PELONEWS Spring/Summer 2023

PELONews

PELONews Spring/Summer 2023 Organ/Cell based products Hepatocytes Primary human hepatocytes are the main cells in the liver, characterized by their cubical shape and abundant smooth endoplasmic reticulum. They exhibit eosinophilic cytoplasm, basophilic stippling, and round nuclei with dispersed chromatin. Hepatocytes form plates separated by sinusoids, which have fenestrated endothelial linings and are supported by reticulin. Kupffer cells and stellate cells are present in the sinusoids, performing phagocytosis and storing vitamin A, respectively. These hepatocytes “gold standard” for in vitro hepatic assays such as Drug Metabolism and Pharmacokinetics (DMPK). When choosing our primary human hepatocytes, you gain access to extensive donor information and large lot sizes of up to 1000 vials. The hepatocytes are evaluated for critical inductions such as CYP1A2, CYP2B6, and CYP3A4, and their metabolic activity is assessed for various essential enzymes which includes CYP450 enzymes (1A2, 2B6, 2C8, 2C9, 2C19, 2D6, 3A), ECOD, SULT, UGT, and AO. The cells are available in plateable, suspension, and spheroid-qualified forms. By selecting these hepatocytes, you can explore the intricacies of liver function with confidence. With exceptional post-thaw viability (>85%) this allows for the study of xenobiotic metabolism, liver toxicity, cell biology, and host-pathogen interactions in a cost-effective manner. Read more about the use of hepatocytes in research by scanning/clicking the QR code: If you would like to purchase hepatocytes please contact us for more information. High-quality tissues by Anabios Precision-cut lung slices with exceptional RNA integrity (RIN 9.5) from normal and diseased tissue samples. These slices retain their functionality in ex-vivo assays, closely resembling fresh precisioncut lung slices. Additionally, we have included detailed donor information for enhanced research relevance. Other products include: Isolated cardiomyocytes (To predict druginduced proarrhythmia and inotropic risk), adult human dorsal root ganglia tissue and adult human CNS tissue 10 I www.pelobiotech.com

Overview Organoids & Organ-on-a-chip PELONews: 199 People have opted to be cryopreserved, we do not know if revival is possible for them right now but our ThawSTAR CFT2 ® revives cells automatically with high efficiently and reproducible thawing. Enzymes for cell isolation & purification Different types of enzymes, such as collagenase, trypsin, dispase, and DNAse, are used in cell isolation processes. The choice of enzyme depends on the specific cell type and tissue being targeted. High-quality enzymes are essential for achieving high yield and functional cells during isolation. Quality enzymes ensure efficient and complete tissue digestion, leading to optimal cell dissociation. Enzymes with high specific activity and purity minimize non-specific cell damage and maximize cell viability. Contaminants or impurities in low-quality enzymes can negatively affect cell yield and compromise cell functionality. Well-characterized and standardized enzymatic activity ensures consistent and reproducible results. 8. Consistent activity levels of enzyme batches allow for reliable and predictable cell isolation outcomes. To achieve exceptional and consistent outcomes, we highly recommend our partner, VitaCytes enzymes, for the isolation of cardiomyocytes, skin cells, liver cells, islet cells, adipose cells, and stem cells from humans, pigs, and rodents. Additionally, VitaCytes offers personalized enzyme solutions tailored to meet your specific requirements. 11