korica 5-6 2009.cdr - Institut za higijenu i tehnologiju mesa

Milićević Dragan i dr.
Prisustvo plesni i mikotoksina u hrani za ishranu svinja – značaj u proceni rizika
one was genus Penicillium (94,4%). The moulds from genus Fusarium were isolated in 55,5% and Paecilomyces in 44,4% of
the samples from investigated localities. Other fungi from the genera Aspergillus (22%), Mycor (11,1%) and Alternaria (5,5%)
were represented in a lesser amount. Polymerase chain reaction (PCR) was performed on 18 isolates of the DNA belonging to
families Penicillium and Aspergillus. The sequences of PCR reaction products in three samples were compared with nucleotide
sequences of genes for polyketide synthase gene (PKS) from Penicillium dance and found that the samples posessed PKS
sequence. The traditional methods for identifi cation of ochratoxin-producing fungi are time-consuming and labor-intensive.
Rapid and specifi c detection of ochratoxin-producing fungi is important for ensuring microbiological quality and safety of feed
and food.
Key words: moulds, mycotoxins, feedingstuffs, PCR, risk assessment.
Rad primljen: 26.10.2009.
Rad ispravljen: 14.12.2009.
Rad prihvaćen: 15.12.2009.
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