Autosampler A-900 and A-905 - GE Healthcare Life Sciences

Excellent reproducibility using different
sample injection methods
Each of the three selectable sample injection methods for
Autosampler A-900 and A-905 provide excellent sampling
reproducibility:
• Flushed loop: Sample loop is completely filled with
sample. Sampling reproducibility using this method is
better than 0.3% RSD (relative standard deviation).
• Partial loopfill: Sample loop is partially filled with sample
allowing programmable injection volumes. Partial loopfill
sample injection provides sampling reproducibility better
than 0.5% RSD.
• µl pick-up: Sample volume is transported into the loop with
transport liquid (mobile phase) after aspiration from the vial.
Using this method offers minimal sample loss and maximum
accuracy with a reproducibility better than 1% RSD.
Different dispenser syringe sizes and
injection volumes provide greater flexibility
Four sizes of dispenser syringes are available for
Autosampler A-900 and A-905: 100, 250, 500, and 1000 µl.
Injection volumes for Autosampler A-900 range from a
few microliters to 1 ml. Autosampler A-905 for ÄKTA allows
injection volumes from 0.1 µl to 1 ml, making it an excellent
choice for the small sample volumes injected during
analytical purification.
Simple programming from UNICORN
Autosampler A-900 is remotely controlled by UNICORN
software. The UNICORN method wizard allows fast and
easy programming by the user and includes extensive data
handling features (Fig 3). For Autosampler A-900 and
A-905, an extended evaluation package, UNICORN analysis
module, provides:
• Quantitation by internal or external standards
• Determination of molecular size by gel filtration
• Quantitation of sample amount and sample
concentration
• Recovery calculations
12
11
10
9
8
7
6
5
4
3
2
1
A1
Multiple choice of sample vials
Almost every type of vial with an outer diameter (o.d.) of
12 mm or 7 mm can be used with Autosampler A-900. By
using inserts, volumes of standard vials can be reduced.
Two types of tray segments are available for Autosampler
A-900; standard trays, and microvial trays (Fig 4).
Autosampler A-900 is fitted with eight of the standard
12 mm tray segments. Each segment holds 12 vials giving a
maximum capacity of 96 vials. Each microvial tray segment
holds twenty 7-mm vials giving a maximum capacity of
160 vials.
Autosampler A-905 is supplied with a 96-well low microplate
and 96-well high microplate for 7 mm vials, a 384-well
low microplate, 48-vial adapter for 12 mm standard vials,
and 10 ml transport solvent/wash vials (Fig 5). Samples
can be directly applied to the microplates or to microvials
positioned on the plates.
12
11
10
20
19
18
17
9
8
7
16
15
14
13
6
12
11
10
9
5
4
3
8
7
6
5
2
1
A1
4
3
21
D1
20
19
18
17
16
15
14
13
1
11
Fig 4. The two types of tray segments available for the A-900 are (top)
standard vials of o.d. 12 mm and (bottom) microvials of o.d. 7 mm.
Fig 3. Simple programming using the method wizard of UNICORN control
software.
2 28-9496-70 AA