Tomato CDI as a Strong Chymotrypsin Inhibitor ... - Plant Physiology

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Figure 6 A Remaining activity (%) B Remaining activity (%) 100 50 100 0 0.0 50 0 0.0 5.0 5.0 10.0 15.0 Inhibitor-to-protease molar ratio (fold) 10.0 15.0 Inhibitor-to-protease molar ratio (fold) 20.0 20.0 Trypsin + JIP-21 + STI + BBI Chymotrypsin + JIP-21 + STI + BBI
STI : JIP21 : WCI : STI : JIP21 : WCI : STI : JIP21 : WCI : STI : JIP21 : WCI : Figure 7 * 20 * 40 * 60 -MKSTIFFLFLFCAFTTS----------YLPSAIAD--FVLDNEGNPLENGGTYYILSDIT-AFG MMKCLFLLCLCLFPIVVFSSSFTSQNPIELPSASPKPNPVLDTNGNELNPNSSYRIISTFWGALG -MKSTTFLALFLLSAIIS----------HLPSSTADD-DLVDAEGNLVENGGTYYLLPHIW-AHG * 80 * 100 * 120 * G---IRAAPTGNERCPLTVVQSRNELDKGIGTIISSPYRIRFIAEGHPLSLKFDSFAVIMLCVGI GDVYLGKSPRSSAPCLDGVFRYNSDVG-TVGTPVRFIPLSGGIFEDQLMNLQFN-IATVKLCVSY GG--IETAKTGNEPCPLTVVRSPNEVSKGEPIRISSQFLSLFIPRGSLVALGFAN-PPS--CAAS 140 * 160 * 180 * PTEWSVVEDLPEGPAVKIGENKDAMDG--WFRLERVSDDEFNNYKLVFCPQQ--------AEDDK T--IWKAGNLNAYYRAMLLETGGSIGQVDSSYFKIVKASTFG-YNLLYCPITRPVLCPFCRGDDF P--WWTVVDSPQGPAVKLSQQKLPEKDILVFKFEKVSHSNIHVYKLLYCQHD--------EEDVK 200 * 220 * 240 CGD-IGISIDHDDGTRRLVVSKNKPLVVQFQKLDKESLAKKNHGLSRSE CAK-VGV-INQD-GRRRLALVNENPLGVYFKKV---------------- CDQYIGIHRDRN-GNRRLVVTEENPLELVLLKAKSETASSH-------- : 216 : 220 : 207 : 51 : 65 : 52 : 113 : 128 : 112 : 168 : 190 : 167
Page 1 and 2: Plant Physiology Preview. Published
Page 3 and 4: FOOTNOTES: This work has been suppo
Page 5 and 6: INTRODUCTION Plants respond to inse
Page 7 and 8: RESULTS Purification of the JIP21 p
Page 9 and 10: control of the periodic acid/Schiff
Page 11 and 12: Figure 8B. These levels are not due
Page 13 and 14: the cDNA described by Werner et al.
Page 15 and 16: characterization of our protein and
Page 17 and 18: membranes were rinsed in methanol f
Page 19 and 20: CCGAATTCATATGATGAAGTGTTTATTT-3’)
Page 21 and 22: Christeller JT, Farley PC, Ramsay R
Page 23 and 24: Hannapel DJ (1993) Nucleotide and d
Page 25 and 26: León J, Rojo E, Sánchez-Serrano J
Page 27 and 28: Samach A, Hareven D, Gutfinger T, K
Page 29 and 30: Yamamoto K (1995) Cathepsin E and C
Page 31 and 32: stained with Ponceau-S. C) Proteins
Page 33 and 34: Figure 1 A B 60 30 20 15 10 kDa Con
Page 35 and 36: Figure 3 Roots Stem Leaves Flowers
Page 37: Figure 5 A Cathepsin D Remaining ac

Tomato CDI as a Strong Chymotrypsin Inhibitor ... - Plant Physiology

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