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Setiap enzim restriksi (endonuklease restriksi) mengenalsekuen pemotongan yang khas dan memotong <strong>DNA</strong> padasitus pemotongan yang khas.Nama Enzim Sekuens Pengenal Organisme asalEcoRI G↓AATTC Escherichia coliHindIII A↓AGCTT Haemophilus influenzaeHhaI GCG↓C Haemophilus haemolyticusTaqI T↓CGA Thermus aquaticusBsuRI GG↓CC Bacillus subtilisBalI TGG↓CCA Brevibacterium albidumNotI GC↓GGCCGC Nocardia otidiscaviarumBamHI G↓GATCC Bacillus amyloliquefaciensSmaI CCC↓GGG Serratia marcescens9