Dannylo Wesley Nóbrega de Sousa - Uninove

ABSTRACT
Glucocorticoids (GC) play an important role in determining the distribution of
adipose tissue and metabolism. Individuals with Cushing's syndrome (CS) show a
pattern of central obesity, related to excessive levels of plasma glucocorticoids
exogenous or endogenous origin which is reversible after treatment or withdrawal
of corticosteroids. The mechanisms involved in the distribution of adipose
tissue mediated by glucocorticoids are not fully understood. The objective of
this study was to quantify and evaluate the effects of chronic exposure to
hypercortisolism in the gene expression of components of the kallikrein-kinin
fat deposits in patients with Cushing's Syndrome. Samples of visceral (VAT)
and subcutaneous adipose tissue (SAT) were obtained during elective abdominal
surgery for female patients with Cushing's Syndrome (n = 9), in which the body
mass index (BMI), waist circumference (WC) and salivary cortisol (CTS) were
previously determined. The mRNA expression of tissue kallikrein and
the kinin B1 and B2 receptors were quantified by real-time PCR. The mRNA
expression of kinin B2 receptor in SAT and VAT of Cushing's patients was similar
in both compartments (p> 0.05). The expression of kinin B1 receptor was
reduced in SAT when compared to VAT of patients with Cushing (p