IN Cell Analyzer 2200
IN Cell Analyzer 2200
IN Cell Analyzer 2200
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
<strong>IN</strong> <strong>Cell</strong> <strong>Analyzer</strong> <strong>2200</strong><br />
<strong>IN</strong> <strong>Cell</strong> <strong>Analyzer</strong> <br />
<br />
<br />
<br />
<strong>IN</strong> <strong>Cell</strong> <strong>Analyzer</strong> <strong>2200</strong> sCMOS <br />
<br />
2013 1 <br />
<br />
<br />
<br />
NEW<br />
<br />
<br />
3,240 <br />
<br />
<strong>IN</strong> <strong>Cell</strong> <strong>Analyzer</strong><br />
<br />
<br />
FCM<br />
<br />
10 3<br />
Flow<br />
<strong>Cell</strong><br />
10<br />
<br />
2<br />
OrangePM1<br />
10 4 10 1 102<br />
10<br />
1<br />
<br />
10 0<br />
<br />
<br />
<br />
<br />
<br />
<br />
10 0<br />
GreenPM3<br />
10 3 104<br />
<br />
<br />
<br />
<br />
OrangePM1<br />
10 3<br />
10 2<br />
10 1<br />
Flow<br />
<strong>Cell</strong><br />
/ <br />
10 0<br />
10 0<br />
10 4 10 1 102<br />
GreenPM3<br />
10 3 104<br />
! !
HeLa 10 µM01 µM Nocodazole 10 µM01 µM Paclitaxel 24 PI <br />
<strong>IN</strong> <strong>Cell</strong> <strong>Analyzer</strong> PI <br />
<br />
Nocodazole 01 µM Medium <br />
10 µM G2M <br />
Medium<br />
PacliTaxel 0.1 µM<br />
G2/M 26.7%<br />
G1 61.1%<br />
G2/M 28.1%<br />
G1 59.6%<br />
Medium G2/M 26.7% S 7.2%<br />
G1 61.1%<br />
G2/M 28.1%<br />
G1 59.6%<br />
G2/M G2/M 26.7% 26.7%<br />
Medium<br />
G1 G1 61.1% 61.1% DMSO<br />
S 7.8%<br />
G2/M 26.7%<br />
G1 61.1%<br />
G2/M 28.1% G2/M 26.7% G1 59.6% G1 61.1%<br />
S 7.2%<br />
S 7.8%<br />
S 7.2% 7.2%<br />
S 7.2%<br />
S 7.8% S 7.2%<br />
G1 12.9%<br />
G2/M 24.1%<br />
G1 62.0%<br />
S 16.9%<br />
Nocodazole 0.1 µM G1 12.9% Paclitaxel 0.1 µM<br />
G2/M 24.1%<br />
G1 12.9%<br />
S 6.5%<br />
G1 62.0%<br />
G2/M 59.7% G2/M G2/M 24.1% 24.1% S 16.9% G1 G1 62.0% 62.0%<br />
G2/M 24.1%<br />
G1 62.0%<br />
G2/M 24.1%<br />
S 16.9%<br />
G1 62.0%<br />
S 6.5%<br />
G2/M 59.7%<br />
S 6.5% 6.5%<br />
S 6.5%<br />
G2/M 59.7% S 6.5%<br />
G1 10.8%<br />
Nocodazole 1.0 µM Paclitaxel 1.0 µM<br />
G1 11.7%<br />
G1 S 10.8% 6.7%<br />
G1 10.8%<br />
G2/M 71.9%<br />
G1 S 11.7% 4.5%<br />
S 6.7%<br />
G1 11.7%<br />
S 6.7% G1 G1 11.7% 11.7%<br />
G2/M 71.9%<br />
G1 11.7%<br />
G2/M 69.2%<br />
S 4.5%<br />
G2/M 71.9%<br />
S 4.5% 4.5%<br />
S 4.5%<br />
S 4.5%<br />
G2/M 69.2%<br />
PI <br />
G2/M G2/M 69.2% 69.2%<br />
G2/M 69.2%<br />
G2/M 69.2% PI <br />
G2/M G2/M 28.1% 28.1%<br />
G2/M 28.1%<br />
S 7.8% 7.8%<br />
S 7.8%<br />
G2/M G2/M 59.7% 59.7%<br />
G2/M 59.7%<br />
G2/M G2/M 71.9% 71.9%<br />
G2/M 71.9%<br />
G1 G1 59.6% 59.6%<br />
G1 59.6%<br />
G1 G1 12.9% 12.9%<br />
G1 12.9%<br />
S 16.9% 16.9%<br />
S 16.9%<br />
G1 G1 10.8% 10.8%<br />
G1 10.8%<br />
S 6.7% 6.7%<br />
S 6.7%<br />
<br />
<br />
<br />
<br />
<br />
DDC CK19 <br />
<br />
3 ControlGene1Gene2<br />
Gene1Gene2 CK19 <br />
CK19 <br />
<br />
<br />
Control<br />
6%<br />
Control<br />
<br />
94%<br />
2<br />
Gene-1<br />
Gene-1<br />
11%<br />
Count<br />
89%<br />
Gene-2<br />
Gene-2<br />
11%<br />
89%<br />
20 <br />
Binned <strong>Cell</strong>s, <strong>Cell</strong> Intensity<br />
CK19 <br />
CK19
LIFChemical X <br />
MAP2GFAP<br />
<br />
<br />
<br />
<br />
Control<br />
LIF<br />
008 pgml<br />
LIF<br />
80 ngml<br />
<br />
Hoechst 33258<br />
<br />
anti-MAP2<br />
<br />
anti-GFAP<br />
Fused<br />
<br />
96 LIF <strong>IN</strong> <strong>Cell</strong> <strong>Analyzer</strong> 96 <br />
<br />
<br />
<br />
<br />
MAP2 <br />
GFAP <br />
40 <br />
96 <br />
<br />
<br />
1 <br />
1 96 96 <br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<strong>IN</strong> <strong>Cell</strong> <strong>Analyzer</strong> Image Competition <br />
<br />
Web <br />
www.gelifesciences.co.jp
1<br />
/ <br />
<br />
<br />
6 1536 <br />
<br />
<br />
<br />
<br />
<strong>IN</strong> <strong>Cell</strong> <strong>Analyzer</strong><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
2<br />
<br />
<br />
3<br />
<br />
<br />
<br />
<br />
<br />
Wholewell<br />
<br />
<br />
<br />
<br />
<strong>IN</strong> <strong>Cell</strong> <strong>Analyzer</strong> <br />
1/2<br />
<br />
Stephen Minger, Ph.D<br />
ES Stem <strong>Cell</strong> Biology <br />
2009 9 GE Healthcare <br />
<br />
www.gelifesciences.co.jp<br />
e-mail Web <br />
8<br />
2013 1 <br />
<br />
<br />
<br />
<br />
71-3358-03
![[PDF] マニュアル GradiFrac](https://img.yumpu.com/22037825/1/190x253/pdf-gradifrac.jpg?quality=85)
![[PDF] Sample preparation for analysis of protein, peptides and ...](https://img.yumpu.com/21549715/1/190x257/pdf-sample-preparation-for-analysis-of-protein-peptides-and-.jpg?quality=85)
![[PDF] Data File: rProtein A Sepharose Fast Flow](https://img.yumpu.com/21549316/1/190x253/pdf-data-file-rprotein-a-sepharose-fast-flow.jpg?quality=85)
![[PDF] MBP-tagged protein purification](https://img.yumpu.com/21548507/1/184x260/pdf-mbp-tagged-protein-purification.jpg?quality=85)
![[PDF] AKTA ready system Data file](https://img.yumpu.com/21540925/1/190x253/pdf-akta-ready-system-data-file.jpg?quality=85)
![[PDF] Data File - rProtein A/Protein G GraviTrap](https://img.yumpu.com/21539052/1/190x253/pdf-data-file-rprotein-a-protein-g-gravitrap.jpg?quality=85)
Change language