Thermo Scientific Phusion Flash High-Fidelity PCR Master Mix ...

6. Component specifications
6.1 Phusion Flash II Dna Polymerase
Thermostable Phusion DNA Polymerases are purified from recombinant
E. coli strains. Phusion DNA Polymerases possess the following
activities: 5’→3’ DNA polymerase activity and 3’→5’ exonuclease
activity. The Affibody ligand is purified from an E. coli strain carrying
a plasmid encoding Affibody protein. Phusion Flash DNA Polymerase
is free of contaminating endo- and exonucleases.
Dna amplification assay: Performance in PCR is tested by the
amplification of a 7.5 kb fragment of genomic DNA and a 20 kb
fragment of lambda DNA.
7. References
1. Nord K. et al. (1997) Nature Biotechnol. 15: 772–777.
2. Wikman M. et al. (2004) Protein Eng. Des. Sel. 17: 455–462.
3. Frey M. & Suppmann B. (1995) Biochemica 2: 34–35.
Shipping and storage
Phusion DNA Polymerase is shipped on gel ice. Upon arrival, store the
components at -20°C.
Technical support
US: techservice.genomics@thermofisher.com
Europe, Asia, Rest of World:
techservice.emea.genomics@thermofisher.com
Web: www.thermoscientific.com/phusion
Tm-calculator: www.thermoscientific.com/pcrwebtools
Product use limitation
This product has been developed and is sold exclusively for research purposes and
in vitro use only. This product has not been tested for use in diagnostics or drug
development, nor are they suitable for administration to humans or animals.
notice to Purchaser: Limited License
The purchase price of this product includes a limited, non-transferable license under
national patents from EP 0 547 920 B1, owned by New England Biolabs, Inc., to use this
product. No other license under these patents is conveyed expressly or by implication
to the purchaser by the purchase of this product.
The purchase price of this product includes a limited, nontransferable license under U.S.
and foreign patents owned by BIO-RAD Laboratories, Inc., to use this product. No other
license under these patents is conveyed expressly or by implication to the purchaser
by the purchase of this product.
Use of this product is covered by US Patent No. 6,127,155. The purchase of this product
includes a limited, non-transferable immunity from suit under the foregoing patent
claims for using only this amount of product for the purchaser’s own internal research.
No right under any other patent claim, no right to perform any patented method and no
right to perform commercial services of any kind, including without limitation reporting
the results of purchaser’s activities for a fee or other commercial consideration, is
conveyed expressly, by implication, or by estoppel. This product is for research use only.
Diagnostic uses under Roche patents require a separate license from Roche. Further
information on purchasing licenses may be obtained by contacting the Director of
Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California
This product is sold under license from Affibody AB, Sweden.
Designed and manufactured according to certified ISO9001:2008 processes.
Affibody ® is a registered trademark of Affibody AB, Sweden.
© 2011 Thermo Fisher Scientific Inc. All rights reserved. All other trademarks are the
property of Thermo Fisher Scientific Inc. and its subsidiaries.
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