BioSep: - sonosep

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Fresh feed Recirculation pump • speed ±3 x harvest flow Fresh feed Clarified culture medium Harvest pump • variable speed with remote control input • variable speed with remote control input and prime rate reverse action Clarified culture medium Concentrated cell recycle Concentrated cell recycle Harvest Cell suspension Harvest Prime rate reverse pump Cell suspension Pumps and other hardware to complete a perfusion set-up The harvest pump is a variable speed pump. The pump is stopped (remotely) by the BioSep controller during field off times, facilitating the settling of aggregates. This means that the harvest flow is stopped for a few seconds, arresting the flow in the chamber. The stop time of a few seconds will affect the overall harvest flow rate by a small amount. This pump can be controlled directly by a connecting cable between the BioSep controller and the pump. In cultures of sticky cells or too large aggregates, the variable speed pump with prime rate reverse action (code Z288010020) is a recommended option.The pump has the capability to reverse the harvest flow at full pump speed (prime), during field off time. Sticky cells can adhere to the glass of the resonator chamber. By reversing the flow at full speed during field off times (for 3 sec) these cells will get a small push back into the bioreactor. Reversing the flow every 10 min will result in less adherence of cells in the BioSep chamber. A typical BioSep set-up BioSep 10L: • holder to fit resonance chamber into the headplate • suction tube for recirculation loop • recirculation pump • harvest pump (variable speed / remote controlled) • fresh medium inlet and pump (e.g. controlled via level controller) BioSep 50L: • diptube for return of cells from chamber. (The diptube should be provided with the proper headplate connector and a 0.5” TC to install the resonance chamber). • suction tube for recirculation loop • recirculation pump • harvest pump (variable speed / remote controlled) • fresh medium inlet and pump (e.g. controlled via level controller) Various adapters are available to fit the BioSep 50L to any type or brand of bioreactor. Adapter M18-0.5”SC-9.53 Adapter PG13.5-0.5”SC-9.53 Adapter D27-0.5”SC-9.53
BioSep 200L: Scale-up of acoustic perfusion There are several options for connecting the return outlet to the reactor, depending on the chosen way of sterilization.The BioSep chamber is placed on a support above the reactor preferably straight above the return tube in the top plate to ensure smooth return of the recirculation medium preventing sedimentation of cells in the tubing. Additional hardware for a BioSep 200L set-up: • BioSep chamber and controller • BioSep support • Pumps, pump head, pump tubing (harvest, recirculation, feed) • Water bath (temperature control of resonance chamber) • Contained additions ) • Sensors (pH, DO, T) Optional • Safety clamps pump tubing Example P&ID for a fully contained BioSep in S.I.P. References: 1. H. Bierau, A. Perani, M. Al-Rubeai, A.N. Emery. A comparison of intensive cell culture bioreactors operating with Hybridomas modified for inhibited apoptotic response. Journal of biotechnology 62, 195-207, 1998. 2. Gorenflo, V.M., Smith, L, Dedinsky, B., Persson, B. and Piret, J.M. Scale-up and Optimization of an Acoustic Filter for 200 L/day Perfusion of a CHO Cell Culture. Biotechnology and Bioengineering, vol 80, no. 4, nov. 2002 3. Miller, A.O.A. Combing cell culture & process operation. Sonoperfusion allows direct feed with expanded-bed chromatography. GEN Vol. 21, p29, 2001. 4. Pui, P.W.S., Trampler, F., Sonderhoff, S.A., Groeschl, M., Kilburn, D.G. and Piret, J.M. “Batch and Semi-Continuous Aggregation and Sedimentation of Hybridoma Cells by Acoustic Resonance Fields”, Biotechnol. Prog. 11: 146-152, 1995. 5. Ryll, T., Dutina, G., Reyes, A., Gunson, J., Krummen, L., Etcheverry, T., Performance of small-scale CHO perfusion cultures using an acoustic cell filtration device for cell retention: Characterization of separation efficiency and impact of perfusion on product quality, Biotechnol Bioeng 69: 440-449, 2000. 6. Trampler, F., Sonderhoff, S.A., Pui, P.W.S., Kilburn, D.G. and Piret, J.M. “Acoustic Cell Filter for High Density Perfusion Culture of Hybridoma Cells”, Bio/Technol. 12: 281-284, 1994. 7. S.M.Woodside, B.D. Bowen, J.M. Piret. Mammalian cell retention devices for stirred perfusion bioreactor. Cytotechnology 28, 163-175, 1998. 8. Zhang, J., A. Collins, M. Chen, I. Knyazev and R. Gentz “High-density perfusion culture of insect cells with a BioSep ultrasonic filter”, Biotechnol Bioeng 59: 351-359, 1998. A 100L working volume bioreactor with a BioSep 200L chamber and support. The BioSep technology is patented (US 5.626.767)
Page 1 and 2: Technical Data Sheet STS90 Duration
Page 3 and 4: The BioSep chamber is mounted above
Page 5 and 6: Due to their higher acoustic contra
Page 7 and 8: APS 990 BioSep Controller for BioSe
Page 9: BioSep Chamber 200L Mechanical: Mat

BioSep: - sonosep

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