III Congreso Ib%C3%A9rico del Lobo

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Resúmenes III Congreso Ibérico del Lobo Hematological and serum biochemical reference values of freeranging Iberian wolves in Portugal C. Ferreira, H. Rio-Maior, S. Roque, F. Petrucci-Fonseca, R. Brandão, N. Santos & F. Álvares Health assessment of wildlife is particularly important for threatened and protected species, such is the case of the Iberian wolf in Portugal. An assessment of wolf population health status can be obtained from the evaluation of hematological and serum biochemical parameters and its comparison with other wolf populations under similar ecological conditions. These reference intervals are published for some North American and European populations of gray wolf, but to our knowledge, no information concerning Iberian wolf blood parameters is available. The objective of this study is to provide the first available data on health condition of free-ranging Iberian wolves through the establishment of hematology and clinical biochemistry reference intervals. Blood samples were obtained from 13 individuals captured and chemically immobilized for collaring as part of wolf ecology studies, from 2007 through 2012. Samples were stored at 4 ºC for 1–3 days before laboratory analysis. Reference intervals were calculated as mean and standard deviation for 14 hematologic and 20 biochemical analyses. When compared to reference values available from other wolf populations, our results show: i) low hemoglobin (15,1 ± 3,0 g/dL) possibly related to food availability or social status; ii) high creatine kinase activity (3.238,4 ± 1.379,5 IU/L) due to heavy exercise and moderate muscle injury associated with capture; iii) stress leukogram, with leukocytosis (17,84 ± 4,78 x 10 9 /L), neutrophilia (15,41 ± 4,85 x 10 9 cells/L), lymphopenia (1,25 ± 0,56 x 10 9 cells/L), monocytosis (0,85 ± 0,40 x 10 9 cells/L) and eosinopenia (0,27 ± 0,17 x 10 9 cells/L) as well as high alanine aminotransferase (ALT) activity (88,4 ± 40,4 IU/L), associated with acute or chronic stress; and iv) high urea concentration (58,0 ± 22,0 mg/dL) which may be related to probable influence of diet. The small number of samples in this study, although a limiting factor, is representative of two important Iberian wolf subpopulations (one in northwestern Portugal and a small isolated subpopulation south of the Douro River). Other limitations are the impossibility to know the nutritional status (time since last meal) of each animal, the interference of the capture stress in some parameters and the seasonal variation of some parameters. On the other hand, an in-field medical examination of each animal allows us to correlate the analytical results with general health condition. 25 ORAL - 13
ORAL - 14 III Congreso Ibérico del Lobo Resúmenes Metodologías destructivas y no destructivas en Ecotoxicología: niveles de metales pesados en diversos órganos de lobo ibérico (Canis lupus signatus) I. Ponce Hernández, I. Rodríguez, F. Matías, D. Hernández-Moreno, F. Soler Rodríguez, L. Llaneza, A.M. López-Beceiro, L.E. Fidalgo & M. Pérez-López La Ecotoxicología estudia el efecto de los agentes químicos sobre los seres vivos, especialmente a nivel de las poblaciones, comunidades y/o ecosistemas. El lobo ibérico es un consumidor situado en la cumbre de la cadena trófica, lo que le lleva a acumular diferentes contaminantes ambientales en sus tejidos. El uso de esta especie como bioindicadora para determinar los efectos adversos de los metales puede ser por tanto de enorme interés en las actividades de biomonitorización. En el presente estudio se analizan los niveles de metales pesados (Pb, Cd y Zn) en distintas muestras destructivas (hígado y riñón) y no destructivas (pelo) de lobo, considerando tanto el sexo como la edad de los animales. Las muestras fueron sometidas a digestión por vía húmeda, determinándose el contenido de metales por Espectrometría de Masas con Plasma Acoplado Inductivamente (ICP-MS). Con respecto al total de la población, los niveles más elevados correspondieron al metal esencial Zn, con un máximo en las muestras de pelo, y un mínimo en riñón (
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III Congreso Ib%C3%A9rico del Lobo

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