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iTRAQ - Inserm

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Marquage <strong>iTRAQ</strong><br />

Échantillon 1 Échantillon 2 Échantillon 3 Échantillon 4<br />

Digestat 1 Digestat 2<br />

Réduction, alkylation, digestion<br />

iTraq 114 iTraq 115 iTraq 116 iTraq 117<br />

Digestat 114 Digestat 115 Digestat 116<br />

Digestat 117<br />

Mélange des peptides<br />

Séparation 2D:<br />

1ère dimension: SCX ou OFFGEL<br />

2ème dimension : nanoLC sur C 18<br />

Digestat 2 Digestat 2<br />

Analyse MS/MS sur MALDI TOF/TOF

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