PeloNews with Focus on Liver/Islets

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New Products NEW: DNs-Rh: DNs-Rh is a novel fluorogenic substrate for GSTs (Glutathione S-Transferases). Compared to conventional reagents, DNs-Rh can be used for live cell imaging ong>withong> high specificity and sensitivity discovered by Dr. Hiroshi Abe, Nagoya University. As many studies suggested, expression level of GSTs is significantly increased in cancer cells, GSTs are considered as anti-cancer drugresistant enzymes in malignant cancer cells through the neutralization of drugs. To understand biological functions of GSTs, tools for monitoring GST activity are very important. Although several reagents including CDNB (conventional GSTs probe) for this purpose have been developed, no tool to measure intracellular GST activity was commercially available. DNs-Rh is a rhodamine 110 derivative which protected by DNs (dinitrobenzenesulfonamide). This probe emits very low fluorescence (quantum yield = 0.0007) at normal state. After deprotected by thiols, rhodamine 110 is released and exhibits strong fluorescence (quantum yield = 0.645, S/N ratio ~900). DNs-Rh can be used as a fluorogenic GST activity assay probe. An important advantage of this probe is high cellpermeability and this probe is applied to intracellular GST activity under live cell condition. As DNs group is a well characterized substrate for various types of GST members, DNs-Rh is able to monitor pan- GST activity both in cell and in vitro. More infos: https://www.funakoshi.co.jp/exports_contents/81304 NEW: These cells are endothelial-like cells in a sponge-like connective tissue located near the front of the eye and are responsible for regualting eye pressure by controlling drainage of fluid into tubes that flow into the bloodstream. Damage and dysfunction of TM have clinical significance. For instance, hypoxia increases DNA methylation, accompanied by altered gene expression, whereas during the normal aging process, TM number decreases, and senescent cells accumulate. In Glaucoma, a leading cause of irreversible blindness, decreased fluid outflow causes an elevation of intraocular pressure and progressive loss of retinal ganglion cells. Physical changes to the TM include increased fibrosis, fibronectin accumulation, and expression of ECM cross-linking enzymes. Choose your Trabecular Meshwork Cells listed below or contact us for custom human or animal ocular cell types for your research projects or applications: Ask Peter for more details sales@pelobiotech.com Canine Trabecular Meshwork Cells: CnTMC, Human Trabecular Meshwork Cells: HnTMC, Porcine Trabecular Meshwork Cells: PnTMC

More New Products PELOBiotech‘s Premium-brand Cellovations® launched NEW Media Human Hepatocyte Medium - 2D (HHM-2D) and Human Hepatocyte Medium - 2D (HHM-3D) HHM-2D and HHM-3D are complete media formulated for optimal cultivation of all hepatocytes, human and animal, etiher to grow in 2D after plating or to build spheroids (3D). They are serum-free and growth factor containing culture media and contain essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, trace minerals together ong>withong> the growth factor containing supplements. They contain NO FCS. The media are delivered as Kit ong>withong> separate supplements. The media may be used for functionel long-term cultivation of hepatocytes to study hepatotocxity of chemicals and drug-drug interactions, or in hepatocyte bioreactors. Please be aware that hepatocytes do not proliferate. They may be used to study hepatotocxity of chemicals and drug-drug interactions, or in hepatocyte bioreactors. Please ask Lothar for further Details. Cat. #: PB-MH-053-5990-2D or PB-MH-053-5990-3D