peqGOLD Blood RNA Kit - Peqlab

TROUBLESHOOTING TIPSProblemLittle or no RNAelutedLikely causeRNA remains on thecolumnSuggestion• Repeat elution.• Pre-heat RNase-free water to70 °C prior to elution.• Incubate column for 5 min withwater prior to centrifugation.Column isoverloaded• Reduce quantity of startingmaterial.Clogged column Incomplete lysis • completely resuspend pellet.• Reduce amount of startingmaterialDegraded RNAProblem indown-streamapplicationsSource • Do not store blood sample priorto extraction unless they arelysed first.• Follow protocol closely, andwork quickly.RNase contamination • Ensure not to introduce RNaseduring the procedure.• Check buffers for RNasecontamination.Salt carry-overduring elution• Ensure Wash Buffer IIConcentrate has been dilutedwith 4 volumes of 100% ethanolas indicated on bottle.• 1 x Wash Buffer II must bestored and used at roomtemperature.• Repeat wash with RNA WashBuffer II.PCR inhibitors • Reduce volume of sample• For complete lysis of red bloodcells increase incubation withLysis Buffer ERDNAcontamination• Digest with RNase-free DNaseand incubate at 37 °C for5 min.peqGOLD Blood RNA Kit Art.-Nr.: 12-6814/12-6614PEQLAB_v0314 19