PELONEWS Spring/Summer 2023

PELONEWS 

WITH FOCUS ON EXTRAVESICULAR RESEARCH 

PELOBiotech Newsletter Spring/Summer 2023 

Everything is 

theoretically 

impossible, 

until it is done. 

– Robert A. Heinlein, 

American Science 

fiction writer 

Dear Scientist, 

Extracellular vesicles (EV) are the lipid-bound nano particles 

which are secreted by cells which act as key mediators of many 

physiological processes (L.M Doyle et al. 2019). EVs are the 

mediators of cell to cell communication by transporting different 

lipids, proteins and nucleic acids to recepient cells. EVs secreted 

by cancer cells promote cell growth and survival which may help 

in progression of invasive and metastatic activity.(W. H Chang et 

al., 2021). There has been a lot of research which shows 

prospects of EVs as modern drug delivery vehicles and as next 

generation therapeutics, though there still is a lot of 

criticism (I. K Hermann et al., 2021). 

You can check the solutions we have for your project 

on page 4. 

All the best to you and your research, 

Christiane Büchsel 

———————————————————————— 

PELOAcademy | Biomimicry at Nanoscale Level: 

a Step forward Towards Organotypic Cell Studies 

CONTENT 

• Extracellular Vesicles 

• Organoids and organ-on-achip 

• 3-in-1 Plate a new 3D Cell 

Culture Technology 

• Extracellular Matrices: Extra- 

Gel an equivalent to Matrigel 

and Defined Solutions 

• Liver Cells/ Hepatocytes 

• New suppliers and products 

presented by Prof. Matteo Santin, 

University of Brighton, UK 

Date and Time: Tue, June 20; 10 AM 

Save your spot now at 

https://www.pelobiotech.com/webinars.html

PELONews Spring/Summer 2023 

Overview Extracellular Vesicles 

Exosomes are small, membranebound 

extracellular vesicles (EVs) that 

are released by cells, such as 

adipose-derived stem cells/ 

stroma cells, mesenchymal 

stem cells /stroma cells(MSC), 

dendritic cells, T and B cells. 

These vesicles can contain a wide 

variety of molecules, such as 

proteins, lipids, and nucleic acids, 

that are derived from the cell of 

origin. 

They play a key role in intercellular 

communication, as they can transfer 

contents to other cells and influence 

their behavior. They have been 

implicated in a wide variety of 

physiological and pathological 

processes, including immune 

regulation, tissue repair, and cancer 

progression. They are also used as a 

tool for drug delivery and as a 

biomarker for disease diagnosis and 

prognosis. 

Scan/click the QR code to learn more about exosomes! 

Why is defined media so excellent? 

• Ethics: FBS is derived from the blood of unborn calves, raising ethical concerns regarding animal 

welfare. In contrast, defined media eliminate the need for FBS, reducing reliance on animalderived 

components. . 

• Consistency: Defined media ensure consistent and reproducible manufacturing processes as it is 

precisely formulated. 

• Control: Precisely formulated composition provides control over exosome production and 

thereby improves scalability and efficiency in manufacturing 

• Optimization: Tailoring media composition enhances exosome yield, purity, and functionality. 

• Standardization: Eliminating undefined components like serum ensures standardized exosome 

preparation and uniform reproducible results. 

• Safety: Minimizing contaminants and unwanted effects with defined media ensures high-quality 

exosomes such as adverse immune reactions associated with undefined components.. 

• Compliance: Meeting regulatory guidelines and ethical considerations in exosome production. 

Defined media reduce the risk of potential contaminants or unwanted biological effects By precisely 

defining the media composition, unwanted variations and impurities can be minimized, ensuring 

the production of high-quality exosomes. 

2 I www.pelobiotech.com


Options: 

A) Belgian beer 

B) Defined Media 

C) Pooled urine 

D) Other 

Go to Page 7 for the 

answer. 

PELONews: What Makes Exosomes and 

Stem Cell Therapy the Cell-ebrities of 

Regenerative Medicine? 

Scan/Click the QR code to read our article to find out. 

Media for Exosomes 

Gain more Control with Defined Media for EXOsome isolation 

CellCor EXO CD 

is a chemically defined media that supports the stable growth and 

characteristics of MSCs from different tissues. It enables the 

production of high-quality exosomes, essential for various 

applications, including regenerative medicine and drug delivery. The 

media is versatile and compatible with different culture methods and 

systems, allowing for consistent and reproducible results. Its stability 

and compatibility make it an excellent option 

for research and clinical applications. It 

promotes low aging and high genetic 

stability of cells, making it appropriate for 

MSCs from various tissues, including 

Adipose, Bone marrow, Cord blood, and 

Wharton jelly. 

3 

New 

Products !



Table 1. Comparison of Cell Culture Factors for Cell Growth and Exosome Studies. Adapted from 

Liau et al, Int J Mol Sci, 2021 

Factors FBS hPL Defined Media EXO Free Media 

Pros 

Abundant and 

accessible 

Easy to produce 

Well-defined 

components 

Exosome-depleted and ideal for 

isolation 

Suitable for most cells Suitable for most cells No batch-to-batch 

variation 

No batch-to-batch variation 

Supports cell survival 

and proliferation 

Supports cell survival 

and proliferation 

No risk of xenogeneic/ 

allogeneic protein 

contamination 

No risk of xenogeneic/allogeneic 

protein contamination 

Widely used in studies 

and publications 

No risk of xenogenic 

immune reaction; can 

be produced using 

autologous blood 

No unintended 

interaction with test 

substances 

No unintended interaction with test 

substances 

Cons 

Components are illdefined 

Components are illdefined 

Not available for 

certain cells 

Not available for certain cells 

Batch-to-batch 

variation 

Batch-to-batch 

variation (reduced by 

pooling) 

Some media require 

extra coating for cell 

attachment 

Some media require extra coating 

for cell attachment 

Potential 

contamination (prions, 

mycoplasma, bacteria, 

fungi) 

Unintended interaction 

with test substances 

More expensive 

compared to 

alternatives 

More expensive compared to 

alternatives 

Risk of animal protein 

contamination in 

clinical usage 

Few distributors 

Time-consuming and 

difficult to develop 

Time-consuming and difficult to 

develop 

Unintended interaction 

with test substances 

Potential allogenic 

immune response 

Higher particle count 

and presence of 

exosomes 

Ethical concerns with 

animal welfare 

Risk of transmitting 

human viruses 

Multitude of exosomes 

not suitable for 

isolation or studies 

Multitude of exosomes 

not suitable for 

isolation or studies 



Media for cell and gene therapy applications 

Media for Stem cell maintenance and proliferation 

CellCor CD MSC 

Choosing the right media is crucial for achieving optimal growth of 

human MSCs with the desired characteristics. CellCor CD MSC is 

an excellent choice for controlling culture conditions, as it is made up 

of chemically defined components. This product can maintain stable 

stemness, differentiation, and marker expression throughout subculturing, 

up to the second half. Furthermore, it supports low aging 

and high genetic stability of cells and is suitable for MSCs from 

different tissues such as Adipose, Bone marrow, Cord blood, and 

Wharton jelly. It can also be used for the 

production and research of stem cell-derived 

exosomes. 

Scan the QR-Code and get the 

Product sheet. 

5 

New 

Products !

PELONews Spring/Summer2023 

Overview Organoids & Organ-on-a-chip 

Organoids, and organ-on-a-chip, have 

transformed the landscape of biomedical research 

and clinical applications. 

Organ-on-a-chip platforms replicate the complex 

microenvironment of organs, integrating multiple 

cell types and physiological functions onto a single 

chip. 

These systems enable researchers to study organlevel 

responses, drug interactions, and disease 

mechanisms with high precision. 

The combination of organoids and organ-on-achip 

technology offers a powerful approach to 

simulate human organ systems and bridge the 

gap between traditional in vitro models and 

animal testing. 

This advancement not only enhances our understanding of human biology and disease but also 

contributes to the development of safer and more effective treatments, while reducing reliance on 

animal models. The FDA's recognition of these technologies by no longer requiring animal testing 

reinforces the importance of embracing human-relevant models and ethical alternatives 

for advancing healthcare and scientific discoveries. 

Read our article by scanning/clicking the QR code to find out more. 

Intestine-on-a-Chip for Personalized Medicine 

Intestine-on-a-chip models also 

facilitate the study of gastrointestinal 

diseases, e.g. inflammatory bowel 

disease, by mimicking the 

inflammatory responses and barrier 

dysfunction observed in these 

conditions. 

Organoid products for R&D or GMP 

• Organoid kits with cytokines, media and cells included for culture of organoids 

• PhenoDrive 

• ECM Mixtures 

• Scilia ExtraGel ECM equivalent to the gold-standard in the industry. 

• Apricell 3-in-1 plate for high throughput drug testing. 

• Collagen (I, II, III, IV) 

• Fibronectin 

• Laminin 

• Vitronectin 

Scan/click the QR code to learn about out ECM for 2D & 3D cell culture. 



PELONews: Can lab-grown brains become 

conscious? What is consciousness? Are we 

nothing more than an intricate neuronal 

circuit? 

Scan/Click the QR code to read our article here to find out. 

Apricell 3-in-1 plate 

To study co-culture 

Tumour invasion 

ECM study 

Drug screening 

Scilia ExtraGel ECM 

Reconstituted matrix hydrogel 

3D cell culture 

Angiogenesis 

Stem cell maintenance and 

differentiation 

In-vivo xenograft generation 

Organoid kits 

With cell culture medium and 

cytokines to create cardiac, 

biliary, breast, brain, lung, 

liver, vascular organoids 

New 

Products ! 

7


New Products and Partners 

3D CoSeedis TM 

3D CoSeedis TM is a novel scaffold-free 

3D cell co-culture system. 

Some of the key features of 3D 

CoSeedis TM Chip: 

• They are highly reproducible 

• Easy for mass production of highly 

uniform organoids 

• Wide range of disease models 

• HTS compatible 

• Could be used to study exosome 

release from cancer cells co-cultured with hepatocytes 

pic: abc biopply 

Media and Partner for MSCs 

Gain more Control with Defined Media for MSCs 

Cell culture is a critical component of research, development 

and production in the life sciences. Cell culture media in 

particular, has a significant impact on the efficacy and 

sensitivity control of the cell culture microenvironment. 

PELOBiotech is strenghtening its leading position in the field 

of MSCs & GMP: We offer a special media for MSCs to our 

portfolio called CellCor TM , a serum-free chemically defined 

media composed of over 200 known compounds. 

Through 10 years of tenacious research, producer Xcell 

Therapeutics, South Korea, developed CellCor CD MSC 

products, which provide superior performance with the 

utmost focus safety and quality, assured by strict adherence to 

regulated standards following good manufacturing practices. 

CellCor does not contain any animal nor plant derived components, they are hPL free, 

and Exosome free, available in R&D and GMP quality. 

Scan QR-Code and get the Product sheet. 


NEW Products and Partners 

Alternatives to Matrigel 

We present our new partner Tissue Click. 

The company provides PhenoDrive which 

a the multifunctional nature, thereby 

unlike GelMatrix/Matrigel it can be used 

as a coating, in suspension or as a bio ink. 

This offers a simple and effective scale-up 

reducing experimental optimization 

pic: TisueClick 

and its time cost. 

Phenodrive vs GelMatrix/Matrigel at a glance 

GelMatrix/Matrigel 

Phenodrive 

Methods 

Coating, 

Encapsulation 

Coating, Encapsulation 

Suspension, bio-ink 

Temperature 4 °C Room Temperature 

Handeling 

(Viscosity) 

High 

Low 

Microscopy Opaque Transparent 

Composition Natural Synthetic 

Formulations 1 6 

Stability in culture 5 15 

Paper alert! 

Development of Scaffold-free vascularized pancreatic beta-islets in vitro 

models by the anchoring of cell lines to a biologand–functionalized 

gelatine substrate. 

Valeria Perugini et al. 2022. 

https://doi.org/10.1007/s10856-022-06658-3 

Abstract: 

The formation of bioengineered pancreatic islets through cell anchoring to a 

gelatine-based biomaterial, PhenoDrive-Y, is able to mimic the basement 

membrane of tissues . PhenoDrive-Y led human pancreatic ß Cell line and 

human umbilical endothelial cell lines structure which is similar to natural 

vascularized pancreatic islets. The study shows the potential of PhenoDrive-Y as 

a substrate for the development of bioengineered vascuklarized 

islets making it suitable for new therapeutics. 

9 

pancreatic 

YUMPU 

We have more than 

70 papers, flyers and 

brochures to read for 

free. https:// 

www.yumpu.com/kiosk/ 

pelobiotech


Organ/Cell based products 

Hepatocytes 

Primary human hepatocytes are the main cells in the liver, 

characterized by their cubical shape and abundant smooth 

endoplasmic reticulum. They exhibit eosinophilic cytoplasm, 

basophilic stippling, and round nuclei with dispersed chromatin. 

Hepatocytes form plates separated by sinusoids, which have 

fenestrated endothelial linings and are supported by reticulin. 

Kupffer cells and stellate cells are present in the sinusoids, 

performing phagocytosis and storing vitamin A, respectively. 

These hepatocytes “gold standard” for in vitro hepatic assays such 

as Drug Metabolism and Pharmacokinetics (DMPK). When 

choosing our primary human hepatocytes, you gain access to 

extensive donor information and large lot sizes of up to 1000 vials. 

The hepatocytes are evaluated for critical inductions such as 

CYP1A2, CYP2B6, and CYP3A4, and their metabolic activity is 

assessed for various essential enzymes which includes CYP450 

enzymes (1A2, 2B6, 2C8, 2C9, 2C19, 2D6, 3A), ECOD, SULT, 

UGT, and AO. The cells are available in plateable, suspension, and 

spheroid-qualified forms. 

By selecting these hepatocytes, you can explore the intricacies of liver function with confidence. 

With exceptional post-thaw viability (>85%) this allows for the study of xenobiotic metabolism, 

liver toxicity, cell biology, and host-pathogen interactions in a cost-effective manner. 

Read more about the use of hepatocytes in research by scanning/clicking the QR code: 

If you would like to purchase hepatocytes please contact us for more information. 

High-quality tissues by Anabios 

Precision-cut lung slices with exceptional RNA integrity (RIN 9.5) 

from normal and diseased tissue samples. These slices retain their 

functionality in ex-vivo assays, closely resembling fresh precisioncut 

lung slices. Additionally, we have included detailed donor 

information for enhanced research relevance. 

Other products include: Isolated cardiomyocytes (To predict druginduced 

proarrhythmia and inotropic risk), adult human dorsal root 

ganglia tissue and adult human CNS tissue 



PELONews: 199 People have opted to 

be cryopreserved, we do not know if 

revival is possible for them right now 

but our ThawSTAR CFT2 ® revives cells 

automatically with high efficiently and 

reproducible thawing. 

Enzymes for cell isolation & purification 

Different types of enzymes, such as collagenase, trypsin, dispase, 

and DNAse, are used in cell isolation processes. 

The choice of enzyme depends on the specific cell type and tissue 

being targeted. 

High-quality enzymes are essential for achieving high yield and 

functional cells during isolation. 

Quality enzymes ensure efficient and complete tissue digestion, 

leading to optimal cell dissociation. 

Enzymes with high specific activity and purity minimize non-specific 

cell damage and maximize cell viability. 

Contaminants or impurities in low-quality enzymes can negatively 

affect cell yield and compromise cell functionality. 

Well-characterized and standardized enzymatic activity ensures 

consistent and reproducible results. 

8. Consistent activity levels of enzyme batches allow for reliable and 

predictable cell isolation outcomes. 

To achieve exceptional and consistent outcomes, we highly recommend our partner, VitaCytes 

enzymes, for the isolation of cardiomyocytes, skin cells, liver cells, islet cells, adipose cells, and 

stem cells from humans, pigs, and rodents. Additionally, VitaCytes offers personalized enzyme 

solutions tailored to meet your specific requirements. 

11


Overview Extracellular Matrix (ECM) 

The Extracellular Matrix (ECM) is a vital component found in all tissues and organs, serving as a 

structural framework for cells and playing a crucial role in tissue development, differentiation, and 

maintenance. It provides essential biochemical and biomechanical signals necessary for proper tissue 

morphogenesis and homeostasis. Genetic abnormalities in ECM proteins can lead to a wide range of 

syndromes, varying in severity. As a result, targeting ECM molecules has become a significant focus 

in pharmacotherapy. Various diseases arise due to ECM abnormalities, including Osteoarthritis, 

cancer, genetic disorders like Marfan's syndrome, and even acquired conditions like coronary heart 

disease. 

Extracellular Matrices & Hydrogels products for R&D or GMP 

• Gelatin 

• Speed Coating Solution 

• PhenoDrive 

• Collagen (I, II, III, IV) 

• Fibronectin (human plasma derived; also available in GMP quality and virus inactivated) 

• Laminin/iMatrix 221 for Cardiomyocytes& Myoblasts, 411 for Endothelial Cells), 511 for ES/iPS 

cells) 

• Vitronectin 

• ECM Mixtures 

• Scilia ExtraGel 

• Alternatives to Matrigel and organ & cell culture derived ECMs 

Productlist ECMs: https://www.yumpu.com/en/document/read/62911690/portfolio-ecm-products 


New Products and Partners 

HydroSeev - 

Improving your 3D Cell culture 

HydroSeev, by our partner Seevix, is a 

formulation of a biopolymer with properties 

like that of spidersilk used as an additive for 

the betterment of mechanical properties, 

viability and function of 3D models. It is 

supplied as a stock suspension to 

supplement materials used for creating 3D 

Cellular models. 

Unique Structure and Properties 

Use SpheroSeev in drug discovery, stem cell 

research, cancer research and tissue 

engineering studies to reduce overall study 

costs. 

• Ready and easy-to-use 

• Minimum protocol adaption to switch 

from 2D or currently used 3D 

• high-porosity leading to bettter oxygen 

and nutrient access hence mimics 

function of vascularized tissue 

• Provides tissue-like environment for 

cells 

• extra strength and elasticity that 

provides mechanical cushioning and 

protection to individual cells within the 

spheroid. 

• Long shelf-life and sterile 

• Does not degrade over the course of 

the experiement. 

SpheroSeev 

Also find out about SpheroSeev 

->ECM-like environment for 

spheroid formation in a 

consistent and reproducible 

manner. 

13 

UltraGRO product line - 

Xeno-free human Platelet Lysate 

Platelets are one of the small components in 

blood circulation participating in primary 

hemostasis and thrombosis. Besides 

coagulation and wound healing, numerous 

research articles demonstrate the capability of 

human platelet lysate (hPL) in research, 

regenerative medicine and clinical applications. 

As a serum substitute, hPL has demonstrated 

higher expansion potency without 

compromising its differentiation characteristics 

and immunosuppression of T cells. 

AventaCell BioMedical Corp., a leading 

platelet lysate company, dedicating itself to 

developing novel human-derived products for 

cell culture. 

pic: 

AventaCell 

BioMedical 

Corp. 

UltraGRO-PURE meets your 

need for a consistent and reliable 

human protein based cell culture supplement 

that can support your cell production from 

research through to clinical trial use. 

UltraGRO TM – PURE GI GMP grade cell culture 

supplement is a gamma irradiated viral 

inactivated, fibrinogen depleted, xeno-free 

media supplement. 

Exosome-Depleted UltraGRO-PURE GI 

Research grade is gamma irradiated viral 

inactivated, exosome-depleted, xeno-free to 

support cell culture, especially for exosome 

secretion and isolation assays.

New Partners 


New Products 

High-quality tissues by Anabios 

1. Precision-cut lung slices with high RNA integrtiy (RIN 9.5) 

14 I www.pelobiotech.com 

2. Isolated cardiomyocytes (To predict drug-induced proarrhythmia 

and inotropic risk) 

3. Adult human dorsal toot ganglia tissue 

4. Adult human CNS tissue 

Apricell 3-in-1 plate 

To study co-culture 

Tumor invasion 

ECM study 

Drug screening 

Megarobo ECM & Organoid kits 

EXTRAGEL Reconstituted matrix hydrogel, equivalent to the gold-standard 

in the Industry. Suitable for stem cell maintenance and differentiation, Invivo 

xenograft generation, 3-D cell culture and to study angiogenesis. 

Organoid kits with media, cytokines and cells to create intestinal, lung, 

breast, liver, biliary, brain, vascular, and cardiac organoids

PELORecommends 

Hungry after a long lab day? Get back in shape with one of our favorite recipes. 

Ingredients: ½ kg fish (such as pompano), 1 tbsp lemon juice, 1 tsp turmeric powder, 1 tsp pepper 

powder, Salt (as required), ½ inch-sized ginger piece (finely minced), 2 garlic cloves (finely 

chopped), 1 medium-sized onion (sliced), 3-4 green chilies (slit), ¼ tsp turmeric powder, 1 tsp pepper, 

1 cup thin coconut milk, 1 cup thick coconut milk, 2 sprigs of curry leaves, 1 tbsp lemon juice, 1 

tomato (sliced into rounds), Salt (as required), Coconut oil (as required), 

Instructions: 

1. Clean and cut the fish into pieces. Marinate with lemon juice, pepper, salt, and turmeric 

powder for 15-20 minutes. 

2. In a kadai/pan, add a little oil and shallow fry the fish on both sides. Set it aside. 

3. In a separate pan, add oil and heat it over medium flame. Add curry leaves, ginger, garlic, and 

green chilies. Sauté for a minute until the raw smell subsides. 

4. Add the sliced onions and sauté until they turn translucent, without browning them. 

5. Add turmeric powder, pepper powder, and thin coconut milk. Close the lid and allow the curry 

to boil for 5-6 minutes on medium flame. When the oil starts separating, reduce the flame to 

low and add lemon juice. Mix well. 

6. Now add the fried fish, cover it with the gravy, and allow the curry to blend well with the fish. 

Bring it to a boil. Do not stir once the fish is added; instead, rotate the pan to mix the gravy. 

7. Add the thick coconut milk and cook on low flame for another 2 minutes, ensuring not to let the 

dish boil beyond this stage. Check for seasoning. 

8. Add sliced round tomatoes and more curry leaves. Close the lid and switch off the flame. Let the 

tomatoes cook in the remaining steam. 

9. Serve with Appam, Idiyappam, Chapati/Roti, Rice, or Bread. 

Notes: 

• Adding lemon juice is optional but highly recommended. You can skip it and add half a chopped 

tomato for extra tanginess. 

• Fishes like tilapia, pompano, salmon, kingfish, pomfret, red snapper, and pearl spot can be used. 

• Adjust the spiciness of the curry according to your taste by reducing green chilies. 

• Coconut oil is ideal for all authentic Kerala recipes, but any neutral oil (except for olive oil) would 

work. 

15

PELONews Spring/Summer 2023 2023 

PELOProductlist 

New 2023 list of human primary cells is available. 

Just scan and have a look! 

https://www.yumpu.com/en/document/ 

read/55613039/products-at-a-glance-2022 

PELOIntern 

We tasted the world-known Belgian beers and educated 

our taste buds to fine Belgian beer, our Sales 

Manager Dr. Gautier Arista brought back from home. 

Well now we know what distinguishes this amber 

liquid from other beers around the world. Do y- 

ou like to try too? Then come and see us in our offices! 

How to reach us 

If you need any further 

assistance or if you like 

what you see, tell us:-) 

PELOBiotech GmbH 

Klopferspitz 19 

82152 Planegg 

Germany 

+49 89 517 286 59 0 

info@pelobiotech.com 

www.pelobiotech.com 

Managing Directors: 

Dr. Peter Frost, 

Dr. Lothar Steeb 

You like what you 

see? 

Sign up for PELONews 

at 

www.pelobiotech.com 

or just fill out the online 

form 

We are happy to send you further information and 

product information on request. Just scan the QR code 

and fill out the form. 

Fill out our Portfolio and 

send it back to us. 

info@pelobiotech.com 

Or 

give us a call 

+49 89 517 286 59-0 

Welcome aboard! New faces at PELOBiotech 

Anne Osman 

Purchasing, Order 

Purchasing and Logistics 

Kevin Abraham 

Scientific content 

Ext:-23 

Marion Holzer 

Accounting 

Ext.:-0 

Quinn 

Stress manager 

Ext.:-11 

Ext.:-23

PELONEWS Spring/Summer 2023

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