PeloNews Spring 2024

PELONEWS
WITH A FOCUS ON MSC-EV RESEARCH
PELOBiotech Newsletter Spring 2024
"The science of today
is the technology of
tomorrow."
– Edward Teller,
Hungarian-American
theoretical physicist
Dear Scientist,
In the realm of regenerative medicine, Mesenchymal Stem / Stromal cell
derived Extracellular Vesicles (MSC-EVs) have emerged as a focal point of
research and therapeutic exploration. These nanoscale membranous
structures, released by Mesenchymal Stem/ Stromal cells, encapsulate a
diverse array of bioactive molecules, including proteins, lipids, and nucleic
acids, reflecting the physiological state of their parent cells and exhibiting
the capacity to modulate the behavior of recipient cells through intricate
signalling mechanisms.
The therapeutic potential of MSC-EVs is underscored by their ability to
mimic the reparative and regenerative properties of MSCs, offering a
promising avenue for targeted drug delivery systems and tissue repair
strategies. Ongoing studies have delved into understanding the
mechanisms of MSC-EV interactions with target cells, optimizing isolation
and purification techniques, and evaluating their efficacy and safety
profiles in preclinical and clinical settings.
Join us in exploring the fascinating world of MSC-EVs and their
transformative potential in regenerative medicine. As we delve deeper into
the complexities of MSC-EVs, addressing some key aspects and critical
role of defined media in enhancing EV quality.
All the best to you and your research,
Christiane Büchsel
Head of Marketing and Communications at PELOBiotech
CONTENT
Overview of MSC-EVs
Sneak peek of the first
annual PELOBiotech
Conference Advancement in
Non-Animal Research and
Translational Medicine"
Why is defined
media good for MSCs?
Media and
supplements for MSCs
GMP-quality MSCs
Upcoming PELOAcademy
Webinars

PELONews Spring 2024
Overview MSC-EVs
Reprinted from “Extracellular Vesicle Separation by Density Gradient Ultracentrifugation”, by BioRender.com
The culture and expansion of
Mesenchymal Stem/ Stromal Cells
(MSCs) play a crucial role in exosome
production, with the choice of expansion
method significantly affecting the yield
and quality of exosomes. Researchers
have been investing immortalized MSC
(iMSC) lines, created by inducing the
expression of human telomerase reverse
transcriptase (hTERT), as a potential
solution to the senescence issue
encountered during primary MSC culture
expansion. While iMSC lines offer a
reliable and scalable source for exosome
production, concerns exist regarding
their functional alterations compared to
primary MSCs.
Also, the selection of culture media and serum for MSCs culture and expansion can also affect the
exosome quality and yield. The choice of isolation technique is crucial as it impacts exosome purity
and yield for therapeutic applications. Techniques like ultrafiltration, density gradient purification,
size-based isolation, polymer precipitation, and immuno-affinity purification have been
developed for exosome purification, with tangential flow filtration being recommended for
industrial-scale manufacturing due to its efficiency in concentrating extracellular vesicles from large
fluid volumes, providing with diverse options for efficient exosome extraction in various biomedical
applications.
PELONews: MSCs and Exosome Production:
A Gateway to Regenerative Medicine.
Scan/Click the QR code to read our article to learn more on the topic.
PELOAcademy | Clinical Potential of MSC-EVs and Translational Challenges
In our most recent webinar by Prof. Dr. rer. nat. Bernd Giebel of
the University Hospital of Essen on the Clinical Potential of MSC-
EVs and Translational Challenges, the heterogenous nature of
both MSCs and EVs was discussed as well as the effectiveness of
EV-therapy. Go to our website to watch the webinar on-demand:
https://www.pelobiotech.com/webinars.html
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Announcement
PELONews: Our first annual conference will be in June!
At our conference, we aim to spark engaging discussions and foster collaborations within the field.
Join us as we gather to explore innovative approaches, share first-hand insights, and instigate
meaningful conversations all surrounding the topic of Advancements in Animal-Free &Translational
Research. Get more information & sign up by email info@pelobiotech.com.
www.pelobiotech.com 3

PELONews Spring 2024
Updates and Points-of-View
Upcoming conferences to meet PELOBiotech in person
The ELRIG Forum on March 7th, 2024 at the Science and Congress center
"darmstadtium" in Darmstadt. We will be at booth number 5!
Make sure to stop by our booths at all of these events!
The IGLD symposium from the 7 - 9th of March 2024, at
the Congress Hall at the Zoo, in Leipzig. You can find us at
lucky booth number 13!
The 55th Annual Conference of the German Society for Mass Spectrometry
(DGMS) at Technical University of Munich, in Freising from
10 - 13th of March 2024.
Looking a few months forward, our local sales manager,
Cassandra Deichsel will be at the 3R Länd Conference on May
21-23rd, 2024 in Tübingen.
Enhancing MSC-EVs Production: The Benefits of Defined Media
• Ethics: The use of fetal bovine serum (FBS) in cell culture media raises ethical concerns due to
its origin. In contrast, defined media for MSC-EV production eliminates the need for FBS,
aligning with ethical considerations by reducing reliance on animal-derived components.
• Consistency: Defined media play a crucial role in ensuring consistent and reproducible
manufacturing processes for MSC-EVs. The precisely formulated composition of defined media
enhances the reliability of MSC-EV production, leading to consistent results across experiments.
• Control: The precise formulation of defined media provides researchers with greater control
over MSC-EV production. This control allows for optimized scalability and efficiency in
manufacturing processes, contributing to improved standardization and quality.
• Optimization: Tailoring the composition of defined media for MSC-EV production can
significantly impact the yield, purity, and functionality of EVs. By optimizing the media
components, researchers can enhance the overall quality and therapeutic potential of MSC-EVs.
• Safety: Defined media minimize the risk of contaminants or unwanted effects in production,
ensuring high-quality vesicles. By eliminating potential adverse immune reactions from
undefined components, defined media enhance the safety and efficacy of these therapies.
• Compliance: The use of GMP quality defined media for MSC-EV production aligns with
regulatory guidelines and ethical considerations, meeting the necessary standards for EV-based
research and therapeutic applications. By adhering to these guidelines, researchers can ensure
compliance with regulatory requirements and ethical principles in MSC-EV studies.
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Overview
Table 1. Comparison of Cell Culture Factors for Cell Growth and Exosome Studies. Adapted from
Liau et al, Int J Mol Sci, 2021
Factors FBS hPL Defined Media EXO Free Media
Pros
Abundant and
accessible
Easy to produce
Well-defined
components
Exosome-depleted and ideal for
isolation
Suitable for most cells Suitable for most cells No batch-to-batch
variation
No batch-to-batch variation
Supports cell survival
and proliferation
Supports cell survival
and proliferation
No risk of xenogeneic/
allogeneic protein
contamination
No risk of xenogeneic/allogeneic
protein contamination
Widely used in studies
and publications
No risk of xenogenic
immune reactions; can
be produced using
autologous blood
No unintended
interaction with test
substances
No unintended interaction with test
substances
Cons
Components are illdefined
Components are illdefined
Not available for
certain cell types
Not available for certain cells
Batch-to-batch
variation
Batch-to-batch
variation (reduced by
pooling)
Some media require
extra coating for cell
attachment
Some media require extra coating
for cell attachment
Potential
contamination (prions,
mycoplasma, bacteria,
fungi)
Unintended interaction
with test substances
More expensive
compared to
alternatives
More expensive compared to
alternatives
Risk of animal protein
contamination in
clinical usage
Few distributors
Time-consuming and
difficult to develop
Time-consuming and difficult to
develop
Unintended interaction
with test substances
Potential allogenic
immune response
Higher particle count
and presence of
exosomes
Ethical concerns with
animal welfare
Risk of transmitting
human viruses
Multitude of exosomes
not suitable for
isolation or studies
Multitude of exosomes
not suitable for
isolation or studies
www.pelobiotech.com 5

PELONews Spring 2024
Products
Specialized Media and Supplements for MSCs
To optimize EV isolation from MSCs, we advise using tailored products for each of the distinct
stages, including isolation, maintenance, proliferation, and EV isolation.
Made with BioRender 2023
For those seeking customization and control over their cell culture conditions, but wanting to use
your own basal medium, we recommend using Human Platelet Lysate (hPL) serum supplementation
from our trusted partner AventaCell. This can come in exosome-depleted versions which is perfect
for implementing more control over your EVs being produced.
Exosome
depleted
UltraGRO
PURE GI
Xeno-free, exosome-depleted, gamma-irradiated, and fibrinogen-depleted hPL
available for research, clinical, and commercial use. At the moment, GMP qualities
of gamma-irradiated hPL are available, while we wait for the GMP to come in the
exosome-depleted version.
Cat #: PB-HPCHEFRLI- - Quantities available: 50, 100, and 500 ml
Alternatively, for those looking for complete off-the-shelf solutions of the highest quality standards,
but still in research quality, PELOBiotech offers the following two media options in our Cellovations ©
brand!
MSC growth
medium
(ready-touse)
This medium consists of specially formulated MSC basal medium with added 10%
fetal bovine serum and a mixture of growth factors to promote MSC
proliferation. The medium is pre-mixed, ready to go, so it can be used
immediately upon arrival!
Cat #: PB-MH-675-0000-rtu
500ml Bottle
MSC media
kit, enhanced
xeno-free
Specialized defined media without any FBS. The media consists of optimized MSC
basal medium and a cell culture supplement which contains a human platelet
lysate derived growth factor mixture. When you are ready to use the medium,
thaw and add the supplements to the basal medium, and you are ready to go.
Cat #: PB-C-MH-675-0511-XF
Yields 500ml of Medium
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Products
Looking for complete off-the-shelf solutions but with clinical applications in mind ? We recommend
chemically defined media in GMP quality from our partner Xcell.
CellCor
MSC CD
AOF
Animal origin-free chemically defined media for human-derived mesenchymal
stem cells growth and proliferation composed of synthetic and recombinant
proteins, rigorously tested for bioactivity, endotoxins, mycoplasma, and more.
Cat #: PB-YSP018
500 ml bottle
CellCor
EXO CD
Chemically defined media for stable MSC growth from different tissues, enabling
high-quality exosome production. Versatile and compatible with various methods
for consistent, reproducible results. Ideal for research and clinical applications.
Promotes low aging and high genetic stability in MSCs from Adipose, Bone
marrow, Cord blood, and Wharton jelly.
Cat #: PB-YSP017
500ml bottle
GMP-quality MSCs
Now moving to the cells themselves, are you isolating and producing your own MSCs
but are interested in potentially purchasing the cells in GMP quality? Did you know
we carry bone-marrow derived MSCs in GMP quality from the renowned Swedish
company Cellcolabs.
These MSCs are:
• Produced in a facility that is EU-GMP for iATMP approved.
• Sourced from the bone marrow of young, healthy donors
• Entirely xeno-free
• Fully characterized according to the ISCT criteria
Cellcolabs also produces EVs produced from the GMP quality human bone-marrow derived MSCs!
The EVs are purified with a 0.22uM filter, characterized through the expression of CD9, CD63, and
CD81 and lastly their size and number is verified using Zetaview.
Want to try out these EVs for your application? Let’s have a conversation and make this happen!
PELONews: Stay tuned for new xeno-free hydrogels!
www.pelobiotech.com 7

PELONews Spring 2024
PELONews Spring/Summer 2023
PELOAcademy Webinars
If you have any
queries or if you like
what you see, reach
out!
+49 89 517 286 59 0
info@pelobiotech.com
www.pelobiotech.com
Have you attended a PELOAcademy webinar yet? Take a look at our
webinar line-up for the first half of 2024. All are available on-demand.
Next up we will be exploring the advantages of Animal-Component free
media for cell culture on March 20 at 10 AM with Dr. Lothar Steeb, CSO
& CEO PELOBiotech Don’t forget to mark it in your calendars!
Sign up at pelobiotech.com/webinars.html
PELOInternal
As we began the new year,
the PELOBiotech family
came together in a
heartwarming gathering to
mark multiple joyous
occasions. This gathering
was especially memorable
as we welcomed two
members into our company,
expanding our vibrant team
with fresh perspectives and
enthusiasm.
Simultaneously, we bid a
fond farewell to our
youngest team member,
Kevin, who is embarking on
an exciting journey to further his education abroad at Stanford
University. His dedication and contributions have left a mark on us, but
we are thrilled to watch him go after his goals. It was a very warm and
joyful occasion filled with good food, laughter, and drinks. Here's to a
year filled with growth, success, and boundless opportunities. May we
continue to thrive together as a cohesive team, facing new challenges
with courage and optimism.
PELOBiotech GmbH
Klopferspitz 19
82152 Planegg
Germany
Managing Directors:
Dr. Peter Frost and
Dr. Lothar Steeb
You like what you
see?
Sign up for PELONews
and the PELOClub on
our website!
New Faces at
PELOBiotech!
Julian Olmos
Sales Manager
Ovi Dixit
We are always happy to provide you with additional
information and product details. Simply fill in the product
information form on our website or email us!
Working Student
8 www.pelobiotech.com