MICROBIOLOGY - OASIS-lab

Anhydrous Nutrient Media for Molecular Biology
Anhydrous Nutrient Media for Assays
in Molecular Biology
Type Art. No. Pack Qty. €
X968.1 500 g 42,90
LB Broth (Luria/Miller)
X968.2
X968.3
1 kg
2.5 kg
79,55
174,15
X968.4 5 kg 311,75
X969.1 500 g 45,05
LB Agar (Luria/Miller)
X969.2 1 kg 83,85
X969.3 2.5 kg 188,15
X964.1 500 g 47,90
LB Broth (Lennox)
X964.2
X964.3
1 kg
2.5 kg
85,90
195,65
X964.4 5 kg 333,25
X965.1 500 g 52,90
LB Agar (Lennox)
X965.2 1 kg 100,20
X965.2 2.5 kg 239,75
NZCYM Broth X974.1 500 g 47,85
NZY Broth X973.1 500 g 50,65
SOB Broth AE27.1 500 g 55,65
Terrific Broth
X972.1
X972.2
500 g
1 kg
53,55
95,70
Terrific-Broth modified
HP61.1
HP61.2
500 g
1 kg
53,65
95,70
Yeast-Nitrogen-Base (without Amino Acids) HP26.1 500 g 121,50
YPD Broth X970.1 500 g 49,60
YPD Agar X971.1 500 g 62,35
X966.1 500 g 54,95
2x YT Broth
X966.2 1 kg 96,25
X966.3 2.5 kg 225,75
2x YT Agar X967.1 500 g 59,90
* Additional data, safety information, and further package sizes, see our current catalogue,
chapter Chemicals A-Z, or online at www.carlroth.com
α-D(+)-Glucose monohydrate
for molecular biology
Biochemical and microbiological applications.
Dextrose, blood sugar
C 6H 12O 6 · H 2O
M 198,17 g/mol
8
6887.1 1 kg plastic 22,20 €
6887.2 2,5 kg plastic 44,45 €
6887.5 25 kg plastic 137,60 €
SOB
Super Optimal Broth (SOB) is a complex, nutritious nutrient medium which is used prior
to and after transformation of E. coli bacteria. By adding 20 mM glucose it is enriched to
SOC (Super Optimal Broth with Catabolite repression) which is optimal for coddling up of
freshly transformed bacteria. Through this, SOC increases transformation efficiency.
In order to avoid caramelisation, glucose may only be added after autoclaving.
Acc. to D. Hanahan (1983) J. Mol. Biol. 166/4:557-580
Roti ® -Transform
Sterile. For preparation of competent E. coli.
Store at -20 °C, Transport temp.: room temp.
• Flexible selection of strains
• Effective, simple and fast
• Adequate for 500 transformations
Roti ® -Transform
Roti ® -Transform is a simple, effective and fast method of producing
competent E. coli in three steps. The competent cells may be used
directly for transformation or may be frozen for later transformations.
Transformation efficiency is 5 x 10 5 to 1 x 10 6 colonies per µg DNA.
Long-term Storage of the Kit at -20 °C.
Storage for use:
Reagent 1: Short-term storage may be done at 4 °C. For long-term storage
of over 14 days we recommend a storage temperature of -20 °C.
Reagent 2: Storage at 4 °C.
Roti ® -Transform consists of 45 ml Roti ® -Transform 1 (Art. No. P041) and
5 ml Roti ® -Transform 2 (Art. No. P042). One kit is sufficient for producing
competent E. coli for 500 transformations.
Contents of this Kit may not be bought separately.
P043.1 1 kit 53,70 €
Standard Code Designations
S re
Abbreviation Broth/Agar Art. No.
CASO CASO Agar or Broth X924, X925
CLED CLED Agar 2835
CN Pseudomonas Selective Agar CP67
DG18 Dichlorane Glycerol Agar AE26
DRBC Dichlorane Bengal Red Agar AE25
DRCM Differential-Reinforced Clostridia Medium CL78
mCP m-CP Agar CL55
MRS MRS Agar or Broth X924, X925
PC Plate Count Agar X930
R2A R2A Agar CL01
RBC Bengal Red Agar AE24
RCM Reinforced Clostridial Medium AE66
RVS Rappaport Vassiliadis Broth CL03
TBA Tryptone Bile Salts Agar CP69
TBX TBX Chromo Agar CL47
TSA Trypton Soy Agar CP70
TSB CASO Broth X938
TSB-Agar CASO Agar X937
TSC TSC Agar CL51
VRBD Violet Red Bile Agar with Glucose X939
VRB Violet Red Bile Agar with Lactose X940
XLD XLD Agar X941
YGC Yeast Glucose Agar CL00
YNB Yeast Nitrogen Base HP26
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