High Throughput Solubility Measurements

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Precipitative <strong>Solubility</strong> - Outline of method: • Precipitative solubility from 5% 10mM DMSO stock solutions in pH7.4 PBS. • Experiment performed in 96 well format. • Quantification of DMSO stock solution and aqueous solution by flow-inject CLND calibrated against Caffeine. • Molecular Nitrogen count calculated using FindNN (written by John Hollerton)
DMSO stock plate Controls and Standards added via 8 tip LiHa 150ul transferred to optical plates via RoMa 96 tip ( TeMo) Transferred to Spectramax 384+ 5ul DMSO +195ul Unisol into MAR4 96 tip TeMo 5ul DMSO + 195ul Universal solvent into optical plate 96 tip TeMo <strong>Solubility</strong> 60 minute Incubation Transfer to 96 well Nunc via TeVac Check plate (CHK_A) Uni_A PBS_A 10ul DMSO +190ul PBS into MAR4 96 tip TeMo
Page 1 and 2: High-Throughput Solubility Alan Hil
Page 3 and 4: Solubility - From Solid ‘The gold
Page 5 and 6: Solubility from DMSO stock solution
Page 7 and 8: Aqueous Solubility Enhancement usin
Page 9 and 10: Precipitative Solubility…. • So
Page 11 and 12: How do we make a method High-throug
Page 13 and 14: How do we make a method High-throug
Page 15 and 16: DMSO stock plate 5ul DMSO +195ul PB
Page 17 and 18: Comparison of precipitative solubil
Page 19 and 20: Concentration mM 45 40 35 30 25 20
Page 21 and 22: Precipitative Solubility…. • Ho
Page 23 and 24: HP1100 with Antek 8060C CLND
Page 25 and 26: CLND - Chemi-luminescent Nitrogen d
Page 27: The key to the successful implement
Page 31 and 32: DMSO stock plate Controls and Stand
Page 33 and 34: Results:cont.. CLND response 2500.0
Page 35 and 36: Results:cont.. Aqueous solubility u
Page 37 and 38: Advantages of method: • Fast - 16
Page 39 and 40: Dual Injector twin channel CLND sol
Page 41: Acknowledgements: • Carol Carmody

High Throughput Solubility Measurements

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