TDS pSELECT-zeo-hSEAP-CpG-free : 20 µg - InvivoGen
TDS pSELECT-zeo-hSEAP-CpG-free : 20 µg - InvivoGen
TDS pSELECT-zeo-hSEAP-CpG-free : 20 µg - InvivoGen
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BspLU11I (3788)<br />
PacI (3782)<br />
PstI (3775)<br />
SdaI (3775)<br />
ApaLI (4102)<br />
EagI (4538)<br />
NotI (4538)<br />
SwaI (4530)<br />
PacI (4522)<br />
pMB1 ori<br />
SgfI (11)<br />
PvuI (11)<br />
Psp1406I (<strong>20</strong>5)<br />
HindIII (246)<br />
Bsu36I (293)<br />
KasI (536)<br />
AgeI (553)<br />
BstEII (556)<br />
NcoI (561)<br />
Bsp1<strong>20</strong>I (571)<br />
Bsu36I (608)<br />
hEF1-HTLV prom<br />
<strong>hSEAP</strong> <strong>CpG</strong>-<strong>free</strong><br />
SnaBI (3489)<br />
hCMV enh<br />
<strong>pSELECT</strong>-<strong>zeo</strong>-<strong>hSEAP</strong><br />
(4701 bp)<br />
SphI (1091)<br />
SpeI (3359)<br />
SacI (3265)<br />
AseI (3<strong>20</strong>5)<br />
MscI (3145)<br />
SfiI (3143)<br />
BssHII (3108)<br />
BsrBI (3097)<br />
XmaI (3056)<br />
SgrAI (3030)<br />
125<br />
hCMV prom<br />
EM7<br />
Sh ble<br />
(h) ßglobin p(A)<br />
SV40 p(A)<br />
EagI (2813)<br />
ApaLI (2799)<br />
DraIII (2798)<br />
SwaI (2617)<br />
SspI (2602)<br />
EcoRI (2361)<br />
HpaI (2267)<br />
NheI (2127)<br />
MscI (2135)<br />
StuI (1849)<br />
Bsp1<strong>20</strong>I (1957)<br />
HpaI (1662)<br />
BsrGI (1330)<br />
StuI (1397)<br />
PshAI (1483)<br />
TECHNICAL SUPPORT<br />
Toll <strong>free</strong> (US): 888-457-5873<br />
Outside US: (+1) 858-457-5873<br />
Europe: +33 562-71-69-39<br />
E-mail: info@invivogen.com<br />
Website: www.invivogen.com<br />
3950 Sorrento Valley Blvd. Suite 100<br />
San Diego, CA 92121 - USA
1<br />
101<br />
<strong>20</strong>1<br />
301<br />
401<br />
PvuI (11)<br />
SgfI (11)<br />
GGATCTGCGATCGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACG GGTGCCTA<br />
GAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCC<br />
Psp1406I (<strong>20</strong>5) HindIII (246) Bsu36I (293)<br />
GTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGCTGAAGCTTCGAGGGGCTCGCATCTCTCCTTCACGCGCCCGCCGCCCTACCTGAGGCC<br />
GCCATCCACGCCGGTTGAGTCGCGTTCTGCCGCCTCCCGCCTGTGGTGCCTCCTGAACTGCGTCCGCCGTCTAGGTAAGTTTAAAGCTCAGGTCGAGACC<br />
GGGCCTTTGTCCGGCGCTCCCTTGGAGCCTACCTAGACTCAGCCGGCTCTCCACGCTTTGCCTGACCCTGCTTGCTCAACTCTACGTCTTTGTTTCGTTT<br />
NcoI (561)<br />
BstEII (556)<br />
KasI (536) AgeI (553)<br />
Bsp1<strong>20</strong>I (571)<br />
501 TCTGTTCTGCGCCGTTACAGATCCAAGCTGTGACCGGCGCCTACCTGAGATCACCGGTCACCATGGTATTGGGCCCCTGTATGCTACTGTTACTTTTATT<br />
1 M V L G P C M L L L L L L<br />
Bsu36I (608)<br />
601 ACTGGGCCTGAGGTTACAGCTGAGCCTTGGGATAATACCTGTTGAAGAGGAGAACCCTGACTTCTGGAATAGAGAAGCAGCTGAAGCTCTGGGAGCAGCA<br />
13 L G L R L Q L S L G I I P V E E E N P D F W N R E A A E A L G A A<br />
701 AAGAAACTACAACCTGCCCAGACAGCAGCCAAGAACCTGATAATCTTCCTTGGAGATGGGATGGGGGTCAGCACTGTCACTGCTGCCAGAATCCTGAAAG<br />
47 K K L Q P A Q T A A K N L I I F L G D G M G V S T V T A A R I L K<br />
801 GCCAGAAGAAGGACAAGTTAGGACCAGAGATACCCCTAGCAATGGACAGATTCCCATATGTAGCCCTTTCCAAGACCTACAATGTAGACAAGCATGTGCC<br />
80 G Q K K D K L G P E I P L A M D R F P Y V A L S K T Y N V D K H V P<br />
901 TGACTCAGGGGCCACTGCCACAGCTTACCTGTGTGGAGTCAAGGGGAACTTCCAAACAATTGGTCTGAGTGCTGCTGCAAGGTTCAACCAGTGCAACACA<br />
113 D S G A T A T A Y L C G V K G N F Q T I G L S A A A R F N Q C N T<br />
SphI (1091)<br />
1001 ACCAGAGGGAATGAGGTGATTTCTGTGATGAACAGAGCTAAGAAAGCTGGGAAGTCAGTAGGGGTAGTGACCACCACAAGGGTCCAGCATGCAAGCCCTG<br />
147 T R G N E V I S V M N R A K K A G K S V G V V T T T R V Q H A S P<br />
1101 CTGGGACATATGCTCACACTGTAAACAGGAACTGGTATTCTGATGCTGATGTGCCAGCCTCAGCAAGACAGGAGGGGTGCCAAGATATAGCCACCCAGCT<br />
180 A G T Y A H T V N R N W Y S D A D V P A S A R Q E G C Q D I A T Q L<br />
1<strong>20</strong>1 GATTTCCAACATGGACATAGATGTGATATTGGGAGGGGGCAGGAAGTATATGTTCAGAATGGGGACTCCTGACCCTGAGTACCCTGATGATTACTCTCAA<br />
213 I S N M D I D V I L G G G R K Y M F R M G T P D P E Y P D D Y S Q<br />
BsrGI (1330) StuI (1397)<br />
1301 GGTGGCACAAGGTTAGATGGAAAGAATCTTGTACAGGAGTGGCTTGCAAAGAGGCAGGGGGCTAGGTATGTTTGGAACAGGACTGAGCTGATGCAGGCCT<br />
247 G G T R L D G K N L V Q E W L A K R Q G A R Y V W N R T E L M Q A<br />
PshAI (1483)<br />
1401 CTCTGGACCCATCTGTCACCCATTTGATGGGCCTCTTTGAGCCTGGTGACATGAAGTATGAGATACACAGGGACTCAACATTGGACCCCAGTCTAATGGA<br />
280 S L D P S V T H L M G L F E P G D M K Y E I H R D S T L D P S L M E<br />
1501 AATGACTGAGGCTGCTCTGAGACTCCTTTCAAGGAACCCAAGGGGTTTCTTCTTATTTGTGGAGGGTGGCAGAATTGATCATGGGCACCATGAGAGCAGA<br />
313 M T E A A L R L L S R N P R G F F L F V E G G R I D H G H H E S R<br />
HpaI (1662)<br />
1601 GCCTACAGAGCACTCACTGAAACAATAATGTTTGATGATGCAATTGAGAGGGCAGGTCAGTTAACATCTGAGGAGGACACCCTATCCCTAGTCACAGCAG<br />
347 A Y R A L T E T I M F D D A I E R A G Q L T S E E D T L S L V T A<br />
1701 ACCACTCTCATGTATTCTCTTTTGGGGGGTATCCCTTGAGGGGCTCCTCAATCTTTGGACTGGCTCCTGGCAAAGCAAGAGACAGAAAGGCTTACACTGT<br />
380 D H S H V F S F G G Y P L R G S S I F G L A P G K A R D R K A Y T V<br />
StuI (1849)<br />
1801 TCTGTTGTATGGCAATGGCCCAGGCTATGTGCTGAAGGATGGTGCAAGGCCTGATGTAACTGAGTCTGAAAGTGGCAGCCCTGAATACAGGCAGCAGTCA<br />
413 L L Y G N G P G Y V L K D G A R P D V T E S E S G S P E Y R Q Q S<br />
Bsp1<strong>20</strong>I (1957)<br />
1901 GCAGTGCCCTTGGATGAGGAGACTCATGCAGGGGAGGATGTGGCAGTGTTTGCAAGGGGCCCTCAAGCTCACTTAGTTCATGGAGTGCAGGAGCAAACCT<br />
447 A V P L D E E T H A G E D V A V F A R G P Q A H L V H G V Q E Q T<br />
<strong>20</strong>01 TTATTGCCCATGTGATGGCCTTTGCAGCTTGTCTGGAACCCTACACAGCCTGTGACCTTGCTCCCCCAGCAGGGACCACAGATGCTGCTCACCCAGGAAG<br />
480 F I A H V M A F A A C L E P Y T A C D L A P P A G T T D A A H P G R<br />
MscI (2135)<br />
NheI (2127)<br />
2101 GAGCAGGTCCAAGAGGCTTGATTAAAGCTAGCTGGCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATG<br />
513 S R S K R L D •<br />
HpaI (2267)<br />
2<strong>20</strong>1 CTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG<br />
2301<br />
2401<br />
2501<br />
2601<br />
EcoRI (2361)<br />
GTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGAATTCTAAAATACAGCATAGCAAAACTTTAACCTCCAAA<br />
TCAAGCCTCTACTTGAATCCTTTTCTGAGGGATGAATAAGGCATAGGCATCAGGGGCTGTTGCCAATGTGCATTAGCTGTTTGCAGCCTCACCTTCTTTC<br />
SspI (2602)<br />
ATGGAGTTTAAGATATAGTGTATTTTCCCAAGGTTTGAACTAGCTCTTCATTTCTTTATGTTTTAAATGCACTGACCTCCCACATTCCCTTTTTAGTAAA<br />
SwaI (2617)<br />
ATATTCAGAAATAATTTAAATACATCATTGCAATGAAAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTT
ApaLI (2799)<br />
DraIII (2798)<br />
2701 AGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTCTAGCTTATCCTCAGTCCTGCTCCTCTGCCACAAAGT<br />
125 • D Q E E A V F H<br />
EagI (2813)<br />
2801 GCACGCAGTTGCCGGCCGGGTCGCGCAGGGCGAACTCCCGCCCCCACGGCTGCTCGCCGATCTCGGTCATGGCCGGCCCGGAGGCGTCCCGGAAGTTCGT<br />
116 V C N G A P D R L A F E R G W P Q E G I E T M A P G S A D R F N T<br />
2901 GGACACGACCTCCGACCACTCGGCGTACAGCTCGTCCAGGCCGCGCACCCACACCCAGGCCAGGGTGTTGTCCGGCACCACCTGGTCCTGGACCGCGCTG<br />
83 S V V E S W E A Y L E D L G R V W V W A L T N D P V V Q D Q V A S<br />
SgrAI (3030) XmaI (3056) BsrBI (3097)<br />
3001 ATGAACAGGGTCACGTCGTCCCGGACCACACCGGCGAAGTCGTCCTCCACGAAGTCCCGGGAGAACCCGAGCCGGTCGGTCCAGAACTCGACCGCTCCGG<br />
49 I F L T V D D R V V G A F D D E V F D R S F G L R D T W F E V A G A<br />
SfiI (3143)<br />
BssHII (3108) MscI (3145)<br />
3101 CGACGTCGCGCGCGGTGAGCACCGGAACGGCACTGGTCAACTTGGCCATGATGGCCCTCCTATAGTGAGTCGTATTATACTATGCCGATATACTATGCCG<br />
16 V D R A T L V P V A S T L K A M<br />
AseI (3<strong>20</strong>5) SacI (3265)<br />
3<strong>20</strong>1 ATGATTAATTGTCAAAACAGCGTGGATGGCGTCTCCAGCTTATCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACC<br />
3301<br />
3401<br />
3501<br />
3601<br />
3701<br />
3801<br />
3901<br />
4001<br />
4101<br />
4<strong>20</strong>1<br />
4301<br />
4401<br />
4501<br />
SpeI (3359)<br />
GCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTACTAGTCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGA<br />
SnaBI (3489)<br />
CTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCAT CATGGTAATAGCGATGACTAATACGTAGATGTACT<br />
GCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATA<br />
CACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACTATGGGAACATACGTCATT<br />
PacI (3782)<br />
PstI (3775)<br />
SdaI (3775)<br />
BspLU11I (3788)<br />
ATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCCTGCAGGTTAATTAAGAACATGTGAGCAAA<br />
AGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGT<br />
CAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT<br />
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTG<br />
ApaLI (4102)<br />
TGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCA<br />
GCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTG<br />
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTG<br />
CAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGG<br />
EagI (4538)<br />
PacI (4522) SwaI (4530) NotI (4538)<br />
ATTTTGGTCATGGCTAGTTAATTAACATTTAAATCAGCGGCCGCAATAAAATATCTTTATTTTCATTACATCTGTGTGTTGGTTTTTTGTGTGAATCGTA<br />
4601<br />
4701<br />
ACTAACATACGCTCTCCATCAAAACAAAACGAAACAAAACAAACTAGCAAAATAGGCTGTCCCCAGTGCAAGTGCAGGTGCCAGAACATTTCTCTATCGA<br />
A
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