Dannylo Wesley Nóbrega de Sousa - Uninove

Pilot study of gene expression analysis of kinin B1 and B2 receptors in adipose tissue
depots in Cushing’s syndrome subjects.
Estudo piloto da análise da expressão gênica de receptores de cininas em depósitos
adiposos de sujeitos com Síndrome de Cushing.
Running Title: Kinin expression in adipose depots in Cushing’s syndrome.
Título abreviado: Expressão de cininas em depósito adiposo em Síndrome de Cushing.
Abstract
Introduction: Glucocorticoids (GC) play an important role in determining the distribution
of adipose tissue and metabolism. Individuals with endogenous or exogenous
hypercortisolism develop a pattern of central obesity, which is reversible after treatment or
withdrawal of glucocorticoids. The mechanisms involved in the distribution of adipose
tissue mediated by glucocorticoids are not fully understood. The objective of this study was
to quantify and evaluate the effects of chronic exposure to hypercortisolism on kinin B1
and B2 receptors expression in subcutaneous and visceral fat deposition in patients with
Cushing's syndrome. Method: Samples of subcutaneous adipose tissue (SAT) and visceral
adipose tissue (VAT) were obtained during elective abdominal surgery in female patients
with Cushing's syndrome (n = 5), in which the body mass index (BMI), waist
circumference (WC) and salivary cortisol (SC) were determined. The mRNA expression of
B1 and B2 receptors were quantified by Real-time PCR. Results: The expression of kinin
B1 receptor was markedly increased in VAT than in SAT of patients with Cushing’s
syndrome (p