882014-2-Solution DAB Kit - Invitrogen

88-2014 1 kit
2-Solution DAB Kit (1000 slides)
Lot No.
2-Solution DAB Kit
INTRODUCTION
This DAB (diaminobenzidine) substrate will produce a brown colored deposit upon
reaction with HRP (horseradish peroxidase), and is intended to be used in
immunohistochemistry protocols. This DAB product has improved stability, ease of
formulation, and greater sensitivity than previous Zymed DAB products.
REAGENTS PROVIDED
A. One bottle (110 ml) DAB Substrate Buffer (Ready-To-Use)
B. One dropper bottle (3.5 ml) DAB Chromogen (40X)
PROTOCOL
1. Add 1 drop (~26.5 µl) of DAB Chromogen (Reagent B) to 1 mL of DAB
Substrate Buffer (Reagent A). Mix well.
2. Add to tissue specimen and develop for 5 min.
3. Rinse for 2 minutes with running tap water.
4. Proceed with normal counterstaining and mounting protocols.
RECOMMENDED GUIDELINES FOR USE
1. Signal development may be monitored under a microscope.
2. Keep solution away from light and use within 2 hours.
STORAGE
Store at 2-8°C
WARNING:
DAB is a possible carcinogen. Please take necessary precautions.
Authorized Representative for IVDD 98/79/EC: Invitrogen Ltd.
Inchinnan Business Park
3 Fountain Drive
Paisley
PA4 9RF
UK
"General Purpose Reagent. For Laboratory Use"
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288
E-mail: techsupport@invitrogen.com
PI 882014 (Rev 11/08) DCC-08-1895
© 2007 Invitrogen Corporation. All right reserved. These products may be covered by one or more Limited Use Label
Licenses (see the Invitrogen Catalog or www.invitrogen.com). By use of these products you accept the terms and
conditions of all applicable Limited Use Label Licenses.
88-2014 1 kit
2-Solution DAB Kit (1000 slides)
Lot No.
2-Solution DAB Kit
INTRODUCTION
This DAB (diaminobenzidine) substrate will produce a brown colored deposit upon
reaction with HRP (horseradish peroxidase), and is intended to be used in
immunohistochemistry protocols. This DAB product has improved stability, ease of
formulation, and greater sensitivity than previous Zymed DAB products.
REAGENTS PROVIDED
A. One bottle (110 ml) DAB Substrate Buffer (Ready-To-Use)
B. One dropper bottle (3.5 ml) DAB Chromogen (40X)
PROTOCOL
1. Add 1 drop (~26.5 µl) of DAB Chromogen (Reagent B) to 1 mL of DAB
Substrate Buffer (Reagent A). Mix well.
2. Add to tissue specimen and develop for 5 min.
3. Rinse for 2 minutes with running tap water.
4. Proceed with normal counterstaining and mounting protocols.
RECOMMENDED GUIDELINES FOR USE
1. Signal development may be monitored under a microscope.
2. Keep solution away from light and use within 2 hours.
STORAGE
Store at 2-8°C
WARNING:
DAB is a possible carcinogen. Please take necessary precautions.
Authorized Representative for IVDD 98/79/EC: Invitrogen Ltd.
Inchinnan Business Park
3 Fountain Drive
Paisley
PA4 9RF
UK
"General Purpose Reagent. For Laboratory Use"
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288
E-mail: techsupport@invitrogen.com
PI 882014 (Rev 11/08) DCC-08-1895
© 2007 Invitrogen Corporation. All right reserved. These products may be covered by one or more Limited Use Label
Licenses (see the Invitrogen Catalog or www.invitrogen.com). By use of these products you accept the terms and
conditions of all applicable Limited Use Label Licenses.

88-2014 1 kit
2-Solution DAB Kit (1000 portaobjetos)
2-Solution DAB Kit
INTRODUCCIÓN
Este sustrato producirá un depósito de color marrón al reaccionar con la peroxidasa.
REACTIVOS SUMINISTRADOS
A. 1 frasco (110 ml) de DAB Substrate Buffer (listo para usar)
B. 1 frasco cuentagotas (3.5 ml) de DAB Chromogen (concentración 40x)
PROTOCOLO
1. Añadir una gota (~26.5 µL) de DAB Chromogen (Reactivo B) a 1 ml de DAB
Substrate Buffer (Reactivo A). Mezclar bien.
2. Incubar la mezcla de reactivos durante unos 5 minutos a temperatura
ambiente.
3. Enjuagar por 2 minutos con el agua del grifo.
4. A partir de este punto, el investigador debe proceder siguiendo sus propios
protocolos de laboratorio para la contratinción y el montaje.
DIRECTRICES DE USO RECOMENDADAS
1. La evolución del color puede observarse con el microscopio.
2. Mantener la solución apartada de la luz. No dejar pasar más de 2 horas
antes de su utilización.
ALMACENAMIENTO
Guardar a una temperatura de entre 2ºC y 8ºC.
ADVERTENCIA:
El DAB es un carcinógeno posible. Tomar las medidas de prevención necesarias.
Representante autorizado de IVDD 98/79/EC: Invitrogen Ltd.
Inchinnan Business Park
3 Fountain Drive
Paisley
PA4 9RF
UK
"General Purpose Reagent. For Laboratory Use"
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288
E-mail: techsupport@invitrogen.com
PI 882014 (Rev 11/08) DCC-08-1895
© 2007 Invitrogen Corporation. All right reserved. These products may be covered by one or more Limited Use Label
Licenses (see the Invitrogen Catalog or www.invitrogen.com). By use of these products you accept the terms and
conditions of all applicable Limited Use Label Licenses.
88-2014 1 kit
2-Solution DAB Kit (1000 lames)
2-Solution DAB Kit
INTRODUCTION
Ce substrat produira un dépôt de couleur marron à la réaction du peroxydase.
REACTIFS FOURNIS
A. Une bouteille (110 ml) de DAB Substrate Buffer (prêt à l'utilisation)
B. Une bouteille compte-gouttes (3.5 ml) de DAB Chromogen (40X)
PROTOCOLE
1. Ajouter 1 goutte (~26.5 µl) du DAB Chromogen (Réactif B) ΰ 1 ml de DAB
Substrate Buffer (Réactif A). Bien mιlanger.
2. Incuber le mélange réactif pour ~5 minutes à température ambiante.
3. Rincer pendant 2 minutes de l'eau du robinet.
4. À partir d’ici, l’investigateur doit procéder avec ses propres protocoles de
laboratoire pour la contre-coloration et la fixation.
DIRECTIVES D'UTILISATION RECOMMANDEES
1. Le développement des couleurs peut être régulé au microscope.
2. Garder la solution loin de la lumière et utiliser la dans les 2 heures qui
suivent.
STOCKAGE
Garder à 2-8°C.
AVERTISSEMENT:
DAB peut être carcinogène. Merci de prendre les précautions nécessaires.
Représentant Autorisé pour IVDD 98/79/EC: Invitrogen Ltd.
Inchinnan Business Park
3 Fountain Drive
Paisley
PA4 9RF
UK
"General Purpose Reagent. For Laboratory Use"
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288
E-mail: techsupport@invitrogen.com
PI 882014 (Rev 11/08) DCC-08-1895
© 2007 Invitrogen Corporation. All right reserved. These products may be covered by one or more Limited Use Label
Licenses (see the Invitrogen Catalog or www.invitrogen.com). By use of these products you accept the terms and
conditions of all applicable Limited Use Label Licenses.

88-2014 1 corredo
2-Solution DAB Kit (1000 vetrini)
2-Solution DAB Kit
INTRODUZIONE
Questo substrato produrrà un deposito di colore marrone in reazione con perossidasi.
REAGENTI FORNITI
A. Una boccetta (110 ml) di DAB Substrate Buffer (pronto per l’uso)
B. Una boccetta contagocce (3.5 ml) di DAB Chromogen (40X)
PROTOCOLLO
1. Aggiungere 1 goccia (~26.5 µl) di DAB Chromogen (Reagente B) ad 1 ml di
DAB Substrate Buffer (Reagente A). Mescolare bene.
2. Incubare la mistura di reagenti per ~5 minuti a temperatura ambiente.
3. Risciacquare con 2 minuti di acque di rubinetto.
4. Da questo punto in poi, l’investigatore dovrebbe attenersi ai protocolli vigenti
presso il proprio laboratorio per l’esecuzione delle procedure di
controcolorazione e fissaggio.
RACCOMANDAZIONI PER L’USO
1. Lo sviluppo del colore può essere monitorato al microscopio.
2. Conservare la soluzione al riparo dalla luce ed utilizzare entro 2 ore.
CONSERVAZIONE
Conservare a 2-8 °C
AVVERTENZA
DAB è considerata un sospetto carcinogeno. Seguire dunque tutti gli accorgimenti
raccomandati per le sostanze potenzialmente carcinogene.
Rappresentante Autorizzato per IVDD 98/79/EC: Invitrogen Ltd.
Inchinnan Business Park
3 Fountain Drive
Paisley
PA4 9RF
UK
"General Purpose Reagent. For Laboratory Use"
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288
E-mail: techsupport@invitrogen.com
PI 882014 (Rev 11/08) DCC-08-1895
© 2007 Invitrogen Corporation. All right reserved. These products may be covered by one or more Limited Use Label
Licenses (see the Invitrogen Catalog or www.invitrogen.com). By use of these products you accept the terms and
conditions of all applicable Limited Use Label Licenses.
88-2014 1 Installationssatz
2-Solution DAB Kit (1000 Objektträger)
2-Solution DAB Kit
EINFÜHRUNG
Dieses Substrat wird mit der Reaktion auf Peroxidase eine rot gefärbte Ablagerung
erzeugen.
GELIEFERTE REAGENTIEN
A. Eine Tropfflasche (110 ml) DAB Substrate Buffer (gebrauchsfertiger)
B. Eine Tropfflasche (3.5 ml) DAB Chromogen (40X)
PROTOKOLL
1. 1 Tropfen (~26.5 µl) DAB Chromogen (Reagens B) auf 1 ml DAB Sustrate
Buffer (Reagens A). Gut mischen.
2. Die Reagensmischung für 5 Minuten bei Zimmertemperatur inkubieren.
3. 2 Minuten in Leitungswaßer.
4. Ab diesem Punkt sollte der Prüfer gemäß den eigenen festgelegten
Laborprotokollen für Gegenfärbung und Mounting fortfahren.
EMPFOHLENE BENUTZUNGSRICHTLINIEN
1. Farbentwicklung kann unter einem Mikroskop überwacht werden.
2. Die Lösung fern von Licht halten und sie innerhalb 2 Stunden benutzen.
LAGERUNG
Bei 2-8°C lagern
WARNUNG:
DAB ist ein mögliches karzinogen. Bitte unternehmen sie die notwendigen
Vorsichtsmassnahmen.
Bevollmächtigter Repräsentant für IVDD 98/79/EC: Invitrogen Ltd.
Inchinnan Business Park
3 Fountain Drive
Paisley
PA4 9RF
UK
"General Purpose Reagent. For Laboratory Use"
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288
E-mail: techsupport@invitrogen.com
PI 882014 (Rev 11/08) DCC-08-1895
© 2007 Invitrogen Corporation. All right reserved. These products may be covered by one or more Limited Use Label
Licenses (see the Invitrogen Catalog or www.invitrogen.com). By use of these products you accept the terms and
conditions of all applicable Limited Use Label Licenses.