PELONEWS Spring/Summer 2023

PELONews

PELONews Spring/Summer 2023 Overview Extracellular Vesicles Table 1. Comparison of Cell Culture Factors for Cell Growth and Exosome Studies. Adapted from Liau et al, Int J Mol Sci, 2021 Factors FBS hPL Defined Media EXO Free Media Pros Abundant and accessible Easy to produce Well-defined components Exosome-depleted and ideal for isolation Suitable for most cells Suitable for most cells No batch-to-batch variation No batch-to-batch variation Supports cell survival and proliferation Supports cell survival and proliferation No risk of xenogeneic/ allogeneic protein contamination No risk of xenogeneic/allogeneic protein contamination Widely used in studies and publications No risk of xenogenic immune reaction; can be produced using autologous blood No unintended interaction with test substances No unintended interaction with test substances Cons Components are illdefined Components are illdefined Not available for certain cells Not available for certain cells Batch-to-batch variation Batch-to-batch variation (reduced by pooling) Some media require extra coating for cell attachment Some media require extra coating for cell attachment Potential contamination (prions, mycoplasma, bacteria, fungi) Unintended interaction with test substances More expensive compared to alternatives More expensive compared to alternatives Risk of animal protein contamination in clinical usage Few distributors Time-consuming and difficult to develop Time-consuming and difficult to develop Unintended interaction with test substances Potential allogenic immune response Higher particle count and presence of exosomes Ethical concerns with animal welfare Risk of transmitting human viruses Multitude of exosomes not suitable for isolation or studies Multitude of exosomes not suitable for isolation or studies 4 I www.pelobiotech.com

Overview Extracellular Vesicles Media for cell and gene therapy applications Media for Stem cell maintenance and proliferation CellCor CD MSC Choosing the right media is crucial for achieving optimal growth of human MSCs with the desired characteristics. CellCor CD MSC is an excellent choice for controlling culture conditions, as it is made up of chemically defined components. This product can maintain stable stemness, differentiation, and marker expression throughout subculturing, up to the second half. Furthermore, it supports low aging and high genetic stability of cells and is suitable for MSCs from different tissues such as Adipose, Bone marrow, Cord blood, and Wharton jelly. It can also be used for the production and research of stem cell-derived exosomes. Scan the QR-Code and get the Product sheet. 5 New Products !