Nucleic Acid Isolation.indd - Invitrogen

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Isolate mRNA from Any Sample Mammals Species Starting Sample - Specific Tissue or Cell Type Bovine Corpus luteum Dog Leucocytes Gerbil Cochlear cells Guinea pig Organ of corti (inner ear), Spiral ganglion (inner ear) Human Mouse Pig Adipose tissue, Heart Rat Birds Pigeon Breast muscle, Frozen tissues, Liver Fish Amniocytes, B cells, Blastocysts, Blood, Bone marrow, Brain, Breast, Bronchoalveolar cells, Cartilage, CD34 + cells, Cervical cancer cells, Chondrocytes, Colon carcinoma cells, Cultured cells, Embryo, Fibroblasts, Glomeruli (kidney), Gut, Hair roots, Heart, Hepatocytes, Keratinocytes, Kidney, Langerhans cells, Leucocytes, Leukemia cells, Liver, Lung, Lymphoblasts, Lymphocytes, Lymphoid cells, Monocytes, Muscles, Neutrophils, Oocytes, Pancreas, Peripheral blood mononuclear cells (PBMC), Peritoneal exudate cells, Placenta, Platelets, Retina, Skin, Stem cells, T cells, Testicles, Tumours, Umbilical vein endothelial cells Brain, Cortex, Embryonic tissue, Epidermis, Eye, Fibroblasts, Germ cells, Glomeruli (kidney), Heart, Interscapular brown adipose tissue pad, Kidney, Kidney tubules, Liver, Lung, Retina, Skeletal muscle, Testicles Adrenals, Brain, Cerebral cortex (pre-optic area), Heart, Hypothalamus (brain), Kidney, Liver, Lung, Muscle, Neuronal cells (brain), Organ of corti (inner ear), Pancreas, Pituitary, Renal cortex (kidney), Spiral ganglion (inner ear), Spleen Trout Brain, Eggs, Fibroblasts, Liver, Muscle, Ovaries, Pronephros Zebrafish Embryo Amphibians Xenopus laevis Liver, Ovaries Molluscs Sea Urchin Blastocysts, Coelomocytes, Eggs Insects Drosophila melanogaster Ovary, Whole insects Plants Yeasts Parasites Viruses Arabidopsis thaliana Hordeum vulgare Brassica oleracea Leaves, Root, Stigma Lupinus angustifolius Cotyledon Flowers, Leaves, Roots, Rosette leaves, Seed, Silique, Stem, Stem leaves, Whole plants Embryo, Endosperm , Leaves, Root, Seed aleurone, Seed embryos, Seed endosperm, Seed Zea mays Embryos, Endosperm, Flowers, Leaves, Ovules, Root, Seed Nicotiana tabacum Bud, Leaf (nuclei), Leaves, Root (nuclei), Seed, Stem Pisum sativum Root apical meristems (nuclei) Solanum tuberosum Leaves, Stolon tips Picea ssp. Root Lycopersicon ssp. Candida tropicalis Cell culture Hansenula polymorpha Soil samples Saccharomyces cerevisiae Cell culture, Soil samples Cryptosporidium parvum Oocyst Schistosoma mansoni Whole worm HCMV Infected cells HIV-1 HIV-2 Plasma HTLV-I/II Infected cells Human coronavirus Infected cells Poliovirus Infected cells Epidermal leaf cell (single cells), Flower bud, Flowers, Guard cell in leaf (single cells), Leaves Cells in broncho-alveolar washes, Cerebrospinal fluid, PBMC, Plasma, Serum, T lymphocytes cell line, CD4+ cells Table 2. An overview of some of the many sample types from which mRNA has been isolated using Dynabeads ® Oligo (dT) 25 . All information from published literature. For a full reference-list, please contact Dynal Biotech Technical Support. 72 www.dynalbiotech.com
Direct mRNA isolation with the Dynabeads ® mRNA DIRECT Kit* (Product no. 610.11/12) takes only 15 minutes from start to finish. The starting sample can be crude extracts of animal tissues, plants and cells. This new and improved kit contains 5 times more Dynabeads ® Oligo(dT) 25 . The protocol and kit format of the Dynabeads ® mRNA DIRECT Micro Kit (Product no. 610.21) is adjusted to small sample requirements, providing a fast and reliable way to combine direct mRNA isolation followed by direct RT-PCR amplification. The mRNA can alternatively be purified from total RNA with the Dynabeads ® mRNA Purification Kit (Product no. 610.06). A typical mammalian cell contains about 10 pg of total RNA, of which 1-5 % is mRNA. Dynabeads ® Oligo(dT) 25 (Product No. 610.02/05/50) is the main component in all of the above mentioned kits and is also available as a stand-alone product. The ease and speed of magnetic isolation and handling of mRNA provide a superior alternative to traditional methods. Isolate mRNA from Any Sample! As documented in a large number of scientific publications, Dynabeads ® Oligo(dT) 25 have been used to isolate mRNA from virtually any cell or tissue sample. Effective cell lysis and rapid handling make it possible to isolate mRNA from tough samples such as yeast cells, plant material with high starch and/or RNase content, paraffin-embedded tissues and even directly from human whole blood. The table on the facing page (table 2), lists some of the many different sample types where Dynabeads ® Oligo(dT) 25 have been used to isolate mRNA directly from a crude starting sample (all information from published literature). www.dynalbiotech.com 73 Choose the Right Product New & improved Fig. 2: The true uniform size and shape of Dynabeads ® Oligo (dT) 25 provide rapid and efficient binding kinetics and highly reproducible results. 6 7 Nucleic Acid Isolation
Page 1 and 2: 6. Nucleic Acid Isolation Isolation
Page 3: Nucleic Acid Isolation Isolation of
Page 7 and 8: mRNA for cDNA Libraries Dynabeads
Page 9 and 10: Ordering Information www.dynalbiote
Page 11 and 12: A Variety of Sample Types and Downs
Page 13 and 14: Dynabeads ® Streptavidin for Super
Page 15 and 16: Isolating RNA and DNA Binding Prote
Page 17 and 18: Dye Terminator Removal Removal of u

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