industrial
Products Marketing Pty Ltd. Its first field office was established
in Mildura, Victoria, in the centre of one of the most significant
horticulture regions for Australia, known locally as Sunraysia.
Sunraysia has a focus on citrus, tablegrapes, winegrapes, almonds
and vegetables.
Biogrow Australia Pty Ltd has hired Shane Singh to begin the first sales
for season 2009-10 and build on the brand of BIOGROW AUSTRALIA
as a new an innovative supplier of nutrition and bio control products.
Shane has worked on both sides of the fence with respect to farm
nutrition management and then moving into agronomy product sales
with distributors such as Elders; Australia’s oldest and largest distributor
of products and services into agriculture. Shane is also educated with
a degree in rural science, holding him in good stead with respect to
the technical aspects of BIOGROW AUSTRALIA’s products.
Sales are currently focused upon the nutritional range of foliar
fertilisers, with biostimulants already introduced into trials for grower
approval. CITOGROWER has shown early promise in Citrus varieties
with respect to flower retention and quality. There is a lot more work
to be done, but early indications are promising.
F U T U R E C O B I O S C I E N C E
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YEARBOOK 2009 · PARTNERS AND DISTRIBUTORS
Participation in National Fairs
The Victorian strawberry, blueberry and raspberry association held
a trade show earlier in the season in which BIOGROW AUSTRALIA
participated as a bronze sponsor to launch the profile of BIOGROW
AUSTRALIA for that region.
Using customer knowledge of AMINOSTIM is leverage, BIOGROW
AUSTRALIA was able to make early penetration into this market
segment as well as pome and stonefruit varieties.
F U T U R E C O B I O S C I E N C E
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Stand of BIOGROW AUSTRALIA in the trade
show Victorian strawberry, blueberry and raspberry
association.

Mr Xavier Vivanco, General
Manager of BIOGROW USA
YEARBOOK 2009 · SUBSIDIARIES
BIOGROW USA: a new
subsidiary located in Florida
www.biogrow-usa.net
BIOGROW USA was created in October 2008. The central office is
located in Central Florida, the area that concentrates the highest
production of vegetables and fruits in the state.
In March 2009, the Florida Department of Agriculture authorized the
commercialization of the first products: BIORADICANTE, FRUTALIV,
VITALEM FORTE, ALGALIV, FOSFIMAX 30-20 and ECOSALT. In order
to promote sales, during 2009, technical visits to local distributors and
principal farmers of tomato, citrus and strawberry were scheduled. At
the same time, several efficacy demonstration trials were contracted
with the Agronomic Research Institute of the University of Florida in
Baum.
Technical Seminar planned by BIOGROW USA
A technical seminar was held at Cleveland Heights Golf Club (Lakeland,
Florida) organized to launch BIOGROW USA in EEUU. The event was
attended by local farmers and potential distributors. The speakers
were Mr Paolo Solar, General Manager of BIOGROW CORPORATION,
Mr José Manuel Lara, Technical Manager of FUTURECO BIOSCIENCE
and Mr Xavier Vivanco, General Manager of BIOGROW USA. The
seminar covered the following topics:
• Introduction of BIOGROW USA
• Presentation of FUTURECO BIOSCIENCE, the manufacturer and
quality supporter of BIOGROW’s products.
• Characteristics of BIOGROW’s products: mode of action and
efficacy examples.
Efficacy field trials in cooperation with University of Florida
A trial on tomato plants supervised by Dr Bielinski Santos was run
from August 2009 in the Agricultural Research Institute of University
of Florida in Baun. The study included the following treatments:
Group 1 plants: Control
Group 2 plants: Vitalem Forte
Group 3 plants: Vitalem Forte and Frutaliv
Group 4 plants: Vitalem Forte, Frutaliv and Bioradicante
F U T U R E C O B I O S C I E N C E
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YEARBOOK 2009 · SUBSIDIARIES
The best results were obtained with plants treated with Vitalem Forte,
Frutaliv and Bioradicante (group 3). The plants showed increased
nitrogen retention capacity in leaf, 8.9% increase fruits with extralarge
diameter and 21% increase in fruits with large diameter.
Besides, this group yielded 11% more marketable fruits than control
treatment (in tons / acre).
A new trial is planned to start in February 2010 and will target the
efficacy of products to help plants in overcoming effects of an artificially
induced stress (reduction of Nitrogen fertilization or irrigation water).
Registration of products in California
California is the major USA agricultural market (approximately
50% higher than Florida), and consequently there is a big interest
of introducing BIOGROW’s products in this state. The homologation
process for label authorization started in October 2009, although
the strict legislation for commercialization of fertilizers will make
it long and complex. However, Californian farmers are, in general,
open minded and committed with organic agriculture which may
help to welcome BIOGROW’S products on basis of their efficacy and
environmental friendly profile.
Participation of BIOGROW USA in Expo 2009
BIOGROW USA had a commercial stand during the agricultural AG
Expo held in October 2009 in Baum, Florida. Over 1200 people
attended the fair, included the main distributors and manufacturers of
agricultural inputs. Different seminars on novel agricultural techniques,
irrigation systems, and other relevant technologies were presented.
Walt Disney Corporation visited the stand of BIOGROW USA. This
company, through Epcot Science, is currently evaluating the efficacy
of BIOGROW products for application in hydroponic systems.
Strategies for 2010
One of the main objectives for 2010 is to extend the catalogue of
products with mineral deficiency correctors (DEFENDER brand line).
The Florida soils are characterized by high levels of potassium, iron
and phosphorous. The application of new BIOGROW’S products
targets an optimal utilization of these minerals by plants, improving
nutrition and crop yields. An other product that will be included in
BIOGROW’S catalogue is the biostimulant CITOGROWER, which
has strong potential for citrus crops fertilization programs. However,
biostimulants require federal registration (EPA) and therefore the
availability of CITOGROWER for BIOGROW’s customers may take
some time.
F U T U R E C O B I O S C I E N C E
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Figure 1:
Technical visit within AG Expo in Florida
Figure 2:
Technical seminar organized by BIOGROW USA
Figure 3:
Field efficacy trial with BIOGROW USA products
carried out by University of Florida (Baum, FL, USA)

BIOGROW BRASIL: leading growth of
BIOGROW CORPORATION
www.biogrow.com.br
Mr. Alejandro Gesswein,
General manager of BIOGROW BRASIL
YEARBOOK 2009 · SUBSIDIARIES
BIOGROW BRASIL, one of the first subsidiaries of BIOGROW
CORPORATION, was created in 2007 with the huge challenge of
becoming part of the biggest and more demanding Latin-American
market: Brazil. The office was established in Petronila, Pernambuco in
the heart of one of the regions with highest technical development
for crop management and extension.
A close cooperation between technical departments of FUTURECO
BIOSCIENCE in Spain and BIOGROW in Brazil made possible to
obtain registration for 12 products in a very short time in 2008. The
authorization for commercialization from the Ministry of Agriculture
was granted for all biostimulants of FUTURECO BIOSCIENCE’s catalogue
and some mineral deficiency correctors. In the last year almost all of
these products have been introduced in the agronomic programs for
management of fruit crops, highlighting Grape, Watermelon and
Mango. This has been possible by transferring the valuable experience
of a highly skilled team of agronomists from Bioamerica in Chile.
BIOGROW BRASIL activities in 2009
The activity of BIOGROW BRASIL during 2009 was mainly focused in
the consolidation of a niche market created the previous year. One of
the most remarkable successes was the introduction of a complete
nutritional program for Grape and Mango crops with outstanding
products as BIORADICANTE, VIGORAL MOL and CITOGROWER which
increased their sales over the previous year.
In 2009, registration was achieved by a dozen other products,
including mineral deficiency correctors and new formulations such
as ALGALITHO, a liquid concentrate of seaweed and microelements
with a large market potential. This registration allows starting
commercialization of products with tremendous potential for Brazilian
market such as BESTCURE and ALFOSITOL.
The distribution network was substantially increased to cover a more
extensive agricultural area, allowing an increase of the company’s
total sales by 33.5% over the year 2008.
Forecasting of BIOGROW BRASIL for 2010
The company has set a number of goals for 2010 to take advantage
of the big potential of the Brazilian agricultural market. One of the
key actions for being successful in these challenges is having a
good management, and BIOGROW BRASIL has decided to appoint
a new general manager. The person in charge of BIOGROW BRASIL
F U T U R E C O B I O S C I E N C E
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YEARBOOK 2009 · SUBSIDIARIES
since November 2009 is Mr Alejandro Gesswein, who possess a
strong knowledge of the Brazilian market. His career started in
1988 in Bayer Corporation, Chile. After 20 years as business
manager of multinational companies and having lived in a number
of countries, he is now integrated within BIOGROW’s family with
clear objectives.
The year 2009 was full of uncertainties derived from the global
economic crisis. For 2010 and after our very successful first national
sales meeting, the objective is to increase sales 100%.
The achievement of our sales goal requires a demand be created in
new sectors. For this target, technical projects have been defined
in both universities and commercial fields. The studies are aimed
to validate, endorse, disseminate and promote plant nutrition and
plant protection programs based on BIOGROW’s products. The
projects are specifically drawn to boost the more representative
products on relevant crops. Some of the trials scheduled for early
2010 are:
• Control of bacterial diseases with BESTCURE and ALFOSITOL
in tomato and Melon
• Nutritional and Protection assessment in plant protection
programs including in Melon, Watermelon and Potato
• Evaluation of DEFENDER line (mineral deficiency correctors) in
Grape and Mango
• Dose-range study of CITOGROWER in table grapes
• Control of Antracnose in Mango
In addition to commercial trials, BIOGROW’s strategy for growth is
to consolidate a powerful distribution channel, with the creation
of strategic alliances for new markets and extending the portfolio
of BIOGROW’s products.
These activities are expected to push sales, which in combination
with a controlled expenditure budget, will contribute to obtain
profits needed by BIOGROW CORPORATION to continue its
expansion.
F U T U R E C O B I O S C I E N C E
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Figure 1:
Standard management (left) and BIOGROW
plant protection program (right)
BIOGROW Brasil collaborates with ABRINQ
Foundation (www.fundabrinq.org.br)

F U T U R E C O B I O S C I E N C E
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YEARBOOK 2009 · ADVANCES IN R&D
FUTURECO BIOSCIENCE have successfully
completed a project for developing a new
bionematicide
1 2
3 4
A three-year industrial research project (CIDEM) entitled “Preliminary
development of a microbial pest control product for the biological
control of plant parasitic nematodes” led by FUTURECO BIOSCIENCE,
was successfully completed last December. The project was
developed in cooperation with the Department Agricultural and Food
Engineering and Biotechnology of Polytechnic University of Catalonia
and the Fermentation Pilot Plant of UAB University. The aim of the
study was the preliminary development of a biological tool based on
Metharhizium anisopliae entomopathogenic fungus for control of
banana black weevil.
The fist stage of the project consisted of soil sampling in some Spanish
regions (Murcia, Barcelona, Tarragona and Eivissa). The samples of
soils and roots infected with plant parasitic nematodes were subject
to extractive procedures to obtain microbial flora. A first screen yielded
246 fungi and 82 bacteria that were further growth on selective
F U T U R E C O B I O S C I E N C E
21
Figure 1:
Bacterial strain Q29A with bionematicide potential.
Figure 2:
Female of the cyst nematode Globodera sp
Figure 3:
Juvenile of the root knot parasitic nematode
Meloidogyne sp
Figure 4:
Group of eggs of Meloidogyne sp. In different
development stages

5
Figure 5:
Root system of tomato plant heavily infested with galls
from Meloidogyne sp.
Figure 6:
Tomato root with 2 galls, one of them parasited by a
possible antagonistic fungus
YEARBOOK 2009 · ADVANCES IN R&D
media to isolate 109 potential microbial Biological Control Agents
(BCAs). The strains were purified, morphologically characterized and
ultra-frozen (- 86 ºC).
In the next stage, the isolated BCAs were evaluated on Meloidogyne
sp eggs using a novel in vitro technique. It consisted on 1 mL wells with
egg masses of Meloidogyne inoculated with aqueous suspensions of
microorganisms. From the potential BCAs evaluated, a total of 10
microorganisms showed egg mortality above 75%, and the 3 out
them showing the higher activity were selected for the next stage of
the project.
The third part started with preparation of 45 crude prototypes of
formulations (CPF) using 3 fermentation systems and 5 different
fractions (including solvent extraction of biomass and broth) from the
3 microorganisms selected. The 45 CPFs were tested again in vitro
on egg masses. The analysis of experimental results (statistical) in
conjunction with yield in fermentation systems of each CPF, ended
with selection of the CFP with best global performance to develop
a further technical prototype. It was the one based in a bacterium
coded as strain FEBN 0308 Q29A.
This selected strain was used as active ingredient for preparation of
eight formulations, that were physical and chemically characterized
and their stability along time evaluated at different temperatures (4,
35 and 45ºC).
The efficacy of 2 formulations was evaluated in tomato plants variety
Durinta, infected with 100 juveniles of the root knot nematode
Meloidogyne javanica. The trial was carried out in a climatic chamber
(25±2ºC, dark:light period 18h:6h). Each formulation was applied
twice: 2 days after nematode inoculation and at transplantation (7
days after inoculation).
F U T U R E C O B I O S C I E N C E
22
6

YEARBOOK 2009 · ADVANCES IN R&D
Both formulations effectively reduced infection, reproduction and
fertility of nematode respect to control (Table 1). The strain used in the
formulation showed hatching inhibition capacity (90%). No adverse
effects were seen in the treated plants derived from applications of
the formulation or aqueous suspension of the microorganism.
The results of the project have a sound foundation for further
development of a commercial biopesticide. Although more studies are
still needed to meet the legal requirements by the authorities in this
type of product (basic testing toxicology, ecotoxicology, field efficacy
trials, etc.). In the future banana producers in the Canary Islands may
have an ecological solution to the problem of the borer, based on
their own biodiversity
Additional studies are necessary to complete the development of a
commercial bionematicide. However, the results of this project are
promissory and represent a new tool for ecological management of
plant parasitic nematodes based on national biodiversity. Therefore
during 2010 the Research and Development department of FUTURECO
BIOSCIENCE will continue working on optimizing the formulation
and evaluation on other species of plant parasitic nematodes under
controlled conditions (climatic chamber).
INFECTIVITY
Nº egg masses/plant
F U T U R E C O B I O S C I E N C E
23
REPRODUCTION
Nº egg masses/plant
Figure 7:
Egg of a nematode parasited by a fungal BCA
Table 1. Percentage of infectivity, reproduction and fertility at the end of the trial (2 months)
FERTILITY
Nº egg/egg mass
CONTROL 100% 100% 100%
Strain Q29A 19.28% 8.3% 14.3%
Formulation Nº 7 24.25% 6.47% 23.18%
Formulation Nº 8 8.96% 1.43% 1.61%

YEARBOOK 2009 · ADVANCES IN R&D
NOFLY: efficacy field trials, compatibility
with synthetic PPPs and side effects on
natural enemies
Table 1. Efficacy trials of NOFLY in 2009
During 2009, the R&D Department of FUTURECO BIOSCIENCE has
worked extensively in the commercial development of the new
biopesticide FUTURECO NOFLY (Paecilomyces fumosoroseus strain
FE9901), to support its utilization as an effective tool within Integrated
Pest Management programs. To this effect, a number of field efficacy
trials have carried out, and its compatibility with other plant protection
products and beneficial organisms was tested.
Efficacy field trials
A total of 3 efficacy field trials were carried out with NOFLY under
different climatic conditions:
CROP TARGET PEST MAIN RESULTS
Georgia, EEUU Tomato Frankliniella occidentalis
Murcia, SPAIN Tomato var.“Óptima”
Murcia, SPAIN Pepper var. “Urano”
Whitefly
Eggs (E),
Larvae (L),
Whitefly
Eggs (E),
Larvae (L),
F U T U R E C O B I O S C I E N C E
24
Efficacy after three applications:
46% damage reduction (scars)
57% less adults an immature stages per
leaf than control
Efficacy after three applications:
NOFLY1(300 g/HL): 41% H; 65% L
NOFLY2(500 g/HL): 46% H; 79% L
STANDARD: 67% H; 91% L
Efficacy after three aplications:
NOFLY1(300 g/HL): 6% H; 61% L
NOFLY2(500 g/HL): 49% H; 76% L
STANDARD: 57% H; 85% L

YEARBOOK 2009 · ADVANCES IN R&D
Compatibility tests with synthetic plant protection products
The compatibility of NOFLY with 14 fungicides and 7 insecticides was
evaluated in laboratory tank mixtures. The test consisted of mixing a
suspension of spores of Paecilomyces fumosoroseus strain FE 9901
with the products at three rates (minimum dose, maximum dose and
double maximum dose) and then evaluating the germination ability of
spores (viability) after 0, 4 and 24 hours.
NOFLY showed to be 100% compatible with the tested insecticides
(Beauveriua bassiana, Natural Pyrethrins, Pirimicarb, Imidacloprid
and Acrinatrin) and with 5 of the fungicides (Copper oxychloride
38%, Propamocarb, Kasugamicin, Procymidone and potassium
phosphate).
NOFLY was not compatible with copper oxychloride 50%, Fenarimol,
Iprodione, Triadimenol, Myclobutanil, Triflumizol, Chlortalonil,
Thiophanate Methyl, Mancozeb, Bupimirate and Sulfur.
Side effects of formulation on beneficial insects
The effects on non-target and beneficial insects of the bioinsecticide
NOFLY have been evaluated on some of the most representative
species: Encarsia formosa, Orius laevigatus, Macrolophus caleginosus
and Eretmocerus mundus under laboratory conditions. The product
has showed to be harmless in most of the cases or slightly toxic on
immature stages of some these insects. The product has also showed
to be harmless on honeybee adults by oral and contact exposition.
During December 2009, additional studies on the predatory mite
Amblyseius swirskii were carried out. This beneficial organism is one
of the most extensively used for whitefly control. Extended laboratory
tests were also run on the beneficial insects Macrolophus caliginousus
and Orius laevigatus following internationally approved guidelines in
a GLP laboratory.
In the study on Amblyseius swirskii, exposure to Dimethoate resulted
in no eggs hatching throughout the 7 day experimental period.
In comparison, exposure to NOFLY, heat treated NOFLY and the
F U T U R E C O B I O S C I E N C E
25
Figure 1 and 2:
Germinated and ungeminated blastospores of P.
fumosoroseus under scanning electron microscope
(SEM)
Figure 3:
Blastospores of the fungus P. fumosoroseus at different
germination stages dyed with lactophenol blue
Figure 4:
Eretmocerus mundus male (right)
and female (left)

Figure 5:
Paecilomyces fumosoroseus blastopores in different
stages of germination, sataines with lactophenol.
YEARBOOK 2009 · ADVANCES IN R&D
untreated control resulted in all eggs hatching after 3 days (Graph 1).
Further evaluations showed that exposure to Dimethoate resulted in
significantly higher numbers of affected (knocked down and dead)
nymph (Graph 2) and adult (Graph 3) mites (averages of 96% and
100%, respectively) compared with NOFLY, heat treated NOFLY and
the water control (averages of 2%, 1% and 2%, and averages of
1%, 1% and 1%, respectively). It can be concluded that exposure to
NOFLY and heat treated NOFLY had no effect on the numbers of adult
and nymph mites, in terms of knockdown and mortality.
In the study on M. caliginousus, exposure to Dimethoate resulted in
significantly higher numbers of affected (knocked down and dead)
bugs (average of 65%) compared with NOFLY and water control
(averages of 10% and 15%, respectively), over a 7 day experimental
period (Graph 4). In the study on O. laevigatus exposure to standard
resulted in significantly higher numbers of affected (knocked down
and dead) bugs (average of 65%) compared with NOFLY and
water control (averages of 5% and 7%, respectively), over a 7 day
experimental period. It can be concluded that exposure to NOFLY
had no effect on the knockdown or mortality of M. caliginosus and
O. laevigatus in an extended laboratory test system, as previously
showed in laboratory assays (Graph 5).
A new study aimed to evaluate the effects of NOFLY on pollinators
under real conditions (greenhouse) will be run during 2010. The
objective of this work is to extend the information to support the
fitness of NOFLY for being included in Integrated Pest Management
programs.
F U T U R E C O B I O S C I E N C E
26

YEARBOOK 2009 · ADVANCES IN R&D
Graphic 2:
Number of affected (knocked down and dead) A. swirskii nymphs (out of 20)
exposed to treatments over a 7 day experimental period (means ± standard errors,
n = 4)
Graphic 4:
Number of affected (knocked down and dead) M. caliginosus (out of 10) exposed to
treatments over a 7 day experimental period (means ± standard errors, n = 6)
F U T U R E C O B I O S C I E N C E
27
Graphic 1:
Number of hatched
A. swirskii eggs (out
of 10) exposed to
treatments over a 7 day
experimental period
(means ± standard
errors, n = 4)
Graphic 3:
Number of affected (knocked down and dead) A. swirskii adults (out of 20) exposed to
treatments over a 7 day experimental period (means ± standard errors, n = 4)
Graphic 5:
Number of affected (knocked down and dead) O. laevigatus (out of 10) exposed to
treatments over a 7 day experimental period (means ± standard errors, n = 6)

Figure 1:
Panorama aphid control trial on lettuce after third
application of BOTANIGARD in Benifaió (Valencia)
YEARBOOK 2009 · ADVANCES IN R&D
BOTANIGARD: Efficacy field
trials on Aphids, Banana black
weevil and Tomato leaf miner
(Tuta absoluta). Compatibility
tests with fungicides
BOTANIGARD is a biological insecticide registered in Spain (ROPF
22.648), for the control of whitefly in solanaceae (tomato, pepper…)
cucurbitaceae (cucumber, squash, melon….) and cotton. Applications
are recommended every 4-7 days at a rate of 1.0 – 1.5 L/Ha. To gain
control efficacy it is preferable to increase frequency rather than rate
of applications. BOTANIGARD does not leave residues on crops and is
compatible with beneficial organisms as well as most of insecticides,
coadjuvants and some fungicides.
Throughout 2009, several demonstrative field trials were carried out in
cooperation with local distributors, farmer organizations, and in some
cases, official research centers. The main objectives for BOTANIGARD
marketing during 2009 were:
1) To extend applications of the product (pests and crops other than
those currently authorized), trough verification with field trials in
Spain the results from published studies abroad and from in vitro
bioassays from FUTURECO BIOSCIENCE’s R&D laboratory.
2) To evaluate the compatibility of BOTANIGARD for tank mixture
with other synthetic plant protection products (common
insecticides and fungicides).
F U T U R E C O B I O S C I E N C E
28

YEARBOOK 2009 · ADVANCES IN R&D
Efficacy of BOTANIGRAD for controlling banana black weevil
(Tenerife, Spain):
The entomopathogenic fungus Beauveria bassiana has been widely
reported as Biological Control Agent of the banana black weevil
(Cosmopolites sordidus) in both, in vitro and field assays.
A field trial was carried out in Tenerife (Canary Islands) in cooperation
with a local distributor (Canarias Explosivos, SA) and the Agricultural
Cooperative FAST in February 2009. The objective was to assess the
effect of the product at different doses.
The synthetic plant protection products are commonly applied
through injection to the base of the plant (Figure 2). This application
route would favor key conditions for a successful fungus development
(active ingredient of BOTANIGARD) like absence of solar radiation,
high humidity and stable temperature within the stem.
BOTANIGARD was tested at rates of 0.04%, 0.075% and 0.15%
and the standard chemical product (Clorpyrifos 48%) was applied
at 0.2% (commercial dose). The products were applied on July 15
(2009) and the adult population was monitored until the end of the
study (November 2009) every 2 weeks by trapping.
The number of collected adults / trap in the treatment with
BOTANIGARD at the highest dose (0.15%; 150 cc/hL) was below 10
throughout the trial (Graphic 1). BOTANIGARD at 0.15% was the
most effective and lasting treatment. Its performance was similar and
even better in some dates, than the standard treatment.
Efficacy of BOTANIGARD® to control aphids
Control of aphids is being seriously affected by withdrawal of
authorized active substances. The expansion and virulence of this
pest make critical to find new and low risk media to control it and
avoid significant losses in the affected crops. In this context, 2 field
efficacy field trials with BOTANIGARD were carried out on two species
of aphids in Spain during March 2009.
a. Greenhouse trial on pepper (Campo de Cartagena).
This work was carried out in cooperation with the technical team of
Fitopalma, S.L. The target crop was ecologic pepper type “California”
in greenhouse. The aphid species present were Myzus persicae and
Macrosiphum euphorbiae.
BOTANIGARD was applied at a commercial rate of 0.15%. Three
applications were done in 2 weeks (10, 13 and 20 March). Adult
counts were done on 7 randomly selected plants from each treatment,
and evolution of eggs, nymphs and adults was evaluated following
each application of BOTANIGARD.
The continuous application of sulfur overnight prevented assessing
the residual action of spores. However, the results of applications
suggested a knock-down effect on both aphid species. Hence,
F U T U R E C O B I O S C I E N C E
29
Figure 2:
Field trial on banana crop Musa x paradisiacal variety “Peuqeña nana”
(Farm in Puerto la Cruz, Tenerife). Application of Botanigard on trunk

Figure 3:
Individuals of aphid Nasonovia ribisnigri on lettuce
plants before application of BOTANIGARD
Figure 4.
Treatment of lettuce plants affected by aphids with
BOTANIGARD
Figure 5.
Evaluation of aphid level (alive / dead) after treatment
with BOTANIGARD
YEARBOOK 2009 · ADVANCES IN R&D
BOTANIGARD has demonstrated to be an effective alternative for
biological control of aphids.
b. Field trial on lettuce (Benifaió, Valencia)
In a second trial, BOTANIGARD was evaluated for control of Nasonovia
ribisnigri in an experimental open field plot cultured with lettuce
variety cv. Francesa (Fig, 1 and 3).
The experimental design consisted of random blocks (according to
level of pests) with four replicates per treatment. The elemental plot
was 9 m2. The products sprayed were:
1. CONTROL water
2. BOTANIGARD: at 3 rates: 50 mL/100 L; 100 mL/100L ; 300 mL/100L
3. STANDARD: Imidacloprid 20 g a.i./L))
The products were applied with a manual equipment “Maruyama”
(15 bar), spraying between 500 and 600 L/Ha of broth. The pest level
was determined before each application and after 7 days of the last
application. The data were analyzed through Student-Newman-Keuls
test.
The plants treated with BOTANIGARD showed 53%, 65% and 68%
less individuals (aphids) (according to each dose) than the control
treatment 7 days after last application. The standard reference showed
90% efficacy.
Efficacy of BOTANIGARD® to suppress tomato leaf miner (Nijar, Almeria):
BOTANIGARD had showed efficacy to suppress Tuta absoluta in the
last season. The treatments caused considerable reduction of larvae
activity, stopping the damage of the insect on the crop.
A new field efficacy on tomato crop in greenhouse was carried out
in October, in cooperation with technical staff of the local distributor
“Agroquímicos Céspedes”. The tests consisted of applications of
BOTANIGARD mixed with Bacillus thuringiensis in plots with different
levels of pest.
A noticeable reduction of pest level in the area treated with
BOTANIGARD respect to CONTROL was observed. The number of
adults captured in pheromone traps in the treated area was significantly
lower than that of untreated area, and the same happened with
number of galleries caused by larvae.
F U T U R E C O B I O S C I E N C E
30

YEARBOOK 2009 · ADVANCES IN R&D
Compatibility tests with other plant protection products:
The compatibility of BOTANIGARD with 26 fungicides and 7 commercial
insecticides was tested trough tank mix simulation in laboratory. A
suspension of spores of Beauveria bassiana strain GHA was mixed with
3 concentrations of each commercial product (minimum, maximum
and twice maximum doses). Then, the germination of spores was
measured after 0, 4 and 24 hours.
The results showed that BOTANIGARD is compatible with all tested
insecticides (Table 1) and 14 fungicides (Table 2) while it can not be
tank mixed with 12 out the 26 fungicides evaluated (Table 2).
Table 1. Effect of different commercial insecticides on viability of
spores of Beauveria bassiana GHA
COMPATIBLE
TRADE NAME ACTIVE SUBSTANCE
Aphox Pirimiricarb 50%
Cordalene Bacillus thuringiensis kurstaki 24%
Confidor Imidacloprid 20%
Naturalis Beauveria bassiana
Orystis Acrinatrin
Perlite-Hort Piretrinas naturales
Plenum Pimetrocina 25%
Table 2. Compatibility of commercial fungicides with BOTANIGARD
COMPATIBLE INCOMPATIBLES
TRADE NAME ACTIVE SUBSTANCE TRADE NAME ACTIVE SUBSTANCE
Bayfidan Triadimenol 25% Alcior Clortalonil
Caddy 10 Ciproconazol Antimildiu triple
Cupraflow Oxicloruro de cobre 38% Bestcure Extracto de cítricos
F U T U R E C O B I O S C I E N C E
31
Fosetil Al 50% + Folpet 25% + Cimoxamilo
4%
Driza Procimidona 50 Cupragrex Oxicloruro de Cobre 50%
Enovit-Metil Metil Tiofanato Cuprebel
Fosfimax Fosfito potásico Ditiver Tiram 80%
Oxicloruro de Cobre 22% + Mancozeb
17%
Nimrod-Quattro Bupirimato 2.5% Elosad GD Azufre mojable 80%
Previcur Propamocarb Kasumin Kasugamicina
Rovral Aquaflo Iprodiona Sofrene Azufre
Rubigan Fenarimol 12% Switch Ciprodinil 37.5% + Fludioxonil 25%
Scala Pirimetamil 40% Trifmine Triflumizol 30%
Score Difenoconazol 12.5% Mancozeb Mancozeb
Systhane forte Miclorbutanil 24%
Topas (24h) Penconazol 20%
Figure 6:
Germinated and ungerminated spores of Beauveria
bassiana strain GHA

YEARBOOK 2009 · ADVANCES IN R&D
BESTCURE: Laboratory efficacy assays
against fungi and bacteria, compatibility
with biopesticides and control of Botrytis in
grapes in South Africa
BESTCURE is a liquid concentrate of citrus biomass and L-free amino
acids. The product is designed to activate the natural plant defenses
against attack of phytopathogenic fungi and bacteria. During 2009,
the R&D laboratory of FUTURECO BIOSCIENCE has been actively
working on providing information to support the handling of this
product. As a result, several in vitro tests have been carried out against
different pathogens and the compatibility with biopesticides has been
verified. In field conditions, BESTCURE was successfully tested to
suppress Botrytis in grapes.
Laboratory efficacy tests
A total of 14 assays were carried out during 2009 under laboratory
conditions. The target pathogens were fungus Fusarium oxysporum f.
sp. lycopersici and bacteria Xanthonomas spp, Ralstonia solanacearum,
Erwinia carotovorum and Pseudomonas syringae.
In 5 out the 6 tests on Fusarium oxysporum, BESTCURE showed to
be completely biocide (Table 1). The same effect was observed on
the three bacteria tested (Xanthonomas, Pseudomonas syringae and
Erwinia caractovora). In the 4 tests on Ralstonia solanacearum, the
total population was reduced between 30 and 50%.
F U T U R E C O B I O S C I E N C E
32

YEARBOOK 2009 · ADVANCES IN R&D
Table 1. Summary of the results from 14 in vitro assays against phytopathogen species with
BESTCURE (R&D Laboratory, Futureco Bioscience, 2009).
Fusarium oxysporum
Ralstonia solanacearum
Xanthomonas spp.
Compatibility with Biopesticides,
The compatibility of BESTCURE with two biopesticides (NOFLY and
BOTANIGARD) and one bionematicide was tested in three assays of
tank mixture simulation. A suspension of spores from each biopesticide
was mixed with three different concentrations of BESTCURE
(minimum, maximum and twice maximum commercial dose). Then
the germination of spores of Paecilomyces fumosoroseus (NOFLY),
Beauveria bassiana (BOTANIGARD) and Paecilomyces lilacinus after 0,
4 and 24 hours of contact was tested.
BESTCURE showed to be incompatible with NOFLY (Paecilomyces
fumosoroseus strain FE9901) and BOTANIGARD (Beauveria bassiana
strain GHA) and partially compatible with Paecilomyces lilacinus strain
PL0501.
Efficacy field trials
PATHOGEN ASSAY CONTROL BESTCURE 0.15% EFFECT
The efficacy of different products to control Botrytis cinerea in a
vineyard variety Thomson seedless in Elsenburg (Western Cape, South
Africa) was assessed. The objective of the study was to determine
the efficacy of each individual product and their combinations (1
fungicide, 2 botanicals (BESTCURE and other), 2 chemical references
and one untreated control) to suppress Botrytis in post-harvest.
The experiment consisted of 11 treatments distributed in 4 randomized
blocks with 5 plants per plot. In a first assay, 4 applications were made
1 1.51x10 7 0 100% biocide
2 3.65x10 7 5.00x10 5
3 9.80x10 7 0 100% biocide
4 9.75x10 7 0 100% biocide
5 5.45x10 7 0 100% biocide
6 1.36x108 0 100% biocide
1 2.11x1012 3.29x106 2 2.14x10 13 1.01x10 7
3 4.40x10 12 1.50x10 4
4 3.93x10 11 9.55x10 3
F U T U R E C O B I O S C I E N C E
33
30-50%
biocide
1 >1.00x10 11 0 100% biocide
2 >1.00x10 14 0 100% biocide
Pseudomonas syringae 1 1.70x10 8 0 100% biocide
Erwinia carotovora 1 3.80x10 9 0 100% biocide

at 4 critical moments of crop development: A: “flowering”; B: “pea
size” of fruits; C: “varaison” and D: “harvest” (at different days
according to label recommendations of each product). In the second
trial, 3 applications of the products were made (B, C and D).
The climatic conditions during the trial favored grape production
and also development of fungal pathogens. The daily temperatures
reached in both trials 35ºC, combined with low-medium relative
humidity. The night temperatures ranged between 8 and 20 ºC.
The infection level was determined from 10 randomly selected
branches in each plot (5 vineyards / plot). In both trials, a total of 5
evaluations were done: 5 days before, and 5, 9, 10 and 17 days after
the last application.
The preventive treatments were effective with few significant
differences among them. In the first trial, the efficacy of BESTCURE
treatments ranged from 94.7% and 98.4% while in the second trial
(3 applications), it was between 93.9% and 95.2% according to the
applied dose. No phytotoxicity symptoms were observed.
Table 2. Summary of results from two field trials on table grape variety “Thomson Seedless”
(Western Cape, Sudafrica)
Trt Product Rates
F U T U R E C O B I O S C I E N C E
34
TRIAL 1 TRIAL 2
Applications %bunch infection Applications %bunch infection
1 CONTROL (no treatment) ABCD 56.5 BCD 31.3
2 4 X Biofungicide 7L/ha ABCD 4.1 BCD 3.8
3 4 X Biofungicide 14L/ha ABCD 5.0 BCD 7.3
4 4 X Teldor 75 mL/100L agua ABCD 1.0 BCD 3.6
5
6
7
8
2 X Teldor +
2 X Biofungicide
75 mL/100L agua +
7L/ha
YEARBOOK 2009 · ADVANCES IN R&D
ABCD 3.9 BCD 7.4
4 X Biofungicide +
Nu-film P 7L/ha + 150mL/ha ABCD 0.9 BCD 6.1
4 X BotanicalFungicide DP
+ Sulfur 3kg/ha + 18kg/ha ABCD 1.9 BCD 6.1
4 X Fungicide Botánico DP
+ Sulfur 6kg/ha + 18kg/ha ABCD 0.8 BCD 2.9
9 4 X BESTCURE 1.5L/ha ABCD 0.9 BCD 1.9
10 4 X BESTCURE 3L/ha ABCD 3.0 BCD 1.5
11 4 X Sulfur 18kg/ha ABCD 1.0 BCD 3.5

YEARBOOK 2009 · ADVANCES IN R&D
F U T U R E C O B I O S C I E N C E
35

YEARBOOK 2009 · FAIRS AND CONGRESSES
7th New AG International
Conference in Barcelona
On the 25th to the 27th of March we held one of the most important
events in the High Tech Agriculture at the Princess Sofia Hotel in
Barcelona, the 7th New Ag International Conference. This conference
provides a framework to establish interesting business contacts and to
keep up to date on the latest advances in plant nutrition, application
of technology products, ferti-irrigation and crop production in
greenhouses .
The scientific program covered legal issues, technology development
and presentation of new products. Most conferences focused on
interest for low environmental impact technologies in agriculture
management as well as introduction of natural products in both
plant nutrition and plant protection. This fact was supported with the
presence of prestigious organizations such as International Biocontrol
Manufacturers Association.
FUTURECO BIOSCIENCE’s participation served as a meeting point
for our collaborators and the establishment of important contacts
with companies from Asia, Noth America, Latin America or Africa. It
was the perfect scenerio for the launch of FUTURECO BIOSCIENCE’s
new product catalogue. The catalogue´s innovative design allows to
permanently update the technical information for each product with
new results obtained by the Technical Department from laboratory
assays or field studies carried out.
The high number of commercial relationships held during the three
day conference was a step forward in the consolidation of the
internationalization of FUTURECO BIOSCIENCE.
F U T U R E C O B I O S C I E N C E
36
MARCH

YEARBOOK 2009 · FAIRS AND CONGRESSES
OCTOBER
4th Annual General
Meeting of IBMA in
Switzerland
FUTURECO BIOSCIENCE completed its fourth year in a row participating
in the Annual Biocontrol Industry Meeting of IBMA (International
Biocontrol Manufacturers Association http://www.ibma-global.org),
held every year at KKL international centre in Lucerne (Switzerland)
on the 19th and 20th of October. This meeting is jointly organized by
IBMA and the Institute of Research in Organic Agriculture (FiBL) and
supported by European Commission. ABIM-Lucerne is an excellent
event organized to fit the specific needs of biological control industry.
During the two intensive conference days, over 350 delegates
from 200 companies and organizations worldwide, got together
to exchange latest advances in Biological Control and availability of
commercial products.
The scientific program included 25 oral presentations covering the
following topics:
• New regulations affecting the European registration of plant
protection products based on biologicals
• Biocontrol industry and products situation in several markets:
Germany, Russia and North and South Africa
• Case study of successful experiences in the integration of
biological control products within IPM programs
• New products (biopesticides, beneficial insects, pheromones
and botanicals)
FUTURECO BIOSCIENCE was present with a stand that served as
meeting point for interaction with new and current customers. This
stand was one of the most visited and allowed the establishment of
potential new commercial agreements.
F U T U R E C O B I O S C I E N C E
37

YEARBOOK 2009 · FAIRS AND CONGRESSES
The second ONTA congress in Brazil
The 2nd International Congress of Tropical Nematology, a joint
meeting of the Organization of Nematologists of Tropical America
(ONTA) and Brazilian Society of Nematologists (BSN) was held in
Maceió, Brazil from the 4th to the 9th of October 2009. Over 250
participants from different countries attended the congress. The 32
scientific sessions presented 136 oral conferences and 300 posters,
covering a wide range of issues ranging from the economical impact
of plant parasitic nematodes to pest management in different crops,
chemical and biological control strategies, biofumigation, rotation,
new molecular techniques for identification, use of entomopathogenic
nematodes, interactions plant-nematodes-soil microorganisms, and
soil biodiversity.
FUTURECO BIOSCIENCE presented two posters in that congress which
summarized two research projects:
• Development of a nematicide extract derived from fermentation
broth of Paecilomyces lilacinus
• Evaluation and Selection of Biological control agents (a project
funded by the Catalonian Government (CIDEM))
FUTURECO BIOSCIENCE is strongly focused in the research of effective
and environmentally friendly tools for the management of plant
parasitic nematodes. In consequence, the research and development
department is currently developing three “bio-nematicides” based on
diverse microbial-based active substances: bacteria cells, fungal spores
and secondary metabolites extract of fermentation broths. Until
now, the results in small plots are promissory, and despite the lack
of toxicological, ecotoxicological and stability studies of formulations,
the trend of this work lead to three new commercial biological
nematicides for the benefit of both farmers and agriculture.
F U T U R E C O B I O S C I E N C E
38
OCTOBER

YEARBOOK 2009 · FAIRS AND CONGRESSES
DECEMBER
Second symposium
on formulation of
microorganisms
The DOM research programme - Domestication of Microorganisms
- aims to decrease the use of chemicals in the environment, by
generating new knowledge about use of special properties of
naturally occurring microorganisms. Within this project, the second
International Symposium on Microbial Formulation was arranged at
Campus Ultuna of the Swedish University of Agricultural Sciences in
Uppsala on December 1-2.
This symposium was directed to the community of researchers and
industrials dealing with various aspects of microbial formulation and
stabilization of living microorganisms in areas such as food, agriculture,
environment, biotechnology, pro-biotics and biomedicine. A total of
21 oral presentations and 10 posters covered different aspects of
microbial formulation and presented some of the latest advances of
research groups involved in the DOM project. The event also included
keynote conferences from four prominent speakers. The presentation
“Formulating mycoinsecticides: science, art and alchemy” by Dr
Stefan Jaronski from the U.S. Department of Agriculture, was the
most informative for the in-house research programs of FUTURECO
BIOSCIENCE.
The major goal of the symposium was to promote interdisciplinary
collaboration and to establish fruitful exchange of ideas and contacts
between academia and industry from multiple domains. Moreover,
the symposium addressed the potential of microbial formulation, its
materials and technology.
The presence of FUTURECO BIOSCIENCE was within the framework
of development of new products based on naturally beneficial
microorganisms occurring for both plant protection and plant
nutrition.
F U T U R E C O B I O S C I E N C E
39

FUTURECO BIOSCIENCE S.L.
www.futurecobioscience.com