Towards clinico-pathological application of Raman spectroscopy

76 Chapter 4
(Size:28 x 24µm)
Figure 7.
A
B
Intensity (a.u.)
400 600 800 1000 1200 1400 1600 1800
Raman shift (cm –1 )
Figure 7. A: Photomicrograph of unstained frozen section showing a necrotic area of glioblastoma with a
microscopic crystal-like inclusion (original magnification, x80). B: Raman map (upon KCA) of the histological
detail displayed in A (scanning step size 2 μm). C: Raman spectrum (average) of the crystal-like inclusion
(predominantly cholesterol, see Figure 3E).
(Size: 15.5 x 10.5 �m)
c
b
a
A
B
Intensity (a.u.)
C
c
C
400 600 800 1000 1200 1400 1600 1800
Raman shift (cm -1 )
Figure 8. A: Bright-field image of calcification deposit in a necrotic glioblastoma region (original
magnification, x80). B: Raman map, based on the KCA with 3 clusters (scanning step size 0.5 μm). C:
Raman spectra (averages) of (a) surrounding necrotic tissue, (b) periphery of calcification, and (c) center of
calcification (predominantly calcium hydroxyapatite, see Figure 3F).
Figure 8.
a
b