III Congreso Ib%C3%A9rico del Lobo

ORAL - 22
III Congreso Ibérico del Lobo Resúmenes
Paternal lineage diversity of modern wolves and dogs in Iberia
F.P.S. Simões de Matos, A.E. Pires, C.C. Santos Borges, I. Amorim do Rosário,
T. Teixeira, F. Petrucci-Fonseca & J. Matos
Presently, in Iberia both Canis lupus signatus, a gray wolf subspecies native to
Iberia, and the Canis lupus familiaris, the domestic dog, coexist. The domestic
dog has existed in this territory at least since 8,000 YBP, and modern breed dogs
natives to the Iberian Peninsula show a wide variety of forms and functions. The
Iberian wolf has suffered a dramatic regression of its population size and geographic
distribution in the last decades, being this subspecies nowadays mainly restricted
to the northwest region of the Iberia. Both species have been the focus of
several genetic studies using powerful molecular markers useful to describe their
genetic composition, genetic variability, population structure and phylogeny
such as STRs, AFLPs, mtDNA and Y-STRs, but not Y-SNPs. Paternal genetic
markers were used here to investigate the genetic diversity and structure of Iberian
wolves and native domestic dogs from Iberia and North Africa. Dog specific
Y-chromosome SNPs (n=11) and Y-chromosome STRs (n=4) were used. A total
of 108 canids, 81 male dogs including breed dogs from Iberia, North Africa and
village dogs, and 27 wild and captive Iberian male wolves were analysed. Based
on Y-SNPs we detected a single founder patrilineage in the studied dogs and four
patrilineages were detected in the Iberian wolves. In the worldwide context, these
four patrilineages were only detected in the Iberian wolf subspecies. Y-STRs revealed
additional haplotypes in the studied dogs (n=25) and in the Iberian wolf
(n=12). Contemporary samples provide evidence of a significant genetic structure
between these two species: Y-SNPs haplotypes differ by three mutations,
one transition and two transversions, and using Y-STRs we obtained a PhiPT
value of 0.27 (P=0.001). Although threatened, the Iberian wolf still represents
a genetic diversity reservoir for the global species Canis lupus. These results are
supportive of better and more intensive plans for its preservation in the wild.
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