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Présentation des outils du laboratoire: l t h i h t hi les techniques ...

Présentation des outils du laboratoire: l t h i h t hi les techniques ...

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CHROMATOGRAPHIE LIQUIDE HAUTE<br />

PERFORMANCE (HPLC)<br />

◦ Dosage d’un composé: exemple de la Simalikalactone D<br />

1. Courbe de calibration<br />

Concentration (mg/mL) Aire <strong>du</strong> pic<br />

0 0<br />

0,0061 137258<br />

1,22 13933630<br />

84000000<br />

305 3,05 38926414<br />

6,1 69117131 70000000<br />

56000000<br />

42000000<br />

28000000<br />

14000000<br />

0<br />

Aire <strong>du</strong> pic<br />

Courbe de calibration<br />

0 1 2 3 4 5 6 7<br />

Concentration (mg/ml)<br />

Droite de régression: Y= 11611021,7856 X<br />

Quassine<br />

SkD<br />

Quassine<br />

SkD<br />

Bertani,S.,Houël,E.,Bourdy,G.,Stien,D.,Landau,I.,Deharo,E.,Quassia amara L. (Simaroubaceae) leaf tea: effect of<br />

the growing stage and <strong>des</strong>sication status on the antimalarial activity of a traditional preparation, J.<br />

Ethnopharmacol.,(2007), 111, 40-42

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