Agilent ZORBAX 液相柱选择指南 - ä¸­å›½ç§‘å­¦é™¢ä¸Šæµ·ç»†èƒžç”Ÿç‰©å­¦ç ”ç©¶æ‰€

Agilent ZORBAX

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Agilent ZORBAX
HPLC............
pHZORBAX
3
1. 6 -9
2. www.agilent.com/chem/lccolumntips
3.
800-820-3278

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ZORBAX .................................................................................5
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ZORBAX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
ZORBAX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
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USP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
ZORBAX .........................................................................................19
ZORBAX Eclipse Plus. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
ZORBAX Eclipse XDB. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
ZORBAX 80Å StableBond . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
ZORBAX Rx . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
ZORBAX 80Å Extend-C18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
ZORBAX Bonus-RP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
ZORBAX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
ZORBAX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
ZORBAX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
ZORBAX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
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ZORBAX 1.8 µm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
ZORBAX 3.5 µm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
ZORBAX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
ZORBAX 1.0 mm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
ZORBAX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
ZORBAX PrepHT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Ultron . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
ZORBAX .....................................................................89
ZORBAX 300Å StableBond . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
ZORBAX 300Å Extend-C18 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
ZORBAX Poroshell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
ZORBAX Eclipse (AAA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
ZORBAX GF-250 GF-450 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
ZORBAX SAX SCX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

ZORBAX Eclipse Plus LC
Eclipse Plus
600 bar ZORBAX (RRHT) 1.8 µm LC
ZORBAX Eclipse LC
40
2
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Agilent 1200
20
1200 HPLC
40
Agilent 1200 ZORBAX RRHT 1.8 µm
www.agilent.com/chem/1200RR
LC 60%
600
30
60,000
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1200
ZORBAX RRHT
1200 HPLC
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3

ZORBAX Eclipse
ZORBAX Eclipse
Eclipse
Eclipse
Eclipse
1.8 µm 7 µm......
pH
1.8 µm 7 µm
600 bar ZORBAX 20
60%
4
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ZORBAX
USP
Agilemt ZORBAX
HT Eclipse Plus USP
pH pH
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5

2007-2008 LC
52-53
52-53
H 2 O
H 2 O
19
>2000
19
19
19
19
>2000
107
111
94
"Practical HPLC Methodology and Applications," Brian A. Bidlingmeyer, John Wiley & Sons, Inc., New York, p. 109
6
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ZORBAX
ZORBAX
ZORBAX RP-HPLC
Eclipse Plus
pH 2-9
1.83.5 5 µm
QA/QC
20-21
Eclipse XDB
StableBond (SB)
pH (pH 2-9)
1.8 3.5 µm
pH
C18 90°C C8C3 Aq
80°C pH
C18C8C3CNAq
(TFA) LC/MS
(TFA)
1.8 3.5 µm
26
33
ZORBAX Rx
Bonus-RP
Extend-C18
pH SB
Rx-C8 SB-C8
pH pH pH
3.5 µm
pKa pH
pH 11.5
LC/MS
pH
3.5 µm
39
45
41
ZORBAX
ZORBAX
pH SB
SB
pH
51
ZORBAX ODS Classic
pH SB
XDB
51
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7

ZORBAX
HPLC HPLC
ZORBAX
MW < 2000 MW > 2000
80-20Å
300Å
80-20Å
300Å (300SB)
Eclipse Plus C18
StableBond 300SB-C18
C18 C18
4.6 mm
4.6 mm
LC/MS
(mm)
4.6 0.1-1.5 mg 0.5-3 mL/min
3.0 150-500ug 0.3-1.5 mL/min + HPLC
2.1 50-120 ug 0.1-0.5 mL/min ++ LC/MS
1.0 10-50 ug 10-100 µL/min ++++ LC/MS
0.5, 0.3 1-10 ug 1-15 µL/min +++++ LC/MS
0.1, 0.075 100-200 ng 200-500 nL/min ++++++ LC/MS
9.4 1 -10 mg 5-10 mL/min mg
21.2 20-250 mg 20-60 mL/min 1
8

ZORBAX
MW < 2000 MW > 2000
5µm 5µm
5 µm
3.5µm
HT 1.8µm
3.5µm 5 µm 60%
1.8 µm 200%
3.5µm
Poroshell 5µm
3.5 µm
Poroshell
150 mm 150 mm
5 µm
150 mm 250 mm
20-30
(3.5 µm)
75 mm 50 mm
150mm
30-60
(3.5 µm) (50 mm)
Eclipse Plus C18
4.6 x 150 mm, 5 µm
959993-902
Eclipse Plus C18
4.6 x 75 mm, 3.5 µm
959933-902
ZORBAX 300SB-C18
4.6 x 150 mm, 5 µm
883995-902
ZORBAX 300SB-C18
4.6 x 50 mm, 3.5 µm
865973-902
600 bar
Eclipse Plus C18
4.6 x 50 mm, 1.8 µm
959941-902
ZORBAX Poroshell
300SB-C18
2.1 x 75 mm, 5 µm
660750-902
9

HPLC
5000 (60-100Å) 300Å
HPLC 5 µm 3.5 µm
1.8 µm 3.5 µm
1.8 µm 3.5 µm
LC 50 mm 1.8 µm RRHT
LC LC
400 bar
4.6 x 150 mm 4.6 x 75 mm
(4.6 x 250 mm)
2.13.0 mm
ZORBAX ZORBAX SIL ZOR-
BAX Rx-SIL ZORBAX SIL ZORBAX Rx-SIL
ZORBAX Rx-SILEclipseStableBond
ZORBAX SIL
10
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ZORBAX
C18 C8
C3CN
C8C18
C8
pH
(THF)
200 nm
pH
pH
pH pH pH
2 4
pH
HPLC
/
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11

USP
(USP) USP USP L1
L1L7 L11 (RRHT)
USP
USP (µm) (Å)
L1
1.5 10 µm
1.8, 3.5, 5, 7
ZORBAX Eclipse Plus C18
ZORBAX Eclipse Plus XDB-C18
ZORBAX StableBond SB-C18
ZORBAX Extend-C18
ZORBAX Rx-C18
ZORBAX ODS
ZORBAX ODS Classic
L3 5 10 µm ZORBAX Sil
ZORBAX Rx-Sil
L7
L8
L10
L11
L13
L14
L20
L33
L35
L56
L57
1.5 10 µm
10 µm
3
10 µm
1.5
10 µm
3 10 µm
10 µm
3 10 µm
4,000 400,000
pH
3 10 µm
5 µm 120
ZORBAX Eclipse Plus C8
ZORBAX Eclipse XDB-C8
ZORBAX SB-C8
ZORBAX Rx-C8
ZORBAX C8
3, 5
5
5
5
1.8, 3.5, 5, 7
3.5, 5
3, 5
95
80
80
80
80
70
70
70
80
95
80
80
80
70
ZORBAX NH2 5 70
ZORBAX Eclipse XDB-CN
ZORBAX SB-CN
ZORBAX CN
ZORBAX Eclipse XDB-Phenyl
ZORBAX SB-Phenyl
ZORBAX
3.5, 5
3.5, 5
3, 5
3.5, 5
1.8, 3.5
5
80
80
70
80
80
70
ZORBAX TMS 5 70
ZORBAX SAX 5 70
ZORBAX GF-250 4 150
ZORBAX GF-250 4 150
ZORBAX GF-250 4
6
150
ZORBAX SB-C3 3.5, 5 80
Ultron ES-OVM 5 120
12
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ZORBAX
pH 1-12
pH pH
pH pH
pH pH
1 pH pH
pH pH pH
1HPLC pH
pKa pH
pKa 6.5
pH < 3 - A pH 7 - B pH > 9 - C
pH RP-HPLC
/
pH
pH
LC/MS pH
(TFA)
pKa pH
pH
SiOH pH 4-5
SiO
TEA
Rx-SIL
Rx-SIL
pH
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13

pH (pH 2-3)
pH
pH 2-3 pH
pH
pH pH
20-50 mM
(pH 2-3) LC/MS pH
LC/MS
pH
ZORBAX Eclipse Plus
ZORBAX Eclipse Plus C18 C8 pH Eclipse
Plus Eclipse
Eclipse Plus pH 2-9 pH
2
14
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ZORBAX
pH
pH Eclipse Plus Eclipse
XDB C18C8 StableBond
SB-C18SB-C8SB-PhenylSB-CNSB-C3 SB-Aq
pH pH
pH 1-2 StableBond pH
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15

pH (4-9)
ZORBAX Eclipse Plus
pH pH
Eclipse Plus C18 pH
Eclipse Plus pH 9 pH
- pH
pH 6-9
pH
pH
pH Eclipse Plus C18 pH=7
pH 6.1-8.1
pH 3.8-5.8 LC/MS
ZORBAX Eclipse XDB-PhenylCN Bonus-RP
pH
pH
Eclipse XDB-PhenylEclipse XDB-CN Bonus-RP
Bonus-RP
100%
16
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ZORBAX
pH 3
2a
Eclipse XDB 60°C
Stablebond
80-90°C
pH 7
pH 9
1
ZORBAX Eclipse Plus C18 C8
• pH 2.5 (2-3) 20-50 mM
• T=30°C 60°C
• 0.5 < k < 20
2
5% (v/v)
3
•
•
0.5 < k < 20
• 2
4
•
Eclipse XDB-C18 C8
• SB-CN AQ Bonus-RP
1
5
• ZORBAX Eclipse Plus C18 C8
• pH 7 (6-9)20-50 mM
• T = 30°C 40°C
• 0.5 < k < 20
6
5% (v/v)
7
•
• 0.5 < k < 20
• 6
8
• ZORBAX Eclipse
XDB Bonus-RP
• 5
9
• ZORBAX Extend-C18
• pH 10.5 (9-11.5)5-20 mM
10-50 mM
• T = 25°C 40°C
• 0.5 < k < 20
pH pH
1a
ZORBAX SB-C18 SB-C8
• pH 1-3 20-50 mM
• T = 30°C 90°C
• 0.5 < k < 20
1c
5% (v/v)
5c
5% (v/v)
1b
• 10 mM
25-50 mM pH 3
•
0.5 < k < 20
1d
5% (v/v)
1e
0.5 < k < 20
5a
•
10 mM
25-50 mM pH 7
•
0.5 < k < 20
5b
5d
•
• 0.5 < k < 20
10a
10
•
• 0.5 < k < 20
HPLC
17

HPLC
Agilent HPLC
234 4.6 mm
ZORBAX
400 bar
PEEK
1/16 LC
ZORBAX
3.5 µm 1.8 µm
ZORBAX
ZORBAX Prep
LC/MSLC/MS/MS
Eclipse XDB pH 2-9
StableBond pH
2000 psi135 bar13.5 MPa
1/16 LC
21.2 30 mm
18
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ZORBAX
ZORBAX
ZORBAX
pH ZORBAX
• ZORBAX Eclipse Plus HPLC
• ZORBAX Eclipse XDB HPLC
• ZORBAX StableBond HPLC pH
• ZORBAX Rx HPLC pH 9
• ZORBAX Extend-C18 HPLC pH
• ZORBAX Bonus-RP HPLC -
• ZORBAX
• ZORBAX
• ZORBAX HPLC A
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ZORBAX Eclipse Plus LC ...
Eclipse Plus
Eclipse Plus Eclipse
Eclipse Plus
Eclipse Plus 1.83.5 5 µm LC
LC LC Eclipse
1.8 µm 7 µm
20
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ZORBAX
•
•
• pH
•
• HPLC
• 1.8µm Eclipse Plus
pH
ZORBAX Eclipse Plus C18 95Å 160 m 2 /g 60°C 2.0-9.0 8%
ZORBAX Eclipse Plus C8 95Å 160 m 2 /g 60°C 2.0-8.0 8%
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21

Eclipse Plus:
:
Eclipse Plus C18
Ace 5 C18
SunFireC18
XBridgeC18
Eclipse Plus C18
959996-902
4.6 x 100 mm, 5 µm
1
2
:
:
:
:
A60%
B40%
1.0 mL/min
UV 254 nm
5989-4934EN
DiscoveryHS C18
LunaC18 (2)
GeminiC18
XTerra MS C18
1.
2.
0 0.5 1 1.5 2 2.5 3 3.5
min
Eclipse Plus C18 C8 RRHT RR
A
B
Eclipse Plus C18
959941-902
4.6 x 50 mm, 1.8 µm
Eclipse Plus C8
959943-906
4.6 x 50 mm, 3.5 µm
1. Oxybenzone
2. Internal Std.
3. Octylmethoxycinnamate
: : (30:70)
: 2.0 mL/min
: 30°C
: UV 230 nm
100°C
0.45 µm
1.
2.
3.
247 bar
N: 8800
Pw: 0.014 min.
TF: 1.20
N: 11600
Pw: 0.047 min.
TF: 1.06
N: 11800
Pw: 0.093 min
TF: 1.02
0 1 2 3 4
min
N: 5000
Pw: 0.019 min.
TF: 1.09
N: 6600
Pw: 0.037 min.
TF: 0.99 N: 6300
Pw: 0.061 min.
TF: 0.98
22 min.
88 bar
0 1 2 3 4
min
C8 RRHT
C8
22
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ZORBAX
pH 2 pH 7
:
1.
2.
3.
4.
Eclipse Plus C8
959946-906
4.6 x 50 mm, 5 µm
1
:
3
10-60% B/3 min
pH 2.7A 0.1%
B 0.1% fa
pH 7.0A 20 mM
B:
Excedrin
pH 2.7
2
4
0 0.5 1 1.5 2 2.5 3 3.5 m
1
pH 7.0
3
4
2
2 min.
0 0.5 1 1.5 2 2.5 3 3.5 m
Eclipse Plus C18 C8 pH
Eclipse Plus C8 Eclipse Plus C18
A
B
Eclipse Plus C8
959996-906
4.6 x 100 mm, 5 µm
Eclipse Plus C18
959996-902
4.6 x 100 mm, 5 µm
:
:
:
80% 8mM
K 2 HPO 4 pH 7
1.0 mL/min
UV 215 nm
0.05 µg/µL
0.5 µL
3.789
Tf=1.04
0 1 2 3 4 5 6 7 8 9 min
5.777
Tf=1.00
0 1 2 3 4 5 6 7 8 9 min
C8 C18
Eclipse Plus C8 C18
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23

ZORBAX Eclipse Plus
A
XBridge C184.6 x 150mm
5µm
A
1
N = 14000
Tf = 1.25
2 3
4
5
N = 41000
Tf = 1.13
6
B
Eclipse Plus C18
959993-902
4.6 x 150 mm, 5 µm
0 2 4 6 8 10 12 min
B
1
N = 23000
Tf = 1.01
N = 60000
Tf = 1.00
2 3 4 5
6
0 2 4
6
8 10 12
min
: A 0.1%
B 0.1%
: 1.0 mL/min
: 0.0 min 10% B
15 min 30% B
: 40°C
: UV 254 nm
5989-4934EN
1.
2.
3.
4.
5.
6.
Eclipse Plus
A
Eclipse Plus C18
959941-902
4.6 x 50 mm, 1.8 µm
A
Tf = 1.00
O
O N + Cl -
OCH 3
1.
: A50% 8 mM K2HPO4pH 7
B50% ACN
: 1.0 mL/min
:
: UV 254 nm
5989-4934EN
0.4 mg/mL2 uL
0 0.5
OCH 3
1 1.5 2 2.5
min
B
Eclipse Plus C18
959993-902
4.6 x 150 mm, 5 µm
959993-902
B
Tf = 1.06
0 0.5 1 1.5 2 2.5
min
24
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ZORBAX
ZORBAX Eclipse Plus
Eclipse
Plus C18
USP L1
Eclipse
Plus C8
USP L7
(mm)
(µm)
4.6 x 250 5 959990-902 959990-906
4.6 x 150 5 959993-902 959993-906
4.6 x 100 5 959996-902 959996-906
4.6 x 50 5 959946-902 959946-906
4.6 x 150 3.5 959963-902 959963-906
4.6 x 100 3.5 959961-902 959961-906
4.6 x 75 3.5 959933-902 959933-906
4.6 x 50 3.5 959943-902 959943-906
4.6 x 30 3.5 959936-902 959936-906
600 bar 4.6 x 150 1.8 959994-902
600 bar 4.6 x 100 1.8 959964-902 959964-906
600 bar 4.6 x 50 1.8 959941-902 959941-906
600 bar 4.6 x 30 1.8 959931-902 959931-906
3.0 x 150 5 959993-302 959993-306
3.0 x 150 3.5 959963-302 959963-306
3.0 x 100 3.5 959961-302 959961-306
600 bar 3.0 x 100 1.8 959964-302 959964-306
600 bar 3.0 x 50 1.8 959941-302 959941-306
2.1 x 150 5 959701-902 959701-906
2.1 x 50 5 959746-902 959746-906
2.1 x 150 3.5 959763-902 959763-906
2.1 x 100 3.5 959793-902 959793-906
2.1 x 50 3.5 959743-902 959743-906
2.1 x 30 3.5 959733-902 959733-906
600 bar 2.1 x 100 1.8 959764-902 959764-906
600 bar 2.1 x 50 1.8 959741-902 959741-906
600 bar 2.1 x 30 1.8 959731-902 959731-906
4/ 4.6 x 12.5 5 820950-936 820950-937
4/ 2.1 x 12.5 5 821125-936 821125-937
820888-901 820888-901
www.agilent.com/chem/store
25

O
O
O
CH 3
Si
CH 3
CH 3
Si CH 3
CH 3
CH 3
Si
CH 3
ZORBAX Eclipse XDB
pH (pH 2-9)
1.8 µm 7 µm
CH 3
O Si CH 3
CH 3
CH 3
O Si
CH 3
Eclipse XDB
Agilent ZORBAX Eclipse XDB C18C8
pH (pH 2-9)
Eclipse XDB pH (2-3)
pH (6-8) pH
Eclipse XDB
pH 2-9 1.83.5
5 7 µm Eclipse XDB
C18
pH
ZORBAX Eclipse XDB-C18 80Å 180 m 2 /g 60°C 2.0-9.0 10%
ZORBAX Eclipse XDB-C8 80Å 180 m 2 /g 60°C 2.0-9.0 7.6%
ZORBAX Eclipse XDB- 80Å 180 m 2 /g 60°C 2.0-9.0 7.2%
Phenyl
ZORBAX Eclipse XDB-CN 80Å 180 m 2 /g 60°C 2.0-8.0 4.3%
26
www.agilent.com/chem/store

ZORBAX
ZORBAX Eclipse XDB pH
: Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm
: 1.5 mL/min
: 40°C
A
:
pH 3.0 75% 25 mM
pH 3.025%
B
:
pH 7.0 90% 20 mM
pH 7.0
10% ACN
1.
2.
3.
4.
5.
1.
2. N-
3. N-
ZORBAX Eclipse XDB pH pH 2-9
pH 3 60°C
: ZORBAX SB-C8
883975-906
4.6 x 150 mm, 5 µm
: Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm
:
70% 50 mM NaAc-HClpH 3.0
30% ACN
65%
35%
: 1.0 mL/min
: 60°C
:
ZORBAX SB-C8
P/N 883975-906
ZORBAX Eclipse XDB-C8
P/N 993967-906
Competitive C
Eclipse XDB pH pH Eclipse pH 3 SB-C8
60°C pH 3 12,000
(amitriptyline) Eclipse XDB
www.agilent.com/chem/store
27

pH 7.0
: Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm
: 60% ACN
40% 250 mM pH 7.0
: 1.5 mL/min
: 60°C
:
1.
2.
3.
4.
5.
Rx-Sil sil-gel- pH 7
(60°C) (250 mM)-
Eclipse XDB StableBond
A
Eclipse XDB-C8
966967-906
4.6 x 75 mm, 3.5 µm
B
ZORBAX Rx/SB-C8
866953-906
4.6 x 75 mm, 3.5 µm
: 70% 25 mM NaH2PO4pH 3.0
30%
: 1.0 mL/min
: 35°C
1.
2.
3.
Time (min)
Time (min)
Eclipse XDB StableBond
28
www.agilent.com/chem/store

ZORBAX
Eclipse XDB
A
B
C
Eclipse XDB-Phenyl
963967-912
4.6 x 150 mm, 3.5 µm
Eclipse XDB-C8
963967-906
4.6 x 150 mm, 3.5 µm
Eclipse XDB-C18
963967-902
4.6 x 150 mm, 3.5 µm
: 15% :85%
: 1.0 mL/min
: 35°C
:
1.
2.
3.
-O
Eclipse XDB C18C8
Eclipse XDB-Phenyl
Eclipse XDB-C8
:
Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm
: 85% 25 mM
Na 2 HPO 4 pH 7:15%
: 1.0 mL/min
: 35°C
:
1.
2.
3. Cefoxatime
4.
5.
Eclipse XDB-C8
A
Eclipse XDB-C18
993967-902
4.6 x 150 mm, 5 µm
1.
2.
3.
4.
5.
6.
B
C
Eclipse XDB-CN
993967-905
4.6 x 150 mm, 5 µm
Eclipse XDB-C18
993967-902
4.6 x 150 mm, 5 µm
: A. 60:40 :
B. 60:40 :
C. 77:23 :
: 1.0 mL/min
: 25°C
:
Eclipse XDB-C18 Eclipse XDB-CN
www.agilent.com/chem/store
29

ZORBAX Eclipse XDB
(mm)
(µm)
XDB-C18
USP L1
XDB-C8
USP L7
XDB-Phenyl
USP L11
XDB-CN
USP L10
(, 400 bar)
9.4 x 250 5 990967-202 990967-206
4.6 x 250 5 990967-902 990967-906 990967-912 990967-905
4.6 x 150 5 993967-902 993967-906 993967-912 993967-905
4.6 x 50 5 946975-902 946975-906
4.6 x 150 3.5 963967-902 963967-906 963967-912 963967-905
4.6 x 100 3.5 961967-902 961967-906 961967-905
4.6 x 75 3.5 966967-902 966967-906 966967-912 966967-905
4.6 x 50 3.5 935967-902 935967-906 935967-912
600 bar 4.6 x 100 1.8 928975-902 928975-906
600 bar 4.6 x 50 1.8 927975-902 927975-906
600 bar 4.6 x 30 1.8 924975-902 924975-906
600 bar 4.6 x 20 1.8 926975-902 926975-906
3.0 x 250 5 990967-302 990967-306 990967-312 990967-305
3.0 x 150 5 993967-302 993967-306 993967-312 993967-305
3.0 x 150 3.5 963954-302 963954-306 963954-312 963954-305
3.0 x 100 3.5 961967-302 961967-306 961967-312
3.0 x 75 3.5 966954-302
600 bar 3.0 x 100 1.8 928975-302 928975-306
600 bar 3.0 x 50 1.8 927975-302 927975-306
600 bar 3.0 x 30 1.8 924975-302 924975-306
600 bar 3.0 x 20 1.8 926975-302 926975-306
2.1 x 150 5 993700-902 993700-906 993700-912 993700-905
2.1 x 50 5 960967-902 960967-906 960967-912 960967-905
* 2.1 x 150 3.5 930990-902 930990-906
* 2.1 x 100 3.5 961753-902 961753-906 961753-905
* 2.1 x 75 3.5 966735-902
* 2.1 x 50 3.5 971700-902 971700-906
600 bar** 2.1 x 100 1.8 928700-902 928700-906
600 bar** 2.1 x 50 1.8 927700-902 927700-906
600bar** 2.1 x 30 1.8 924700-902 924700-906
600 bar** 2.1 x 20 1.8 926700-902 926700-906
* 1.0 x 150 3.5 963600-902 963600-906
* 1.0 x 50 3.5 965600-902 965600-906
* 1.0 x 30 3.5 961600-902 961600-906
3/ 1.0 x 17 5 5185-5921 5185-5921
4/ 4.6 x 12.5 5 820950-925 820950-926 820950-927 820950-935
4/ 2.1 x 12.5 5 821125-926 821125-926 821125-926 821125-935
820888-901 820888-901 820888-901 820888-901
*RR 3.5 µm
**RRHT 1.8 µm
30
www.agilent.com/chem/store

ZORBAX
ZORBAX Eclipse XDB
(mm)
(µm)
XDB-C18
USP L1
XDB-C8
USP L7
(PrepHT) ( 820400-901)
PrepHT 21.2 x 250 7 977250-102 977250-106
PrepHT 21.2 x 150 7 977150-102 977150-106
PrepHT 21.2 x 150 5 970150-902 970150-906
PrepHT 21.2 x 100 5 970100-902 970100-906
PrepHT 21.2 x 50 5 970050-902 970050-906
PrepHT 17 x 7.5 5 820212-925 820212-926
820444-901 820444-901
PrepHT 2/ 820400-901 820400-901
XDB-Phenyl
USP L11
XDB-CN
USP L10
(mm)
( 5021-1845)
4.6 x 250 5 7995118-585 7995108-585
4.6 x 150 5 7995118-595 7995108-595
4.6 x 75 3.5 7995118-344 7995108-344
3.0 x 75 3.5 7995230-344
10/ 4.0 x 4 5 7995118-504 7995118-504
5021-1845 5021-1845
(, 400 bar)
4.6 x 50 1.8 922975-902 922975-906
3/ 4.6 x 50 1.8 922975-932
(µm)
XDB-C18
USP L1
2.1 x 50 1.8 922700-902
3/ 2.1 x 50 1.8 922700-932
XDB-C8
USP L7
31

ZORBAX Eclipse XDB
(mm)
(µm)
XDB-C18
USP L1
XDB-C8
USP L7
( 820555-901)
4.6 x 30 3.5 933975-902 933975-906
3/ 4.6 x 30 3.5 933975-932 933975-936
4.6 x 15 3.5 931975-902 931975-906
3/ 4.6 x 15 3.5 931975-932 931975-936
2.1 x 30 3.5 973700-902 973700-906
3/ 2.1 x 30 3.5 973700-932 973700-936
2.1 x 15 3.5 975700-902 975700-906
3/ 2.1 x 15 3.5 975700-932 975700-936
4.6 x 50 1.8 925975-902
3/ 4.6 x 50 1.8 925975-932
4.6 x 30 1.8 923975-902
3/ 4.6 x 30 1.8 923975-932
4.6 x 15 1.8 921975-902
3/ 4.6 x 15 1.8 921975-932
2.1 x 50 1.8 925700-902
3/ 2.1 x 50 1.8 925700-932
2.1 x 30 1.8 923700-902
3/ 2.1 x 30 1.8 923700-932
2.1 x 15 1.8 921700-902
3/ 2.1 x 15 1.8 921700-932
820555-901 820555-901
0.5 x 250 5 5064-8286
0.5 x 150 5 5064-8287
0.5 x 150 3.5 5064-8288
0.5 x 35 3.5 5064-8298
0.3 x 250 5 5064-8269
0.3 x 150 5 5064-8291
0.3 x 150 3.5 5064-8271
0.5 x 35 5 5064-8296
0.3 x 35 5 5064-8297
32
www.agilent.com/chem/store

ZORBAX
ZORBAX 80Å StableBond
pH pH 1
pH
SB-C18SB-C8SB-CNSB-PhenylSB-C3 SB-
Aq B
Agilent ZORBAX StableBond
(SB-C18) SB-C8SB-C3SB-PhenylSB-CN SB-Aq
pH
StableBond
StableBond pH ZORBAX StableBond
O
OH
O
OH
R
Si
R
R
Si
R
R
R 1
R 1
* pH *
ZORBAX 80Å 180 m 2 /g 90°C 1.0-8.0 10%
SB-C18
ZORBAX 80Å 180 m 2 /g 80°C 1.0-8.0 5.5%
SB-C8
ZORBAX 80Å 180 m 2 /g 80°C 1.0-8.0 4%
SB-C3
ZORBAX 80Å 180 m 2 /g 80°C 1.0-8.0 5.5%
SB-Phenyl
ZORBAX 80Å 180 m 2 /g 80°C 1.0-8.0 4%
SB-CN
ZORBAX 80Å 180 m 2 /g 80°C 1.0-8.0
SB-Aq
*StableBond pH pH 6-8

StableBond SB-C18 pH pH 0.890°C
: ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
: ZORBAX Rx-C18
883967-902
4.6 x 150 mm, 5 µm
: 50% /50%
1.0%
: 90°C
Relative Retention
Stablebond SB-C18
-C18
ZORBAX Rx-C18
-C18
A-C18
B-C18
C-C18
D-C18
E-C18
Column Volumes of Mobile Phase Purge
PH (0.8)
(90°C) StableBond SB-C18 ZORBAX Rx-C18
StableBond SB-C18
ZORBAX SB-CN pH (pH 2.050°C)
:
ZORBAX SB-CN
883975-905
4.6 x 150 mm, 5 µm
% k Remaining
ZORBAX StableBond SB-CN StableBond
pH CN
Column Volumes
SB-CN
A ZORBAX SB-C18
866953-902
4.6 x 75 mm, 3.5 µm
B ZORBAX SB-CN
866953-905
4.6 x 75 mm, 3.5 µm
: 30%
70% 25mM NaH 2 PO 4 pH 2.5
: 1.0 mL/min
: 35°C
1.
2.
3.
4.
5.
Time (min)
Time (min)
SB-CN 50%
34
www.agilent.com/chem/store

ZORBAX
RRHT
mAU
450
400
350
300
250
200
150
100
50
0
0
1
2
1
1
2
1 2 3
2
4
3
5
4
3
3
1 2 3 4 5
: (40%):
60% pH=2.4 25mM NaH 2 PO 4
: 1.85 mL/min
5
3, 4 & 5
1.
2.
3.
4.
5.
4
5
5
4
A SB-C18
827975-902
4.6 x 50 mm, 1.8 µm
B SB-C8
827975-906
4.6 x 50 mm, 1.8 µm
C ZORBAX SB-Phenyl
827975-912
4.6 x 50 mm, 1.8 µm
D ZORBAX SB-CN
827975-905
4.6 x 50 mm, 1.8 µm
min
DAD254 nm
LC Agilent 1200 RR LC
1 µL
StableBond 5 SB-Aq
A
ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
D
ZORBAX SB-Phenyl
883975-912
4.6 x 150 mm, 5 µm
B
ZORBAX SB-C8
883975-906
4.6 x 150 mm, 5 µm
E
ZORBAX SB-CN
883975-905
4.6 x 150 mm, 5 µm
C
ZORBAX SB-C3
883975-909
4.6 x 150 mm, 5 µm
SB-C3 StableBond
SB-C3 Rx-SIL
: 18.8 min 0-100% B
A50 mM NaH 2 PO 4
pH 2.595% / 5%
B50 mM NaH 2 PO 4
pH 2.5 47% / 53%
: 1.0 mL/min
: 26°C
:
:
254 nm
1.
2.
3. d-
4.
5.
www.agilent.com/chem/store
35

ZORBAX 80Å StableBond
(mm)
(µm)
SB-C18
USP L1
SB-C8
USP L7
SB-CN
USP L10
SB-C3
USP L56
SB-Phenyl
USP L11
SB-Aq
(, 400 bar)
9.4 x 250 5 880975-202 880967-201 880975-205 880975-209 880975-212
9.4 x 150 5 883975-202
9.4 x 100 5 884975-202
9.4 x 50 5 846975-202
4.6 x 250 5 880975-902 880975-906 880975-905 880975-909 880975-912 880975-914
4.6 x 150 5 883975-902 883975-906 883975-905 883975-909 883975-912 883975-914
4.6 x 50 5 846975-902 846975-906 846975-914
4.6 x 250 3.5 884950-567
4.6 x 150 3.5 863953-902 863953-906 863953-905 863953-912 863953-914
4.6 x 100 3.5 861953-902 861953-906 861953-905 861953-912 861953-914
4.6 x 75 3.5 866953-902 866953-906 866953-905 866953-912 866953-914
4.6 x 50 3.5 835975-902 835975-906 835975-905 835975-912 835975-914
4.6 x 150 1.8 829975-902 829975-906 829975-905 829975-912
600 bar
4.6 x 100 1.8 828975-902 828975-906 828975-905 828975-912 828975-914
600 bar
4.6 x 50 1.8 827975-902 827975-906 827975-905 827975-912 827975-914
600 bar
4.6 x 30 1.8 824975-902 824975-906 824975-905 824975-912 824975-914
600 bar
4.6 x 20 1.8 826975-902 826975-906
600 bar
3.0 x 250 5 880975-302 880975-306 880975-305 880975-309 880975-312 880975-314
3.0 x 150 5 883975-302 883975-306 883975-305 883975-309 883975-312 883975-314
3.0 x 150 3.5 863954-302 863954-306 863954-305 863954-312 863954-314
3.0 x 100 3.5 861954-302 861954-306 861954-305 861954-309 861954-312 861954-314
3.0 x 150 1.8 829975-302 829975-306 829975-305 829975-312
600 bar
3.0 x 100 1.8 828975-302 828975-306 828975-305 828975-312 828975-314
600 bar
3.0 x 50 1.8 827975-302 827975-306 827975-305 827975-312 827975-314
600 bar
3.0 x 30 1.8 824975-302 824975-306 824975-305
600 bar
3.0 x 20 1.8 826975-302 826975-306
600 bar
2.1 x 150 5 883700-922 883700-906 883700-905 883700-909 883700-912
2.1 x 50 5 860975-902 860975-906 860975-905 860975-909 860975-912 860975-914
* 2.1 x 150 3.5 830990-902 830990-906 830990-914
* 2.1 x 100 3.5 861753-902 861753-906 861753-905 861753-912 861753-914
* 2.1 x 75 3.5 866735-902
* 2.1 x 50 3.5 871700-902 871700-906 871700-914
*RR 3.5 µm
**RRHT 1.8 µm
36
www.agilent.com/chem/store

ZORBAX
ZORBAX 80Å StableBond
SB-C18 SB-C8 SB-CN SB-C3 SB-Phenyl
(mm) (µm) USP L1 USP L7 USP L10 USP L56 USP L11 SB-Aq
2.1 x 150 1.8 820700-902 820700-906 820700-905 820700-912
600 bar**
2.1 x 100 1.8 828700-902 828700-906 828700-905 828700-912 828700-914
600 bar**
2.1 x 50 1.8 827700-902 827700-906 827700-905 827700-912 827700-914
600 bar**
2.1 x 30 1.8 824700-902 824700-906 824700-905 824700-912 824700-914
600 bar**
2.1 x 20 1.8 826700-902 826700-906
600 bar**
* 1.0 x 150 3.5 863600-902 863600-906 863600-905
* 1.0 x 50 3.5 865600-902 865600-906
* 1.0 x 30 3.5 861600-902 861600-906
1.0 x 17 5 5185-5920 5185-5920
3/
2/ 9.4 x 15 7 820675-115 820675-115 820675-124 820675-115
4/ 4.6 x 12.5 5 820950-920 820950-915 820950-916 820950-922 820950-917 820950-933
4/ 2.1 x 12.5 5 821125-915 821125-915 821125-924 821125-924 821125-915 821125-933
9.4 x 15 840140-901 840140-901 840140-901 840140-901 840140-901
820888-901 820888-901 820888-901 820888-901 820888-901 820888-901
(PrepHT) ( 820400-901)
PrepHT 21.2 x 250 7 877250-102 877250-106 877250-105 877250-112 877250-114
PrepHT 21.2 x 150 7 877150-102 877150-106 877150-114
PrepHT 21.2 x 150 5 870150-902 870150-906 870150-914
PrepHT 21.2 x 100 5 870100-902 870100-906 870100-914
PrepHT 21.2 x 50 5 870050-902 870050-906 870050-914
PrepHT 17 x 7.5 5 820212-920 820212-915 820212-915 820212-915 820212-933
2/
820444-901 820444-901 820444-901 820444-901 820444-901 820444-901
PrepHT
2/
*RR 3.5 µm
**RRHT 1.8 µm
820400-901 820400-901 820400-901 820400-901 820400-901 820400-901
( 5021-1845)
4.6 x 250 5 7995218-585 7995208-585
(mm)
(µm)
SB-C18
USP L1
SB-C8
USP L7
4.6 x 150 5 7995218-595 7995208-595
4.6 x 75 3.5 7995218-344 7995208-344
10/ 4.0 x 4 5 7995118-504 7995118-504
5021-1845 5021-1845
www.agilent.com/chem/store
37

ZORBAX 80Å StableBond
(mm)
(µm)
SB-C18
USP L1
SB-C8
USP L7
(, 400 bar)
4.6 x 50 1.8 822975-902 822975-906
3/ 4.6 x 50 1.8 822975-932
2.1 x 50 1.8 822700-902
3/ 2.1 x 50 1.8 822700-932
( 820555-901)
4.6 x 30 3.5 833975-902 833975-906
3/ 4.6 x 30 3.5 833975-932 833975-936
4.6 x 15 3.5 831975-902 831975-906
3/ 4.6 x 15 3.5 831975-932 831975-936
2.1 x 30 3.5 873700-902 873700-906
3/ 2.1 x 30 3.5 873700-932 873700-936
2.1 x 15 3.5 875700-902 875700-906
3/ 2.1 x 15 3.5 875700-932 875700-936
4.6 x 50 1.8 825975-902
3/ 4.6 x 50 1.8 825975-932
4.6 x 30 1.8 823975-902
3/ 4.6 x 30 1.8 823975-932
4.6 x 15 1.8 821975-902
3/ 4.6 x 15 1.8 821975-932
2.1 x 50 1.8 825700-902
3/ 2.1 x 50 1.8 825700-932
2.1 x 30 1.8 823700-902
3/ 2.1 x 30 1.8 823700-932
2.1 x 15 1.8 821700-902
3/ 2.1 x 15 1.8 821700-932
820555-901 820555-901
0.5 x 250 5 5064-8258
0.5 x 150 5 5064-8256
0.5 x 35 5 5064-8254
* 0.5 x 150 3.5 5064-8262
0.5 x 35 3.5 5064-8260
0.3 x 250 5 5064-8257
0.3 x 150 5 5064-8255
0.3 x 35 5 5064-8253
0.3 x 150 3.5 5064-8261
*RR 3.5 µm
**RRHT 1.8 µm
38
www.agilent.com/chem/store

ZORBAX
ZORBAX Rx
Eclipse XDB-C18 StableBond SB-C18 Rx-C18 pH
StableBond SB-C18
12% 10%
pH Rx-C18
Rx-C18 pH 9
Rx-C8 SB-C8
pH
ZORBAX Rx-C18 80Å 180 m 2 /g 60°C 2.0-9.0 12%
ZORBAX Rx-C8 80Å 180 m 2 /g 60°C 1.0-8.0 5.5%
Rx-C18
:
:
:
ZORBAX Rx-C18
880967-302
3.0 x 250 mm, 5 µm
35% :65%
0.5 mL/min
1.
2.
Rx-C18 (USP)
4.6 x 250 mm
3.0 mm Rx-
C18 60%
www.agilent.com/chem/store
39

ZORBAX Rx
Rx-C18 Rx-C8
(mm) (µm) USP L1 USP L7**
9.4 x 250 5 880967-202 880967-201
4.6 x 250 5 880967-902 880967-901
4.6 x 150 5 883967-902 883967-901
4.6 x 150 3.5 863967-902 863953-906
4.6 x 100 3.5 861967-902 861953-906
4.6 x 75 3.5 866967-902 866953-906
3.0 x 250 5 880967-302 880975-306
3.0 x 150 5 883967-302 883975-306
3.0 x 150 3.5 863967-302 863954-306
3.0 x 100 3.5 861967-302 861954-306
2.1 x 150 5 883700-902 883700-906
* 2.1 x 100 3.5 861767-902 861753-906
2/ 9.4 x 15 7 820675-115 820675-115
4/ 4.6 x 12.5 5 820950-914 820950-913
4/ 2.1 x 12.5 5 821125-915 821125-915
9.4 x 15 840140-901 840140-901
820888-901 820888-901
(PrepHT) ( 820400-901)
PrepHT 21.2 x 250 7 877967-102 877250-106
PrepHT 21.2 x 150 7 877150-106
PrepHT 21.2 x 150 5 870150-906
PrepHT 21.2 x 100 5 870100-906
PrepHT 21.2 x 50 5 870050-906
PrepHT 2/ 820212-914 820212-915
820444-901 820444-901
PrepHT 2/ 820400-901 820400-901
*RR 3.5 µm
**Rx-C8 SB-C8
40
www.agilent.com/chem/store

ZORBAX
ZORBAX 80Å Extend-C18
pH pH 11.5
pH
LC/MS
O
Si
C18
ZORBAX Extend-C18 C18-C18
pH pH
pH pH
pH
pH 10.5 LC/MS
pH Extend-C18 pH 2-11.5
Extend-C18
pH
* pH
ZORBAX Extend-C18 80Å 180 m 2 /g 60C 2.0-11.5 12.5%
* pH 8 60°C pH 8-11.5 40°C
O
Si
C18
Silica Support
Extend C-18 C18-C18
www.agilent.com/chem/store
41

Extend-C18 pH
:
:
:
:
:
:
ZORBAX Extend-C18
773450-902
4.6 x 150 mm, 5 µm
pH 7
30% 20 mM Na2HPO4
70%
pH 11
30% 20 mM
70%
1.0 mL/min
254 nm
1.
2.
3.
4.
5.
6.
7.
pH pH
Extend-C18
pH
Time (min)
Time (min)
Extend-C18 pH
:
:
:
:
ZORBAX Extend-C18
773450-902
4.6 x 150 mm, 5 µm
20%
80% 0.1 M pH 10.0
1.0 mL/min
pH
W ZORBAX Extend-C18 pH
Amount of Dissolved Silica (mg)
ZORBAX Extend-C18
W
Volume Eluent (L)
42
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ZORBAX
Extend-C18 pH
: ZORBAX Extend-C18
773450-902
4.6 x 150 mm, 5 µm
: 80% 25 mM NaH2PO4pH 3.0
20%
: 1.0 mL/min
: 35C
: 254 nm
:
1.
2.
3.
4.
Extend-C18 pH
Extend-C18 pH pH
Time (min)
ZORBAX 80Å Extend-C18
(mm)
(, 400 bar)
4.6 x 250 5 770450-902
4.6 x 150 5 773450-902
4.6 x 50 5 746450-902
4.6 x 150 3.5 763953-902
4.6 x 100 3.5 764953-902
4.6 x 75 3.5 766953-902
4.6 x 50 3.5 735953-902
600 bar 4.6 x 100 1.8 728975-902
600 bar 4.6 x 50 1.8 727975-902
400 bar 4.6 x 50 1.8 722975-902
600 bar 4.6 x 30 1.8 724975-902
600 bar 4.6 x 20 1.8 726975-902
3.0 x 250 5 770450-302
3.0 x 150 5 773450-302
3.0 x 150 3.5 763954-302
3.0 x 100 3.5 764953-302
3.0 x 50 3.5 735954-302
600 bar 3.0 x 100 1.8 728975-302
600 bar 3.0 x 50 1.8 727975-302
600 bar 3.0 x 30 1.8 724975-302
600 bar 3.0 x 20 1.8 726975-302
(µm)
www.agilent.com/chem/store
43

ZORBAX 80Å Extend-C18
(mm) (µm)
2.1 x 150 5 773700-902
2.1 x 50 5 760450-902
* 2.1 x 100 3.5 761753-902
* 2.1 x 50 3.5 735700-902
600 bar** 2.1 x 100 1.8 728700-902
600 bar** 2.1 x 50 1.8 727700-902
600 bar** 2.1 x 30 1.8 724700-902
600 bar** 2.1 x 20 1.8 726700-902
* 1.0 x 150 3.5 763600-902
* 1.0 x 50 3.5 765600-902
* 1.0 x 30 3.5 761600-902
3/ 1.0 x 17 5 5185-5923
4/ 4.6 x 12.5 5 820950-930
4/ 2.1 x 12.5 5 821125-930
820888-901
(PrepHT) ( 820400-901)
PrepHT 21.2 x 150 5 770150-902
PrepHT 21.2 x 100 5 770100-902
PrepHT 21.2 x 50 5 770050-902
PrepHT 2/ 820400-901
PrepHT 2/ 17 x 7.5 5 820212-930
820444-901
*RR 3.5 µm
**RRHT 1.8 µm
44
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ZORBAX
ZORBAX Bonus-RP
pH pH
100%
O
CH 3
R 1
Si
R 1
PG
R
Agilent ZORBAX Bonus-RP
pH Bonus-RP
C18 C8
Si CH 3
CH 3
R 1
O Si
R 1
PG
R
CH 3
* pH
ZORBAX Bonus-RP 80Å 180 m 2 /g 60°C 2.0-9.0 9.5%
* pH 8 60°C pH 8-9 40°C
Si CH 3
CH 3
R 1
O Si
R 1
PG
R
Bonus-RP
ZORBAX Bonus-RP
A
ZORBAX Bonus-RP
883668-901
4.6 x 150 mm, 5 µm
B
Eclipse XDB-C8
993967-906
4.6 x 150 mm, 5 µm
: 75% 25 mM pH 6
25%
: 1.0 mL/min
:
: 254 nm
: 3 µL
1.
2.
3.
4.
Time (min)
Bonus-RP
www.agilent.com/chem/store
45

Bonus-RP
: Alkye-C8
4.6 x 150 mm5 µm
: 75% 25 mM NH 4 AcpH 5.5
25%
: 1.5 mL/min
: 40°C
: 254 nm
1.
2.
3.
C8 Bonus-RP
pH
Bonus-RP
TF
1. 1.8
2. 1.7
3. 2.1
Time (min)
Bonus-RP 2
: ZORBAX Bonus-RP
883668-901
4.6 x 150 mm, 5 µm
: 80% 25 mM NH 4 AcpH 5.5
20%
: 1.5 mL/min
: 40°C
: 254 nm
1.
2.
3.
TF
1. 1.1
2. 1.1
3. 1.1
Time (min)
46
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ZORBAX
pH pH ZORBAX Bonus-RP
: ZORBAX Bonus-RP
883668-901
4.6 x 150 mm, 5 µm
: 60% 25 mM
pH 7.0:40% ACN
: 1.5 mL/min
: 23°C
Toluene Plate Height, cm
/
Bonus-RP
Column Volume of Purge
Bonus-RP pH 7 10,000
-C18/Bonus-RP
:
:
:
:
ZORBAX Bonus-RP
883668-901
4.6 x 150 mm, 5 µm
50%
50% 0.1%
80%
20%
1.0 mL/min
60°C
23°C
Toluene Plate Height, cm
-C18/
Bonus-RP
Column Volume of Purge
pH
www.agilent.com/chem/store
47

ZORBAX Bonus-RP
(mm)
(, 400 bar)
4.6 x 250 5 880668-901
4.6 x 150 5 883668-901
4.6 x 150 3.5 863668-901
4.6 x 100 3.5 864668-901
4.6 x 75 3.5 866668-901
3.0 x 250 5 880668-301
3.0 x 150 5 883668-301
3.0 x 150 3.5 863668-301
3.0 x 100 3.5 864668-301
2.1 x 150 5 883725-901
2.1 x 50 5 861971-901
* 2.1 x 150 3.5 863700-901
* 2.1 x 100 3.5 861768-901
* 2.1 x 50 3.5 861700-901
* 1.0 x 150 3.5 863608-901
* 1.0 x 50 3.5 865608-901
* 1.0 x 30 3.5 861608-901
3/ 1.0 x 17 5 5185-5922
4/ 4.6 x 12.5 5 820950-928
4/ 2.1 x 12.5 5 821125-928
820888-901
(PrepHT) ( 820400-901)
PrepHT 21.2 x 250 7 878250-101
*RR 3.5 µm
(µm)
PrepHT 21.2 x 150 7 878150-101
PrepHT 21.2 x 150 5 868150-901
PrepHT 21.2 x 100 5 868100-901
PrepHT 21.2 x 50 5 868050-901
PrepHT 2/ 820400-901
PrepHT 2/ 17 x 7.5 5 820212-928
820444-901
48
www.agilent.com/chem/store

ZORBAX
ZORBAX
pH 2-9
Eclipse pH StableBond
pH (1-11.5) pH
pH
ZORBAX
StableBond
4.6 x 150 mm 5 µm SB-C18SB-CN
SB-Phenyl
StableBond
4.6 x 75 mm 3.5 µm SB-C18SB-CN
Eclipse XDB
4.6 x 150 mm 5 µm XDB-C18XDB-C8
XDB-Phenyl
Eclipse XDB
4.6 x 75 mm 3.5 µm XDB-C18XDB-C8
pH
4.6 x 150 mm 5 µm SB-C18XDB-C18
Extend-C18
pH
4.6 x 75 mm 3.5 µm SB-C18XDB-C18
Extend-C18
4.6 x 150 mm 5 µm SB-AqBonus RP
SB-C18
4.6 x 75 mm 3.5 µm SB-AqBonus RP
SB-C18
5183-4624
5183-4625
5183-4626
5183-4627
5185-5807
5185-5808
5185-5809
5185-5810
www.agilent.com/chem/store
49

ZORBAX
ZORBAX HPLC
www.agilent.com/chem/lccustom
1-2
1 899999-888
2
3
4
5 (302) 993-5354 custom_columns@agilent.com
1-2
3
50
www.agilent.com/chem/store

ZORBAX
ZORBAX
ZORBAX A
(mm)
(µm)
ODS (C18)
USP L1
C8
USP L7
USP L11
USP L10
TMS
USP L13
(, 400 bar)
9.4 x 250 5 880952-202 880952-206
4.6 x 250 5 880952-702 880952-706 880952-712 884950-507 880952-710
4.6 x 250 5 884950-543
4.6 x 150 5 883952-702 883952-706 883952-712 884950-526 883952-710
3.0 x 250 5 880952-302
3.0 x 150 5 883952-302
()
2/ 9.4 x 15 7 820675-115 820675-115 820675-115 820675-124
4/ 4.6 x 12.5 5 820950-902 820950-906 820950-912 820950-905 820950-924
9.4 x 15 840140-901 840140-901 840140-901 840140-901 840140-901
820888-901 820888-901 820888-901 820888-901 820888-901
(PrepHT) ( 820400-901)
PrepHT 21.2 x 250 7 877952-102 877952-106 877952-105
PrepHT 2/ 820400-901 820400-901 820400-901
www.agilent.com/chem/store
51

Zorbax
ZORBAX Rx-SIL
(>99.995%)
ZORBAX-SIL
ZORBAX Rx-SIL
HILIC
ZORBAX Eclipse XDB-CN
Rx-SIL
ZORBAX Rx-SIL
ZORBAX CN
ZORBAX SIL
ZORBAX SIL
ZORBAX NH2
- ZORBAX SIL
HPLC
A D
52
www.agilent.com/chem/store

ZORBAX
pH
ZORBAX Rx-SIL 80Å 180 m 2 /g 0-8.0
ZORBAX Eclipse XDB-CN 80Å 180 m 2 /g 2.0-8.0 4.3%
ZORBAX SIL 70Å 300 m 2 /g 0-8.0
ZORBAX CN 70Å 300 m 2 /g 2.0-7.0 7%
ZORBAX NH 2 70Å 300 m 2 /g 2.0-7.0 4%
ZORBAX CN ZORBAX CN
: ZORBAX CN
880952-705
4.6 x 250 mm, 5 µm
: A
B 2-/ (50/50)
: 2 mL/min
: 10 min. B 2-20% 20%
: 50°C
:
278 nm
(n = 10)
0 5 10
15 20
Time (min)
www.agilent.com/chem/store
53

Zorbax
(mm)
(µm)
XDB-CN
USP L10
Rx-SIL
(, 400 bar)
9.4 x 250 5 880975-201 880952-201 880952-205 880952-208
4.6 x 250 5 880975-901 880952-701 880952-705 880952-708 840300-908 990967-905*
4.6 x 150 5 883975-901 883952-701 883952-705 883952-708 843300-908 993967-905*
2.1 x 150 5 883700-901 993700-905*
2.1 x 50 5 860700-708
()
2/
9.4 x 15 5 820675-119 820675-119 820675-111 820675-111
4/
4/
4.6 x 12.5 5 820950-919 820950-901 820950-905 820950-908 820950-908 820950-935
2.1 x 12.5 5 821125-919 821125-935
9.4 x 15 840140-901 840140-901 840140-901 840140-901
820888-901 820888-901 820888-901 820888-901 820888-901
820888-901
(PrepHT) ( 820400-901)
PrepHT 21.2 x 250 7 877250-101 877952-101
PrepHT
21.2 x 250 7 877952-105 877952-108
PrepHT 2/ 820400-901 820400-901 820400-901 820400-901
PrepHT
2/
17 x 7.5 5 820212-919
820444-901
*
54
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ZORBAX (3.5 µm) (1.8 µm)
Eclipse XDBStableBond Eclipse
Plus RR RRHT
ZORBAX 4.6 mm 50%
HPLC LC/MS
ZORBAX HPLC 2.1 mm
5
ZORBAX
ZORBAX
Ultron
www.agilent.com/chem/store
55

ZORBAX
(RR)
ZORBAX
600 bar RRHT Agilent
1200 95%
1.8 µm
ZORBAX LC
RRHT
LC
, (RR)
(RRHT)
ZORBAX 1.8 µm Agilent 1200
... ...
20
100
HPLC 30-40%
56
www.agilent.com/chem/store

ZORBAX 1.8 µm
1.8 µm [600 bar]
2 25%
95%
HPLC
80
50 mm
ZORBAX (1.8 µm) 1.8 µm
250 mm
95% 80 RRHT ZORBAX
Eclipse Plus ZORBAX (Eclipse XDBStableBondExtend)
3.5 µm
1.8 µm
HT HPLC
600 bar Agilent 1200
RRHT-1100 LC Agilent 1100
www.agilent.com/chem/store
57

1100
Agilent 1100 RRHT LC
1100
(VWD) 4.6 x 50 mm1.8 µm (3) 5188-5323
VWD 5 µl
DAD SL
DAD (MWD)
4.6 x 50 mm1.8 µm (2) 5188-5324
DAD 5 µl
2.1 x 50 mm1.8 µm (2) 5188-5328
DAD 1.7 µl
LC/MS
:
:
:
:
: 50°C
:
Eclipse XDB-C18
924975-302
3.0 x 30 mm, 1.8 µm
A0.1% FAB0.075% FA
18 65% B/2 min
65-88% B/3 min
276 nm
2g
10 mL + 10 µL
10 min 0.45 µm
1 µL
mAU
800
700
600
500
400
300
200
100
0
mAU
350
300
250
200
150
100
50
0
mAU
120
100
80
60
40
20
0
Naringin confirmed by LCMS
Grapefruit Rind
0.5 1 1.5 2 2.5
min
Hesperidin confirmed by LCMS
Lime Rind
0.5 1 1.5 2 2.5
min
Citrus Flavonoids
0.5 1 1.5 2 2.5
min
1.
2.
3.
4.
5.
6.
10-20
58
www.agilent.com/chem/store

20X
:
:
:
:
ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
827700-902, 827700-902
A:H2O
B: ACN
1.2 mL/min, 1.0 mL/min, 2.4 mL/min
0.0 min 50% B
11/1.2/0.4 min 100% B
: 40°C, 40°C, 95°C
: UV 254 nm
: Alkylphenones
A
0
B
0
C
2 4 6 8 10
0.2 0.4 0.6 0.8 1
min
min
1. C3-Alkylphenone
2. C4-Alkylphenone
3. C5-Alkylphenone
4. C6-Alkylphenone
5. C7-Alkylphenone
6. C8-Alkylphenone
7. C9-Alkylphenone
8. C10-Alkylphenone
9. C12-Alkylphenone
0 0.2
0.4 min
:
ZORBAX SB-C18
835975-902
4.6 x 50 mm, 3.5 µm
:
ZORBAX SB-C18
835975-902
4.6 x 50 mm, 3.5 µm
(N)
1. 3476
2. 4585
3. 5673
4. 6180
(N)
1. 6560
2. 8958
3. 11508
4. 12266
:
:
:
:
25% 75%
1.5 mL/min
254 nm
1.
2.
3. 4--
4.
3.5 µm
www.agilent.com/chem/store
59

A
B
C
D
:
:
:
:
Eclipse XDB-C18
990967-902
4.6 x 250 mm, 5 µm
Eclipse XDB-C18
963967-902
4.6 x 150 mm, 3.5 µm
Eclipse XDB-C18
966967-902
4.6 x 75 mm, 3.5 µm
Eclipse XDB-C18
925975-902
4.6 x 50 mm, 1.8 µm
73% : 27% 25 mM
pH 7.0
1 mL/min
254 nm
1.
2.
3.
4.
5.
6.
7.
A
25 cm5 µm 11.5 min (3.5 µm)
B C
HT 2.2 min 80%
A
B
:
:
:
Eclipse XDB-C8
928700-906
2.1 x 100 mm, 1.8 µm
Eclipse XDB-C18
961753-902
2.1 x 100 mm, 3.5 µm
A
B
0.5 mL/min
0.0 min 50% B
20.0 min 100% B
: 40°C
: UV 254 nm
:
A
B
mAU
120
100
80
60
40
20
0
0 2 4 6 8 10 12 14 16 18 min
mAU
100
80
60
40
20
1
1
0
0 2 4 6 8 10 12 14 16 18 min
11
11
1.
2. C3-
3. C4-
4. C5-
5. C6-
6. C7-
7. C8-
8. C9-
9. C10-
10. C12-
11. C14-
: SB-C18
827700-902
2.1 x 50 mm, 1.8 µm
: A60%
B40%
: 1 mL/min
: 80°C
: UV 254 nm
: QC
60
www.agilent.com/chem/store

(1.8µm) (3.5µm)
N (3.5 µm)*
N (1.8 µm)
150 21,000 32,500
100 14,000 24,000
75 10,500 17,000**
50 7000 12,000
30 4200 6000
20 3500
15 2100 2500
- N 1/2
* 5 µm HPLC 40%N 4.6 mm
**
ZORBAX 600 bar9000 psi
Eclipse
Plus C18
(mm) (µm) USP L1
600 bar 4.6 x 150 1.8 959994-902
Eclipse
Plus C8
USP L7
Eclipse
XDB-C18
USP L1
Eclipse
XDB-C8
USP L7
Extend-
C18
USP L1
600 bar 4.6 x 100 1.8 959964-902 959964-906 928975-902 928975-906 728975-902
600 bar 4.6 x 50 1.8 959941-902 959941-906 927975-902 927975-906 727975-902
600 bar 4.6 x 30 1.8 959931-902 959931-906 924975-902 924975-906 724975-902
600 bar 4.6 x 20 1.8 926975-902 926975-906 726975-902
600 bar 3.0 x 100 1.8 959964-302 959964-306 928975-302 928975-306 728975-302
600 bar 3.0 x 50 1.8 959941-302 959941-306 927975-302 927975-306 727975-302
600 bar 3.0 x 30 1.8 924975-302 924975-306 724975-302
600 bar 3.0 x 20 1.8 926975-302 926975-306 726975-302
600 bar 2.1 x 100 1.8 959764-902 959764-906 928700-902 928700-906 728700-902
600 bar 2.1 x 50 1.8 959741-902 959741-906 927700-902 927700-906 727700-902
600 bar 2.1 x 30 1.8 959731-902 959731-906 924700-902 924700-906 724700-902
600 bar 2.1 x 20 1.8 926700-902 926700-906 726700-902
600 bar 1.0 x 100 1.8 928600-902 928600-906 728600-902
600 bar 1.0 x 50 1.8 922600-902 922600-906 722600-902
www.agilent.com/chem/store
61

ZORBAX 600 bar9000 psi
SB-C18 SB-C8 SB-Phenyl SB-CN
(mm) (µm) USP L1 USP L7 USP L11 USP L10 SB-Aq
600 bar 4.6 x 150 1.8 829975-902 829975-906 829975-912 829975-905
600 bar 4.6 x 100 1.8 828975-902 828975-906 828975-912 828975-905 828975-914
600 bar 4.6 x 50 1.8 827975-902 827975-906 827975-912 827975-905 828975-914
600 bar 4.6 x 30 1.8 824975-902 824975-906 824975-912 824975-905 824975-914
600 bar 4.6 x 20 1.8 826975-902 826975-906
600 bar 3.0 x 150 1.8 829975-302 829975-306 829975-312 829975-305
600 bar 3.0 x 100 1.8 828975-302 828975-306 828975-912 828975-305 828975-314
600 bar 3.0 x 50 1.8 827975-302 827975-306 827975-912 827975-305 827975-314
600 bar 3.0 x 30 1.8 824975-302 824975-306 824975-305
600 bar 3.0 x 20 1.8 826975-302 826975-306
600 bar 2.1 x 150 1.8 820700-902 820700-906 820700-912 820700-905
600 bar 2.1 x 100 1.8 828700-902 828700-906 828700-912 828700-905 828700-914
600 bar 2.1 x 50 1.8 827700-902 827700-906 827700-912 827700-905 827700-914
600 bar 2.1 x 30 1.8 824700-902 824700-906 824700-912 824700-905 824700-914
600 bar 2.1 x 20 1.8 826700-902 826700-906
600 bar 1.0 x 100 1.8 828600-902 828600-906 828600-905
62

Zorbax 400 bar6000 psi
XDB-C18 XDB-C8 SB-C18 SB-C8 Extend-C18
(mm) (µm) USP L1 USP L7 USP L1 USP L7 USP L1
4.6 x 50 1.8 922975-902 922975-906 822975-902 822975-906 722975-902
3/ 4.6 x 50 1.8 922975-932 822975-932
2.1 x 50 1.8 922700-902 822700-902
3/ 2.1 x 50 1.8 922700-932 822700-932
( 820555-901)
4.6 x 50 1.8 925975-902 825975-902
4.6 x 50 1.8 925975-932 825975-932
3/
2.1 x 50 1.8 925700-902 825700-902
2.1 x 50 1.8 925700-932 825700-932
3/
4.6 x 30 1.8 923975-902 823975-902
4.6 x 30 1.8 923975-932 823975-932
3/
2.1 x 30 1.8 923700-902 823700-902
2.1 x 30 1.8 923700-932 823700-932
3/
4.6 x 15 1.8 921975-902 821975-902
4.6 x 15 1.8 921975-932 821975-932
3/
2.1 x 15 1.8 921700-902 821700-902
2.1 x 15 1.8 921700-932 821700-932
3/
820555-901 820555-901
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63

ZORBAX 3.5 µm
(4.6 mm) (2.1 mm)
5 µm
Agilent ZORBAX 3.5 µm 5 µm
15-150 mm
1-4.6 mmLC/MS
3.5 µm
5 µm ZORBAX
A
ZORBAX Bonus-RP
883668-901
4.6 x 150 mm, 5 µm
B
ZORBAX Bonus-RP
866668-901
4.6 x 75 mm, 3.5 µm
Time (min)
C
ZORBAX Bonus-RP
861700-901
2.1 x 50 mm, 3.5 µm
: 80% 25 mM NH 4 AcpH 5.5
20%
: 1.5 mL/min
1.5 mL/min
0.3 mL/min
: 40°C
: 254 nm
Time (min)
Time (min)
1.
2.
3.
4.
3.5 µm LC/MS
64
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: ZORBAX SB-C18
866953-902
4.6 x 75 mm, 3.5 µm
: A25 mM NaH 2 PO 4
pH 3.0
B
: 2.0 mL/min
: 35°C
: 254 nm
1.
2.
3.
4.
5.
Time (min) Time (min) Time (min)
4.6 x 75 mm3.5 µm
ZORBAX 3.5 µm Eclipse
(mm)
Eclipse
Plus C18
USP L1
Eclipse
Plus C8
USP L7
Eclipse
XDB-C18
USP L1
Eclipse
XDB-C8
USP L7
Eclipse
XDB-Phenyl
USP L11
Eclipse
XDB-CN
USP L10
(µm)
4.6 x 150 3.5 959963-902 959963-906 963967-902 963967-906 963967-912 963967-905
4.6 x 100 3.5 959961-902 959961-906 961967-902 961967-906 961967-905
4.6 x 75 3.5 959933-902 959933-906 966967-902 966967-906 966967-912 966967-905
4.6 x 50 3.5 959943-902 959943-906 935967-902 935967-906 935967-912
4.6 x 30 3.5 959936-902 959936-906 934967-902 934967-906
4.6 x 20 3.5 932967-902 932967-906
3.0 x 150 3.5 959963-302 959963-306 963954-302 963954-306 963954-312 963954-305
3.0 x 100 3.5 959961-302 959961-306 961967-302 961967-306 961967-312
3.0 x 75 3.5 966954-302
* 2.1 x 150 3.5 959763-902 959763-902 930990-902 930990-906
* 2.1 x 100 3.5 959793-902 959793-906 961753-902 961753-906 961753-905
* 2.1 x 75 3.5 966735-902
* 2.1 x 50 3.5 959743-902 959743-906 971700-902 971700-906
* 2.1 x 30 3.5 959733-902 959733-906 974700-902 974700-906
* 2.1 x 20 3.5 972700-902 972700-906
* 1.0 x 150 3.5 963600-902 963600-906
* 1.0 x 50 3.5 965600-902 965600-906
* 1.0 x 30 3.5 961600-902 961600-906
3/
1.0 x 17 5 5185-5921 5185-5921
4/ 4.6 x 12.5 5 820950-936 820950-937 820950-925 820950-926 820950-927 820950-935
4/ 2.1 x 12.5 5 821125-936 821125-937 821125-926 821125-926 821125-926 821125-935
820888-901 820888-901 820888-901 820888-901 820888-901 820888-901
*RR 3.5 µm
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65

ZORBAX 3.5 µm StableBond
SB-C18 SB-C8 SB-CN SB-Phenyl SB-C3
(mm) (µm) USP L1 USP L7 USP L10 USP L11 USP L56 SB-Aq
4.6 x 150 3.5 863953-902 863953-906 863953-905 863953-912 863953-914
4.6 x 100 3.5 861953-902 861953-906 861953-905 861953-912 861953-914
4.6 x 75 3.5 866953-902 866953-906 866953-905 866953-912 866953-914
4.6 x 50 3.5 835975-902 835975-906 835975-905 835975-912 835975-914
4.6 x 30 3.5 834975-902 834975-906
4.6 x 20 3.5 832975-902 832975-906
3.0 x 150 3.5 863954-302 863954-306 863954-305 863954-312 863954-314
3.0 x 100 3.5 861954-302 861954-306 861954-305 861954-312 861954-309 861954-314
* 2.1 x 150 3.5 830990-902 830990-906 830990-914
* 2.1 x 100 3.5 861753-902 861753-906 861753-905 861753-912 861753-914
* 2.1 x 75 3.5 866735-902
* 2.1 x 50 3.5 871700-902 871700-906 871700-914
* 2.1 x 30 3.5 874700-902 874700-906
* 2.1 x 20 3.5 872700-902 872700-906
* 1.0 x 150 3.5 863600-902 863600-906
* 1.0 x 50 3.5 865600-902 865600-906
* 1.0 x 30 3.5 861600-902 861600-906
1.0 x 17 5 5185-5920 5185-5920
3/
4/ 4.6 x 12.5 5 820950-920 820950-915 820950-916 820950-917 820950-922 820950-933
4/ 2.1 x 12.5 5 821125-926 821125-926 821125-924 821125-926 821125-924 821125-933
820888-901 820888-901 820888-901 820888-901 820888-901 820888-901
*RR 3.5 µm
66
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ZORBAX 3.5 µm RxBonus-RP Extend-C18
*RR 3.5 µm
Rx-C18 Rx-C8
Extend-C18
(mm) (µm) USP L1 USP L7 Bonus-RP USP L1
4.6 x 150 3.5 863967-902 863953-906 863668-901 763953-902
4.6 x 100 3.5 861967-902 861953-906 864668-901 764953-902
4.6 x 75 3.5 866967-902 866953-906 866668-901 766953-902
4.6 x 50 3.5 735953-902
3.0 x 150 3.5 863967-302 863954-306 863668-301 763954-302
3.0 x 100 3.5 861967-302 861954-306 864668-301 764953-302
2.1 x 150 3.5 830990-906 863700-901
* 2.1 x 100 3.5 861767-902 861753-906 861768-901 761753-902
* 2.1 x 50 3.5 861700-901 735700-902
* 1.0 x 150 3.5 863608-901 763600-902
* 1.0 x 50 3.5 865608-901 765600-902
* 1.0 x 30 3.5 861608-901 761600-902
3/ 1.0 x 17 5 5185-5922 5185-5923
4/ 4.6 x 12.5 5 820950-914 820950-913 820950-928 820950-930
4/ 2.1 x 12.5 5 821125-915 821125-915 821125-928 821125-930
820888-901 820888-901 820888-901 820888-901
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67

ZORBAX
4.6 mm 60%
S/N 2 3
LC/MS
LC
ZORBAX 3.0 mm 4.6mm
50% LC/MS
0.5 ml/min (APCI) (APPI)
MS 4.6 mm 2 3
HPLC
60%
A
B
:
:
ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
ZORBAX SB-C18
883975-302
3.0 x 150 mm, 5 µm
20% : 80% 0.2 M Na2HPO4
+ 0.1 M pH 2.6
1.
2.
3.
4.
5.
6.
Time (min)
Time (min)
4.6 3.0 mm 50%
68
www.agilent.com/chem/store

A
B
:
:
ZORBAX SB-C18
863953-902
4.6 x 150 mm, 3.5 µm
ZORBAX SB-C18
863954-302
3.0 x 150 mm, 3.5 µm
25% :
75% 0.4%
254 nm
1.
2. 45,7 -
4.6 mm
2-3 HPLC
Time (min)
LC/MS
:
ZORBAX SB-C18
861954-302
3.0 x 100 mm, 3.5 µm
A70% + 0.4%
B30% + 0.4%
0.425 mL/min
AUV 254 nm
B APCI
C
1.
2.
3.
4.
5.
6.
LC/MS MS
APCI 0.5 mL/min
Time (min)
www.agilent.com/chem/store
69

ZORBAX 80Å StableBond
SB-C18 SB-C8 SB-CN SB-C3 SB-Phenyl
(mm) (µm) USP L1 USP L7 USP L10 USP L56 USP L11 SB-Aq
3.0 x 250 5 880975-302 880975-306 880975-305 880975-309 880975-312 880975-314
3.0 x 150 5 883975-302 883975-306 883975-305 883975-309 883975-312 883975-314
3.0 x 150 3.5 863954-302 863954-306 863954-305 863954-309 863954-312 863954-314
3.0 x 100 3.5 861954-302 861954-306 861954-305 861954-309 861954-312 861954-314
ZORBAX 300Å StableBond
300SB-C18 300SB-C8 300SB-CN 300SB-C3
(mm) (µm) USP L1 USP L7 USP L10 USP L56
3.0 x 150 3.5 863974-302 863974-306 863974-309 863974-309
3.0 x 100 3.5 861973-306
ZORBAX 80Å Eclipse XDB
XDB-C18 XDB-C8 XDB-Phenyl XDB-CN
(mm) (µm) USP L1 USP L7 USP L11 USP L10
3.0 x 250 5 990967-302 990967-306 990967-312 990967-305
3.0 x 150 5 993967-302 993967-306 993967-312 993967-905
3.0 x 150 3.5 963954-302 963954-306 963954-312 963954-305
3.0 x 100 3.5 961967-302 961967-306 961967-312
3.0 x 75 3.5 966954-302
ZORBAX 80Å Bonus-RP Rx
Rx-C18 Rx-C8
(mm) (µm) Bonus-RP USP L1 USP L7
3.0 x 250 5 880668-301 880967-302 880975-306
3.0 x 150 5 883668-301 883967-302 883975-306
3.0 x 150 3.5 863668-301 863967-302 863954-306
3.0 x 100 3.5 864668-301 861967-302 861954-306
ZORBAX 80Å Extend-C18
Extend-C18
(mm) (µm) USP L1
3.0 x 250 5 770450-302
3.0 x 150 3.5 763954-302
3.0 x 150 5 773450-302
3.0 x 100 3.5 764953-302
3.0 x 50 3.5 735954-302
70
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ZORBAX 1.0 mm
LC/MS
1 mm 2.1 mm
5
50 µl/
HPLC
400 bar StableBond SB-C18SB-C8300SB-C18
Eclipse XDB-C18 XDB-C8Bonus RPExtend C-18 Poroshell
1.8 µm
ZORBAX 300SB-C18
: ZORBAX 300SB-C18
863630-902
1.0 x 150 mm, 3.5 µm
: 60 min 2-60% B
A0.1%
B0.075% /80%
: 50 µL/min
: 50°C
: 215 nm
2 µL
rhGH
1.0 mm
www.agilent.com/chem/store
71

ZORBAX 1.0 mm
SB-C18 SB-C8 300SB-C18 300SB-C8
(mm)
(µm) USP L1 USP L7 USP L1 USP L7
1.0 x 250 5 861630-902
* 1.0 x 150 3.5 863600-902 863600-906 863630-902 863630-906
* 1.0 x 50 3.5 865600-902 865600-906 865630-902 865630-906
* 1.0 x 30 3.5 861600-902 861600-906
SB-CN
USP L10
** 1.0 x 50 1.8 822600-902 822600-906 822600-905
3/ 1.0 x 17 5 5185-5920 5185-5920 5185-5920 5185-5920
XDB-C18 XDB-C8
Extend-C18
(mm)
(µm) USP L1 USP L7 Bonus-RP USP L1
* 1.0 x 150 3.5 963600-902 963600-906 863608-901 763600-902
* 1.0 x 50 3.5 965600-902 965600-906 865608-901 765600-902
* 1.0 x 30 3.5 961600-902 961600-906 861608-901 761600-902
600 bar** 1.0 x 100 1.8 928600-902 928600-906 728600-902
3/ 1.0 x 17 5 5185-5921 5185-5921 5185-5922 5185-5923
(mm)
(µm)
Poroshell Poroshell
300SB-C18 300SB-C8
Poroshell
300SB-C3
Poroshell
300Extend-C18
1.0 x 75 5 661750-902 661750-906 661750-909 671750-902
3/ 1.0 x 17 5 5185-5968 5185-5968 5185-5968
*RR 3.5 µm
**RRHT 1.8 µm
72
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ZORBAX
LC/MS
0.50.30.1 0.075 mm
80Å 300Å/
Agilent ZORBAX 0.50.3 mm
0.10.075 mm
HPLC
LC/MS
SCX
www.agilent.com/chem/store
73

A
B
C
D
E
F
ZORBAX 300SB-C8
5065-4460
0.3 x 150 mm, 3.5 µm
ZORBAX Eclipse XDB-C18
5064-8291
0.3 x 150 mm, 5 µm
ZORBAX Eclipse XDB-C18
5064-8291
0.3 x 150 mm, 5 µm
ZORBAX SB-C18
5064-8255
0.3 x 150 mm, 5 µm
ZORBAX 300SB-C18
5064-8267
0.3 x 150 mm, 3.5 µm
ZORBAX 300Extend-C18
5065-4464
0.3 x 150 mm, 3.5 µm
: + 0.05% pH = 2.2 = A
+ 0.045% = B
0.5%B/min0 min = 1%B
60 min = 31%B70 min = 50%B
75 min = 85%B
80 min = 85%B81 min = 1%B
110 min = 1%B
: 5.5 µL/min
200-500 µl/min
: 30°C
: 206/10 nm 450/80 nm
0.1 µl
400
300
200
100
0
1. -5 ng/100 nl
2. --20 ng/100 nl
3. 28 ng/100 nl
4. 20 ng/100 nl
5. II20 ng/100 nl
1
1
1
1
1
1
2 3 4 5
1 2 3 4 5
2 3 4 5
2 3 4 5
2 3 4 5
2 3 4 5
2 3 4
0 20 40 60
Time (min)
ZORBAX
5
74
www.agilent.com/chem/store

:
:
:
:
ZORBAX SB-C18
5064-8255
0.3 x 150 mm, 5 µm
ZORBAX SB-C18
5064-8256
0.5 x 150 mm, 5 µm
ZORBAX SB-C18
863600-902
1.0 x 150 mm, 3.5 µm
ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
200 ng
0.3 mm 4.6 mm
100 0.1 mm-0.075 mm
2000
Time (min)
www.agilent.com/chem/store
75

: ZORBAX SB-C18
5064-8256
0.5 x 150 mm, 5 µm
: A75% 0.4%
B25% 0.4%
: 20 µL/min
: 25°C
: 0.1 µL
1.
2.
3.
4.
5.
6.
StableBond-C180.5 x 150 mm 5 µm
(k) 0.8% RSD
(a) 0.4% RSD
Time (min)
76

LC/MS ZORBAX
HPLC
1100/1200
1.
1100
SCX
1100/1200
1100/1200
LC/MSD
1100/1200
2.
1100
SCX
1100/1200
1100/1200
LC/MSD
www.agilent.com/chem/store
77

HPLC 2-D HPLC
:
:
:
:
:
MS :
ZORBAX 300SB-C18
5065-9913
0.3 x 5 mm, 5 µm
ZORBAX 300SB-C18
5065-9911
0.075 x 150 mm, 3.5 µm
: 3% /0.1%
: A = , 0.1% , B = , 0.1%
: 30 µL/min
: 300 nL/min
:
:
6 min = 3%B, 120 min = 60%B,
125 min = 80%B,
130 min = 80% B, 131 min = 3%B,
140 min = 3% B
: Nano ESI, : 5L/min,
.: 225C.
: : 1:35 V, :
115 V, 1:12 V,
2:3.5 V, : 80 V. ICC: on,
: 4, : 150 ms;
60.000, , MS/MS
: 1 - 8 µL
Salt Step Elution: 8ml 10 mM-100 mM
KCI (10 mM ), 125 mM,
150 mM, 200 mM, 300 mM, 500 mM, 1M.
Time (min)
30 mM KCI
60 mM KCI
75 mM KCI
120 mM KCI
(BSA) HPLC
BSA
78
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ZORBAX
Poroshell
Eclipse 300SB-
300Extend Bio-SCX
(mm) (µm) SB-C18 XDB-C18 C18 300SB-C8 300SB-C8 C18 II
0.8 x 50 3.5 5065-9942
0.5 x 250 5 5064-8258 5064-8286 5064-8266
0.5 x 150 5 5064-8256 5064-8287 5064-8264
0.5 x 75 5 5065-4468
0.5 x 35 5 5064-8254 5064-8296 5064-8294
* 0.5 x 35 3.5 5064-8260 5064-8298 5065-4459
0.3 x 250 5 5064-8257 5064-8269 5064-8265
0.3 x 150 5 5064-8255 5064-8291 5064-8263
0.3 x 35 5 5064-8253 5064-8297 5064-8295
0.3 x 35 3.5 5065-9912
* 0.3 x 150 3.5 5064-8261 5064-8271 5064-8267 5065-4460 5065-4464
* 0.3 x 100 3.5 5064-8259 5065-4461 5065-4465
* 0.3 x 75 3.5 5064-8270 5065-4462 5065-4466
* 0.3 x 50 3.5 5064-8300 5065-4463 5065-4467
10/ 5065-4427 5065-4427 5065-4427 5065-4427 5065-4427 5065-4427
*RR 3.5 µm
300SB-C18
(mm) (µm) USP L1
** 0.1 x 150 3.5 5065-9910
** 0.075 x 150 3.5 5065-9911
300SB-C8
USP L7
* 0.075 x 50 3.5 5065-9924 5065-9923
/5/ 0.3 x 5 5 5065-9913 5065-9914
/ 5065-9915 5065-9915
*RR 3.5 µm
www.agilent.com/chem/store
79

ZORBAX PrepHT
ZORBAX
2000 mg
5 7 µm
5000 psi/350 bar
ZORBAX PrepHT
Eclipse XDBStableBondBonus-RP Extend-C18
ZORBAX PrepHT 5 7 µm
ZORBAX 100 ml/min
ZORBAX PrepHT
21.2 mm 150 mm
7 µm
80
www.agilent.com/chem/store

ZORBAX PrepHT
DAD
Vial 1 | 347
Vial 2 | 343
Vial 3 | 389
MSD
1.
2.
3.
0 1 2 3 4 5 6 7
Time (min)
ZORBAX SB-C18 5988-7113EN
>90% 2000 mg
ZORBAX PrepHT 21.2 mm
150 mm 7 µm
ZORBAX SB-C18
(mL/min) (µL)
1 50 x 150 mm 100 2200 0.3 mm
2 21.2 x 150 mm 18 400 0.3 mm 877150-102
3 9.4 x 150 mm 3.5 80 0.3 mm 883975-202
4 4.6 x 150 mm 0.85 2.0 3 mm 883975-902
1100
4.6 mm 50 mm
1
2
3
4
1.
2.
3.
Time (min)
www.agilent.com/chem/store
81

ZORBAX PrepHT StableBond
ZORBAX PrepHT 80ÅStableBond
SB-C18 SB-C8
SB-CN SB-Phenyl
(mm)
(µm) USP L1 USP L7 SB-Aq USP L10 USP L11
PrepHT * 21.2 x 250 7 877250-102 877250-106 877250-114 877250-105 877250-112
21.2 x 150 7 877150-102 877150-106 877150-114
21.2 x 150 5 870150-902 870150-906 870150-914
21.2 x 100 5 870100-902 870100-906 870100-914
21.2 x 50 5 870050-902 870050-906 870050-914
PrepHT 17 x 7.5
2/**
5 820212-920 820212-915 820212-933 820212-933 820212-915
ZORBAX PrepHT 300Å StableBond
300SB-C18 300SB-C8 300SB-C3 300SB-CN
(mm)
(µm) USP L1 USP L7 USP L56 USP L10
PrepHT * 21.2 x 250 7 897250-102 897250-106 897250-109 897250-105
PrepHT
2/**
21.2 x 150 7 897150-102 897150-106 897150-109
21.2 x 150 5 895150-902 895150-906 895150-909
21.2 x 100 5 895100-902 895100-906 895100-909
21.2 x 50 5 895050-902 895050-906 895050-909
17 x 7.5 5 820212-921 820212-918 820212-924 820212-924
* PrepHT 820400-901
** (820444-901) 1
ZORBAX PrepHT Eclipse XDB
XDB-C18 XDB-C8
(mm)
(µm) USP L1 USP L7
PrepHT * 21.2 x 250 7 977250-102 977250-106
PrepHT ,
2/**
21.2 x 150 7 977150-102 977150-106
21.2 x 150 5 970150-902 970150-906
21.2 x 100 5 970100-902 970100-906
21.2 x 50 5 970050-902 970050-906
17 x 7.5 5 820212-925 820212-926
* PrepHT 820400-901
** (820444-901) 1
82
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ZORBAX PrepHT Bonus-RP Extend-C18
(mm) (µm) Bonus-RP
PrepHT * 21.2 x 250 7 878250-101
21.2 x 150 7 878150-101
Extend-C18
USP L1
21.2 x 150 5 868150-901 770150-902
21.2 x 100 5 868100-901 770100-902
21.2 x 50 5 868050-901 770050-902
PrepHT 2/** 17 x 7.5 5 820212-928 820212-930
* PrepHT 820400-901
** (820444-901) 1
ZORBAX PrepHT
ODS (C18) C8
(mm) (µm) USP L1 USP L7 USP L10
PrepHT * 21.2 x 250 7 877952-102 877952-106 877952-105 877952-108 877952-101
* PrepHT 820400-901
ZORBAX PrepHT Rx-SIL
SIL
(mm) (µm) USP L3
PrepHT * 21.2 x 250 7 877250-101
Rx-C18
USP L1
21.2 x 250 7 877967-102
PrepHT 2/** 17 x 7.5 5 820212-919 820212-914
* PrepHT 820400-901
** (820444-901) 1
ZORBAX PrepHT
820444-901
PrepHT 2/ 820400-901
820385-901
www.agilent.com/chem/store
83

4.6 mm 50 mm
21.2 mm pH 10
21.230 50 mm 50-250 mm 5 10 µm
21.2 mm
4.6 mm
C18
pH
C18 100Å 400 m 2 /g 60°C* 2.0-10.0 24%
100Å 400 m 2 /g ** 1.0-8.0 N/A N/A
* pH 8 60°C pH 8-10 40°C
** pH
84
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Agilent Prep C18
: Agilent Prep-C18
443905-902
4.6 x 150 mm, 5 µm
: 50% 0.1% :
50%
: 1 mL/min
10 µL
/
0.5-50 mg/mL
Prep
Prep
A Agilent Prep-C18
443905-902
4.6 x 150 mm, 5 µm
B 443905-102
21.2 x 150 mm, 5 µm
C 413910-302
30 x 150 mm, 10 µm
D 413910-502
50 x 150 mm, 10 µm
:
:
55% :45%
0.7 mL/min
14.87 mL/min
29.77 mL/min
85.37 mL/min
240 nm
:
:
2 µL
42.4 µL
170 µL
488 µL
1.
2. 1mg/mL
Prep-C18
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85

(, 400 bar)
(mm)
(µm) C18
Scalar 4.6 x 250 5 440905-902 440905-901
Scalar 4.6 x 150 5 443905-902 443905-901
Scalar 4.6 x 100 5 449905-902 449905-901
Scalar 4.6 x 50 5 446905-902 446905-901
(PrepHT) ( 820400-901)*
PrepHT 21.2 x 250 10 410910-102 410910-101
PrepHT 21.2 x 150 10 413910-102 413910-101
PrepHT 21.2 x 50 10 446910-102
PrepHT 21.2 x 150 5 443905-102 443905-101
PrepHT 21.2 x 100 5 449905-102 449905-101
PrepHT 21.2 x 50 5 446905-102 446905-101
PrepHT 2/ 820400-901 820400-901
(, 400 bar)
Prep 30 30 x 250 10 410910-302 410910-301
Prep 30 30 x 150 10 413910-302 413910-301
Prep 30 30 x 100 10 419910-302 419910-301
Prep 30 30 x 100 5 449905-302 449905-301
Prep 30 30 x 50 5 446905-302 446905-301
Prep 50 50 x 250 10 410910-502 410910-501
Prep 50 50 x 150 10 413910-502 413910-501
Prep 50 50 x 100 10 419910-502 419910-501
Prep 50 50 x 100 5 449905-502 449905-501
Prep 50 50 x 50 5 446905-502 446905-501
()
PrepHT 2/ 21.2 x 10 10 420212-902 420212-901
820444-901 820444-901
PrepHT 420420-901 420420-901
(1kg) 10 420910-902 420910-901
* PrepHT 820400-901 21.2 mm PrepHT 820444-901 30 mm
420420-901
86
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Ultron
Ultron
ES-OVM ES-Pepsin
HPLC
www.agilent.com/chem/store
87

(Prozac)
: Ultron ES-OVM
702111651
4.6 x 150 mm, 5 µm
: 25:75 (v/v) EtOH / 20 mM KH 2 PO 4 , pH 5.5
(NaOH)
:
: UV (225 nm)
Courtesy of D. S. Risley and V. S. Sharp of Lilly Research Laboratories, Eli Lilly and Co.
0 5 10 15 20 25 30
Retention Time (min)
ULTRON ES-OVM
: Ultron ES-OVM
702111651
4.6 x 150 mm, 5 µm
: 20 mM KH 2 PO 4 (pH 4.6)
: 1 mL/min
: 25C
: 220 nm
H
Cl N H C 2 H
C 5
H 2 N
S
NH
S
O O O O
10 20 T ime (min)
(mm) (µm) ES-OVM ES-Pepsin
10 x 150 5 722111723
4.6 x 250 10 724111653
4.6 x 150 5 702111651 822111651
4.6 x 150 5 702111651A 822111631A
2.0 x 150 5 702111610
4.0 x 10 5 712111630 832111630
88
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ZORBAX
HPLC
HPLC Agilent ZORBAX
ZORBAX 300StableBond
ZORBAX 300Extend pH 10 LC/MS
ZORBAX Poroshell
ZORBAX Eclipse (AAA)
14 7.5cm 24
15cm
ZORBAX GF-250/450
GF-250 GF-450
4,000-900,000
ZORBAX (SAX) (SCX)
Bio-SCX
89

ZORBAX
ZORBAX < 2000
(300Å)
MW < 50 kDA
MW < 1,000 kDA
StableBond 300SB-C8
300 SB
C8
C18 C8
C3C4 CN
Poroshell 300SB-C18
Poroshell 300SB
Poroshell
Poroshell
C18
C8
C3
StableBond 300SB-C8
4.6 x 150mm3.5 5 µm
883995-906
863973-906
A95% H 2
O:5% 0.1%
B5% H 2
O:95% 0.085%
60 min 0-60% B
35-40°C
1 mL/min
Poroshell 300SB-C18
2.1 x 75mm5µm
660750-902
A95% H 2
O:5% 0.1%
B5% H 2
O:95% 0.085%
10 min 0-60% B
35-40°C
2 mL/min
90
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ZORBAX
pH
0.1% 300Å
30-60 minPoroshell
pH ZORBAX 300StableBond
Poroshell 300SB ZORBAX 300Extend-C18
pH6-8 M
5% 6 M
+ /
DMSO 0.1%-1% DMSO
StableBond 300SB - 80°C Poroshell 300SB - 80°C
pH
pH pH
pH pH pH
ZORBAX
300Extend-C18 pH
300Extend-C18
4.6 x 150 mm5 µm
773995-902
A 20 mM NH 4
OH H 2
O
B 20 mM NH 4
OH 80%
30 5-60% B
25-30°C (

pH
pH
MW < 50 kDa
300SB-C8
4.6 x 150 mm, 3.5 µm
863973-906
MW < 1000 kDa
Poroshell 300SB-C18
2.1 x 75 mm, 5 µm
660750-902
MW < 25 kDa
300 Extend-C18
4.6 x 150 mm, 3.5 µm
763973-902
LC/MS
LC/MS
LC/MS LC/MS
LC/MS
MS
LC/MS
LC/MS 2.1 mm
Poroshell
pH
pH
MW < 50 kDa
300SB-C8
2.1 x 150 mm, 3.5 µm
863750-906
MW < 1000 kDa
Poroshell 300SB-C18
1.0 x 75 mm, 5 µm
661750-902
MW < 25 kDa
300Extend-C18
2.1 x 150mm, 3.5µm
763750-902
0.5 mm 0.3 mm
pH 0.1 0.075 mm
LC/MS C18
92
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ZORBAX
pH
pH
MW < 50 kDa
300SB-C18
0.5 x 150 mm, 3.5 µm
5064-8268
MW < 1000 kDa
Poroshell 300SB-C8
0.5 x 74 mm, 5 µm
5065-4468
MW < 25 kDa
300Extend-C18
0.3 x 150 mm, 3.5 µm
5065-4464
/ LC/MS
SCX
0.3 x 35 mm, 5 µm
5065-9912
0.075 x 150 mm, 3.5 µm
5065-9911
pH
pH
LC/MS
0.1-1.0%
1% pH
LC/MS 10-20 mM NH 4
OH pH
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93

R
ZORBAX 300Å StableBond
O
Si
R 1
ZORBAX 300StableBond
OH
R
R
300Å 300StableBond pH
pH LC/MS 300StableBond
C18C8C3
O
Si
R 1
80-90°C 300StableBond 300SB-C18 300SB-C8
OH
R
R
0.30.5 mm 0.075 0.10 mm
LC/MS
O
Si
R 1
R
300StableBond
* pH *
ZORBAX 300SB-C18 300Å 45 m 2 /g 90°C 1.0-8.0 2.8%
ZORBAX 300SB-C8 300Å 45 m 2 /g 90°C 1.0-8.0 1.5%
ZORBAX 300SB-C3 300Å 45 m 2 /g 90°C 1.0-8.0 1.1%
ZORBAX 300SB-CN 300Å 45 m 2 /g 90°C 1.0-8.0 1.2%
*300 StableBond pH pH 6-8

ZORBAX
ZORBAX 300SB-C3 pH
ZORBAX 300SB-C3
883995-909
4.6 x 150 mm, 5 µm
80 min0-100% B
A 0.5% TFA
B0.5% TFA
1- 50% A50% B
1.0 mL/min
60°C
% k Phenyltheptane Remaining
ZORBAX 300 SB-C3
C4
Volume of Mobile Phase (mL)
300SB
A
ZORBAX 300SB-C18
883995-902
4.6 x 150 mm, 5 µm
B
ZORBAX 300SB-C8
883995-906
4.6 x 150 mm, 5 µm
1.
2.
3. C
4.
C
ZORBAX 300SB-C3
883995-909
4.6 x 150 mm, 5 µm
5.
6. CDR
7.
D
ZORBAX 300SB-CN
883995-905
4.6 x 150 mm, 5 µm
8.
9. S-100β
10. S-100α
40 min 25 - 70% B
A 0.1% TFA
B 0.09% TFA 80%
/20%
1.0 mL/min
60°C
3 µg
Time (min)
300SB-C18C8C3 CN
300SB-CN
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95

HPLC UV MS
:
ZORBAX 300SB-C18
5064-8263
0.3 x 150 mm, 5 µm
UV
:
:
:
MS :
50 min 5-55% B 55-57 min 85% B
A 0.1%
B 0.1%
5.5 µL/min
206 nm
LC/MS:ESI
LC/MSD -Vcap 4000 V
7 L/min
250°C
15 psi
50 V
300 ms
3
3 m/z
1.0 V
100 nL
(4 pmol)
UV at 206 nm
% Relative Abundance
MS
Time (min)
ZORBAX 300SB-C18 0.3 mm
UV MS MS
Time (min)
LC/MS ZORBAX
:
ZORBAX 300SB-C18
5065-9911
0.075 x 150 mm, 3.5 µm
Intens.
:
:
:
:
A + 0.1%
B+ 0.1%
600 nl/min
25 min 2% B 52% B
MS
100 fm (1 µl) 8
ZORBAX Nano HPLC 0.075 mm
LC/MS
Time (min)
96
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ZORBAX
ZORBAX 300Å StableBond
(mm)
(, 400 bar)
(µm)
300SB-C18
USP L1
300SB-C8
USP L7
300SB-CN
USP L10
300SB-C3
USP L56
9.4 x 250 5 880995-202 880995-206 880995-205 880995-209
4.6 x 250 5 880995-902 880995-906 880995-905 880995-909
4.6 x 150 5 883995-902 883995-906 883995-905 883995-909
4.6 x 50 5 860950-902 860950-906 860950-905 860950-909
4.6 x 150 3.5 863973-902 863973-906 863973-905 863973-909
4.6 x 100 3.5 861973-902 861973-906
4.6 x 50 3.5 865973-902 865973-906 865973-905 865973-909
3.0 x 150 3.5 863974-302 863974-306 863974-309
3.0 x 100 3.5 861973-306
2.1 x 250 5 881750-902
2.1 x 150 5 883750-902 883750-906 883750-905 883750-909
* 2.1 x 150 5 863750-906
* 2.1 x 100 3.5 861775-902 861775-906
* 2.1 x 50 3.5 865750-902 865750-906
1.0 x 250 5 861630-902
* 1.0 x 150 3.5 863630-902 863630-906
* 1.0 x 50 3.5 865630-902 865630-906
3/ 1.0 x 17 5 5185-5920 5185-5920
2/ 9.4 x 15 7 820675-124 820675-124 820675-124 820675-124
4/ 4.6 x 12.5 5 820950-921 820950-918 820950-923 820950-924
4/ 2.1 x 12.5 5 821125-918 821125-918 821125-924 821125-924
9.4 x 15 840140-901 840140-901 840140-901 840140-901
820888-901 820888-901 820888-901 820888-901
(PrepHT) ( 820400-901)
PrepHT 21.2 x 250 7 897250-102 897250-106 897250-105 897250-109
PrepHT 21.2 x 150 7 897150-102 897150-106 897150-109
PrepHT 21.2 x 150 5 895150-902 895150-906 895150-909
PrepHT 21.2 x 100 5 895100-902 895100-906 895100-909
PrepHT 21.2 x 50 5 895050-902 895050-906 895050-909
PrepHT 2/ 820400-901 820400-901 820400-901 820400-901
PrepHT 2/ 17 x 7.5 5 820212-921 820212-918 820212-924 820212-924
820444-901 820444-901 820444-901 820444-901
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97

ZORBAX 300Å StableBond ()
(mm)
(µm)
300SB-C18
USP L1
300SB-C8
USP L7
0.5 x 250 5 5064-8266
0.5 x 150 5 5064-8264
0.5 x 35 5 5064-8294
* 0.5 x 150 3.5 5064-8268
* 0.5 x 35 3.5 5065-4459
0.3 x 250 5 5064-8265
0.3 x 150 5 5064-8263
0.3 x 35 5 5064-8295
* 0.3 x 150 3.5 5064-8267 5065-4460
* 0.3 x 100 3.5 5064-8259 5065-4461
* 0.3 x 35 3.5 5064-8270 5065-4462
* 0.3 x 50 3.5 5064-8300 5065-4463
(PEEK )
** 0.1 x 150 3.5 5065-9910
** 0.075 x 150 3.5 5065-9911
* 0.075 x 50 3.5 5065-9924 5065-9923
/5/ 0.3 x 5 5 5065-9913 5065-9914
/ 5065-9915 5065-9915
*RR 3.5 µm
300SB-CN
USP L10
300SB-C3
USP L56
98

ZORBAX
ZORBAX 300Å Extend-C18
pHpH 2-11.5
pH
pH
LC/MS
ZORBAX 300Extend C-18 HPLC pH 2-11.5
pH
pH
pH pH
LC/MS
O
O
Si
Si
C18
C18
* pH
ZORBAX 300Extend-C18 80Å 180 m 2 /g 60°C 2.0-11.5 4%
* pH 8 60°C pH 8-11.5 40°C
Silica Support
300Extend-C18 pH
Extend-C18
C18-C18
ZORBAX Extend-C18
773450-902
4.6 x 150 mm, 5 µm
20% 20 mM
NH4OHpH 10.5
80%
1.5 mL/min
24°C
40°C
k Values
4--1-
Column Volumes of Mobile Phase
10,000
www.agilent.com/chem/store
99

Extend-C18 StableBond SB-C18 pH
A
B
C
ZORBAX SB-C18
883975-902
4.6 x 150 mm, 5 µm
ZORBAX Extend-C18
773450-902
4.6 x 150 mm, 5 µm
ZORBAX Rx-C18
883967-902
4.6 x 150 mm, 5 µm
50% 50% + 1% TFA
60% 40%
1.5 mL/min
90°C
Fraction of Toluene k Remaining
A
B
C
Column Volumes Mobile Phase
300Extend-C18 pH pH pH 2 pH 11.5
300Extend-C18 pH
pH pH
Extend-C18 LC/MS
ZORBAX Extend-C18
773700-902
2.1 x 150 mm, 5 µm
A 0.1% TFA
B0.085% TFA80%
A10 mM NH 4 OH
B10 mM NH 4 OH80%
0.2 mL/min
15 min 15-50% B
35°C
MS ESI - Vf 70V
4.5 kV, N2- 35 psi, 12 L/min.325°C
2.5 µL 50 pmol
IIIIII
B.E Boyes Separation and Analysis of Peptides at High pH
Using RP-HPLC/ESI-MS, 4th WCBP, San Francisco, CA Jan 2000.
TIC (200-1500 m/z)
TIC (200-1500 m/z)
min
min
pH pH pH
pH
Extend-C18 pH
m/z
m/z
100
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ZORBAX
ZORBAX 300Å Extend-C18
(mm) (µm)
4.6 x 250 5 770995-902
4.6 x 150 5 773995-902
4.6 x 150 3.5 763973-902
4.6 x 100 3.5 761973-902
4.6 x 50 3.5 765973-902
* 2.1 x 150 3.5 763750-902
* 2.1 x 100 3.5 761775-902
* 2.1 x 50 3.5 765750-902
4/ 4.6 x 12.5 5 820950-932
4/ 2.1 x 12.5 5 821125-932
820888-901
*RR 3.5 µm
* 0.3 x 150 3.5 5065-4464
* 0.3 x 100 3.5 5065-4465
* 0.3 x 75 3.5 5065-4466
* 0.3 x 50 3.5 5065-4467
www.agilent.com/chem/store
101

(Poroshell)
Porous Shell
SOLID
CORE
5 µm
(Poroshell)
300Å
SB-C18SB-C8 SB-C3
pH
ZORBAX Poroshell
1,000 kDa
Poroshell300SB pH 300Extend-C18
pH
300SB-C18300SB-C8300SB-C3 300Extend-C18
ZORBAX Poroshell
Poroshell
HPLC 500-1,000 kDA
Poroshell StableBond
pH 2-10 Poroshell
300Extend-C18
LC/MS
HPLC
Poroshell
102

ZORBAX
Poroshell
ZORBAX Poroshell 300SB-C18
660750-902
2.1 x 75 mm, 5 µm
A 0.1% TFA
B 0.07% TFA
3.0 mL/min
1.0 min5-100% B
70°C260 bar
215 nm
1. II
2.
3.
4.
5.
6.
7.
8.
ZORBAX Poroshell300SB 90%
A
B
ZORBAX Poroshell 300SB-C18
660750-902
2.1 x 75 mm, 5 µm
ZORBAX 300SB-C18
883750-902
2.1 x 150 mm, 5 µm
A95% 5%
0.1% TFA
B5% 95%
0.07% TFA
1 mL/min
0.208 mL/min
0-100%B = 12 min
0-100%B = 120 min
70°C
20 µL (0.22 µg/1 µL)
BSA
15 70 pmol
ZORBAX Poroshell
1/10
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103

ZORBAX Poroshell LC/MS
ZORBAX Poroshell 300SB-C18
661750-902
1.0 x 75 mm, 5 µm
A + 0.1%
B+ 0.1%
600 µL/min
5.5 min20-100% B
80°C
MS LC/MSESI - 6000 V
12 L/min
350°C
45 psi
140 V
600-2500
0.15 amu
0.06 min
1 µL
ZORBAX Poroshell 2.1 mm1.0 mm 0.5 mm
LC/MS 1.0 mm 0.5 mm
Poroshell LC/MS Poroshell
0.5 5 pmole MS Poroshell
MS
ZORBAX Poroshell
Poroshell Poroshell Poroshell Poroshell
(mm) (µm) 300SB-C18 300SB-C8 300SB-C3 300Extend-C18
2.1 x 75 5 660750-902 660750-906 660750-909 670750-902
1.0 x 75 5 661750-902 661750-906 661750-909 671750-902
0.5 x 75 5 5065-4468
4/ 2.1 x 12.5 5 821075-920 821075-918 821075-924
820888-901 820888-901 820888-901
3/ 1.0 x 17 5 5185-5968 5185-5968 5185-5968 5185-5968
104
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ZORBAX
ZORBAX Eclipse (AAA)
24
OPA FMOC
Agilent 1100/1200
ZORBAX Eclipse AAA
7.5 cm 14
9 15 cm 24 18
Agilent 1100/1200 HPLC OPA FMOC
5-50 pmolDADFLD
ZORBAX Eclipse-AAA 24
ZORBAX Eclipse AAA
963400-902
4.6 x 150 mm, 3.5 µm
A40 mM Na 2
HPO 4
pH 7.8
B
45:45:10 v/v
2 mL/min
40°C
24
1. Asp
2. Glu
3. Asn
4. Ser
5. Gln
6. His
7. Gly
8. Thr
9. Cit
10. Arg
11. Ala
12. Tyr
13. Cys
14. Val
15. Met
16. Nva
17. Trp
18. Phe
19. Ile
20. Leu
21. Lys
22. Hyp
23. Sar
24. Pro
Time (min)
24 18 4.6 x 75 mm Eclipse AAA
9
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105

ZORBAX Eclipse (AAA)
(mm) (µm)
4.6 x 150 5 993400-902
FLD 4.6 x 150
3.5 963400-902
4.6 x 75 3.5 966400-902
3.0 x 150 3.5 961400-302
4/ 4.6 x 12.5 5 820950-931
820888-901
L- L-
L- L- L-
L- L- L-
L- L- L-
L- L- L-
L-
L-
, 10 x 1 ml *
1 nmol/µl 5061-3330
250 pmol/µl 5061-3331
100 pmol/µl 5061-3332
25 pmol/µl 5061-3333
10 pmol/µl 5061-3334
5062-2478
4 1 g
*5062-2478 1 g
OPA , (OPA) 3- 0.4 M 5061-3335
10 mg/ml, 6 x 1 ml
FMOC , 9-2.5 mg/ml
5061-3337
1 ml, 10
, 100 ml 5061-3339
DTDPA () , , 5 g 5062-2479
106
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ZORBAX
ZORBAX GF-250 GF-450
pH (pH 3-8)
ZORBAX GF-250 GF-450
GF-250 GF-450 4,000-
900,000GF-250/GF-450
>90% pH
pH 3 pH 8GF-250 GF-450
3 ml/min (

ZORBAX GF-250 SEC
ZORBAX GF-250
884973-901
9.4 x 250 mm, 4 µm
200 mM pH 7.0
2 mL/min
254 nm
BioRad
1. 670,000 Da
2. g- 158,000 Da
3. 44,000 Da
4. 17,000 Da
5. B-12 1,350 Da
ZORBAX GF-250
GF-450
Time (min)
ABSA BSA
2
2
B.
2
1. BSA
2. BSA
3.
1. IgG
2. IgG 1K (MOPC 31C)
3. TrisNa 2
HPO 4
NaCl
1
3
1
3
3
Inj. #1 Inj. #2
1
0 5 10
Time (min)
0 1 2 3 4 5 6 7 8 9 10
Retention Time (min)
ZORBAX GF-450
884973-902
9.4 x 250 mm, 6 µm
ZORBAX GF-450
884973-902
9.4 x 250 mm, 6 µm
PBS
pH 7.4
3 mL/min
220 nm
BSA BSA
200 mM
pH 7.0/0.1%
2 mL/min
225 nm
10 mg 50 mM
pH 7.0
108
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ZORBAX
9.4 x 250 mm ZORBAX GF-250
ZORBAX GF-250
884973-701
4.6 x 250 mm, 4 µm
130 mM NaCl/20 mM KCl/
50 mM Na 2
HPO 4
pH 7.0
1 mL/min
210 nm
1
2
3
4
5
6
7
1. IgM 900,000 Da
2. 670,000 Da
3. ß- 200,000 Da
4. 67,000 Da
5. 45,000 Da
6. 13,700 Da
7. 65 Da
4 6 8 10 12 14
Time (min)
ZORBAX GF-250
GF-250 K* K*
3.0
3.0
K*
2.5
2.0
1.5
1.0
0.5
BSA
Ovalbumin
Lysozyme
NaN 3
K*
2.5
2.0
1.5
1.0
0.5
BSA
Ovalbumin
Lysozyme
NaN 3
0.0
0 1000 2000 3000
Column Volumes
0.0
0 1000 2000 3000
Column Volumes
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109

ZORBAX GF-250 GF-450
(mm) (µm)
GF-250150Å 9.4 x 250 4 884973-901
GF-250150Å 4.6 x 250 4 884973-701
GF-450150Å 9.4 x 250 6 884973-902
()
GF-250 Diol2/ 9.4 x 15 6 820675-111
GF-250 Diol4/ 4.6 x 12.5 6 820950-911
GF-450 Diol2/ 9.4 x 15 6 820675-111
GF-250 Diol4/ 4.6 x 12.5 6 820950-911
9.4 x 15 840140-901
820888-901
PrepHT
PrepHT GF-250150Å 21.2 x 250 6 877974-901
PrepHT GF-450300Å 21.2 x 250 6 877974-910
PrepHT 2/ 820400-901
PrepHT GF-2502/ 17 x 7.5 6 820212-911
PrepHT GF-4502/ 17 x 7.5 6 820212-911
820444-901
110

ZORBAX
ZORBAX SAX SCX
ZORBAX SAX 300SCX Zorbax
pH 2-7
ZORBAX Bio-SCX II
Zorbax (SAX) (300SCX)
5 µm
Zorbax Bio-SCX II LC/MS
3.5 µm Zorbax
pH
ZORBAX SAX 70Å 300 m 2 /g 2.0-7.0 350 bar
ZORBAX 300SCX 300Å 50 m 2 /g 2.0-7.0 350 bar
ZORBAX Bio-SCX II 300Å 90 m 2 /g 2.5-8.5 350 bar
ZORBAX SAX SCX
(mm) (µm) SAX 300SCX
9.4 x 250 5 880952-203 880952-204
4.6 x 250 5 880952-703 880952-704
4.6 x 150 5 883952-703 883952-704
4.6 x 50 5 846952-704
3.0 x 50 5 860700-304
2.1 x 150 5 883700-704
2.1 x 50 5 860700-704
Bio-SCX
II
0.3 x 35 3.5 5065-9912
0.8 x 50 3.5 5065-9942
4/ 4.6 x 12.5 6 820950-903 820950-904
820888-901 820888-901
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111

HPLC
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HPLC
HPLC
800-820-3278 www.agilent.com/chem/techsupport
112

ZORBAX
Eclipse Plus
•
•
•
Eclipse Plus
Eclipse Plus
•
•
• pH
Eclipse Plus
1. 2. 60/40/
Eclipse Plus C18
Ace 5 C18
SunFire C18
XBridge C18
Discovery HS C18
Luna C18 (2)
Gemini C18
XTerra MS C18

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