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PhD Thesis MJProl .pdf - digital-csic Digital CSIC - Consejo Superior ...

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xiv Index<br />

6.3.1. Culture of Phaeobacter 27-4 on different supports and<br />

conditions 122<br />

6.3.2. In vitro inactivation and inhibition of Listonella anguillarum 90-<br />

11-287 and Vibrio splendidus DMC-1 by Phaeobacter 27-4<br />

biofilters 125<br />

6.3.2.1. Inactivation 125<br />

6.3.2.2. Inhibition 128<br />

6.3.3. Residence time of Phaeobacter 27-4 in biofilters maintained<br />

under turbot larval rearing conditions 130<br />

6.3.3.1. Analysis of DGGE profiles 133<br />

6.3.4. Effect of matured Phaeobacter 27-4 biofilters against Listonella<br />

anguillarum 90-11-287 maintained under turbot larval rearing<br />

conditions 134<br />

6.3.5. In vivo probiotic effect of Phaeobacter 27-4 biofilters on turbot<br />

larvae 136<br />

6.3.5.1. Analysis of DGGE profiles 139<br />

6.4. DISCUSSION 145<br />

6.4.1. Culture of Phaeobacter 27-4 on different supports and<br />

conditions 147<br />

6.4.2. In vitro inactivation and inhibition of Listonella anguillarum 90-<br />

11-287 and Vibrio splendidus DMC-1 by Phaeobacter 27-4<br />

biofilters 148<br />

6.4.3. Permanence of Phaeobacter 27-4 in biofilters and effect of<br />

matured biofilters against Listonella anguillarum 90-11-287<br />

maintained under turbot larval rearing conditions 149<br />

6.4.4. In vivo probiotic effect of Phaeobacter 27-4 biofilters on turbot<br />

larvae 150<br />

6.4.4.1. Influence of Phaeobacter 27-4 biofilters on microbial<br />

community of turbot larvae 153<br />

6.5. CONCLUSIONS 154<br />

6.6. REFERENCES 155<br />

7. FINAL DISCUSSION, CONCLUSIONS AND FUTURE<br />

PERSPECTIVES 163<br />

7.1. FINAL DISCUSSION 163<br />

7.2. CONCLUSIONS 168<br />

7.3. FUTURE PERSPECTIVES 171

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