Parasite-host relationship: a lesson from a professional killer
Parasite-host relationship: a lesson from a professional killer
Parasite-host relationship: a lesson from a professional killer
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Fig. 6 To identify putative specific parasite-bound HIPs, high-salt elutions <strong>from</strong> parasite body surface (previously<br />
incubated with <strong>host</strong> hemolymph) were carried out. The supernatants of the elution were analyzed by SDS–PAGE<br />
(panel B). The parasites were thoroughly washed to eliminate all non-specific hemolymph proteins before elution.<br />
The electrophoretic pattern (panel B, lane “elu”) revealed the presence of four main bands with molecular masses<br />
of about 80, 35, 26, and 17 kD, respectively. When the assay was carried out with methanol–chloroform or lipasetreated<br />
parasites, the lower bands (35, 26, and 17 kD) disappeared (panel B, lanes “MC” and “Lip”) and only a<br />
reduced amount of the 80 kD band was observed by SDS–PAGE. Panel C: further interaction assays were<br />
carried out with hemolymph (
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