PeloNews Spring 2024

PELONews

PELONews Spring 2024 Updates and Points-of-View Upcoming conferences to meet PELOBiotech in person The ELRIG Forum on March 7th, 2024 at the Science and Congress center "darmstadtium" in Darmstadt. We will be at booth number 5! Make sure to stop by our booths at all of these events! The IGLD symposium from the 7 - 9th of March 2024, at the Congress Hall at the Zoo, in Leipzig. You can find us at lucky booth number 13! The 55th Annual Conference of the German Society for Mass Spectrometry (DGMS) at Technical University of Munich, in Freising from 10 - 13th of March 2024. Looking a few months forward, our local sales manager, Cassandra Deichsel will be at the 3R Länd Conference on May 21-23rd, 2024 in Tübingen. Enhancing MSC-EVs Production: The Benefits of Defined Media • Ethics: The use of fetal bovine serum (FBS) in cell culture media raises ethical concerns due to its origin. In contrast, defined media for MSC-EV production eliminates the need for FBS, aligning with ethical considerations by reducing reliance on animal-derived components. • Consistency: Defined media play a crucial role in ensuring consistent and reproducible manufacturing processes for MSC-EVs. The precisely formulated composition of defined media enhances the reliability of MSC-EV production, leading to consistent results across experiments. • Control: The precise formulation of defined media provides researchers with greater control over MSC-EV production. This control allows for optimized scalability and efficiency in manufacturing processes, contributing to improved standardization and quality. • Optimization: Tailoring the composition of defined media for MSC-EV production can significantly impact the yield, purity, and functionality of EVs. By optimizing the media components, researchers can enhance the overall quality and therapeutic potential of MSC-EVs. • Safety: Defined media minimize the risk of contaminants or unwanted effects in production, ensuring high-quality vesicles. By eliminating potential adverse immune reactions from undefined components, defined media enhance the safety and efficacy of these therapies. • Compliance: The use of GMP quality defined media for MSC-EV production aligns with regulatory guidelines and ethical considerations, meeting the necessary standards for EV-based research and therapeutic applications. By adhering to these guidelines, researchers can ensure compliance with regulatory requirements and ethical principles in MSC-EV studies. 4 www.pelobiotech.com

Overview Table 1. Comparison of Cell Culture Factors for Cell Growth and Exosome Studies. Adapted from Liau et al, Int J Mol Sci, 2021 Factors FBS hPL Defined Media EXO Free Media Pros Abundant and accessible Easy to produce Well-defined components Exosome-depleted and ideal for isolation Suitable for most cells Suitable for most cells No batch-to-batch variation No batch-to-batch variation Supports cell survival and proliferation Supports cell survival and proliferation No risk of xenogeneic/ allogeneic protein contamination No risk of xenogeneic/allogeneic protein contamination Widely used in studies and publications No risk of xenogenic immune reactions; can be produced using autologous blood No unintended interaction with test substances No unintended interaction with test substances Cons Components are illdefined Components are illdefined Not available for certain cell types Not available for certain cells Batch-to-batch variation Batch-to-batch variation (reduced by pooling) Some media require extra coating for cell attachment Some media require extra coating for cell attachment Potential contamination (prions, mycoplasma, bacteria, fungi) Unintended interaction with test substances More expensive compared to alternatives More expensive compared to alternatives Risk of animal protein contamination in clinical usage Few distributors Time-consuming and difficult to develop Time-consuming and difficult to develop Unintended interaction with test substances Potential allogenic immune response Higher particle count and presence of exosomes Ethical concerns with animal welfare Risk of transmitting human viruses Multitude of exosomes not suitable for isolation or studies Multitude of exosomes not suitable for isolation or studies www.pelobiotech.com 5