Autosampler A-900 and A-905 - GE Healthcare Life Sciences

GE Healthcare
Data file 18-1177-97 AB
Liquid chromatography system accessories
Autosampler A-900 and A-905
Autosampler A-900 and A-905 allow automatic and accurate
sample injection by using a novel method of reproducible
sample withdrawal of sample volumes from 0.1 µl up to 1 ml.
Autosampler A-900 is available in two options—with or without
Peltier cooling. The cooling option effectively protects heatsensitive
biomolecules from degradation during sampling.
Autosampler A-905 is also available in two options—
Autosampler A-905 for ÄKTA and Autosampler A-905
for ÄKTAmicro liquid chromatography systems. Both
Autosampler A-905 options are fitted with cooling plates
as standard.
Autosampler A-900 and A-905 offer a choice of three
different injection methods giving different balances
between reproducibility and accuracy. The autosamplers
are designed to allow use of a variety of vials and septa
and sample loss is insignificant due to the novel sample
withdrawal method.
Autosampler A-900 and A-905 are controlled simply and
directly by UNICORN as an integral part of ÄKTAexplorer,
ÄKTApurifier, ÄKTAfplc, or ÄKTAmicro systems.
Autosampler A-900 and A-905 are:
Reliable and easy to use
• Excellent reproducibility from each of three different
sample injection methods
• Simple programming using UNICORN control software
Flexible
• Multiple choice of sample injection methods and volumes,
as well as a variety of vials and septa
Designed to minimize contamination
• Separate needles for sampling and puncturing of septa
• Buffer tubing between syringe and injection valves
eliminates contamination
Fig 1. Autosampler A-900 is available with or without Peltier cooling option.
Fig 2. Autosampler A-905 is fitted with a Peltier cooling element and is
available in two options: A-905 for use with ÄKTA design; or A-905 for use
with ÄKTAmicro chromatography systems.
imagination at work

Excellent reproducibility using different
sample injection methods
Each of the three selectable sample injection methods for
Autosampler A-900 and A-905 provide excellent sampling
reproducibility:
• Flushed loop: Sample loop is completely filled with
sample. Sampling reproducibility using this method is
better than 0.3% RSD (relative standard deviation).
• Partial loopfill: Sample loop is partially filled with sample
allowing programmable injection volumes. Partial loopfill
sample injection provides sampling reproducibility better
than 0.5% RSD.
• µl pick-up: Sample volume is transported into the loop with
transport liquid (mobile phase) after aspiration from the vial.
Using this method offers minimal sample loss and maximum
accuracy with a reproducibility better than 1% RSD.
Different dispenser syringe sizes and
injection volumes provide greater flexibility
Four sizes of dispenser syringes are available for
Autosampler A-900 and A-905: 100, 250, 500, and 1000 µl.
Injection volumes for Autosampler A-900 range from a
few microliters to 1 ml. Autosampler A-905 for ÄKTA allows
injection volumes from 0.1 µl to 1 ml, making it an excellent
choice for the small sample volumes injected during
analytical purification.
Simple programming from UNICORN
Autosampler A-900 is remotely controlled by UNICORN
software. The UNICORN method wizard allows fast and
easy programming by the user and includes extensive data
handling features (Fig 3). For Autosampler A-900 and
A-905, an extended evaluation package, UNICORN analysis
module, provides:
• Quantitation by internal or external standards
• Determination of molecular size by gel filtration
• Quantitation of sample amount and sample
concentration
• Recovery calculations
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Multiple choice of sample vials
Almost every type of vial with an outer diameter (o.d.) of
12 mm or 7 mm can be used with Autosampler A-900. By
using inserts, volumes of standard vials can be reduced.
Two types of tray segments are available for Autosampler
A-900; standard trays, and microvial trays (Fig 4).
Autosampler A-900 is fitted with eight of the standard
12 mm tray segments. Each segment holds 12 vials giving a
maximum capacity of 96 vials. Each microvial tray segment
holds twenty 7-mm vials giving a maximum capacity of
160 vials.
Autosampler A-905 is supplied with a 96-well low microplate
and 96-well high microplate for 7 mm vials, a 384-well
low microplate, 48-vial adapter for 12 mm standard vials,
and 10 ml transport solvent/wash vials (Fig 5). Samples
can be directly applied to the microplates or to microvials
positioned on the plates.
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Fig 4. The two types of tray segments available for the A-900 are (top)
standard vials of o.d. 12 mm and (bottom) microvials of o.d. 7 mm.
Fig 3. Simple programming using the method wizard of UNICORN control
software.
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Fig 5. Microplates for Autosampler A-905: (A) 384-well; (B) 48-vial adapter;
(C) 96-well.
Autosampler A-900 and A-905 minimize
contamination
Separate needles for puncturing and sampling
An air needle and sample needle are incorporated in
Autosampler A-900 and A-905; the air needle punctures
the septum of the vial and provides headspace pressure,
while the sample needle transports sample from the vial
into the loop of the valve. PEEK sample needles are available
as an alternative to the steel sample needle for use with
biosensitive materials.
Buffer tubing between syringe and injection valves
minimizes contamination
Autosampler A-900 and A-905 use a syringe to aspirate
the sample from a vial into the sample loop. To prevent
contamination of the syringe, the autosamplers are
equipped with buffer tubing between the syringe and the
injection valve. The wash solvent removes the sample from
the buffer tubing and the sample needle and also rinses
both components.
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Technical specifications
Autosampler A-900*
Sampling
Sample capacity
Standard tray:
96 vials of 1.5 ml,
12 vials per segment
Microvial tray:
160 vials of 0.5 ml,
20 vials per segment
Vial dimensions (cap included) Maximum vial height: 47 mm
Minimum vial height: 32 mm
Loop volume 5–1000 µl
Dispenser syringe
100 µl, 250 µl (standard), 500 µl, or
1000 µl syringe
Vial detection
Missing vial detection by vial sensor
Headspace pressure
Built-in compressor
Injection valve switching time < 100 ms
Piercing precision needle ± 0.6 mm
Wash solvent
250 ml internal wash solvent bottle
Wetted parts
PEEK, stainless steel, PTFE, glass,
Tefzel, alumina, ceramic, and
inert polymer
Analytical performance
Reproducibility
Memory effect
Environment
< 0.3% RSD for flushed loop
injections
< 0.5% RSD for partial loopfill
injections > 5 µl injection volumes
(5 µl sample, 100 µl sample loop,
< 2.5 MPa; 10 µl sample, 20 µl
sample loop, < 25 MPa)
< 1.0% RSD for µl pick-up injections
> 5 µl injection volumes
< 0.1% with programmable
needle wash
Operational conditions +4°C to +40°C, 20%–95% relative
humidity
Sound pressure level < 70 dB (A)
Installation category Category II, according to IEC 1010
Pollution degree Degree 2, according to IEC 1010
Dimensions
Dimensions (W × H × D) 280 × 440 × 540 mm
Weight
A–900 22 kg
A–900 with cooling 30 kg
Power requirements
Power supply voltage 100–240 V, 50–60 Hz
Power requirement
250 VA
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Communication
Serial communication port UniNet-1
Additional specifications for Autosampler A-900 with cooling †
Cooling capacity
> 11.5°C below ambient
temperature (T) for
16°C < T < 40°C
Typical cooling times 34 min from 23°C to 4°C
(at 45% relative humidity)
60 min from 32°C to 4°C
(at 35% relative humidity)
Autosampler A-905 ‡
Sampling
Sample capacity
96-well microplate (low/high):
Direct filling or 96 microvials
384-well microplate:
Direct filling or 384 microvials
48-vial adapter:
48 vials
Vial dimensions (cap included) Maximum vial height: 47 mm
Minimum vial height: 12.5 mm
Loop volume 5–1000 µl
Dispenser syringe
100 µl (standard in A-905 for
ÄKTAmicro, 250 µl, 500 µl, 1000 µl
(standard in A-905 for ÄKTA)
Plate detection
Missing plate detection by vial
sensor
Headspace pressure
Built-in compressor
Injection valve switch time < 100 ms
Piercing precision needle ±0.6 mm
Wash solvent
100 ml internal wash solvent bottle
Wetted parts
PEEK, PTFE, glass, Tefzel, alumina,
ceramic, and inert polymer
Dimensions
Dimensions (W ×H × D) 280 × 440 × 400 mm
Weight
26 kg
Power requirements
Power supply voltage 100–240 V, 50–60 Hz
Power requirement
250 VA
Communication
Serial communication port UniNet-1
* Autosampler A-900 without cooling requires UNICORN v2.3 or higher.
†
Autosampler A-900 with cooling requires UNICORN v4.0 or higher.
‡
Autosampler A-905 for ÄKTA requires UNICORN v4.12 or higher.
Analytical performance
Reproducibility
Memory effect
Cooling capacity
< 0.3% RSD for flushed loop
injections> 5 µl injection volumes
< 0.5% RSD for partial loop fill
injections > 5 µl injection volumes
(5 µl sample, 100 µl sample loop,
< 2.5 MPa)
< 1.0% RSD for µl pick-up
injections > 5 µl injection volumes
< 0.1% with programmable
needle wash
Max. 12°C below ambient
temperature (T) for 16°C < T < 40°C
Environment
Operating conditions
+4°C to +40°C, 20%–95% relative
humidity
Sound pressure level < 70 dB (A)
Installation category Category II, according to IEC 1010
Pollution degree Degree 2, according to IEC 1010
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Ordering information
Autosampler units
Product Quantity Code No.
Autosampler A-900 with cooling 1 18-1144-61
Autosampler A-900 without cooling 1 18-1116-61
Autosampler A-905 for ÄKTA 1 18-1175-93
Autosampler A-905 for ÄKTAmicro 1 18-5050-65
Accessories and consumables
Tray segment set 1.5 ml vial (o.d. 12 mm) 1 18-1119-90
Tray segment set 0.5 ml vial (o.d. 7 mm) 1 18-1120-48
Rheflex ferrule 10 18-1119-91
Rheflex nut 10 18-1119-92
Flange tube fitting 1/8” 5 18-1119-93
Flange tube fitting 1/16” 5 18-1119-94
Plunger replacement tip 100 µl 1 18-1119-96
Plunger replacement tip 500 µl 1 18-1119-97
Plunger replacement tip 250 µl 1 18-1119-98
PEEK sample needle, 15 µl 1 18-1119-99
Sample needle, fused silica, 5 µl 1 18-1158-68
Stainless steel sample needle 1 18-1148-65
Buffer tubing 2000 µl 1 18-1120-31
Buffer tubing 500 µl 1 18-1120-32
Wash solvent bottle 250 ml 1 18-1120-33
Syringe 100 µl 1 18-1120-34
Syringe 250 µl 1 18-1120-35
Syringe 500 µl 1 18-1120-36
Syringe 1000 µl 1 18-1120-37
Luer lock connection, female 1 18-1120-38
PEEK loop 20 µl 1 18-1160-49
PEEK loop 100 µl 1 18-1120-40
Syringe waste tubing 1 18-1120-44
Syringe tubing 1 18-1120-45
Wash vial 1 18-1120-46
Union nut air 1 18-1120-47
Stop plug, 1/16” 5 18-1112-52
Union Luer female/1/16” male 2 18-1112-51
Union 1/16” female/M6 male 6 18-1112-57
Union M6 female/1/16” male 8 18-1112-58
Software
UNICORN Analysis Module, v5.0 1 11-0003-60
UNICORN control software, v5.20:
Remote license* 1 28-9432-57
Workstation license 1 28-9432-44
* Requires existing workstation.
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For local office contact information, visit
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www.gelifesciences.com/akta
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751 84 Uppsala
Sweden
GE, imagination at work, and GE monogram are trademarks of General Electric Company.
ÄKTA, ÄKTAexplorer, ÄKTAfplc, ÄKTAmicro, ÄKTApurifier, and UNICORN are trademarks of
GE Healthcare companies.
All third party trademarks are the property of their respective owners.
© 2003-2009 General Electric Company—All rights reserved.
First published Oct. 2003
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imagination at work
18-1177-97 AB 06/2009