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O - laboratoire PROTEE - Université du Sud - Toulon - Var

O - laboratoire PROTEE - Université du Sud - Toulon - Var

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Protocole d’extraction des EPS (2 ème partie)<br />

CT4<br />

Extraction CER (Dowex)<br />

Centrifugation – 12 000 g – 4°C – 1 min<br />

Résine + qq bactéries SN4<br />

CT5<br />

Bactéries + reste résine<br />

CT6<br />

Bactéries<br />

Bactéries <strong>du</strong><br />

biofilm dans leur<br />

matrice<br />

exopolymérique<br />

Centrifugation – 15 000 g – 4°C – 1 h<br />

Centrifugation – 15 000 g – 4°C – 1 h<br />

Filtration (0, 22µm)<br />

Dialyse (3500 Da)- 3j – 4°C<br />

SN6<br />

EPS-liés<br />

SN5<br />

Cadre <strong>du</strong> stage<br />

2 souches (TC9 et FRN1)

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