PELONEWS Spring/Summer 2023

PELONews

PELONews Spring/Summer 2023 Overview Extracellular Matrix (ECM) The Extracellular Matrix (ECM) is a vital component found in all tissues and organs, serving as a structural framework for cells and playing a crucial role in tissue development, differentiation, and maintenance. It provides essential biochemical and biomechanical signals necessary for proper tissue morphogenesis and homeostasis. Genetic abnormalities in ECM proteins can lead to a wide range of syndromes, varying in severity. As a result, targeting ECM molecules has become a significant focus in pharmacotherapy. Various diseases arise due to ECM abnormalities, including Osteoarthritis, cancer, genetic disorders like Marfan's syndrome, and even acquired conditions like coronary heart disease. Extracellular Matrices & Hydrogels products for R&D or GMP • Gelatin • Speed Coating Solution • PhenoDrive • Collagen (I, II, III, IV) • Fibronectin (human plasma derived; also available in GMP quality and virus inactivated) • Laminin/iMatrix 221 for Cardiomyocytes& Myoblasts, 411 for Endothelial Cells), 511 for ES/iPS cells) • Vitronectin • ECM Mixtures • Scilia ExtraGel • Alternatives to Matrigel and organ & cell culture derived ECMs Productlist ECMs: https://www.yumpu.com/en/document/read/62911690/portfolio-ecm-products 12 I www.pelobiotech.com

New Products and Partners HydroSeev - Improving your 3D Cell culture HydroSeev, by our partner Seevix, is a formulation of a biopolymer with properties like that of spidersilk used as an additive for the betterment of mechanical properties, viability and function of 3D models. It is supplied as a stock suspension to supplement materials used for creating 3D Cellular models. Unique Structure and Properties Use SpheroSeev in drug discovery, stem cell research, cancer research and tissue engineering studies to reduce overall study costs. • Ready and easy-to-use • Minimum protocol adaption to switch from 2D or currently used 3D • high-porosity leading to bettter oxygen and nutrient access hence mimics function of vascularized tissue • Provides tissue-like environment for cells • extra strength and elasticity that provides mechanical cushioning and protection to individual cells within the spheroid. • Long shelf-life and sterile • Does not degrade over the course of the experiement. SpheroSeev Also find out about SpheroSeev ->ECM-like environment for spheroid formation in a consistent and reproducible manner. 13 UltraGRO product line - Xeno-free human Platelet Lysate Platelets are one of the small components in blood circulation participating in primary hemostasis and thrombosis. Besides coagulation and wound healing, numerous research articles demonstrate the capability of human platelet lysate (hPL) in research, regenerative medicine and clinical applications. As a serum substitute, hPL has demonstrated higher expansion potency without compromising its differentiation characteristics and immunosuppression of T cells. AventaCell BioMedical Corp., a leading platelet lysate company, dedicating itself to developing novel human-derived products for cell culture. pic: AventaCell BioMedical Corp. UltraGRO-PURE meets your need for a consistent and reliable human protein based cell culture supplement that can support your cell production from research through to clinical trial use. UltraGRO TM – PURE GI GMP grade cell culture supplement is a gamma irradiated viral inactivated, fibrinogen depleted, xeno-free media supplement. Exosome-Depleted UltraGRO-PURE GI Research grade is gamma irradiated viral inactivated, exosome-depleted, xeno-free to support cell culture, especially for exosome secretion and isolation assays.