B. D. Bowes, H. Koku, A. M. Lenhoff, Characterization of Protein Transport and Adsorption in Polymer-Modified Ion- Exchange Media, oral presentation, PREP Symposium, San Jose, CA, June 2008. H. Koku, B. D. Bowes, A. M. Lenhoff, Microstructure and Retention Properties of Dextran-Grafted Agarose Media, oral presentation, 234 th ACS National Meeting, BIOT Division, Boston, MA, August 2007. LEADERSHIP & VOLUNTEER EXPERIENCES Madison House Volunteer, University of Virginia • Helped build energy-efficient houses for low income families Eagle Scout of America • Led a project to plant shade trees at a local school
Zachary T. Britton Business Address: 150 Academy Street Current Address: 316 North Barrett Lane Colburn Laboratory, Room 002 Newark, DE 19702 Newark, De 19702 302-379-8815 (cell) 302-831-6697 zbritton1122@hotmail.com ztb@udel.edu (U.S. Citizen) QUALIFICATIONS PhD in <strong>Chemical</strong>/Biochemical <strong>Engineer</strong>ing (Expected May 2011) Multi-disciplinary training and skill set with expertise in protein biochemistry and protein engineering, including molecular biology, protein expression, purification and characterization. Strong communication skills and demonstrated ability to design, execute and analyze experiments independently. Effective as a leader and team player in a collaborative environment. EDUCATION University of Delaware, Newark, Delaware GPA: 3.52 / 4.00 PhD Candidate <strong>Chemical</strong> <strong>Engineer</strong>ing (May 2011) Biochemical <strong>Engineer</strong>ing and Biotechnology Focus Advisor: Anne S. Robinson Carnegie Mellon University, Pittsburgh, Pennsylvania GPA: 3.63 / 4.00 BS <strong>Chemical</strong> <strong>Engineer</strong>ing and Biomedical <strong>Engineer</strong>ing, Chemistry Minor May 2004 University and College Honors HONORS AND Addgene Recombinant DNA Technology Award, October 2010. AWARDS National Institutes of Health Chemistry-Biology Interface (CBI) Training Fellowship, 2004 – 2006. Biomedical <strong>Engineer</strong>ing Society Undergraduate Student Travel Award, October 2003. SELECTED Biomolecular and Biochemical <strong>Engineer</strong>ing Laboratory, University of Delaware, Newark, DE RESEARCH Graduate Student <strong>Research</strong>er, October 2006-Present EXPERIENCE – Characterized protein and cellular limitations to functional production of G protein-coupled receptors (GPCRs) in S. cerevisiae. − Pioneered efficient in vivo cloning methods for protein engineering applications, including generation of fusion proteins, receptor chimeras and mutagenesis libraries. − Purified human adenosine receptors from S. cerevisiae using immobilized-ligand affinity chromatography. − Designed a novel expression and purification system for the production of transmembrane peptides of GPCRs in E. coli. Cardiovascular and Cancer Biology Laboratory, Ulhas Naik, University of Delaware, Newark, DE Graduate Student <strong>Research</strong>er, April 2005-October 2006 − Studied the phosphatase activity and regulation of calcium and integrin binding protein 1 (CIB1). Musculoskeletal <strong>Research</strong> Center, James H-C Wang, University of Pittsburgh, Pittsburgh, PA Undergraduate <strong>Research</strong>er, June 2003- Spring 2005 − Characterized the differences between rat MCL fibroblasts from wounded and control groups. Bone Tissue <strong>Engineer</strong>ing Center, Jeffrey Hollinger, Carnegie Mellon University, Pittsburgh, PA Undergraduate <strong>Research</strong>er, January 2003-May 2004 − Synthesized, purified and analyzed novel polymers for use in hydrogels for DNA delivery. TEACHING Teaching Assistant, Biochemical <strong>Engineer</strong>ing, University of Delaware, Newark, DE, Fall 2007; EXPERIENCE Instructors: Maciek R. Antoniewicz and Millicent O. Sullivan − Independently lead two lectures, directed review sessions, and graded homework and projects. <strong>Research</strong> Mentor, University of Delaware, Newark, DE, Spring 2009 - Fall 2010. − Trained a post-doctoral fellow in bacterial culture and phage-display. − Trained and coordinated three undergraduate students in yeast and bacterial methods for GPCR production and purification. − Trained a graduate student in protein expression and optimized γ-D-crystallin in E. coli. SKILLS <strong>Research</strong>: Eukaryotic and Prokaryotic Cell Culture (Mammalian, Insect cell, Yeast, and Bacteria) Protein and Biochemistry Methods (Protein Expression, Chromatographic Separations, Circular Dichroism, Intrinsic Tryptophan Fluorescence, SDS-PAGE, Western blotting) Organic Synthesis and Separations Techniques Radio-labeling Techniques ( 3 H, 35 S, 32 P) 1/2
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