Research /Process Chemical Engineer - Chemical Engineering ...

OBJECTIVE
ANUP AGARWAL
334 East Main Street, Apt. D10, Newark, DE 19711
Ph: 302-690-9045. E-mail: anup@udel.edu
A full time position in research or development in a biotechnology or a pharmaceutical company that best utilizes my
experimental and technical skills.
RESEARCH EXPERIENCE
Graduate Research Assistant November 2008 – present
Computational and experimental approaches to enhance the extracellular secretion of recombinant proteins in
Escherichia coli
• Optimized the extracellular levels of a model protein by developing alternative kinetic models in MATLAB.
• Enhanced recombinant protein secretion by using targeted site directed mutagenesis, genomic knockout and
standard molecular cloning and expression techniques.
Advisor: Prof. Kelvin H. Lee, Chemical Engineering, University of Delaware, Newark, DE, USA.
Undergraduate Dissertation August 2007 – May 2008
Optimization of process parameters for the production of L-asparaginase from a newly isolated Serratia marcescens
• Designed experiments by applying response surface methodology using the Minitab statistical software to optimize
the production of L-asparaginase in shake flasks.
• Scaled the production of L-asparaginase in batch bioreactors.
• Analyzed the effect of physiological conditions to enhance the production of L-asparaginase in batch bioreactors.
Advisor: Prof. V. Venkata Dasu, Biotechnology, IIT Guwahati, Asom, India.
Invited Research Intern May 2007 – July 2007
Design and analysis of bioprocesses for the manufacture of a functional peptide at commodity scale
• Developed and analyzed alternative process flow-sheets for cost and performance using the SuperPro Designer
simulation package.
Advisor: Prof. Anton Middelberg, Biomolecular Engineering, University of Queensland, Brisbane, Australia.
Scholarship Intern May 2006 – December 2006
Kinetic modeling of quorum sensing system in bacteria
• Developed and extended kinetic models of quorum sensing system in bacteria using MATLAB and Mathematica.
Advisor: Prof. Sarika Mehra, Chemical Engineering, IIT Bombay, Mumbai, India.
PUBLICATIONS
• Agarwal A, Kumar S, Veeranki VD. Effect of carbon and nitrogen sources, pH and dissolved oxygen level on the
production of L-asparaginase from a newly isolated Serratia marcescens SK-07. Letters in Applied Microbiology.
Submitted 2010.
• Agarwal A, Lee KH. Optimization of extracellular secretion of recombinant proteins via a Type-I pathway in
Escherichia coli using kinetic modeling approach. In preparation.
EDUCATION
Master of Chemical Engineering, University of Delaware, Newark, DE, USA Expected December 2010
Cumulative GPA: 3.83/4.0
Bachelor of Technology, Biotechnology, Indian Institute of Technology (IIT) Guwahati, Asom, India May 2008
Cumulative GPA: 8.79/10.0 (Rank: 4)
HONORS AND ACTIVITIES
• Qualified amongst top 1% of 0.2 million students in IIT-Joint Entrance Exam to join IIT Guwahati (2004).
• Selected for the IIT Bombay Summer Intern Scholarship (2006).
• Merit cum Means Scholarship at IIT Guwahati for academic excellence (2005 – 2008).
• Leader of a team of 10 people responsible for publicity and marketing of the annual festival of IIT Guwahati (2007).
• Member and lead actor, dramatics team of the annual intra-IIT cultural festival of IIT Guwahati (2004 – 2005).