saqarTvelos samedicino Jurnali Medical Journal of Georgia

21firinebis cvlis Taviseburebebis SeswavlismizniT, eqsperimentis dawyebidan,yoveli aTi dRis Semdeg, viRebdiT sisxlisI, II, III jgufis da intaqturi cxovelebiskudis venidan (TiToeuli jgufidan 17cxoveli) da porfirinebis fluoroscentulinaTebis xarisxs eriTrocitebSivafasebdiT maTi metabolizmis intensivobisSefasebis mizniT.lipiduri fraqciebis raodenobrivigansazRvrisaTvis masalas (sisxlis plazma,eriTrocitebi, gulis kunTi, filtvebi)viRebdiT kriostabilur pirobebSi davamuSavebdiT Bloor-is meTodiT, Одушко-sada Зеленина-s modifikaciiT. damuSavebulimasalis analizi Txel fenebSi xorcieldebaqromatografiis meTodiT mzafirfitebze Silufo UV-254. lipidurifraqciebis raodenobriv analizs vawarmoebdiTKarl Zeiss-is firmis eqsktinkciuliregistratoriT ERJ-10-iT. sisxlSi(plazma da eriTrocitebi), gulis kunTsada filtvebSi, lipidebis fraqciis raodenobivmaCveneblebs vsazRvravdiT mg%-Si.miRebuli masalis statistikuri damuSavebisaTvissarwmuno sxvaoba (P) ganisazRvrebodafiSer-stiudensis cxrilismeSveobiT realuri sxvaobis (t) koeficientisgaTvaliswinebiT.histologiuri kvlevisaTvis masalis(gulis kunTi, filtvebi) fiqsacia warmoebda12%-iani formaliniT, gauwylovanebaxdeboda aRmaval spirtebSi, xolovayalibebdiT celoidinSi (Celloidin inFlocken, VEB Laborachemi, DDR). marxilianmikrotomze daWrili anaTlebi iRebebodahemotoqsiliniTa da eoziniT.eriTrocitebi, gulis kunTsa dafiltvebSi ultrastruqturuli cvlilebebisgamosavlenad viyenebdiTeleqtronuli mikroskopiis meTods.masalas fiqsacias vawarmoebiT s-kolidinisbuferze momzadebuli glutaraldehidisWeibel-is fiqsatoriT da amavebuferze momzadebuli osmiumis fiqsatoriT- Bennett-Luft-is mixedviT. masalasvayalibebdiT epon-aralditis narevSiMollenhauer-is mixedviT. ultraTxelianaTlebis miReba xdeboda ultratom LKBIII-sa da Reichert Ultracut-E-ze. masalis ultrastruqturulianalizisaTvis viyenebdiTeleqtronul mikroskopebs Tesla BS-500-sa daЭМВ-100A-s.eqsperimentma gamoavlina mniSvnelovaniZvrebi sisxlis, gulis kunTisa dafiltvebis bioqimiur da struqturulorganizaciaSi. kerZod, tyviis ionebiseqspozicia periferiul sisxlSi iwvevsretikulocitebisa da eriTrocitebisbazofiluri marcvlovanebiT raodenobismomatebas. sakuTari kvlevis Sedegebi,naTlad miuTiTebs saeqsperimento cxovelebistyviis maRali doziTdatvirTvis pirobebSi eriTrocitebSiporfirinebis sinTezis Semcirebaze, ma-Sin, rodesac dabali doza aaqtivebs porfirinebisanabolizms. zemoTqmuli da hemoglobinisraodenobis Semcireba organizmSiganapirobebs anemiis fonis Camoyalibebasda eriTropoezis intensifikacias.amasTan, atipiuri hemoglobinis sin-Tezis, sisxlis plazmaSi uxeSdispersiulicilebis fraqciis momatebis, sisxlisdinebis siswrafis Semcirebisa da sisxlissiblantis momatebis pirobebSi aRiniSnebawvrili kalibris sisxlZarRvebSi tyviiTdatvirTuli gazrdili kuTri wonisada Secvlili muxtis mqone eriTrocitebisagregacia.tyvia iwvevs lipoproteinuli membranebislipiduri fraqciis fosfolipidebis,qolesterinisa da qolesteriniseTerebis metabolizmis Secvlas, ris SedegadacirRveva lipoproteinuli membranebis,rogorc funqciuri, aseve, bunebrivia,struqturuli organizaciac. membranebisfunqciis Secvlis Sedegad tyviisionebi advilad aRwevs yvela ujredSidakomponentTan, aqtiurad monawileobsda araspecifikurad warmarTavs mis metabolizms.eriTrocitebis ultrastruqturisgamokvleviT aRmoCnda, rom, hemoglobin-Semcveli marcvlebis raodenobis Semcirebisgamo, isini sxvadasxva simkvrivisaa.didi raodenobiT aRiniSneba, agreTve,eriTrocitTa Crdilebi, rac maT liziszemiuTiTebs. ujredebSi aRiniSneba heincerlihissxeulakebi, romlebic normuleriTrocitebSi ar vlindeba. igi rogorcwesi, warmoiqmneba im organizmTaeriTrocitebSi, romlebsac gaaCnia arastabilurihemoglobini. eriTrocitebisSemomsazRvreli membranebi gauxeSebulia,dawyvetilia da maTSi warmoiqmnebaporebi. membranebi alag ganicdis sruldezorganizacias. imis gaTvaliswinebiT,rom eriTrocitebis membranas gaaCniamniSvnelovani funqcia, savaraudoa, romeriTrocitebis membranebis struqturulimodifikacia gamoiwvevs maTi da, Sesabamisad,sisxlis funqciuri aqtivobisSecvlas.gulis kunTi ganicdis energiis deficitisada masTan dakavSirebuli meqanikurifunqciis darRvevisaTvis damaxasiaTebel